Restriction map for
pFN21AE0324
(Circular) (Six-base) MAPSORT of: pFN21AE0324.seq Check: 2648 from: 1 to: 6559
pFN21AE0324 6559 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4369 278
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2417 3902 1937
Size: 480 1485 4594
Fragments arranged by size:
4594 1485 480
Acc65I G'GTAC_C
Cuts at: 3880 3880
Size: 6559
AclI AA'CG_TT
Cuts at: 1563 4686 5059 1563
Size: 3123 373 3063
Fragments arranged by size:
3123 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 3177 3293 3474 4622 6006 6274 1632
Size: 848 697 116 181 1148 1384 268 1917
Fragments arranged by size:
1917 1384 1148 848 697 268 181 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6362
AflIII A'CryG_T
Cuts at: 1203 5458 6435 1203
Size: 4255 977 1327
Fragments arranged by size:
4255 1327 977
AgeI A'CCGG_T
Cuts at: 5390 5390
Size: 6559
AhdI GACnn_n'nnGTC
Cuts at: 5288 5288
Size: 6559
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6559
AlwNI CAG_nnn'CTG
Cuts at: 4302 5874 4302
Size: 1572 4987
ApaI G_GGCC'C
Cuts at: 2520 2520
Size: 6559
ApaLI G'TGCA_C
Cuts at: 4616 5772 4616
Size: 1156 5403
ApoI r'AATT_y
Cuts at: 1493 2087 3348 3443 3868 4094 4246 6355 1493
Size: 594 1261 95 425 226 152 2109 1697
Fragments arranged by size:
2109 1697 1261 594 425 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 5113 160
Size: 872 4081 1606
Fragments arranged by size:
4081 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 3884 1870
Size: 73 166 1775 4545
Fragments arranged by size:
4545 1775 166 73
BamHI G'GATC_C
Cuts at: 2022 3889 4256 2022
Size: 1867 367 4325
Fragments arranged by size:
4325 1867 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3132 3880 5335 618
Size: 325 217 627 1345 748 1455 1842
Fragments arranged by size:
1842 1455 1345 748 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2478 2520 3773 3878 1391
Size: 1087 42 1253 105 4072
Fragments arranged by size:
4072 1253 1087 105 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 928
Size: 971 161 5427
Fragments arranged by size:
5427 971 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4750 4784 1903
Size: 34 2813 34 3678
Fragments arranged by size:
3678 2813 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3750 4451 5661 3750
Size: 701 1210 4648
Fragments arranged by size:
4648 1210 701
BclI T'GATC_A
Cuts at: 1548 2593 1548
Size: 1045 5514
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 6559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5170 136
Size: 107 122 71 631 605 3498 1525
Fragments arranged by size:
3498 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6554 6554
Size: 6559
BlpI GC'TnA_GC
Cuts at: 3976 3976
Size: 6559
Bme1580I G_kGCm'C
Cuts at: 1790 2520 4620 5776 1790
Size: 730 2100 1156 2573
Fragments arranged by size:
2573 2100 1156 730
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3091 3693 4290
Size: 416 53 83 399 24 1674 602 597
Cuts at: 4290 5248 442
Size: 958 1753
Fragments arranged by size:
1753 1674 958 602 597 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2702 5219 1239
Size: 1463 2517 2579
Fragments arranged by size:
2579 2517 1463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2658 3171 3749 4684 5549 5847 2658
Size: 513 578 935 865 298 3370
Fragments arranged by size:
3370 935 865 578 513 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 5222 882
Size: 617 885 2838 2219
Fragments arranged by size:
2838 2219 885 617
BsaAI yAC'GTr
Cuts at: 493 1204 3748 4350 493
Size: 711 2544 602 2702
Fragments arranged by size:
2702 2544 711 602
BsaBI GATnn'nnATC
Cuts at: 6553 6553
Size: 6559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4366 4748 5383 275
Size: 53 83 186 936 2833 382 635 1451
Fragments arranged by size:
2833 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4992 5390 5664 5811 6222 4992
Size: 398 274 147 411 5329
Fragments arranged by size:
5329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6334
Fragments arranged by size:
6334 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2661 1186
Size: 1363 112 5084
Fragments arranged by size:
5084 1363 112
BseYI C'CCAG_C
Cuts at: 5762 6258 5762
Size: 496 6063
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5986
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4009 4770 5798 1540
Size: 275 186 2008 761 1028 2301
Fragments arranged by size:
2301 2008 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 3773 4620 4705 5776 2478
Size: 1295 847 85 1071 3261
Fragments arranged by size:
3261 1295 1071 847 85
BsmI GAATG_Cn'
Cuts at: 2266 4070 4163 2266
Size: 1804 93 4662
Fragments arranged by size:
4662 1804 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 2520 3773 3878 4620 4705 5776
Size: 399 688 42 1253 105 742 85 1071
Cuts at: 5776 1391
Size: 2174
Fragments arranged by size:
2174 1253 1071 742 688 399 105 85
42
BspEI T'CCGG_A
Cuts at: 6222 6222
Size: 6559
BspHI T'CATG_A
Cuts at: 4448 6178 4448
Size: 1730 4829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3237 3603 3914 844
Size: 2393 366 311 3489
Fragments arranged by size:
3489 2393 366 311
BsrBI CCG'CTC
Cuts at: 1394 1823 4446 1394
Size: 429 2623 3507
Fragments arranged by size:
3507 2623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5054 5228 65
Size: 1183 3806 174 1396
Fragments arranged by size:
3806 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5203 5390 1700
Size: 110 3393 187 2869
Fragments arranged by size:
3393 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3124 3834 96
Size: 3028 710 2821
Fragments arranged by size:
3028 2821 710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6559
BssSI C'ACGA_G
Cuts at: 4619 5631 4619
Size: 1012 5547
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6559
BstBI TT'CG_AA
Cuts at: 5363 5363
Size: 6559
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6559
BstYI r'GATC_y
Cuts at: 1972 2022 3889 4256 4641 4658 6110 6196 6208
Size: 50 1867 367 385 17 1452 86 12
Cuts at: 6208 6554 1972
Size: 346 1977
Fragments arranged by size:
1977 1867 1452 385 367 346 86 50
17 12
BstZ17I GTA'TAC
Cuts at: 2418 2418
Size: 6559
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5820
Fragments arranged by size:
5820 683 56
BtsI GCAGTG_nn'
Cuts at: 4075 4869 4889 4075
Size: 794 20 5745
Fragments arranged by size:
5745 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6524
DraI TTT'AAA
Cuts at: 2584 3004 3863 2584
Size: 420 859 5280
Fragments arranged by size:
5280 859 420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5566 809
Size: 507 4250 1802
Fragments arranged by size:
4250 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3732 4006 4895
Size: 54 1425 50 275 18 1902 274 889
Cuts at: 4895 6524 8
Size: 1629 43
Fragments arranged by size:
1902 1629 1425 889 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4006 1537
Size: 275 2194 4090
Fragments arranged by size:
4090 2194 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 3321 4489 1353
Size: 738 1230 1168 3423
Fragments arranged by size:
3423 1230 1168 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5146 5520 5666 387
Size: 1064 138 3557 374 146 1280
Fragments arranged by size:
3557 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 3771 2476
Size: 1295 5264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 2702 3177 3293 3474 4622 5219
Size: 393 848 222 475 116 181 1148 597
Cuts at: 5219 6006 6274 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1148 848 787 597 475 393 268
222 181 116
EcoO109I rG'GnC_Cy
Cuts at: 2516 2517 2516
Size: 1 6558
EcoRI G'AATT_C
Cuts at: 3868 4246 3868
Size: 378 6181
EcoRV GAT'ATC
Cuts at: 3430 3709 3430
Size: 279 6280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2519 3562 3727 2519
Size: 1043 165 5351
Fragments arranged by size:
5351 1043 165
FspI TGC'GCA
Cuts at: 5065 5065
Size: 6559
HaeII r_GCGC'y
Cuts at: 1678 5706 1678
Size: 4028 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 3167 3199 3350
Size: 32 135 32 484 32 1079 32 151
Cuts at: 3350 3382 5200 5232 5274 5306 1373
Size: 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 1079 484 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2257 2742 3903 4149 669
Size: 988 281 319 485 1161 246 3079
Fragments arranged by size:
3079 1161 988 485 319 281 246
HindIII A'AGCT_T
Cuts at: 748 3189 748
Size: 2441 4118
HpaI GTT'AAC
Cuts at: 2257 4149 2257
Size: 1892 4667
KpnI G_GTAC'C
Cuts at: 3884 3884
Size: 6559
MfeI C'AATT_G
Cuts at: 4158 4158
Size: 6559
MluI A'CGCG_T
Cuts at: 6435 6435
Size: 6559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2183 5673 5857 1305
Size: 878 3490 184 2007
Fragments arranged by size:
3490 2007 878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3734 10
Size: 54 1425 343 1902 2835
Fragments arranged by size:
2835 1902 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2715 4517 4876 5035 518
Size: 648 1549 1802 359 159 2042
Fragments arranged by size:
2042 1802 1549 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 3130 3197 3975 4652 5800 6045 1606
Size: 529 995 67 778 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 778 677 529 245 67
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6007
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6559
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6559
NotI GC'GGCC_GC
Cuts at: 4006 4006
Size: 6559
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 6559
NspI r_CATG'y
Cuts at: 1132 1229 3917 5462 1132
Size: 97 2688 1545 2229
Fragments arranged by size:
2688 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6559
PciI A'CATG_T
Cuts at: 5458 5458
Size: 6559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3780 1108
Size: 2672 3887
PmeI GTTT'AAAC
Cuts at: 3863 3863
Size: 6559
PmlI CAC'GTG
Cuts at: 3748 3748
Size: 6559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 6141 6173 4628
Size: 32 1481 32 5014
Fragments arranged by size:
5014 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6559
PsiI TTA'TAA
Cuts at: 4129 4129
Size: 6559
PspOMI G'GGCC_C
Cuts at: 2516 2516
Size: 6559
PstI C_TGCA'G
Cuts at: 830 3234 3644 3911 830
Size: 2404 410 267 3478
Fragments arranged by size:
3478 2404 410 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6559
PvuII CAG'CTG
Cuts at: 2135 3197 2135
Size: 1062 5497
SacI G_AGCT'C
Cuts at: 2478 3773 2478
Size: 1295 5264
SalI G'TCGA_C
Cuts at: 1936 3901 1936
Size: 1965 4594
SapI GCTCTTCn'nnn_
Cuts at: 3321 3321
Size: 6559
SbfI CC_TGCA'GG
Cuts at: 3911 3911
Size: 6559
ScaI AGT'ACT
Cuts at: 4807 6519 4807
Size: 1712 4847
SfcI C'TryA_G
Cuts at: 826 1045 2511 3230 3640 3907 5042 5723 5914
Size: 219 1466 719 410 267 1135 681 191
Cuts at: 5914 826
Size: 1471
Fragments arranged by size:
1471 1466 1135 719 681 410 267 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6559
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6559
SmaI CCC'GGG
Cuts at: 1872 2111 3886 1872
Size: 239 1775 4545
Fragments arranged by size:
4545 1775 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 3186 3764 4663 5564 5826
Size: 197 926 730 513 578 899 901 262
Cuts at: 5826 820
Size: 1553
Fragments arranged by size:
1553 926 901 899 730 578 513 262
197
SnaBI TAC'GTA
Cuts at: 493 4350 493
Size: 3857 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6559
SphI G_CATG'C
Cuts at: 1132 3917 1132
Size: 2785 3774
SspI AAT'ATT
Cuts at: 5 52 2346 2861 3143 3168 4483 5
Size: 47 2294 515 282 25 1315 2081
Fragments arranged by size:
2294 2081 1315 515 282 47 25
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6559
StyI C'CwwG_G
Cuts at: 513 1065 3023 3998 513
Size: 552 1958 975 3074
Fragments arranged by size:
3074 1958 975 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4586 4603 4756 4941 5386 325
Size: 1255 3006 17 153 185 445 1498
Fragments arranged by size:
3006 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 3124 3834
Size: 274 80 33 51 1443 546 601 710
Cuts at: 3834 4805 6517 96
Size: 971 1712 138
Fragments arranged by size:
1712 1443 971 710 601 546 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 6519 6551 2763
Size: 32 3724 32 2771
Fragments arranged by size:
3724 2771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6559
XbaI T'CTAG_A
Cuts at: 3895 3895
Size: 6559
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6559
XmaI C'CCGG_G
Cuts at: 1870 2109 3884 1870
Size: 239 1775 4545
Fragments arranged by size:
4545 1775 239
XmnI GAAnn'nnTTC
Cuts at: 2088 2100 2205 4688 2088
Size: 12 105 2483 3959
Fragments arranged by size:
3959 2483 105 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4367 276
Size: 53 83 186 936 2833 2468
Fragments arranged by size:
2833 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI