Restriction map for
pFN21AE0301
(Circular) (Six-base) MAPSORT of: pFN21AE0301.seq Check: 6692 from: 1 to: 6232
pFN21AE0301 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2008 2008
Size: 6232
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 3531 4359 4732 1563
Size: 1968 828 373 3063
Fragments arranged by size:
3063 1968 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 4295 5679 5947 1632
Size: 1172 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 1172 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 3165 3295 5131 6108 1203
Size: 1962 130 1836 977 1327
Fragments arranged by size:
1962 1836 1327 977 130
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 1184
Size: 1782 4450
AlwNI CAG_nnn'CTG
Cuts at: 2023 2237 2507 2633 3401 3975 5547 2023
Size: 214 270 126 768 574 1572 2708
Fragments arranged by size:
2708 1572 768 574 270 214 126
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2852 3449 3557 1870
Size: 73 909 597 108 4545
Fragments arranged by size:
4545 909 597 108 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2379 2412 2379
Size: 33 6199
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3551 1391
Size: 2160 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3103 928
Size: 971 1204 4057
Fragments arranged by size:
4057 1204 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2380 2540 4124 5334 2380
Size: 160 1584 1210 3278
Fragments arranged by size:
3278 1584 1210 160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2449 4293 5449 1790
Size: 659 1844 1156 2573
Fragments arranged by size:
2573 1844 1156 659
BmgBI CAC'GTC
Cuts at: 1318 2161 1318
Size: 843 5389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 3013 3963
Size: 416 53 83 399 24 636 960 950
Cuts at: 3963 4921 442
Size: 958 1753
Fragments arranged by size:
1753 960 958 950 636 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2357 1056
Size: 1301 4931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2583 4892 1239
Size: 1203 141 2309 2579
Fragments arranged by size:
2579 2309 1203 141
Bpu10I CC'TnA_GC
Cuts at: 2358 2358
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2803 4023 493
Size: 711 1599 1220 2702
Fragments arranged by size:
2702 1599 1220 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3046 4665 5063 5337 5484 5895 3046
Size: 1619 398 274 147 411 3383
Fragments arranged by size:
3383 1619 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3267 1186
Size: 2081 4151
BseYI C'CCAG_C
Cuts at: 2349 5435 5931 2349
Size: 3086 496 2650
Fragments arranged by size:
3086 2650 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 5308
Fragments arranged by size:
5308 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2383 4293 4378 5449 2383
Size: 1910 85 1071 3166
Fragments arranged by size:
3166 1910 1071 85
BsmI GAATG_Cn'
Cuts at: 2593 3743 3836 2593
Size: 1150 93 4989
Fragments arranged by size:
4989 1150 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2102 2102
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2383 2449 3551 4293 4378 5449 1391
Size: 399 593 66 1102 742 85 1071 2174
Fragments arranged by size:
2174 1102 1071 742 593 399 85 66
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 2946 4121 5851 2946
Size: 1175 1730 3327
Fragments arranged by size:
3327 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 3587 844
Size: 1164 1579 3489
Fragments arranged by size:
3489 1579 1164
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2994 4727 4901 65
Size: 1183 1746 1733 174 1396
Fragments arranged by size:
1746 1733 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3162 96
Size: 3066 3166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 794
Size: 1229 5003
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2688 2728 3187 3562 3929 4314 4331 5783
Size: 716 40 459 375 367 385 17 1452
Cuts at: 5783 5869 5881 6227 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 716 459 385 375 367 346
86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 3082 3082
Size: 6232
BtgI C'CryG_G
Cuts at: 513 1065 2724 513
Size: 552 1659 4021
Fragments arranged by size:
4021 1659 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4542 4562 3748
Size: 794 20 5418
Fragments arranged by size:
5418 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2423 5239 809
Size: 507 1107 2816 1802
Fragments arranged by size:
2816 1802 1107 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3679 4568
Size: 54 1425 50 275 18 378 1471 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1471 1425 889 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 4162 1353
Size: 801 2008 3423
Fragments arranged by size:
3423 2008 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 4819 5193 5339 387
Size: 1064 138 504 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 504 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2442 2583 2804 4295 4892 5679 5947
Size: 393 810 141 221 1491 597 787 268
Cuts at: 5947 1239
Size: 1524
Fragments arranged by size:
1524 1491 810 787 597 393 268 221
141
EcoO109I rG'GnC_Cy
Cuts at: 2055 2913 2055
Size: 858 5374
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 5379 1678
Size: 3701 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4873 4905 4947 4979 1373
Size: 32 135 32 3301 32 42 32 2626
Fragments arranged by size:
3301 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2875 3171 3576 3822 669
Size: 988 281 937 296 405 246 3079
Fragments arranged by size:
3079 988 937 405 296 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 2875 3822 2875
Size: 947 5285
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3283 5346 5530 1305
Size: 1978 2063 184 2007
Fragments arranged by size:
2063 2007 1978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 4032
Fragments arranged by size:
4032 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 4190 4549 4708 518
Size: 648 1782 1242 359 159 2042
Fragments arranged by size:
2042 1782 1242 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2312 2866 3071 3648 4325 5473 5718 1606
Size: 706 554 205 577 677 1148 245 2120
Fragments arranged by size:
2120 1148 706 677 577 554 245 205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5680
NdeI CA'TA_TG
Cuts at: 387 2399 387
Size: 2012 4220
NheI G'CTAG_C
Cuts at: 1052 2353 1052
Size: 1301 4931
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3169 3299 3590 5135 1132
Size: 97 1940 130 291 1545 2229
Fragments arranged by size:
2229 1940 1545 291 130 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 3165 3295 5131 3165
Size: 130 1836 4266
Fragments arranged by size:
4266 1836 130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PfoI T'CCnGG_A
Cuts at: 2532 2532
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2913 2055
Size: 858 5374
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 2217 3802 2217
Size: 1585 4647
PstI C_TGCA'G
Cuts at: 830 2118 2440 3070 3501 3584 830
Size: 1288 322 630 431 83 3478
Fragments arranged by size:
3478 1288 630 431 322 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2312 2866 3071 2312
Size: 554 205 5473
Fragments arranged by size:
5473 554 205
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 2523 4480 6192 2523
Size: 1957 1712 2563
Fragments arranged by size:
2563 1957 1712
SexAI A'CCwGG_T
Cuts at: 3400 3400
Size: 6232
SfcI C'TryA_G
Cuts at: 826 1045 2114 2436 3066 3497 3580 4715 5396
Size: 219 1069 322 630 431 83 1135 681
Cuts at: 5396 5587 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1069 681 630 431 322 219
191 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 3286 4156 5
Size: 47 3234 870 2081
Fragments arranged by size:
3234 2081 870 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 3671 513
Size: 552 2606 3074
Fragments arranged by size:
3074 2606 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2521 2770 3162
Size: 274 80 33 51 1443 544 249 392
Cuts at: 3162 4478 6190 96
Size: 1316 1712 138
Fragments arranged by size:
1712 1443 1316 544 392 274 249 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 1316
Size: 846 5386
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 2294 3261 4361 2294
Size: 967 1100 4165
Fragments arranged by size:
4165 1100 967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI