Restriction map for
pFN21AE0312
(Circular) (Six-base) MAPSORT of: pFN21AE0312.seq Check: 960 from: 1 to: 6280
pFN21AE0312 6280 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2315 3288 2315
Size: 973 5307
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 3623 1937
Size: 822 864 4594
Fragments arranged by size:
4594 864 822
Acc65I G'GTAC_C
Cuts at: 3366 3601 3366
Size: 235 6045
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 3422 3540 4343 5727 5995 1632
Size: 926 864 118 803 1384 268 1917
Fragments arranged by size:
1917 1384 926 864 803 268 118
AfeI AGC'GCT
Cuts at: 2919 2919
Size: 6280
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3153 3185 3153
Size: 32 6248
AlwNI CAG_nnn'CTG
Cuts at: 2222 2735 2820 3289 4023 5595 2222
Size: 513 85 469 734 1572 2907
Fragments arranged by size:
2907 1572 734 513 469 85
ApaI G_GGCC'C
Cuts at: 2173 2173
Size: 6280
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 2368 3135 3605 1870
Size: 73 334 91 767 470 4545
Fragments arranged by size:
4545 767 470 334 91 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3215 3310 3366 3601 5056
Size: 325 217 627 1428 95 56 235 1455
Cuts at: 5056 618
Size: 1842
Fragments arranged by size:
1842 1455 1428 627 325 235 217 95
56
BanII G_rGCy'C
Cuts at: 1391 2173 2465 2985 3432 3599 1391
Size: 782 292 520 447 167 4072
Fragments arranged by size:
4072 782 520 447 292 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5309
BbvCI CC'TCA_GC
Cuts at: 2223 3276 2223
Size: 1053 5227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4471 4505 1903
Size: 34 2534 34 3678
Fragments arranged by size:
3678 2534 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2072 4172 5382 2072
Size: 2100 1210 2970
Fragments arranged by size:
2970 2100 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3213 3394 4891
Size: 107 122 71 631 605 1541 181 1497
Cuts at: 4891 136
Size: 1525
Fragments arranged by size:
1541 1525 1497 631 605 181 122 107
71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 3697 3697
Size: 6280
Bme1580I G_kGCm'C
Cuts at: 1790 2173 3218 4341 5497 1790
Size: 383 1045 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 1045 383
BmgBI CAC'GTC
Cuts at: 1318 2932 1318
Size: 1614 4666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3025 3091 4011
Size: 416 53 83 399 24 1608 66 920
Cuts at: 4011 4969 442
Size: 958 1753
Fragments arranged by size:
1753 1608 958 920 416 399 83 66
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 4940 1239
Size: 1412 2289 2579
Fragments arranged by size:
2579 2289 1412
Bpu10I CC'TnA_GC
Cuts at: 2163 2223 3276 3304 2163
Size: 60 1053 28 5139
Fragments arranged by size:
5139 1053 60 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2325 2969 4405 5270 5568 2325
Size: 644 1436 865 298 3037
Fragments arranged by size:
3037 1436 865 644 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4087 4469 5104 275
Size: 53 83 186 936 2554 382 635 1451
Fragments arranged by size:
2554 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4713 5111 5385 5532 5943 4713
Size: 398 274 147 411 5050
Fragments arranged by size:
5050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3153 3183 1178
Size: 30 165 30 1750 30 4275
Fragments arranged by size:
4275 1750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2045 2984 2987 1186
Size: 856 3 939 3 4479
Fragments arranged by size:
4479 939 856 3 3
BseYI C'CCAG_C
Cuts at: 2215 2655 3270 5483 5979 2215
Size: 440 615 2213 496 2516
Fragments arranged by size:
2516 2213 615 496 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5707
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2985 3305 4341 4426 5497 2985
Size: 320 1036 85 1071 3768
Fragments arranged by size:
3768 1071 1036 320 85
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2465 2985 3218 3305 3432 3599
Size: 399 383 292 520 233 87 127 167
Cuts at: 3599 4341 4426 5497 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 383 292 233
167 127 87 85
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 2449 3288 3635 844
Size: 1471 134 839 347 3489
Fragments arranged by size:
3489 1471 839 347 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 2167 2186 2947 3262 4167 1394
Size: 429 235 109 19 761 315 905 3507
Fragments arranged by size:
3507 905 761 429 315 235 109 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 3569 4775 4949 65
Size: 1183 1462 859 1206 174 1396
Fragments arranged by size:
1462 1396 1206 1183 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 2376 4924 5111 1700
Size: 110 341 225 2548 187 2869
Fragments arranged by size:
2869 2548 341 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2310 794
Size: 1516 4764
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 3610 3977 4362 4379 5831 5917 5929 6275
Size: 1638 367 385 17 1452 86 12 346
Cuts at: 6275 1972
Size: 1977
Fragments arranged by size:
1977 1638 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2760 2760
Size: 6280
Bsu36I CC'TnA_GG
Cuts at: 2832 3315 2832
Size: 483 5797
BtgI C'CryG_G
Cuts at: 513 1065 2004 3370 513
Size: 552 939 1366 3423
Fragments arranged by size:
3423 1366 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 3479 3796 4590 4610 3479
Size: 317 794 20 5149
Fragments arranged by size:
5149 794 317 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 3334 3727
Size: 54 1425 50 275 18 420 1084 393
Cuts at: 3727 4616 6245 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1084 889 420 393 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2742 2847 2856 3398 4210 1353
Size: 759 630 105 9 542 812 3423
Fragments arranged by size:
3423 812 759 630 542 105 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 3262 3476 4867 5241 5387
Size: 1064 138 450 1223 214 1391 374 146
Cuts at: 5387 387
Size: 1280
Fragments arranged by size:
1391 1280 1223 1064 450 374 214 146
138
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 6280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2558 2651 3422 3540 4343 4940 5727
Size: 393 926 93 771 118 803 597 787
Cuts at: 5727 5995 1239
Size: 268 1524
Fragments arranged by size:
1524 926 803 787 771 597 393 268
118 93
EcoNI CCTnn'n_nnAGG
Cuts at: 3535 3535
Size: 6280
EcoO109I rG'GnC_Cy
Cuts at: 2170 3286 3319 3408 2170
Size: 1116 33 89 5042
Fragments arranged by size:
5042 1116 89 33
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 2012 2698 2921 3229 5427 1678
Size: 334 686 223 308 2198 2531
Fragments arranged by size:
2531 2198 686 334 308 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3129 3161 3551 3583 4921
Size: 32 135 32 1557 32 390 32 1338
Cuts at: 4921 4953 4995 5027 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1557 1338 390 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3624 3870 669
Size: 988 281 1686 246 3079
Fragments arranged by size:
3079 1686 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 3370 3605 3370
Size: 235 6045
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 5394 5578 1305
Size: 1175 2914 184 2007
Fragments arranged by size:
2914 2007 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3336 10
Size: 54 1425 343 1504 2954
Fragments arranged by size:
2954 1504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2874 3098 3506 4238 4597 4756 518
Size: 648 1708 224 408 732 359 159 2042
Fragments arranged by size:
2042 1708 732 648 408 359 224 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2659 3696 4373 5521 5766 1606
Size: 616 437 1037 677 1148 245 2120
Fragments arranged by size:
2120 1148 1037 677 616 437 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3370 513
Size: 552 939 1366 3423
Fragments arranged by size:
3423 1366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2365 3638 5183 1132
Size: 97 1136 1273 1545 2229
Fragments arranged by size:
2229 1545 1273 1136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6280
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3289 1108
Size: 2181 4099
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3153 3185 4349 4381 5862 5894 3153
Size: 32 1164 32 1481 32 3539
Fragments arranged by size:
3539 1481 1164 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PspOMI G'GGCC_C
Cuts at: 2169 2169
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2496 2664 3299 3632 830
Size: 1666 168 635 333 3478
Fragments arranged by size:
3478 1666 635 333 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2659 2659
Size: 6280
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SapI GCTCTTCn'nnn_
Cuts at: 2112 2742 2112
Size: 630 5650
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SexAI A'CCwGG_T
Cuts at: 2046 2046
Size: 6280
SfcI C'TryA_G
Cuts at: 826 1045 2492 2660 3295 3383 3628 4763 5444
Size: 219 1447 168 635 88 245 1135 681
Cuts at: 5444 5635 826
Size: 191 1471
Fragments arranged by size:
1471 1447 1135 681 635 245 219 191
168 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 2376 1700
Size: 676 5604
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2277 2340 2984 4384 5285 5547
Size: 197 926 334 63 644 1400 901 262
Cuts at: 5547 820
Size: 1553
Fragments arranged by size:
1553 1400 926 901 644 334 262 197
63
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2004 2785 3234 3370 3719 513
Size: 552 939 781 449 136 349 3074
Fragments arranged by size:
3074 939 781 552 449 349 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 3514 4307 4324 4477 4662 5107
Size: 1255 1089 845 793 17 153 185 445
Cuts at: 5107 325
Size: 1498
Fragments arranged by size:
1498 1255 1089 845 793 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 3102 4526
Size: 274 80 33 51 1443 387 738 1424
Cuts at: 4526 6238 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1424 738 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6240 6272 6240
Size: 32 6248
Tth111I GACn'n_nGTC
Cuts at: 1316 3143 3475 1316
Size: 1827 332 4121
Fragments arranged by size:
4121 1827 332
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2584 2584
Size: 6280
XhoI C'TCGA_G
Cuts at: 1943 2277 1943
Size: 334 5946
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI