Restriction map for
pFN21AE0323
(Circular) (Six-base) MAPSORT of: pFN21AE0323.seq Check: 8031 from: 1 to: 7237
pFN21AE0323 7237 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4343 4343
Size: 7237
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5047 278
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3111 4580 1937
Size: 1174 1469 4594
Fragments arranged by size:
4594 1469 1174
Acc65I G'GTAC_C
Cuts at: 4558 4558
Size: 7237
AclI AA'CG_TT
Cuts at: 1563 5364 5737 1563
Size: 3801 373 3063
Fragments arranged by size:
3801 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 2327 2597 3325 4332 5300 6684 6952
Size: 524 171 270 728 1007 968 1384 268
Cuts at: 6952 1632
Size: 1917
Fragments arranged by size:
1917 1384 1007 968 728 524 270 268
171
AfeI AGC'GCT
Cuts at: 2702 2702
Size: 7237
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7040
AflIII A'CryG_T
Cuts at: 1203 6136 7113 1203
Size: 4933 977 1327
Fragments arranged by size:
4933 1327 977
AgeI A'CCGG_T
Cuts at: 6068 6068
Size: 7237
AhdI GACnn_n'nnGTC
Cuts at: 3118 3904 5966 3118
Size: 786 2062 4389
Fragments arranged by size:
4389 2062 786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2638 3980 1184
Size: 1454 1342 4441
Fragments arranged by size:
4441 1454 1342
AlwNI CAG_nnn'CTG
Cuts at: 2633 4355 4980 6552 2633
Size: 1722 625 1572 3318
Fragments arranged by size:
3318 1722 1572 625
ApaI G_GGCC'C
Cuts at: 3316 3316
Size: 7237
ApaLI G'TGCA_C
Cuts at: 5294 6450 5294
Size: 1156 6081
ApoI r'AATT_y
Cuts at: 1493 4546 4772 4924 7033 1493
Size: 3053 226 152 2109 1697
Fragments arranged by size:
3053 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5791 160
Size: 872 4759 1606
Fragments arranged by size:
4759 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
AvaI C'yCGr_G
Cuts at: 1870 1943 3424 3486 3624 4562 1870
Size: 73 1481 62 138 938 4545
Fragments arranged by size:
4545 1481 938 138 73 62
AvrII C'CTAG_G
Cuts at: 4250 4375 4250
Size: 125 7112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2141 2174 2141
Size: 33 7204
BamHI G'GATC_C
Cuts at: 3987 4567 4934 3987
Size: 580 367 6290
Fragments arranged by size:
6290 580 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2559 3683 4558 6013
Size: 325 217 627 682 90 1124 875 1455
Cuts at: 6013 618
Size: 1842
Fragments arranged by size:
1842 1455 1124 875 682 627 325 217
90
BanII G_rGCy'C
Cuts at: 1391 2508 3054 3204 3248 3316 3362 3632 4556
Size: 1117 546 150 44 68 46 270 924
Cuts at: 4556 1391
Size: 4072
Fragments arranged by size:
4072 1117 924 546 270 150 68 46
44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 2728 2952 3420 928
Size: 971 143 686 224 468 4745
Fragments arranged by size:
4745 971 686 468 224 143
BbvCI CC'TCA_GC
Cuts at: 2634 3260 3728 2634
Size: 626 468 6143
Fragments arranged by size:
6143 626 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3605 3639 5428 5462 1903
Size: 34 1668 34 1789 34 3678
Fragments arranged by size:
3678 1789 1668 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5129 6339 5129
Size: 1210 6027
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7237
BfrBI ATG'CAT
Cuts at: 3922 3922
Size: 7237
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5848 136
Size: 107 122 71 631 605 4176 1525
Fragments arranged by size:
4176 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3437 7232 3437
Size: 3795 3442
BlpI GC'TnA_GC
Cuts at: 3140 3764 3875 4654 3140
Size: 624 111 779 5723
Fragments arranged by size:
5723 779 624 111
Bme1580I G_kGCm'C
Cuts at: 1790 2782 3316 3779 4250 5298 6454 1790
Size: 992 534 463 471 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 992 534 471 463
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 4968 5926
Size: 416 53 83 399 24 946 2605 958
Cuts at: 5926 442
Size: 1753
Fragments arranged by size:
2605 1753 958 946 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4144 4144
Size: 7237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2226 3205 5897 1239
Size: 806 181 979 2692 2579
Fragments arranged by size:
2692 2579 979 806 181
Bpu10I CC'TnA_GC
Cuts at: 2634 3260 3728 2634
Size: 626 468 6143
Fragments arranged by size:
6143 626 468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5362 6227 6525 5362
Size: 865 298 6074
Fragments arranged by size:
6074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 3476 3891 5900 882
Size: 617 1338 639 415 2009 2219
Fragments arranged by size:
2219 2009 1338 639 617 415
BsaAI yAC'GTr
Cuts at: 493 1204 5028 493
Size: 711 3824 2702
Fragments arranged by size:
3824 2702 711
BsaBI GATnn'nnATC
Cuts at: 7231 7231
Size: 7237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3605 5044 5426 6061
Size: 53 83 186 936 2072 1439 382 635
Cuts at: 6061 275
Size: 1451
Fragments arranged by size:
2072 1451 1439 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3801 5670 6068 6342 6489 6900 3801
Size: 1869 398 274 147 411 4138
Fragments arranged by size:
4138 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7012
Fragments arranged by size:
7012 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2956 3062 3202 3248 3530 4330 4333 1186
Size: 1770 106 140 46 282 800 3 4090
Fragments arranged by size:
4090 1770 800 282 140 106 46 3
BseYI C'CCAG_C
Cuts at: 2689 2939 3161 3308 3315 3837 4296 4392 6440
Size: 250 222 147 7 522 459 96 2048
Cuts at: 6440 6936 2689
Size: 496 2990
Fragments arranged by size:
2990 2048 522 496 459 250 222 147
96 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4418 1163
Size: 573 2682 3982
Fragments arranged by size:
3982 2682 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4687 5448 6476 1540
Size: 275 186 2686 761 1028 2301
Fragments arranged by size:
2686 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2863 3362 3702 5298 5383 6454 2508
Size: 355 499 340 1596 85 1071 3291
Fragments arranged by size:
3291 1596 1071 499 355 340 85
BsiWI C'GTAC_G
Cuts at: 2485 2485
Size: 7237
BsmI GAATG_Cn'
Cuts at: 2627 3410 4285 4748 4841 2627
Size: 783 875 463 93 5023
Fragments arranged by size:
5023 875 783 463 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2782 2863 3054 3204 3248 3316
Size: 399 718 274 81 191 150 44 68
Cuts at: 3316 3362 3632 3702 3779 4250 4556 5298 5383
Size: 46 270 70 77 471 306 742 85
Cuts at: 5383 6454 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 718 471 399 306 274
270 191 150 85 81 77 70 68
46 44
BspEI T'CCGG_A
Cuts at: 3801 6900 3801
Size: 3099 4138
BspHI T'CATG_A
Cuts at: 2709 5126 6856 2709
Size: 2417 1730 3090
Fragments arranged by size:
3090 2417 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3909 4343 4592 844
Size: 3065 434 249 3489
Fragments arranged by size:
3489 3065 434 249
BsrBI CCG'CTC
Cuts at: 1394 1823 5124 1394
Size: 429 3301 3507
Fragments arranged by size:
3507 3301 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2506 5732 5906 65
Size: 1183 1258 3226 174 1396
Fragments arranged by size:
3226 1396 1258 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3093 5881 6068 1700
Size: 110 1283 2788 187 2869
Fragments arranged by size:
2869 2788 1283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7237
BssHII G'CGCG_C
Cuts at: 1925 4400 1925
Size: 2475 4762
BssSI C'ACGA_G
Cuts at: 5297 6309 5297
Size: 1012 6225
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2496 794
Size: 1702 5535
BstBI TT'CG_AA
Cuts at: 6041 6041
Size: 7237
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7237
BstXI CCAn_nnnn'nTGG
Cuts at: 2417 2417
Size: 7237
BstYI r'GATC_y
Cuts at: 1972 2515 3437 3987 4567 4934 5319 5336 6788
Size: 543 922 550 580 367 385 17 1452
Cuts at: 6788 6874 6886 7232 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 922 580 550 543 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2347 2455 3633 3910 513
Size: 552 939 343 108 1178 277 3840
Fragments arranged by size:
3840 1178 939 552 343 277 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2413 2613 4437 487
Size: 683 56 1187 200 1824 3287
Fragments arranged by size:
3287 1824 1187 683 200 56
BtsI GCAGTG_nn'
Cuts at: 2737 2863 2941 3019 3122 3560 4753 5547 5567
Size: 126 78 78 103 438 1193 794 20
Cuts at: 5567 2737
Size: 4407
Fragments arranged by size:
4407 1193 794 438 126 103 78 78
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2444 2479 529
Size: 35 1880 35 5287
Fragments arranged by size:
5287 1880 35 35
DraI TTT'AAA
Cuts at: 4541 4541
Size: 7237
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6244 809
Size: 507 4928 1802
Fragments arranged by size:
4928 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 2695 2821
Size: 54 1425 50 275 18 622 243 126
Cuts at: 2821 2888 3369 4107 4240 4684 5573 7202 8
Size: 67 481 738 133 444 889 1629 43
Fragments arranged by size:
1629 1425 889 738 622 481 444 275
243 133 126 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4684 1537
Size: 275 2872 4090
Fragments arranged by size:
4090 2872 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2172 2286 2291 3192 3516 3522 4012
Size: 750 69 114 5 901 324 6 490
Cuts at: 4012 4351 4508 5167 1353
Size: 339 157 659 3423
Fragments arranged by size:
3423 901 750 659 490 339 324 157
114 69 6 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2965 5824 6198 6344 387
Size: 1064 138 1376 2859 374 146 1280
Fragments arranged by size:
2859 1376 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2506 3360 2506
Size: 854 6383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2156 2226 2327 2597 3205 3325
Size: 393 413 111 70 101 270 608 120
Cuts at: 3325 4332 5300 5897 6684 6952 1239
Size: 1007 968 597 787 268 1524
Fragments arranged by size:
1524 1007 968 787 608 597 413 393
270 268 120 111 101 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2285
Size: 7237
EcoO109I rG'GnC_Cy
Cuts at: 2122 3175 3312 4146 4221 4231 4301 2122
Size: 1053 137 834 75 10 70 5058
Fragments arranged by size:
5058 1053 834 137 75 70 10
EcoRI G'AATT_C
Cuts at: 4546 4924 4546
Size: 378 6859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4041 4041
Size: 7237
FspI TGC'GCA
Cuts at: 5743 5743
Size: 7237
HaeII r_GCGC'y
Cuts at: 1678 2704 3694 6384 1678
Size: 1026 990 2690 2531
Fragments arranged by size:
2690 2531 1026 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2397 2429 3104
Size: 32 135 32 503 32 290 32 675
Cuts at: 3104 3136 3480 3512 3553 3585 3689 3721 3890
Size: 32 344 32 41 32 104 32 169
Cuts at: 3890 3922 4371 4403 5878 5910 5952 5984 1373
Size: 32 449 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 675 503 449 344 290 169
135 104 42 41 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 4581 4827 669
Size: 988 281 259 2384 246 3079
Fragments arranged by size:
3079 2384 988 281 259 246
HindIII A'AGCT_T
Cuts at: 748 2309 748
Size: 1561 5676
HpaI GTT'AAC
Cuts at: 4827 4827
Size: 7237
KpnI G_GTAC'C
Cuts at: 4562 4562
Size: 7237
MfeI C'AATT_G
Cuts at: 4836 4836
Size: 7237
MluI A'CGCG_T
Cuts at: 7113 7113
Size: 7237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3539 4075 4113 4242 6351 6535 1305
Size: 2234 536 38 129 2109 184 2007
Fragments arranged by size:
2234 2109 2007 536 184 129 38
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2454 3371 4109 10
Size: 54 1425 343 622 917 738 3138
Fragments arranged by size:
3138 1425 917 738 622 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 2432 2832 5195 5554 5713 518
Size: 648 1090 176 400 2363 359 159 2042
Fragments arranged by size:
2363 2042 1090 648 400 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2242 2349 3076 4192 4296 4653 5330
Size: 417 219 107 727 1116 104 357 677
Cuts at: 5330 6478 6723 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1116 727 677 417 357 245
219 107 104
NcoI C'CATG_G
Cuts at: 513 1065 2004 3633 513
Size: 552 939 1629 4117
Fragments arranged by size:
4117 1629 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7237
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7237
NotI GC'GGCC_GC
Cuts at: 4684 4684
Size: 7237
NsiI A_TGCA'T
Cuts at: 3924 3924
Size: 7237
NspI r_CATG'y
Cuts at: 1132 1229 2837 3139 3386 4595 6140 1132
Size: 97 1608 302 247 1209 1545 2229
Fragments arranged by size:
2229 1608 1545 1209 302 247 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7237
PciI A'CATG_T
Cuts at: 6136 6136
Size: 7237
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3853 1108
Size: 2745 4492
PfoI T'CCnGG_A
Cuts at: 2203 3292 4200 4223 2203
Size: 1089 908 23 5217
Fragments arranged by size:
5217 1089 908 23
PmeI GTTT'AAAC
Cuts at: 4541 4541
Size: 7237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 5306 5338 6819 6851 2063
Size: 32 3211 32 1481 32 2449
Fragments arranged by size:
3211 2449 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 3175 4221 4231 4301 2122
Size: 1053 1046 10 70 5058
Fragments arranged by size:
5058 1053 1046 70 10
PshAI GACnn'nnGTC
Cuts at: 1613 2802 1613
Size: 1189 6048
PsiI TTA'TAA
Cuts at: 4807 4807
Size: 7237
PspOMI G'GGCC_C
Cuts at: 3312 3312
Size: 7237
PstI C_TGCA'G
Cuts at: 830 2936 3129 3255 3906 4589 830
Size: 2106 193 126 651 683 3478
Fragments arranged by size:
3478 2106 683 651 193 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7237
PvuII CAG'CTG
Cuts at: 2242 3076 2242
Size: 834 6403
SacI G_AGCT'C
Cuts at: 2508 3362 2508
Size: 854 6383
SacII CC_GC'GG
Cuts at: 2350 2350
Size: 7237
SalI G'TCGA_C
Cuts at: 1936 4579 1936
Size: 2643 4594
SanDI GG'GwC_CC
Cuts at: 2122 3175 4221 4301 2122
Size: 1053 1046 80 5058
Fragments arranged by size:
5058 1053 1046 80
SapI GCTCTTCn'nnn_
Cuts at: 3192 4012 4508 3192
Size: 820 496 5921
Fragments arranged by size:
5921 820 496
SbfI CC_TGCA'GG
Cuts at: 4589 4589
Size: 7237
ScaI AGT'ACT
Cuts at: 2159 5485 7197 2159
Size: 3326 1712 2199
Fragments arranged by size:
3326 2199 1712
SfcI C'TryA_G
Cuts at: 826 1045 2161 2932 3125 3251 3286 3451 3892
Size: 219 1116 771 193 126 35 165 441
Cuts at: 3892 3902 4585 5720 6401 6592 826
Size: 10 683 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 771 683 681 441 219
193 191 165 126 35 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7237
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7237
SmaI CCC'GGG
Cuts at: 1872 3426 4564 1872
Size: 1554 1138 4545
Fragments arranged by size:
4545 1554 1138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5341 6242 6504 820
Size: 197 926 3398 901 262 1553
Fragments arranged by size:
3398 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5028 493
Size: 4535 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7237
SphI G_CATG'C
Cuts at: 1132 2837 4595 1132
Size: 1705 1758 3774
Fragments arranged by size:
3774 1758 1705
SspI AAT'ATT
Cuts at: 5 52 2765 5161 5
Size: 47 2713 2396 2081
Fragments arranged by size:
2713 2396 2081 47
StuI AGG'CCT
Cuts at: 1517 2362 3666 1517
Size: 845 1304 5088
Fragments arranged by size:
5088 1304 845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2800 2997 3633 4250 4375 4676
Size: 552 939 796 197 636 617 125 301
Cuts at: 4676 513
Size: 3074
Fragments arranged by size:
3074 939 796 636 617 552 301 197
125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3175 5264 5281 5434 5619 6064 325
Size: 1255 1595 2089 17 153 185 445 1498
Fragments arranged by size:
2089 1595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 5483 7195
Size: 274 80 33 51 1443 180 3326 1712
Cuts at: 7195 96
Size: 138
Fragments arranged by size:
3326 1712 1443 274 180 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3735 3767 7197 7229 3735
Size: 32 3430 32 3743
Fragments arranged by size:
3743 3430 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4258 1316
Size: 2942 4295
XbaI T'CTAG_A
Cuts at: 4573 4573
Size: 7237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2417 3640 2417
Size: 1223 6014
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7237
XmaI C'CCGG_G
Cuts at: 1870 3424 4562 1870
Size: 1554 1138 4545
Fragments arranged by size:
4545 1554 1138
XmnI GAAnn'nnTTC
Cuts at: 2312 5366 2312
Size: 3054 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5045 276
Size: 53 83 186 936 3511 2468
Fragments arranged by size:
3511 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SexAI SfiI SfoI SrfI
SwaI