Restriction map for
pFN21AE0309
(Circular) (Six-base) MAPSORT of: pFN21AE0309.seq Check: 186 from: 1 to: 6427
pFN21AE0309 6427 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2829 2891 2829
Size: 62 6365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2775 3770 1937
Size: 838 995 4594
Fragments arranged by size:
4594 995 838
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3319 4490 5874 6142 1632
Size: 1687 1171 1384 268 1917
Fragments arranged by size:
1917 1687 1384 1171 268
AfeI AGC'GCT
Cuts at: 3404 3404
Size: 6427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 3607 5156 3607
Size: 1549 4878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 4170 5742 4170
Size: 1572 4855
ApaLI G'TGCA_C
Cuts at: 2042 4484 5640 2042
Size: 2442 1156 2829
Fragments arranged by size:
2829 2442 1156
ApoI r'AATT_y
Cuts at: 1493 2147 3736 3962 4114 6223 1493
Size: 654 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 654 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 3752 1870
Size: 73 628 1181 4545
Fragments arranged by size:
4545 1181 628 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3375 3516 3576 3748 5203
Size: 325 217 627 1588 141 60 172 1455
Cuts at: 5203 618
Size: 1842
Fragments arranged by size:
1842 1588 1455 627 325 217 172 141
60
BanII G_rGCy'C
Cuts at: 1391 3257 3746 1391
Size: 1866 489 4072
Fragments arranged by size:
4072 1866 489
BbeI G_GCGC'C
Cuts at: 3580 3580
Size: 6427
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 928
Size: 971 657 4799
Fragments arranged by size:
4799 971 657
BbvCI CC'TCA_GC
Cuts at: 3194 3476 3194
Size: 282 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2338 2372 2842 2876 2921 2955 4618
Size: 34 401 34 470 34 45 34 1663
Cuts at: 4618 4652 1903
Size: 34 3678
Fragments arranged by size:
3678 1663 470 401 45 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 2280 1548
Size: 732 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2273 2991 3674
Size: 107 122 71 631 605 601 718 683
Cuts at: 3674 5038 136
Size: 1364 1525
Fragments arranged by size:
1525 1364 718 683 631 605 601 122
107 71
BglII A'GATC_T
Cuts at: 3156 3330 6422 3156
Size: 174 3092 3161
Fragments arranged by size:
3161 3092 174
BlpI GC'TnA_GC
Cuts at: 3053 3844 3053
Size: 791 5636
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2158 2269 4488 5644 1790
Size: 256 112 111 2219 1156 2573
Fragments arranged by size:
2573 2219 1156 256 112 111
BmgBI CAC'GTC
Cuts at: 1318 2122 2554 1318
Size: 804 432 5191
Fragments arranged by size:
5191 804 432
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 4158 5116
Size: 416 53 83 399 24 1284 1457 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
1753 1457 1284 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2291 5087 1239
Size: 812 240 2796 2579
Fragments arranged by size:
2796 2579 812 240
Bpu10I CC'TnA_GC
Cuts at: 2667 3194 3476 3617 2667
Size: 527 282 141 5477
Fragments arranged by size:
5477 527 282 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2579 4552 5417 5715 2114
Size: 465 1973 865 298 2826
Fragments arranged by size:
2826 1973 865 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3200 3559 5090 882
Size: 617 1701 359 1531 2219
Fragments arranged by size:
2219 1701 1531 617 359
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2379 3577 4234
Size: 53 83 186 936 549 297 1198 657
Cuts at: 4234 4616 5251 275
Size: 382 635 1451
Fragments arranged by size:
1451 1198 936 657 635 549 382 297
186 83 53
BsaWI w'CCGG_w
Cuts at: 2160 4860 5258 5532 5679 6090 2160
Size: 2700 398 274 147 411 2497
Fragments arranged by size:
2700 2497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6202
Fragments arranged by size:
6202 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2105 2612 3707 1186
Size: 853 66 507 1095 3906
Fragments arranged by size:
3906 1095 853 507 66
BseYI C'CCAG_C
Cuts at: 2949 3272 5630 6126 2949
Size: 323 2358 496 3250
Fragments arranged by size:
3250 2358 496 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2371 2836 1163
Size: 573 635 465 4754
Fragments arranged by size:
4754 635 573 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2641 3877 4638 5666 1540
Size: 275 186 640 1236 761 1028 2301
Fragments arranged by size:
2301 1236 1028 761 640 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3059 3257 4488 4573 5644 2046
Size: 1013 198 1231 85 1071 2829
Fragments arranged by size:
2829 1231 1071 1013 198 85
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
BsmBI CGTCTCn'nnnn_
Cuts at: 3215 3215
Size: 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2158 2269 3059 3257 3746 4488
Size: 399 256 112 111 790 198 489 742
Cuts at: 4488 4573 5644 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 790 742 489 399 256 198
112 111 85
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 2670 2829 2891 3782 844
Size: 1749 77 159 62 891 3489
Fragments arranged by size:
3489 1749 891 159 77 62
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2333 2937 3480 4922 5096 65
Size: 1183 1085 604 543 1442 174 1396
Fragments arranged by size:
1442 1396 1183 1085 604 543 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2811 3408 5071 5258 1700
Size: 110 1001 597 1663 187 2869
Fragments arranged by size:
2869 1663 1001 597 187 110
BsrGI T'GTAC_A
Cuts at: 96 3492 96
Size: 3396 3031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2115 794
Size: 1321 5106
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 3241 1184
Size: 2057 4370
BstYI r'GATC_y
Cuts at: 1972 2035 2094 3156 3330 3757 4124 4509 4526
Size: 63 59 1062 174 427 367 385 17
Cuts at: 4526 5978 6064 6076 6422 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1062 427 385 367 346 174
86 63 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3129 3201 3672 513
Size: 552 939 1125 72 471 3268
Fragments arranged by size:
3268 1125 939 552 471 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2845 487
Size: 683 56 1619 4069
Fragments arranged by size:
4069 1619 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 3346 3943 4737 4757 3271
Size: 75 597 794 20 4941
Fragments arranged by size:
4941 794 597 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DraIII CAC_nnn'GTG
Cuts at: 2506 2506
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2840 3040 3313
Size: 54 1425 50 275 18 1010 200 273
Cuts at: 3313 3564 3874 4763 6392 8
Size: 251 310 889 1629 43
Fragments arranged by size:
1629 1425 1010 889 310 275 273 251
200 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 3193 4357 1353
Size: 1167 673 1164 3423
Fragments arranged by size:
3423 1167 1164 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2675 5014 5388 5534 387
Size: 1064 138 1086 2339 374 146 1280
Fragments arranged by size:
2339 1280 1086 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3255 3255
Size: 6427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2291 3319 4490 5087 5874 6142
Size: 393 419 240 1028 1171 597 787 268
Cuts at: 6142 1239
Size: 1524
Fragments arranged by size:
1524 1171 1028 787 597 419 393 268
240
EcoO109I rG'GnC_Cy
Cuts at: 2654 2767 2887 2654
Size: 113 120 6194
Fragments arranged by size:
6194 120 113
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2573 2573
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2812 3406 3580 5574 1678
Size: 1134 594 174 1994 2531
Fragments arranged by size:
2531 1994 1134 594 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 2660 2692 2947
Size: 32 135 32 980 32 76 32 255
Cuts at: 2947 2979 3170 3202 3244 3276 3593 3625 5068
Size: 32 191 32 42 32 317 32 1443
Cuts at: 5068 5100 5142 5174 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 980 317 255 191 135 76
42 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KasI G'GCGC_C
Cuts at: 3576 3576
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 2944 4026 2944
Size: 1082 5345
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5541 5725 1305
Size: 4236 184 2007
Fragments arranged by size:
4236 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3566 10
Size: 54 1425 343 1734 2871
Fragments arranged by size:
2871 1734 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4385 4744 4903 518
Size: 648 3219 359 159 2042
Fragments arranged by size:
3219 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3386 3570 3843 4520 5668 5913 1606
Size: 756 1024 184 273 677 1148 245 2120
Fragments arranged by size:
2120 1148 1024 756 677 273 245 184
NaeI GCC'GGC
Cuts at: 2813 3410 2813
Size: 597 5830
NarI GG'CG_CC
Cuts at: 3577 3577
Size: 6427
NcoI C'CATG_G
Cuts at: 513 1065 2004 3129 3672 513
Size: 552 939 1125 543 3268
Fragments arranged by size:
3268 1125 939 552 543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NgoMIV G'CCGG_C
Cuts at: 2811 3408 2811
Size: 597 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3394 3785 5330 1132
Size: 97 2165 391 1545 2229
Fragments arranged by size:
2229 2165 1545 391 97
PasI CC'CwG_GG
Cuts at: 1348 3427 1348
Size: 2079 4348
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2949 1108
Size: 1841 4586
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2654 2767 2654
Size: 113 6314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2361 2367 3385 3779 830
Size: 1531 6 1018 394 3478
Fragments arranged by size:
3478 1531 1018 394 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2362 3386 3570 2362
Size: 1024 184 5219
Fragments arranged by size:
5219 1024 184
SacI G_AGCT'C
Cuts at: 3257 3257
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SanDI GG'GwC_CC
Cuts at: 2654 2654
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SexAI A'CCwGG_T
Cuts at: 2739 2769 2739
Size: 30 6397
SfcI C'TryA_G
Cuts at: 826 1045 2357 2363 2880 3381 3775 4910 5591
Size: 219 1312 6 517 501 394 1135 681
Cuts at: 5591 5782 826
Size: 191 1471
Fragments arranged by size:
1471 1312 1135 681 517 501 394 219
191 6
SfoI GGC'GCC
Cuts at: 3578 3578
Size: 6427
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 2558 4531 5432 5694 820
Size: 197 926 186 429 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 429 262 197 186
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3394 3785 1132
Size: 2262 391 3774
Fragments arranged by size:
3774 2262 391
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 3068 1517
Size: 1551 4876
StyI C'CwwG_G
Cuts at: 513 1065 2004 2544 3129 3672 3866 513
Size: 552 939 540 585 543 194 3074
Fragments arranged by size:
3074 939 585 552 543 540 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2417 2427 4454 4471 4624 4809 5254
Size: 1255 837 10 2027 17 153 185 445
Cuts at: 5254 325
Size: 1498
Fragments arranged by size:
2027 1498 1255 837 445 185 153 17
10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2725 3492 4673
Size: 274 80 33 51 1443 748 767 1181
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1181 767 748 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 2406 4556 2406
Size: 2150 4277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SapI SfiI SrfI SwaI XcmI