Restriction map for
pFN21AE0307
(Circular) (Six-base) MAPSORT of: pFN21AE0307.seq Check: 4130 from: 1 to: 6757
pFN21AE0307 6757 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4053 4100 1937
Size: 2116 47 4594
Fragments arranged by size:
4594 2116 47
Acc65I G'GTAC_C
Cuts at: 4078 4078
Size: 6757
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 2343 2687 2708 3020 3071 3714 4820
Size: 561 150 344 21 312 51 643 1106
Cuts at: 4820 6204 6472 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1106 643 561 344 312 268
150 51 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 5656 6633 1203
Size: 4453 977 1327
Fragments arranged by size:
4453 1327 977
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 2929 4500 6072 2929
Size: 1571 1572 3614
Fragments arranged by size:
3614 1572 1571
ApaI G_GGCC'C
Cuts at: 4014 4014
Size: 6757
ApaLI G'TGCA_C
Cuts at: 2156 4814 5970 2156
Size: 2658 1156 2943
Fragments arranged by size:
2943 2658 1156
ApoI r'AATT_y
Cuts at: 1493 2580 3110 4066 4292 4444 6553 1493
Size: 1087 530 956 226 152 2109 1697
Fragments arranged by size:
2109 1697 1087 956 530 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 4082 1870
Size: 73 2139 4545
Fragments arranged by size:
4545 2139 73
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3937 4078 5533 618
Size: 325 217 627 2150 141 1455 1842
Fragments arranged by size:
2150 1842 1455 627 325 217 141
BanII G_rGCy'C
Cuts at: 1391 2249 2379 2877 3954 4014 4076 1391
Size: 858 130 498 1077 60 62 4072
Fragments arranged by size:
4072 1077 858 498 130 62 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 928
Size: 971 303 5483
Fragments arranged by size:
5483 971 303
BbvCI CC'TCA_GC
Cuts at: 4003 4003
Size: 6757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3128 3162 4948 4982 1903
Size: 34 1191 34 1786 34 3678
Fragments arranged by size:
3678 1786 1191 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4649 5859 4649
Size: 1210 5547
BclI T'GATC_A
Cuts at: 1548 3802 1548
Size: 2254 4503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3851 3958
Size: 107 122 71 631 605 337 1842 107
Cuts at: 3958 5368 136
Size: 1410 1525
Fragments arranged by size:
1842 1525 1410 631 605 337 122 107
107 71
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 3078 4174 3078
Size: 1096 5661
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2713 4014 4818 5974 1790
Size: 370 553 1301 804 1156 2573
Fragments arranged by size:
2573 1301 1156 804 553 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2073 2120 3948
Size: 416 53 83 399 24 656 47 1828
Cuts at: 3948 4488 5446 442
Size: 540 958 1753
Fragments arranged by size:
1828 1753 958 656 540 416 399 83
53 47 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2645
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2114 2373 3885 5417 1239
Size: 801 74 259 1512 1532 2579
Fragments arranged by size:
2579 1532 1512 801 259 74
Bpu10I CC'TnA_GC
Cuts at: 4003 4003
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3602 4882 5747 6045 2966
Size: 636 1280 865 298 3678
Fragments arranged by size:
3678 1280 865 636 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3785 5420 882
Size: 617 2286 1635 2219
Fragments arranged by size:
2286 2219 1635 617
BsaAI yAC'GTr
Cuts at: 493 1204 2714 4548 493
Size: 711 1510 1834 2702
Fragments arranged by size:
2702 1834 1510 711
BsaBI GATnn'nnATC
Cuts at: 2529 3311 6751 2529
Size: 782 3440 2535
Fragments arranged by size:
3440 2535 782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3609 3809 4050 4564
Size: 53 83 186 936 2076 200 241 514
Cuts at: 4564 4946 5581 275
Size: 382 635 1451
Fragments arranged by size:
2076 1451 936 635 514 382 241 200
186 83 53
BsaWI w'CCGG_w
Cuts at: 2476 3836 5190 5588 5862 6009 6420 2476
Size: 1360 1354 398 274 147 411 2813
Fragments arranged by size:
2813 1360 1354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 1178
Size: 30 165 30 1236 30 5266
Fragments arranged by size:
5266 1236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2897 2993 3491 3675 3998 1186
Size: 1363 348 96 498 184 323 3945
Fragments arranged by size:
3945 1363 498 348 323 184 96
BseYI C'CCAG_C
Cuts at: 4007 4014 5960 6456 4007
Size: 7 1946 496 4308
Fragments arranged by size:
4308 1946 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 4968 5996 1540
Size: 275 186 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2877 3122 3796 3924 4818 4903 5974 2160
Size: 717 245 674 128 894 85 1071 2943
Fragments arranged by size:
2943 1071 894 717 674 245 128 85
BsmI GAATG_Cn'
Cuts at: 2281 4268 4361 2281
Size: 1987 93 4677
Fragments arranged by size:
4677 1987 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3375 3454 3375
Size: 79 6678
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2249 2379 2713 2877 3122 3796
Size: 399 370 89 130 334 164 245 674
Cuts at: 3796 3924 3954 4014 4076 4818 4903 5974 1391
Size: 128 30 60 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 399 370 334 245
164 130 128 89 85 62 60 30
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 3525 4646 6376 3525
Size: 1121 1730 3906
Fragments arranged by size:
3906 1730 1121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2451 4112 844
Size: 1607 1661 3489
Fragments arranged by size:
3489 1661 1607
BsrBI CCG'CTC
Cuts at: 1394 1823 3614 4644 1394
Size: 429 1791 1030 3507
Fragments arranged by size:
3507 1791 1030 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5252 5426 65
Size: 1183 4004 174 1396
Fragments arranged by size:
4004 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5401 5588 1700
Size: 110 3591 187 2869
Fragments arranged by size:
3591 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 3969 4817 5829 3969
Size: 848 1012 4897
Fragments arranged by size:
4897 1012 848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6757
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6757
BstYI r'GATC_y
Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752
Size: 2115 367 385 17 1452 86 12 346
Cuts at: 6752 1972
Size: 1977
Fragments arranged by size:
2115 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3687 3687
Size: 6757
BtgI C'CryG_G
Cuts at: 513 1065 2004 3933 513
Size: 552 939 1929 3337
Fragments arranged by size:
3337 1929 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 3715 4273 5067 5087 3715
Size: 558 794 20 5385
Fragments arranged by size:
5385 794 558 20
ClaI AT'CG_AT
Cuts at: 1554 2862 1554
Size: 1308 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 2905 4061 2905
Size: 1156 5601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 3513 4204
Size: 54 1425 50 275 18 277 1406 691
Cuts at: 4204 5093 6722 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1406 889 691 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2319 2329 2688 2718 3261 3345 3426
Size: 816 150 10 359 30 543 84 81
Cuts at: 3426 3912 4687 1353
Size: 486 775 3423
Fragments arranged by size:
3423 816 775 543 486 359 150 84
81 30 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5344 5718 5864 387
Size: 1064 138 3755 374 146 1280
Fragments arranged by size:
3755 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2875 2875
Size: 6757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2114 2193 2343 2373 2687 2708
Size: 393 408 74 79 150 30 314 21
Cuts at: 2708 3020 3071 3714 3885 4820 5417 6204 6472
Size: 312 51 643 171 935 597 787 268
Cuts at: 6472 1239
Size: 1524
Fragments arranged by size:
1524 935 787 643 597 408 393 314
312 268 171 150 79 74 51 30
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 6757
EcoO109I rG'GnC_Cy
Cuts at: 3844 3999 4011 3844
Size: 155 12 6590
Fragments arranged by size:
6590 155 12
EcoRI G'AATT_C
Cuts at: 3110 4066 4444 3110
Size: 956 378 5423
Fragments arranged by size:
5423 956 378
EcoRV GAT'ATC
Cuts at: 2167 2167
Size: 6757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2717 2717
Size: 6757
FspI TGC'GCA
Cuts at: 3910 5263 3910
Size: 1353 5404
HaeII r_GCGC'y
Cuts at: 1678 2702 5904 1678
Size: 1024 3202 2531
Fragments arranged by size:
3202 2531 1024
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 3425 3457 3520
Size: 32 135 32 482 32 1339 32 63
Cuts at: 3520 3552 3794 3826 5398 5430 5472 5504 1373
Size: 32 242 32 1572 32 42 32 2626
Fragments arranged by size:
2626 1572 1339 482 242 135 63 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 3549 4054 4101 4347 669
Size: 988 281 111 1500 505 47 246 3079
Fragments arranged by size:
3079 1500 988 505 281 246 111 47
HindIII A'AGCT_T
Cuts at: 748 2942 3831 748
Size: 2194 889 3674
Fragments arranged by size:
3674 2194 889
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 4082 4082
Size: 6757
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2144 2155 3234 3769 5871 6055 1305
Size: 839 11 1079 535 2102 184 2007
Fragments arranged by size:
2102 2007 1079 839 535 184 11
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2109 3515 10
Size: 54 1425 343 277 1406 3252
Fragments arranged by size:
3252 1425 1406 343 277 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4715 5074 5233 518
Size: 648 3549 359 159 2042
Fragments arranged by size:
3549 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2191 2707 4007 4173 4850 5998 6243
Size: 430 155 516 1300 166 677 1148 245
Cuts at: 6243 1606
Size: 2120
Fragments arranged by size:
2120 1300 1148 677 516 430 245 166
155
NcoI C'CATG_G
Cuts at: 513 1065 2004 3933 513
Size: 552 939 1929 3337
Fragments arranged by size:
3337 1929 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6757
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NspI r_CATG'y
Cuts at: 1132 1229 3909 3948 4115 5660 1132
Size: 97 2680 39 167 1545 2229
Fragments arranged by size:
2680 2229 1545 167 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6757
PfoI T'CCnGG_A
Cuts at: 3812 3812
Size: 6757
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PmlI CAC'GTG
Cuts at: 2714 2714
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 6339 6371 4826
Size: 32 1481 32 5212
Fragments arranged by size:
5212 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3411 1613
Size: 1798 4959
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PspOMI G'GGCC_C
Cuts at: 4010 4010
Size: 6757
PstI C_TGCA'G
Cuts at: 830 2670 2922 3075 4109 830
Size: 1840 252 153 1034 3478
Fragments arranged by size:
3478 1840 1034 252 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
PvuII CAG'CTG
Cuts at: 2191 4007 2191
Size: 1816 4941
SacI G_AGCT'C
Cuts at: 2877 2877
Size: 6757
SalI G'TCGA_C
Cuts at: 1936 4052 4099 1936
Size: 2116 47 4594
Fragments arranged by size:
4594 2116 47
SapI GCTCTTCn'nnn_
Cuts at: 2169 3426 3912 2169
Size: 1257 486 5014
Fragments arranged by size:
5014 1257 486
SbfI CC_TGCA'GG
Cuts at: 3075 4109 3075
Size: 1034 5723
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SfcI C'TryA_G
Cuts at: 826 1045 2666 2918 3071 3872 4105 5240 5921
Size: 219 1621 252 153 801 233 1135 681
Cuts at: 5921 6112 826
Size: 191 1471
Fragments arranged by size:
1621 1471 1135 801 681 252 233 219
191 153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 4084 1872
Size: 2212 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2945 3617 4861 5762 6024 820
Size: 197 926 1002 672 1244 901 262 1553
Fragments arranged by size:
1553 1244 1002 926 901 672 262 197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 3909 3948 4115 1132
Size: 2777 39 167 3774
Fragments arranged by size:
3774 2777 167 39
SspI AAT'ATT
Cuts at: 5 52 2901 3005 4681 5
Size: 47 2849 104 1676 2081
Fragments arranged by size:
2849 2081 1676 104 47
StuI AGG'CCT
Cuts at: 1517 3866 1517
Size: 2349 4408
StyI C'CwwG_G
Cuts at: 513 1065 2004 3729 3933 4196 513
Size: 552 939 1725 204 263 3074
Fragments arranged by size:
3074 1725 939 552 263 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2377 4784 4801 4954 5139 5584 325
Size: 1255 797 2407 17 153 185 445 1498
Fragments arranged by size:
2407 1498 1255 797 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5003 6715 96
Size: 274 80 33 51 1443 3026 1712 138
Fragments arranged by size:
3026 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2639 2671 6717 6749 2639
Size: 32 4046 32 2647
Fragments arranged by size:
4046 2647 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6757
XmaI C'CCGG_G
Cuts at: 1870 4082 1870
Size: 2212 4545
XmnI GAAnn'nnTTC
Cuts at: 4886 4886
Size: 6757
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI