(Circular) (Six-base) MAPSORT of: pFN21AE0307.seq Check: 4130 from: 1 to: 6757 pFN21AE0307 6757 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4053 4100 1937 Size: 2116 47 4594 Fragments arranged by size: 4594 2116 47 Acc65I G'GTAC_C Cuts at: 4078 4078 Size: 6757 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 2343 2687 2708 3020 3071 3714 4820 Size: 561 150 344 21 312 51 643 1106 Cuts at: 4820 6204 6472 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1106 643 561 344 312 268 150 51 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 5656 6633 1203 Size: 4453 977 1327 Fragments arranged by size: 4453 1327 977 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 2929 4500 6072 2929 Size: 1571 1572 3614 Fragments arranged by size: 3614 1572 1571 ApaI G_GGCC'C Cuts at: 4014 4014 Size: 6757 ApaLI G'TGCA_C Cuts at: 2156 4814 5970 2156 Size: 2658 1156 2943 Fragments arranged by size: 2943 2658 1156 ApoI r'AATT_y Cuts at: 1493 2580 3110 4066 4292 4444 6553 1493 Size: 1087 530 956 226 152 2109 1697 Fragments arranged by size: 2109 1697 1087 956 530 226 152 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 4082 1870 Size: 73 2139 4545 Fragments arranged by size: 4545 2139 73 BamHI G'GATC_C Cuts at: 4087 4454 4087 Size: 367 6390 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3937 4078 5533 618 Size: 325 217 627 2150 141 1455 1842 Fragments arranged by size: 2150 1842 1455 627 325 217 141 BanII G_rGCy'C Cuts at: 1391 2249 2379 2877 3954 4014 4076 1391 Size: 858 130 498 1077 60 62 4072 Fragments arranged by size: 4072 1077 858 498 130 62 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 928 Size: 971 303 5483 Fragments arranged by size: 5483 971 303 BbvCI CC'TCA_GC Cuts at: 4003 4003 Size: 6757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3128 3162 4948 4982 1903 Size: 34 1191 34 1786 34 3678 Fragments arranged by size: 3678 1786 1191 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4649 5859 4649 Size: 1210 5547 BclI T'GATC_A Cuts at: 1548 3802 1548 Size: 2254 4503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3851 3958 Size: 107 122 71 631 605 337 1842 107 Cuts at: 3958 5368 136 Size: 1410 1525 Fragments arranged by size: 1842 1525 1410 631 605 337 122 107 107 71 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 3078 4174 3078 Size: 1096 5661 Bme1580I G_kGCm'C Cuts at: 1790 2160 2713 4014 4818 5974 1790 Size: 370 553 1301 804 1156 2573 Fragments arranged by size: 2573 1301 1156 804 553 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2073 2120 3948 Size: 416 53 83 399 24 656 47 1828 Cuts at: 3948 4488 5446 442 Size: 540 958 1753 Fragments arranged by size: 1828 1753 958 656 540 416 399 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2645 2645 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2114 2373 3885 5417 1239 Size: 801 74 259 1512 1532 2579 Fragments arranged by size: 2579 1532 1512 801 259 74 Bpu10I CC'TnA_GC Cuts at: 4003 4003 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 3602 4882 5747 6045 2966 Size: 636 1280 865 298 3678 Fragments arranged by size: 3678 1280 865 636 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3785 5420 882 Size: 617 2286 1635 2219 Fragments arranged by size: 2286 2219 1635 617 BsaAI yAC'GTr Cuts at: 493 1204 2714 4548 493 Size: 711 1510 1834 2702 Fragments arranged by size: 2702 1834 1510 711 BsaBI GATnn'nnATC Cuts at: 2529 3311 6751 2529 Size: 782 3440 2535 Fragments arranged by size: 3440 2535 782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3609 3809 4050 4564 Size: 53 83 186 936 2076 200 241 514 Cuts at: 4564 4946 5581 275 Size: 382 635 1451 Fragments arranged by size: 2076 1451 936 635 514 382 241 200 186 83 53 BsaWI w'CCGG_w Cuts at: 2476 3836 5190 5588 5862 6009 6420 2476 Size: 1360 1354 398 274 147 411 2813 Fragments arranged by size: 2813 1360 1354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 1178 Size: 30 165 30 1236 30 5266 Fragments arranged by size: 5266 1236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2897 2993 3491 3675 3998 1186 Size: 1363 348 96 498 184 323 3945 Fragments arranged by size: 3945 1363 498 348 323 184 96 BseYI C'CCAG_C Cuts at: 4007 4014 5960 6456 4007 Size: 7 1946 496 4308 Fragments arranged by size: 4308 1946 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 4968 5996 1540 Size: 275 186 2206 761 1028 2301 Fragments arranged by size: 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2877 3122 3796 3924 4818 4903 5974 2160 Size: 717 245 674 128 894 85 1071 2943 Fragments arranged by size: 2943 1071 894 717 674 245 128 85 BsmI GAATG_Cn' Cuts at: 2281 4268 4361 2281 Size: 1987 93 4677 Fragments arranged by size: 4677 1987 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3375 3454 3375 Size: 79 6678 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2249 2379 2713 2877 3122 3796 Size: 399 370 89 130 334 164 245 674 Cuts at: 3796 3924 3954 4014 4076 4818 4903 5974 1391 Size: 128 30 60 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 399 370 334 245 164 130 128 89 85 62 60 30 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 3525 4646 6376 3525 Size: 1121 1730 3906 Fragments arranged by size: 3906 1730 1121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2451 4112 844 Size: 1607 1661 3489 Fragments arranged by size: 3489 1661 1607 BsrBI CCG'CTC Cuts at: 1394 1823 3614 4644 1394 Size: 429 1791 1030 3507 Fragments arranged by size: 3507 1791 1030 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5252 5426 65 Size: 1183 4004 174 1396 Fragments arranged by size: 4004 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5401 5588 1700 Size: 110 3591 187 2869 Fragments arranged by size: 3591 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6757 BssSI C'ACGA_G Cuts at: 3969 4817 5829 3969 Size: 848 1012 4897 Fragments arranged by size: 4897 1012 848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6757 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6757 BstYI r'GATC_y Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752 Size: 2115 367 385 17 1452 86 12 346 Cuts at: 6752 1972 Size: 1977 Fragments arranged by size: 2115 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3687 3687 Size: 6757 BtgI C'CryG_G Cuts at: 513 1065 2004 3933 513 Size: 552 939 1929 3337 Fragments arranged by size: 3337 1929 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 3715 4273 5067 5087 3715 Size: 558 794 20 5385 Fragments arranged by size: 5385 794 558 20 ClaI AT'CG_AT Cuts at: 1554 2862 1554 Size: 1308 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 2905 4061 2905 Size: 1156 5601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 3513 4204 Size: 54 1425 50 275 18 277 1406 691 Cuts at: 4204 5093 6722 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1406 889 691 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4204 1537 Size: 275 2392 4090 Fragments arranged by size: 4090 2392 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 2319 2329 2688 2718 3261 3345 3426 Size: 816 150 10 359 30 543 84 81 Cuts at: 3426 3912 4687 1353 Size: 486 775 3423 Fragments arranged by size: 3423 816 775 543 486 359 150 84 81 30 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5344 5718 5864 387 Size: 1064 138 3755 374 146 1280 Fragments arranged by size: 3755 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2875 2875 Size: 6757 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2114 2193 2343 2373 2687 2708 Size: 393 408 74 79 150 30 314 21 Cuts at: 2708 3020 3071 3714 3885 4820 5417 6204 6472 Size: 312 51 643 171 935 597 787 268 Cuts at: 6472 1239 Size: 1524 Fragments arranged by size: 1524 935 787 643 597 408 393 314 312 268 171 150 79 74 51 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 6757 EcoO109I rG'GnC_Cy Cuts at: 3844 3999 4011 3844 Size: 155 12 6590 Fragments arranged by size: 6590 155 12 EcoRI G'AATT_C Cuts at: 3110 4066 4444 3110 Size: 956 378 5423 Fragments arranged by size: 5423 956 378 EcoRV GAT'ATC Cuts at: 2167 2167 Size: 6757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2717 2717 Size: 6757 FspI TGC'GCA Cuts at: 3910 5263 3910 Size: 1353 5404 HaeII r_GCGC'y Cuts at: 1678 2702 5904 1678 Size: 1024 3202 2531 Fragments arranged by size: 3202 2531 1024 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 3425 3457 3520 Size: 32 135 32 482 32 1339 32 63 Cuts at: 3520 3552 3794 3826 5398 5430 5472 5504 1373 Size: 32 242 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 1339 482 242 135 63 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2049 3549 4054 4101 4347 669 Size: 988 281 111 1500 505 47 246 3079 Fragments arranged by size: 3079 1500 988 505 281 246 111 47 HindIII A'AGCT_T Cuts at: 748 2942 3831 748 Size: 2194 889 3674 Fragments arranged by size: 3674 2194 889 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 4082 4082 Size: 6757 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2144 2155 3234 3769 5871 6055 1305 Size: 839 11 1079 535 2102 184 2007 Fragments arranged by size: 2102 2007 1079 839 535 184 11 MscI TGG'CCA Cuts at: 10 64 1489 1832 2109 3515 10 Size: 54 1425 343 277 1406 3252 Fragments arranged by size: 3252 1425 1406 343 277 54 MslI CAynn'nnrTG Cuts at: 518 1166 4715 5074 5233 518 Size: 648 3549 359 159 2042 Fragments arranged by size: 3549 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2191 2707 4007 4173 4850 5998 6243 Size: 430 155 516 1300 166 677 1148 245 Cuts at: 6243 1606 Size: 2120 Fragments arranged by size: 2120 1300 1148 677 516 430 245 166 155 NcoI C'CATG_G Cuts at: 513 1065 2004 3933 513 Size: 552 939 1929 3337 Fragments arranged by size: 3337 1929 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6757 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 4204 4204 Size: 6757 NspI r_CATG'y Cuts at: 1132 1229 3909 3948 4115 5660 1132 Size: 97 2680 39 167 1545 2229 Fragments arranged by size: 2680 2229 1545 167 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6757 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6757 PfoI T'CCnGG_A Cuts at: 3812 3812 Size: 6757 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PmlI CAC'GTG Cuts at: 2714 2714 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4826 4858 6339 6371 4826 Size: 32 1481 32 5212 Fragments arranged by size: 5212 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3411 1613 Size: 1798 4959 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PspOMI G'GGCC_C Cuts at: 4010 4010 Size: 6757 PstI C_TGCA'G Cuts at: 830 2670 2922 3075 4109 830 Size: 1840 252 153 1034 3478 Fragments arranged by size: 3478 1840 1034 252 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 PvuII CAG'CTG Cuts at: 2191 4007 2191 Size: 1816 4941 SacI G_AGCT'C Cuts at: 2877 2877 Size: 6757 SalI G'TCGA_C Cuts at: 1936 4052 4099 1936 Size: 2116 47 4594 Fragments arranged by size: 4594 2116 47 SapI GCTCTTCn'nnn_ Cuts at: 2169 3426 3912 2169 Size: 1257 486 5014 Fragments arranged by size: 5014 1257 486 SbfI CC_TGCA'GG Cuts at: 3075 4109 3075 Size: 1034 5723 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SfcI C'TryA_G Cuts at: 826 1045 2666 2918 3071 3872 4105 5240 5921 Size: 219 1621 252 153 801 233 1135 681 Cuts at: 5921 6112 826 Size: 191 1471 Fragments arranged by size: 1621 1471 1135 801 681 252 233 219 191 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 4084 1872 Size: 2212 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2945 3617 4861 5762 6024 820 Size: 197 926 1002 672 1244 901 262 1553 Fragments arranged by size: 1553 1244 1002 926 901 672 262 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 3909 3948 4115 1132 Size: 2777 39 167 3774 Fragments arranged by size: 3774 2777 167 39 SspI AAT'ATT Cuts at: 5 52 2901 3005 4681 5 Size: 47 2849 104 1676 2081 Fragments arranged by size: 2849 2081 1676 104 47 StuI AGG'CCT Cuts at: 1517 3866 1517 Size: 2349 4408 StyI C'CwwG_G Cuts at: 513 1065 2004 3729 3933 4196 513 Size: 552 939 1725 204 263 3074 Fragments arranged by size: 3074 1725 939 552 263 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2377 4784 4801 4954 5139 5584 325 Size: 1255 797 2407 17 153 185 445 1498 Fragments arranged by size: 2407 1498 1255 797 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5003 6715 96 Size: 274 80 33 51 1443 3026 1712 138 Fragments arranged by size: 3026 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2639 2671 6717 6749 2639 Size: 32 4046 32 2647 Fragments arranged by size: 4046 2647 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6757 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6757 XmaI C'CCGG_G Cuts at: 1870 4082 1870 Size: 2212 4545 XmnI GAAnn'nnTTC Cuts at: 4886 4886 Size: 6757 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI