Restriction map for
pFN21AE0308
(Circular) (Six-base) MAPSORT of: pFN21AE0308.seq Check: 2714 from: 1 to: 6262
pFN21AE0308 6262 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3460 3605 1937
Size: 1523 145 4594
Fragments arranged by size:
4594 1523 145
Acc65I G'GTAC_C
Cuts at: 2267 3583 2267
Size: 1316 4946
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2943 3398 4325 5709 5977 1632
Size: 806 505 455 927 1384 268 1917
Fragments arranged by size:
1917 1384 927 806 505 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AlwNI CAG_nnn'CTG
Cuts at: 3019 3368 3377 3421 4005 5577 3019
Size: 349 9 44 584 1572 3704
Fragments arranged by size:
3704 1572 584 349 44 9
ApaI G_GGCC'C
Cuts at: 2582 2582
Size: 6262
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 3507 3571 3797 3949 6058 1493
Size: 2014 64 226 152 2109 1697
Fragments arranged by size:
2109 2014 1697 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 3587 1870
Size: 73 1644 4545
Fragments arranged by size:
4545 1644 73
BamHI G'GATC_C
Cuts at: 2856 3592 3959 2856
Size: 736 367 5159
Fragments arranged by size:
5159 736 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2267 3583 5038 618
Size: 325 217 627 480 1316 1455 1842
Fragments arranged by size:
1842 1455 1316 627 480 325 217
BanII G_rGCy'C
Cuts at: 1391 2582 2709 2737 2868 3581 1391
Size: 1191 127 28 131 713 4072
Fragments arranged by size:
4072 1191 713 131 127 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2394 2428 4453 4487 1903
Size: 34 457 34 2025 34 3678
Fragments arranged by size:
3678 2025 457 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BfrBI ATG'CAT
Cuts at: 2948 2948
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 2651 3679 2651
Size: 1028 5234
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2582 4323 5479 1790
Size: 339 453 1741 1156 2573
Fragments arranged by size:
2573 1741 1156 453 339
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3993 4951 442
Size: 416 53 83 399 24 2576 958 1753
Fragments arranged by size:
2576 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2196 2196
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2104 2168 2265 2563 2587 2771 4922 1239
Size: 865 64 97 298 24 184 2151 2579
Fragments arranged by size:
2579 2151 865 298 184 97 64 24
Bpu10I CC'TnA_GC
Cuts at: 2505 2505
Size: 6262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 4387 5252 5550 2792
Size: 1595 865 298 3504
Fragments arranged by size:
3504 1595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4925 882
Size: 617 3426 2219
Fragments arranged by size:
3426 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2095 4069 4451 5086
Size: 53 83 186 936 562 1974 382 635
Cuts at: 5086 275
Size: 1451
Fragments arranged by size:
1974 1451 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4695 5093 5367 5514 5925 4695
Size: 398 274 147 411 5032
Fragments arranged by size:
5032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6262
BseYI C'CCAG_C
Cuts at: 2122 2597 5465 5961 2122
Size: 475 2868 496 2423
Fragments arranged by size:
2868 2423 496 475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5689
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2778 4323 4408 5479 2778
Size: 1545 85 1071 3561
Fragments arranged by size:
3561 1545 1071 85
BsmI GAATG_Cn'
Cuts at: 2679 3773 3866 2679
Size: 1094 93 5075
Fragments arranged by size:
5075 1094 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2582 2709 2737 2778 2868 3581
Size: 399 339 453 127 28 41 90 713
Cuts at: 3581 4323 4408 5479 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 713 453 399 339 127
90 85 41 28
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 3088 4151 5881 3088
Size: 1063 1730 3469
Fragments arranged by size:
3469 1730 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2730 4149 1394
Size: 429 907 1419 3507
Fragments arranged by size:
3507 1419 907 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2302 4757 4931 65
Size: 1183 1054 2455 174 1396
Fragments arranged by size:
2455 1396 1183 1054 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2356 4906 5093 1700
Size: 110 281 265 2550 187 2869
Fragments arranged by size:
2869 2550 281 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 2325 3190 96
Size: 2229 865 3168
Fragments arranged by size:
3168 2229 865
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2417 794
Size: 1623 4639
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2297 2146
Size: 151 6111
BstYI r'GATC_y
Cuts at: 1972 2622 2856 3592 3959 4344 4361 5813 5899
Size: 650 234 736 367 385 17 1452 86
Cuts at: 5899 5911 6257 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 736 650 385 367 346 234
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2066 2368 513
Size: 552 1001 302 4407
Fragments arranged by size:
4407 1001 552 302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2201 487
Size: 683 56 975 4548
Fragments arranged by size:
4548 975 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 2412 2703 3778 4572 4592 2127
Size: 285 291 1075 794 20 3797
Fragments arranged by size:
3797 1075 794 291 285 20
ClaI AT'CG_AT
Cuts at: 1554 3483 1554
Size: 1929 4333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2350 2385 529
Size: 35 1786 35 4406
Fragments arranged by size:
4406 1786 35 35
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DraIII CAC_nnn'GTG
Cuts at: 2133 2133
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2881 5269 809
Size: 507 1565 2388 1802
Fragments arranged by size:
2388 1802 1565 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2917 3043
Size: 54 1425 50 275 18 528 559 126
Cuts at: 3043 3709 4598 6227 8
Size: 666 889 1629 43
Fragments arranged by size:
1629 1425 889 666 559 528 275 126
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3380 4192 1353
Size: 2027 812 3423
Fragments arranged by size:
3423 2027 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2702 4849 5223 5369 387
Size: 1064 138 1113 2147 374 146 1280
Fragments arranged by size:
2147 1280 1113 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2168 2265 2438 2563 2587 2771
Size: 393 472 64 97 173 125 24 184
Cuts at: 2771 2943 3398 4325 4922 5709 5977 1239
Size: 172 455 927 597 787 268 1524
Fragments arranged by size:
1524 927 787 597 472 455 393 268
184 173 172 125 97 64 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3063 3063
Size: 6262
EcoO109I rG'GnC_Cy
Cuts at: 2103 2175 2578 2874 2103
Size: 72 403 296 5491
Fragments arranged by size:
5491 403 296 72
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 3319 2833
Size: 486 5776
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2640 2772 5409 1678
Size: 962 132 2637 2531
Fragments arranged by size:
2637 2531 962 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3121 3153 4903 4935 4977
Size: 32 135 32 1549 32 1750 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 1750 1549 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3476 748
Size: 2728 3534
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 2271 3587 2271
Size: 1316 4946
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2422 5376 5560 1305
Size: 1117 2954 184 2007
Fragments arranged by size:
2954 2007 1117 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4440
Fragments arranged by size:
4440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3087 4220 4579 4738 518
Size: 648 1921 1133 359 159 2042
Fragments arranged by size:
2042 1921 1133 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2781 3678 4355 5503 5748 1606
Size: 462 713 897 677 1148 245 2120
Fragments arranged by size:
2120 1148 897 713 677 462 245
NaeI GCC'GGC
Cuts at: 2093 2358 2093
Size: 265 5997
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5710
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NgoMIV G'CCGG_C
Cuts at: 2091 2356 2091
Size: 265 5997
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NsiI A_TGCA'T
Cuts at: 2950 2950
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2405 3620 5165 1132
Size: 97 1176 1215 1545 2229
Fragments arranged by size:
2229 1545 1215 1176 97
PasI CC'CwG_GG
Cuts at: 1348 2290 2423 1348
Size: 942 133 5187
Fragments arranged by size:
5187 942 133
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PfoI T'CCnGG_A
Cuts at: 2560 2560
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2103 2874 2103
Size: 771 5491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3135 3832 3135
Size: 697 5565
PspOMI G'GGCC_C
Cuts at: 2578 2578
Size: 6262
PstI C_TGCA'G
Cuts at: 830 2313 2595 3373 3614 830
Size: 1483 282 778 241 3478
Fragments arranged by size:
3478 1483 778 282 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
SacII CC_GC'GG
Cuts at: 2069 2069
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SapI GCTCTTCn'nnn_
Cuts at: 3380 3380
Size: 6262
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 2517 2517
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 2309 2487 2591 2626 3369 3610 4745
Size: 219 1264 178 104 35 743 241 1135
Cuts at: 4745 5426 5617 826
Size: 681 191 1471
Fragments arranged by size:
1471 1264 1135 743 681 241 219 191
178 104 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 4366 5267 5529 820
Size: 197 926 864 1559 901 262 1553
Fragments arranged by size:
1559 1553 926 901 864 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 2405 3620 1132
Size: 1273 1215 3774
Fragments arranged by size:
3774 1273 1215
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2099 2817 3223 3269 3701 513
Size: 552 1034 718 406 46 432 3074
Fragments arranged by size:
3074 1034 718 552 432 406 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 4289 4306 4459 4644 5089 325
Size: 1255 1410 1299 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1299 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 3071 3190
Size: 274 80 33 51 1443 348 746 119
Cuts at: 3190 4508 6220 96
Size: 1318 1712 138
Fragments arranged by size:
1712 1443 1318 746 348 274 138 119
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3234 3266 6222 6254 3234
Size: 32 2956 32 3242
Fragments arranged by size:
3242 2956 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
KasI NarI NruI PacI PmlI PspXI PsrI PvuII
RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI