Restriction map for
pFN21AE0314
(Circular) (Six-base) MAPSORT of: pFN21AE0314.seq Check: 298 from: 1 to: 6853
pFN21AE0314 6853 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3500 3500
Size: 6853
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4663 278
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4196 1937
Size: 2259 4594
Acc65I G'GTAC_C
Cuts at: 4174 4174
Size: 6853
AclI AA'CG_TT
Cuts at: 1563 4980 5353 1563
Size: 3417 373 3063
Fragments arranged by size:
3417 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 2954 4017 4916 6300 6568 1632
Size: 915 407 1063 899 1384 268 1917
Fragments arranged by size:
1917 1384 1063 915 899 407 268
AfeI AGC'GCT
Cuts at: 2808 2808
Size: 6853
AflII C'TTAA_G
Cuts at: 820 1017 3041 820
Size: 197 2024 4632
Fragments arranged by size:
4632 2024 197
AflIII A'CryG_T
Cuts at: 1203 3461 4150 5752 6729 1203
Size: 2258 689 1602 977 1327
Fragments arranged by size:
2258 1602 1327 977 689
AgeI A'CCGG_T
Cuts at: 5684 5684
Size: 6853
AhdI GACnn_n'nnGTC
Cuts at: 5582 5582
Size: 6853
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6853
AlwNI CAG_nnn'CTG
Cuts at: 2486 2867 4596 6168 2486
Size: 381 1729 1572 3171
Fragments arranged by size:
3171 1729 1572 381
ApaLI G'TGCA_C
Cuts at: 4910 6066 4910
Size: 1156 5697
ApoI r'AATT_y
Cuts at: 1493 2846 2981 3417 3448 4162 4388 4540 6649
Size: 1353 135 436 31 714 226 152 2109
Cuts at: 6649 1493
Size: 1697
Fragments arranged by size:
2109 1697 1353 714 436 226 152 135
31
AseI AT'TA_AT
Cuts at: 160 1032 2465 5407 160
Size: 872 1433 2942 1606
Fragments arranged by size:
2942 1606 1433 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
AvaI C'yCGr_G
Cuts at: 1870 1943 3015 3687 4178 1870
Size: 73 1072 672 491 4545
Fragments arranged by size:
4545 1072 672 491 73
AvrII C'CTAG_G
Cuts at: 2561 2561
Size: 6853
BamHI G'GATC_C
Cuts at: 2827 3633 4183 4550 2827
Size: 806 550 367 5130
Fragments arranged by size:
5130 806 550 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2701 3279 4174 5629 618
Size: 325 217 627 914 578 895 1455 1842
Fragments arranged by size:
1842 1455 914 895 627 578 325 217
BanII G_rGCy'C
Cuts at: 1391 2598 3138 3169 4172 1391
Size: 1207 540 31 1003 4072
Fragments arranged by size:
4072 1207 1003 540 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 2493 4005 928
Size: 971 510 84 1512 3776
Fragments arranged by size:
3776 1512 971 510 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5044 5078 1903
Size: 34 3107 34 3678
Fragments arranged by size:
3678 3107 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2568 4745 5955 2568
Size: 2177 1210 3466
Fragments arranged by size:
3466 2177 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6853
BfrBI ATG'CAT
Cuts at: 3386 3398 3386
Size: 12 6841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 5464 136
Size: 107 122 71 631 605 451 3341 1525
Fragments arranged by size:
3341 1525 631 605 451 122 107 71
BglII A'GATC_T
Cuts at: 2426 6848 2426
Size: 4422 2431
BlpI GC'TnA_GC
Cuts at: 4270 4270
Size: 6853
Bme1580I G_kGCm'C
Cuts at: 1790 2706 3284 3552 4914 6070 1790
Size: 916 578 268 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 916 578 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6853
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 4584 5542
Size: 416 53 83 399 24 666 2501 958
Cuts at: 5542 442
Size: 1753
Fragments arranged by size:
2501 1753 958 666 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6853
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 2946
Size: 6853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2026 2033 2094 5513 1239
Size: 787 7 61 3419 2579
Fragments arranged by size:
3419 2579 787 61 7
Bpu10I CC'TnA_GC
Cuts at: 2589 3693 2589
Size: 1104 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3617 3980 4978 5843 6141 3617
Size: 363 998 865 298 4329
Fragments arranged by size:
4329 998 865 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2934 5516 882
Size: 617 1435 2582 2219
Fragments arranged by size:
2582 2219 1435 617
BsaAI yAC'GTr
Cuts at: 493 1204 4644 493
Size: 711 3440 2702
Fragments arranged by size:
3440 2702 711
BsaBI GATnn'nnATC
Cuts at: 2431 6847 2431
Size: 4416 2437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4660 5042 5677 275
Size: 53 83 186 936 3127 382 635 1451
Fragments arranged by size:
3127 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2719 5286 5684 5958 6105 6516 2719
Size: 2567 398 274 147 411 3056
Fragments arranged by size:
3056 2567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 1178
Size: 30 165 30 664 30 5934
Fragments arranged by size:
5934 664 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2171 2297 2512 2546 2777 3183 1186
Size: 982 3 126 215 34 231 406 4856
Fragments arranged by size:
4856 982 406 231 215 126 34 3
BseYI C'CCAG_C
Cuts at: 2177 6056 6552 2177
Size: 3879 496 2478
Fragments arranged by size:
3879 2478 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3528 1163
Size: 573 1792 4488
Fragments arranged by size:
4488 1792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4303 5064 6092 1540
Size: 275 186 2302 761 1028 2301
Fragments arranged by size:
2302 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 3138 4914 4999 6070 2598
Size: 540 1776 85 1071 3381
Fragments arranged by size:
3381 1776 1071 540 85
BsmI GAATG_Cn'
Cuts at: 4364 4457 4364
Size: 93 6760
BsmBI CGTCTCn'nnnn_
Cuts at: 3974 3974
Size: 6853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2598 2706 3138 3169 3284 3552 4172
Size: 399 808 108 432 31 115 268 620
Cuts at: 4172 4914 4999 6070 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 620 432 399 268
115 108 85 31
BspEI T'CCGG_A
Cuts at: 6516 6516
Size: 6853
BspHI T'CATG_A
Cuts at: 4742 6472 4742
Size: 1730 5123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 4208 844
Size: 2656 708 3489
Fragments arranged by size:
3489 2656 708
BsrBI CCG'CTC
Cuts at: 1394 1823 4740 1394
Size: 429 2917 3507
Fragments arranged by size:
3507 2917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5348 5522 65
Size: 1183 4100 174 1396
Fragments arranged by size:
4100 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5497 5684 1700
Size: 110 3687 187 2869
Fragments arranged by size:
3687 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6853
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6853
BssSI C'ACGA_G
Cuts at: 4913 5925 4913
Size: 1012 5841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6853
BstBI TT'CG_AA
Cuts at: 5657 5657
Size: 6853
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6853
BstYI r'GATC_y
Cuts at: 1972 2426 2827 3633 4183 4550 4935 4952 6404
Size: 454 401 806 550 367 385 17 1452
Cuts at: 6404 6490 6502 6848 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 806 550 454 401 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3149 3149
Size: 6853
BtgI C'CryG_G
Cuts at: 513 1065 2004 3552 513
Size: 552 939 1548 3814
Fragments arranged by size:
3814 1548 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6114
Fragments arranged by size:
6114 683 56
BtsI GCAGTG_nn'
Cuts at: 3830 4369 5163 5183 3830
Size: 539 794 20 5500
Fragments arranged by size:
5500 794 539 20
ClaI AT'CG_AT
Cuts at: 1554 3064 1554
Size: 1510 5343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6818
DraI TTT'AAA
Cuts at: 2373 2980 3070 3453 4157 2373
Size: 607 90 383 704 5069
Fragments arranged by size:
5069 704 607 383 90
DraIII CAC_nnn'GTG
Cuts at: 2023 2023
Size: 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5860 809
Size: 507 4544 1802
Fragments arranged by size:
4544 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4300 5189
Size: 54 1425 50 275 18 177 2293 889
Cuts at: 5189 6818 8
Size: 1629 43
Fragments arranged by size:
2293 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4300 1537
Size: 275 2488 4090
Fragments arranged by size:
4090 2488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2298 2425 2523 2817 3126 3615 4122
Size: 792 153 127 98 294 309 489 507
Cuts at: 4122 4783 1353
Size: 661 3423
Fragments arranged by size:
3423 792 661 507 489 309 294 153
127 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5440 5814 5960 387
Size: 1064 138 3851 374 146 1280
Fragments arranged by size:
3851 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 3136 2596
Size: 540 6313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2033 2094 2547 2954 4017 4916
Size: 393 394 7 61 453 407 1063 899
Cuts at: 4916 5513 6300 6568 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1063 899 787 597 453 407 394
393 268 61 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2490 2490
Size: 6853
EcoO109I rG'GnC_Cy
Cuts at: 2104 2116 3301 2104
Size: 12 1185 5656
Fragments arranged by size:
5656 1185 12
EcoRI G'AATT_C
Cuts at: 4162 4540 4162
Size: 378 6475
EcoRV GAT'ATC
Cuts at: 2788 2788
Size: 6853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2426 2972 2426
Size: 546 6307
FspI TGC'GCA
Cuts at: 5359 5359
Size: 6853
HaeII r_GCGC'y
Cuts at: 1678 2810 6000 1678
Size: 1132 3190 2531
Fragments arranged by size:
3190 2531 1132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 5494 5526 5568
Size: 32 135 32 495 32 3395 32 42
Cuts at: 5568 5600 1373
Size: 32 2626
Fragments arranged by size:
3395 2626 495 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4197 4443 669
Size: 988 281 2259 246 3079
Fragments arranged by size:
3079 2259 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4009 748
Size: 3261 3592
HpaI GTT'AAC
Cuts at: 4443 4443
Size: 6853
KpnI G_GTAC'C
Cuts at: 4178 4178
Size: 6853
MfeI C'AATT_G
Cuts at: 4452 4452
Size: 6853
MluI A'CGCG_T
Cuts at: 6729 6729
Size: 6853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3594 5967 6151 1305
Size: 2289 2373 184 2007
Fragments arranged by size:
2373 2289 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4854
Fragments arranged by size:
4854 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2277 4811 5170 5329 518
Size: 648 1111 2534 359 159 2042
Fragments arranged by size:
2534 2042 1111 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2129 2177 2669 3028 4269 4946 6094 6339
Size: 523 48 492 359 1241 677 1148 245
Cuts at: 6339 1606
Size: 2120
Fragments arranged by size:
2120 1241 1148 677 523 492 359 245
48
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5362
Fragments arranged by size:
5362 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6853
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6853
NotI GC'GGCC_GC
Cuts at: 4300 4300
Size: 6853
NsiI A_TGCA'T
Cuts at: 3388 3400 3388
Size: 12 6841
NspI r_CATG'y
Cuts at: 1132 1229 2228 3398 4211 5756 1132
Size: 97 999 1170 813 1545 2229
Fragments arranged by size:
2229 1545 1170 999 813 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6853
PciI A'CATG_T
Cuts at: 5752 5752
Size: 6853
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 1108
Size: 915 5938
PmeI GTTT'AAAC
Cuts at: 4157 4157
Size: 6853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4922 4954 6435 6467 4922
Size: 32 1481 32 5308
Fragments arranged by size:
5308 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 3301 2104
Size: 1197 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6853
PsiI TTA'TAA
Cuts at: 4423 4423
Size: 6853
PstI C_TGCA'G
Cuts at: 830 4205 830
Size: 3375 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6853
PvuII CAG'CTG
Cuts at: 2669 3028 2669
Size: 359 6494
SacI G_AGCT'C
Cuts at: 2598 3138 2598
Size: 540 6313
SalI G'TCGA_C
Cuts at: 1936 4195 1936
Size: 2259 4594
SapI GCTCTTCn'nnn_
Cuts at: 3126 3126
Size: 6853
SbfI CC_TGCA'GG
Cuts at: 4205 4205
Size: 6853
ScaI AGT'ACT
Cuts at: 3957 3965 5101 6813 3957
Size: 8 1136 1712 3997
Fragments arranged by size:
3997 1712 1136 8
SfcI C'TryA_G
Cuts at: 826 1045 4201 5336 6017 6208 826
Size: 219 3156 1135 681 191 1471
Fragments arranged by size:
3156 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6853
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6853
SmaI CCC'GGG
Cuts at: 1872 3017 3689 4180 1872
Size: 1145 672 491 4545
Fragments arranged by size:
4545 1145 672 491
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3041 3596 3959 4957 5858 6120
Size: 197 926 1098 555 363 998 901 262
Cuts at: 6120 820
Size: 1553
Fragments arranged by size:
1553 1098 998 926 901 555 363 262
197
SnaBI TAC'GTA
Cuts at: 493 4644 493
Size: 4151 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6853
SphI G_CATG'C
Cuts at: 1132 4211 1132
Size: 3079 3774
SspI AAT'ATT
Cuts at: 5 52 4777 5
Size: 47 4725 2081
Fragments arranged by size:
4725 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6853
StyI C'CwwG_G
Cuts at: 513 1065 2004 2561 3482 4292 513
Size: 552 939 557 921 810 3074
Fragments arranged by size:
3074 939 921 810 557 552
SwaI ATTT'AAAT
Cuts at: 2980 2980
Size: 6853
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2689 4880 4897 5050 5235 5680 325
Size: 1255 1109 2191 17 153 185 445 1498
Fragments arranged by size:
2191 1498 1255 1109 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3955 3963 5099
Size: 274 80 33 51 1443 1978 8 1136
Cuts at: 5099 6811 96
Size: 1712 138
Fragments arranged by size:
1978 1712 1443 1136 274 138 80 51
33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6813 6845 6813
Size: 32 6821
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6853
XbaI T'CTAG_A
Cuts at: 4189 4189
Size: 6853
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6853
XmaI C'CCGG_G
Cuts at: 1870 3015 3687 4178 1870
Size: 1145 672 491 4545
Fragments arranged by size:
4545 1145 672 491
XmnI GAAnn'nnTTC
Cuts at: 2846 4982 2846
Size: 2136 4717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4661 276
Size: 53 83 186 936 3127 2468
Fragments arranged by size:
3127 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BsiWI BstXI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI XcmI