Restriction map for
pFN21AE0322
(Circular) (Six-base) MAPSORT of: pFN21AE0322.seq Check: 2056 from: 1 to: 6445
pFN21AE0322 6445 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 3788 1937
Size: 1098 753 4594
Fragments arranged by size:
4594 1098 753
Acc65I G'GTAC_C
Cuts at: 2088 3766 2088
Size: 1678 4767
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3343 3440 4508 5892 6160 1632
Size: 1711 97 1068 1384 268 1917
Fragments arranged by size:
1917 1711 1384 1068 268 97
AfeI AGC'GCT
Cuts at: 2626 2626
Size: 6445
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 2931
Size: 32 6413
AlwNI CAG_nnn'CTG
Cuts at: 3379 4188 5760 3379
Size: 809 1572 4064
Fragments arranged by size:
4064 1572 809
ApaI G_GGCC'C
Cuts at: 2390 2390
Size: 6445
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 3754 3980 4132 6241 1493
Size: 2261 226 152 2109 1697
Fragments arranged by size:
2261 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2194 2383 2894 3458 3770 1870
Size: 73 251 189 511 564 312 4545
Fragments arranged by size:
4545 564 511 312 251 189 73
BamHI G'GATC_C
Cuts at: 2077 2329 3775 4142 2077
Size: 252 1446 367 4380
Fragments arranged by size:
4380 1446 367 252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2180 2291 2545 3766
Size: 325 217 627 301 92 111 254 1221
Cuts at: 3766 5221 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1221 627 325 301 254 217
111 92
BanII G_rGCy'C
Cuts at: 1391 2207 2316 2390 2879 3393 3764 1391
Size: 816 109 74 489 514 371 4072
Fragments arranged by size:
4072 816 514 489 371 109 74
BbeI G_GCGC'C
Cuts at: 2295 2295
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2398 2432 4636 4670 1903
Size: 34 461 34 2204 34 3678
Fragments arranged by size:
3678 2204 461 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 3281 3374
Size: 107 122 71 631 605 577 1032 93
Cuts at: 3374 5056 136
Size: 1682 1525
Fragments arranged by size:
1682 1525 1032 631 605 577 122 107
93 71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 3401 3862 3401
Size: 461 5984
Bme1580I G_kGCm'C
Cuts at: 1790 2390 4506 5662 1790
Size: 600 2116 1156 2573
Fragments arranged by size:
2573 2116 1156 600
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 3081 3544
Size: 416 53 83 399 24 591 1073 463
Cuts at: 3544 4176 5134 442
Size: 632 958 1753
Fragments arranged by size:
1753 1073 958 632 591 463 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3537 1056
Size: 2481 3964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 2954 2967 5105 1239
Size: 952 763 13 2138 2579
Fragments arranged by size:
2579 2138 952 763 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4570 5435 5733 2708
Size: 1862 865 298 3420
Fragments arranged by size:
3420 1862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5108 882
Size: 617 3609 2219
Fragments arranged by size:
3609 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4236 493
Size: 711 3032 2702
Fragments arranged by size:
3032 2702 711
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 4252 4634 5269
Size: 53 83 186 936 759 1960 382 635
Cuts at: 5269 275
Size: 1451
Fragments arranged by size:
1960 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2671 4878 5276 5550 5697 6108 2671
Size: 2207 398 274 147 411 3008
Fragments arranged by size:
3008 2207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2578 2608 1178
Size: 30 165 30 597 30 548 30 5015
Fragments arranged by size:
5015 597 548 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 2731 3275 3278 1186
Size: 1040 505 544 3 4353
Fragments arranged by size:
4353 1040 544 505 3
BseYI C'CCAG_C
Cuts at: 3491 5648 6144 3491
Size: 2157 496 3792
Fragments arranged by size:
3792 2157 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5872
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 3895 4656 5684 1540
Size: 275 186 42 1852 761 1028 2301
Fragments arranged by size:
2301 1852 1028 761 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2648 2724 4506 4591 5662 2648
Size: 76 1782 85 1071 3431
Fragments arranged by size:
3431 1782 1071 85 76
BsmI GAATG_Cn'
Cuts at: 2932 3956 4049 2932
Size: 1024 93 5328
Fragments arranged by size:
5328 1024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2207 2316 2390 2648 2724 2879 3393
Size: 399 417 109 74 258 76 155 514
Cuts at: 3393 3764 4506 4591 5662 1391
Size: 371 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 417 399 371 258
155 109 85 76 74
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 3588 4334 6064 3588
Size: 746 1730 3969
Fragments arranged by size:
3969 1730 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2580 3672 3800 844
Size: 1736 1092 128 3489
Fragments arranged by size:
3489 1736 1092 128
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 4332 1394
Size: 429 1723 786 3507
Fragments arranged by size:
3507 1723 786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2294 2685 5089 5276 1700
Size: 110 275 209 391 2404 187 2869
Fragments arranged by size:
2869 2404 391 275 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 2059 3348 1925
Size: 134 1289 5022
Fragments arranged by size:
5022 1289 134
BssSI C'ACGA_G
Cuts at: 3215 4505 5517 3215
Size: 1290 1012 4143
Fragments arranged by size:
4143 1290 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6445
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 2077 2329 3775 4142 4527 4544 5996 6082
Size: 105 252 1446 367 385 17 1452 86
Cuts at: 6082 6094 6440 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1446 385 367 346 252 105
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2398 2502 2398
Size: 104 6341
BtgI C'CryG_G
Cuts at: 513 1065 2004 2377 2871 513
Size: 552 939 373 494 4087
Fragments arranged by size:
4087 939 552 494 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5706
Fragments arranged by size:
5706 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 3961 4755 4775 3607
Size: 354 794 20 5277
Fragments arranged by size:
5277 794 354 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3299 5452 809
Size: 507 1983 2153 1802
Fragments arranged by size:
2153 1983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2241 3649
Size: 54 1425 50 275 18 210 201 1408
Cuts at: 3649 3892 4781 6410 8
Size: 243 889 1629 43
Fragments arranged by size:
1629 1425 1408 889 275 243 210 201
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 3892 1537
Size: 275 228 1852 4090
Fragments arranged by size:
4090 1852 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 3398 4375 1353
Size: 2045 977 3423
Fragments arranged by size:
3423 2045 977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5032 5406 5552 387
Size: 1064 138 3443 374 146 1280
Fragments arranged by size:
3443 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2954 2967 3343 3440 4508 5105
Size: 393 559 763 13 376 97 1068 597
Cuts at: 5105 5892 6160 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1068 787 763 597 559 393 376
268 97 13
EcoO109I rG'GnC_Cy
Cuts at: 2387 2898 2998 3721 2387
Size: 511 100 723 5111
Fragments arranged by size:
5111 723 511 100
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
EcoRV GAT'ATC
Cuts at: 2770 2770
Size: 6445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3067 3218 3067
Size: 151 6294
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 2099 2265 2295 2628 3271 5592 1678
Size: 421 166 30 333 643 2321 2531
Fragments arranged by size:
2531 2321 643 421 333 166 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 5086 5118 5160
Size: 32 135 32 1006 32 2476 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 2476 1006 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 2291 2291
Size: 6445
KpnI G_GTAC'C
Cuts at: 2092 3770 2092
Size: 1678 4767
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5559 5743 1305
Size: 4254 184 2007
Fragments arranged by size:
4254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4623
Fragments arranged by size:
4623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4403 4762 4921 518
Size: 648 3237 359 159 2042
Fragments arranged by size:
3237 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2923 2959 3253 3861 4538 5686 5931
Size: 444 873 36 294 608 677 1148 245
Cuts at: 5931 1606
Size: 2120
Fragments arranged by size:
2120 1148 873 677 608 444 294 245
36
NaeI GCC'GGC
Cuts at: 2296 2687 2296
Size: 391 6054
NarI GG'CG_CC
Cuts at: 2292 2292
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 2004 2871 513
Size: 552 939 867 4087
Fragments arranged by size:
4087 939 867 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NgoMIV G'CCGG_C
Cuts at: 2294 2685 2294
Size: 391 6054
NheI G'CTAG_C
Cuts at: 1052 3533 1052
Size: 2481 3964
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3236 3803 5348 1132
Size: 97 2007 567 1545 2229
Fragments arranged by size:
2229 2007 1545 567 97
PasI CC'CwG_GG
Cuts at: 1348 2318 2492 1348
Size: 970 174 5301
Fragments arranged by size:
5301 970 174
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6445
PfoI T'CCnGG_A
Cuts at: 3335 3507 3335
Size: 172 6273
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 4514 4546 6027 6059 2726
Size: 32 1756 32 1481 32 3112
Fragments arranged by size:
3112 1756 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2898 3721 2898
Size: 823 5622
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 2386 2386
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2363 2586 3429 3797 830
Size: 1533 223 843 368 3478
Fragments arranged by size:
3478 1533 843 368 223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2959 3253 2959
Size: 294 6151
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SapI GCTCTTCn'nnn_
Cuts at: 3398 3398
Size: 6445
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SfcI C'TryA_G
Cuts at: 826 1045 2359 2582 3425 3793 4928 5609 5800
Size: 219 1314 223 843 368 1135 681 191
Cuts at: 5800 826
Size: 1471
Fragments arranged by size:
1471 1314 1135 843 681 368 223 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2249 2249
Size: 6445
SfoI GGC'GCC
Cuts at: 2293 2293
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 2294 1700
Size: 594 5851
SmaI CCC'GGG
Cuts at: 1872 2196 2385 2896 3772 1872
Size: 324 189 511 876 4545
Fragments arranged by size:
4545 876 511 324 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 4549 5450 5712 820
Size: 197 926 780 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 780 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3236 3803 1132
Size: 2104 567 3774
Fragments arranged by size:
3774 2104 567
SrfI GCCC'GGGC
Cuts at: 2196 2385 2196
Size: 189 6256
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6445
StyI C'CwwG_G
Cuts at: 513 1065 2004 2871 2991 3325 3884 513
Size: 552 939 867 120 334 559 3074
Fragments arranged by size:
3074 939 867 559 552 334 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2390 3086 4472 4489 4642 4827
Size: 1255 665 145 696 1386 17 153 185
Cuts at: 4827 5272 325
Size: 445 1498
Fragments arranged by size:
1498 1386 1255 696 665 445 185 153
145 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 2118 4691
Size: 274 80 33 51 1443 54 87 2573
Cuts at: 4691 6403 96
Size: 1712 138
Fragments arranged by size:
2573 1712 1443 274 138 87 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 3092 3124 6405 6437 2000
Size: 32 1060 32 3281 32 2008
Fragments arranged by size:
3281 2008 1060 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2843 3296 1316
Size: 1527 453 4465
Fragments arranged by size:
4465 1527 453
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2998 3094 2419
Size: 579 96 5770
Fragments arranged by size:
5770 579 96
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 2194 2383 2894 3770 1870
Size: 324 189 511 876 4545
Fragments arranged by size:
4545 876 511 324 189
XmnI GAAnn'nnTTC
Cuts at: 4574 4574
Size: 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SexAI SwaI