Restriction map for
pFN21AE0300
(Circular) (Six-base) MAPSORT of: pFN21AE0300.seq Check: 5224 from: 1 to: 9214
pFN21AE0300 9214 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 5563 6000 6198 2128
Size: 3435 437 198 5144
Fragments arranged by size:
5144 3435 437 198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7024 278
Size: 53 83 186 936 5488 2468
Fragments arranged by size:
5488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3264 6096 6557 1937
Size: 1327 2832 461 4594
Fragments arranged by size:
4594 2832 1327 461
Acc65I G'GTAC_C
Cuts at: 4447 4489 6535 4447
Size: 42 2046 7126
Fragments arranged by size:
7126 2046 42
AclI AA'CG_TT
Cuts at: 1563 7341 7714 1563
Size: 5778 373 3063
Fragments arranged by size:
5778 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2582 2690 3225 3311 4223 4290 4301
Size: 932 18 108 535 86 912 67 11
Cuts at: 4301 7277 8661 8929 1632
Size: 2976 1384 268 1917
Fragments arranged by size:
2976 1917 1384 932 912 535 268 108
86 67 18 11
AfeI AGC'GCT
Cuts at: 3714 5028 3714
Size: 1314 7900
AflII C'TTAA_G
Cuts at: 820 1017 4250 820
Size: 197 3233 5784
Fragments arranged by size:
5784 3233 197
AflIII A'CryG_T
Cuts at: 1203 3871 4532 8113 9090 1203
Size: 2668 661 3581 977 1327
Fragments arranged by size:
3581 2668 1327 977 661
AgeI A'CCGG_T
Cuts at: 8045 8045
Size: 9214
AhdI GACnn_n'nnGTC
Cuts at: 4951 7943 4951
Size: 2992 6222
AleI CACnn'nnGTG
Cuts at: 1166 5261 1166
Size: 4095 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2118 4439 5457 1184
Size: 934 2321 1018 4941
Fragments arranged by size:
4941 2321 1018 934
AlwNI CAG_nnn'CTG
Cuts at: 3418 3554 4589 4973 5607 6957 8529 3418
Size: 136 1035 384 634 1350 1572 4103
Fragments arranged by size:
4103 1572 1350 1035 634 384 136
ApaI G_GGCC'C
Cuts at: 3561 5765 6138 3561
Size: 2204 373 6637
Fragments arranged by size:
6637 2204 373
ApaLI G'TGCA_C
Cuts at: 3212 7271 8427 3212
Size: 4059 1156 3999
Fragments arranged by size:
4059 3999 1156
ApoI r'AATT_y
Cuts at: 1493 2237 4608 6523 6749 6901 9010 1493
Size: 744 2371 1915 226 152 2109 1697
Fragments arranged by size:
2371 2109 1915 1697 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2177 7768 160
Size: 872 1145 5591 1606
Fragments arranged by size:
5591 1606 1145 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9214
AvaI C'yCGr_G
Cuts at: 1870 1943 3887 5123 5147 5342 5365 5414 5429
Size: 73 1944 1236 24 195 23 49 15
Cuts at: 5429 5810 6041 6539 1870
Size: 381 231 498 4545
Fragments arranged by size:
4545 1944 1236 498 381 231 195 73
49 24 23 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4374 4407 4374
Size: 33 9181
BamHI G'GATC_C
Cuts at: 6544 6911 6544
Size: 367 8847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 4447 4489 5153 5988
Size: 325 217 627 326 2334 42 664 835
Cuts at: 5988 6400 6535 7990 618
Size: 412 135 1455 1842
Fragments arranged by size:
2334 1842 1455 835 664 627 412 326
325 217 135 42
BanII G_rGCy'C
Cuts at: 1391 3561 3896 5343 5765 6138 6533 1391
Size: 2170 335 1447 422 373 395 4072
Fragments arranged by size:
4072 2170 1447 422 395 373 335
BbeI G_GCGC'C
Cuts at: 2117 6404 2117
Size: 4287 4927
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 2311 4422 4806 5595 6504 928
Size: 971 393 19 2111 384 789 909 3638
Fragments arranged by size:
3638 2111 971 909 789 393 384 19
BbvCI CC'TCA_GC
Cuts at: 5921 5969 5921
Size: 48 9166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3335 3369 5693 5727 7405
Size: 34 50 34 1314 34 2324 34 1678
Cuts at: 7405 7439 1903
Size: 34 3678
Fragments arranged by size:
3678 2324 1678 1314 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2212 7106 8316 2212
Size: 4894 1210 3110
Fragments arranged by size:
4894 3110 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7825 136
Size: 107 122 71 631 605 6153 1525
Fragments arranged by size:
6153 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9209 9209
Size: 9214
BlpI GC'TnA_GC
Cuts at: 3447 6314 6631 3447
Size: 2867 317 6030
Fragments arranged by size:
6030 2867 317
Bme1580I G_kGCm'C
Cuts at: 1790 2483 3216 3561 5073 5156 5765 5961 6138
Size: 693 733 345 1512 83 609 196 177
Cuts at: 6138 7275 8431 1790
Size: 1137 1156 2573
Fragments arranged by size:
2573 1512 1156 1137 733 693 609 345
196 177 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4775 6945
Size: 416 53 83 399 24 2519 839 2170
Cuts at: 6945 7903 442
Size: 958 1753
Fragments arranged by size:
2519 2170 1753 958 839 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2919 3149 3627 4961 5024 5500 5629 7874
Size: 1680 230 478 1334 63 476 129 2245
Cuts at: 7874 1239
Size: 2579
Fragments arranged by size:
2579 2245 1680 1334 478 476 230 129
63
Bpu10I CC'TnA_GC
Cuts at: 3419 3779 5921 5969 6167 6203 3419
Size: 360 2142 48 198 36 6430
Fragments arranged by size:
6430 2142 360 198 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2379 3815 7339 8204 8502 2379
Size: 1436 3524 865 298 3091
Fragments arranged by size:
3524 3091 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3009 3187 4673 5936 6107 7877 882
Size: 617 1510 178 1486 1263 171 1770 2219
Fragments arranged by size:
2219 1770 1510 1486 1263 617 178 171
BsaAI yAC'GTr
Cuts at: 493 1204 3874 4533 7005 493
Size: 711 2670 659 2472 2702
Fragments arranged by size:
2702 2670 2472 711 659
BsaBI GATnn'nnATC
Cuts at: 9208 9208
Size: 9214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 6401 7021 7403
Size: 53 83 186 936 581 4287 620 382
Cuts at: 7403 8038 275
Size: 635 1451
Fragments arranged by size:
4287 1451 936 635 620 581 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3520 7647 8045 8319 8466 8877 3520
Size: 4127 398 274 147 411 3857
Fragments arranged by size:
4127 3857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8989
Fragments arranged by size:
8989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3307 3812 4691 4900 5372 5876 6159 1186
Size: 2121 505 879 209 472 504 283 4241
Fragments arranged by size:
4241 2121 879 505 504 472 283 209
BseYI C'CCAG_C
Cuts at: 2075 2308 2350 3123 3367 3765 4524 4966 5568
Size: 233 42 773 244 398 759 442 602
Cuts at: 5568 6130 6175 6181 8417 8913 2075
Size: 562 45 6 2236 496 2376
Fragments arranged by size:
2376 2236 773 759 602 562 496 442
398 244 233 45 42 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2182 2765 2774 4463 5656 1163
Size: 573 388 58 583 9 1689 1193 4721
Fragments arranged by size:
4721 1689 1193 583 573 388 58 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5665 6664 7425 8453 1540
Size: 275 186 3664 999 761 1028 2301
Fragments arranged by size:
3664 2301 1028 999 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 3216 4418 5952 6377 7275 7360 8431 2700
Size: 516 1202 1534 425 898 85 1071 3483
Fragments arranged by size:
3483 1534 1202 1071 898 516 425 85
BsmI GAATG_Cn'
Cuts at: 3332 4261 5626 6725 6818 3332
Size: 929 1365 1099 93 5728
Fragments arranged by size:
5728 1365 1099 929 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5267 5267
Size: 9214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2483 2700 3216 3561 3896 4418 5073
Size: 399 693 217 516 345 335 522 655
Cuts at: 5073 5156 5343 5765 5952 5961 6138 6377 6533
Size: 83 187 422 187 9 177 239 156
Cuts at: 6533 7275 7360 8431 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 655 522 516 422
399 345 335 239 217 187 187 177
156 85 83 9
BspEI T'CCGG_A
Cuts at: 8877 8877
Size: 9214
BspHI T'CATG_A
Cuts at: 7103 8833 7103
Size: 1730 7484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 2862 3240 4009 4435 5563 5904 5919
Size: 1284 734 378 769 426 1128 341 15
Cuts at: 5919 6000 6198 6318 6569 844
Size: 81 198 120 251 3489
Fragments arranged by size:
3489 1284 1128 769 734 426 378 341
251 198 120 81 15
BsrBI CCG'CTC
Cuts at: 1394 1823 5144 6171 7101 1394
Size: 429 3321 1027 930 3507
Fragments arranged by size:
3507 3321 1027 930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 6435 7709 7883 65
Size: 1183 3173 2014 1274 174 1396
Fragments arranged by size:
3173 2014 1396 1274 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2066 3363 4818 5083 5237 6364
Size: 110 207 49 1297 1455 265 154 1127
Cuts at: 6364 7858 8045 1700
Size: 1494 187 2869
Fragments arranged by size:
2869 1494 1455 1297 1127 265 207 187
154 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9214
BssSI C'ACGA_G
Cuts at: 3505 7274 8286 3505
Size: 3769 1012 4433
Fragments arranged by size:
4433 3769 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 2788 3220 5956 794
Size: 1985 9 432 2736 4052
Fragments arranged by size:
4052 2736 1985 432 9
BstBI TT'CG_AA
Cuts at: 4464 8018 4464
Size: 3554 5660
BstEII G'GTnAC_C
Cuts at: 1184 2663 1184
Size: 1479 7735
BstXI CCAn_nnnn'nTGG
Cuts at: 5524 6130 5524
Size: 606 8608
BstYI r'GATC_y
Cuts at: 1972 3550 5045 6280 6544 6911 7296 7313 8765
Size: 1578 1495 1235 264 367 385 17 1452
Cuts at: 8765 8851 8863 9209 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1578 1495 1452 1235 385 367 346
264 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3596 6187 3596
Size: 2591 6623
BtgI C'CryG_G
Cuts at: 513 1065 2754 3198 5178 5583 5685 513
Size: 552 1689 444 1980 405 102 4042
Fragments arranged by size:
4042 1980 1689 552 444 405 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2938 4799 5199 5256 5655 487
Size: 683 56 1712 1861 400 57 399 4046
Fragments arranged by size:
4046 1861 1712 683 400 399 57 56
BtsI GCAGTG_nn'
Cuts at: 2077 3284 6035 6730 7524 7544 2077
Size: 1207 2751 695 794 20 3747
Fragments arranged by size:
3747 2751 1207 794 695 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9179
DraI TTT'AAA
Cuts at: 3997 4854 6518 3997
Size: 857 1664 6693
Fragments arranged by size:
6693 1664 857
DraIII CAC_nnn'GTG
Cuts at: 5956 5956
Size: 9214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3260 8221 809
Size: 507 1944 4961 1802
Fragments arranged by size:
4961 1944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 3580 5191
Size: 54 1425 50 275 18 933 817 1611
Cuts at: 5191 6018 6389 6661 7550 9179 8
Size: 827 371 272 889 1629 43
Fragments arranged by size:
1629 1611 1425 933 889 827 817 371
275 272 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6661 1537
Size: 275 4849 4090
Fragments arranged by size:
4849 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 7144 1353
Size: 5791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 5371 6345 6501 7801 8175
Size: 1064 138 455 3327 974 156 1300 374
Cuts at: 8175 8321 387
Size: 146 1280
Fragments arranged by size:
3327 1300 1280 1064 974 455 374 156
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2582 2690 2919 3149 3225 3311
Size: 393 932 18 108 229 230 76 86
Cuts at: 3311 3627 4223 4290 4301 4961 5024 5500 5629
Size: 316 596 67 11 660 63 476 129
Cuts at: 5629 7277 7874 8661 8929 1239
Size: 1648 597 787 268 1524
Fragments arranged by size:
1648 1524 932 787 660 597 596 476
393 316 268 230 229 129 108 86
76 67 63 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 4940 5837 4940
Size: 897 8317
EcoO109I rG'GnC_Cy
Cuts at: 2215 3415 3601 4650 5362 5761 5893 6134 6135
Size: 1200 186 1049 712 399 132 241 1
Cuts at: 6135 2215
Size: 5294
Fragments arranged by size:
5294 1200 1049 712 399 241 186 132
1
EcoRI G'AATT_C
Cuts at: 6523 6901 6523
Size: 378 8836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2800 2476
Size: 324 8890
FspI TGC'GCA
Cuts at: 2684 7720 2684
Size: 5036 4178
HaeII r_GCGC'y
Cuts at: 1678 2117 3716 3779 5030 6404 8361 1678
Size: 439 1599 63 1251 1374 1957 2531
Fragments arranged by size:
2531 1957 1599 1374 1251 439 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 4634 4666 4840
Size: 32 135 32 707 32 2323 32 174
Cuts at: 4840 4872 4937 4969 5122 5154 5318 5336 5350
Size: 32 65 32 153 32 164 18 14
Cuts at: 5350 5368 6257 6289 7855 7887 7929 7961 1373
Size: 18 889 32 1566 32 42 32 2626
Fragments arranged by size:
2626 2323 1566 889 707 174 164 153
135 65 42 32 32 32 32 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 6558 6804 669
Size: 988 281 1327 3293 246 3079
Fragments arranged by size:
3293 3079 1327 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9214
HpaI GTT'AAC
Cuts at: 6804 6804
Size: 9214
KasI G'GCGC_C
Cuts at: 2113 6400 2113
Size: 4287 4927
KpnI G_GTAC'C
Cuts at: 4451 4493 6539 4451
Size: 42 2046 7126
Fragments arranged by size:
7126 2046 42
MfeI C'AATT_G
Cuts at: 6813 6813
Size: 9214
MluI A'CGCG_T
Cuts at: 9090 9090
Size: 9214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2587 2853 3090 3345 3625 5913 8328 8512
Size: 1282 266 237 255 280 2288 2415 184
Cuts at: 8512 1305
Size: 2007
Fragments arranged by size:
2415 2288 2007 1282 280 266 255 237
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7392
Fragments arranged by size:
7392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4230 5261 7172 7531 7690 518
Size: 648 3064 1031 1911 359 159 2042
Fragments arranged by size:
3064 2042 1911 1031 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3127 3200 3250 3579 5199 5285 5637
Size: 1150 371 73 50 329 1620 86 352
Cuts at: 5637 6017 6630 7307 8455 8700 1606
Size: 380 613 677 1148 245 2120
Fragments arranged by size:
2120 1620 1150 1148 677 613 380 371
352 329 245 86 73 50
NaeI GCC'GGC
Cuts at: 2019 2068 4820 5085 5239 2019
Size: 49 2752 265 154 5994
Fragments arranged by size:
5994 2752 265 154 49
NarI GG'CG_CC
Cuts at: 2114 6401 2114
Size: 4287 4927
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8662
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9214
NgoMIV G'CCGG_C
Cuts at: 2017 2066 4818 5083 5237 2017
Size: 49 2752 265 154 5994
Fragments arranged by size:
5994 2752 265 154 49
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9214
NotI GC'GGCC_GC
Cuts at: 6661 6661
Size: 9214
NspI r_CATG'y
Cuts at: 1132 1229 2704 4345 6572 8117 1132
Size: 97 1475 1641 2227 1545 2229
Fragments arranged by size:
2229 2227 1641 1545 1475 97
PasI CC'CwG_GG
Cuts at: 1348 5293 1348
Size: 3945 5269
PciI A'CATG_T
Cuts at: 8113 8113
Size: 9214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4639 1108
Size: 3531 5683
PfoI T'CCnGG_A
Cuts at: 4551 4758 4938 4551
Size: 207 180 8827
Fragments arranged by size:
8827 207 180
PmeI GTTT'AAAC
Cuts at: 6518 6518
Size: 9214
PmlI CAC'GTG
Cuts at: 3874 4533 3874
Size: 659 8555
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7283 7315 8796 8828 7283
Size: 32 1481 32 7669
Fragments arranged by size:
7669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 3601 4650 5362 5893 2215
Size: 1386 1049 712 531 5536
Fragments arranged by size:
5536 1386 1049 712 531
PshAI GACnn'nnGTC
Cuts at: 1613 4578 1613
Size: 2965 6249
PsiI TTA'TAA
Cuts at: 6784 6784
Size: 9214
PspOMI G'GGCC_C
Cuts at: 3557 5761 6134 3557
Size: 2204 373 6637
Fragments arranged by size:
6637 2204 373
PstI C_TGCA'G
Cuts at: 830 2128 2733 2977 3243 6566 830
Size: 1298 605 244 266 3323 3478
Fragments arranged by size:
3478 3323 1298 605 266 244
PvuI CG_AT'CG
Cuts at: 2001 5665 2001
Size: 3664 5550
PvuII CAG'CTG
Cuts at: 3127 5285 3127
Size: 2158 7056
SacII CC_GC'GG
Cuts at: 2757 3201 2757
Size: 444 8770
SalI G'TCGA_C
Cuts at: 1936 3263 6556 1936
Size: 1327 3293 4594
Fragments arranged by size:
4594 3293 1327
SanDI GG'GwC_CC
Cuts at: 3601 3601
Size: 9214
SbfI CC_TGCA'GG
Cuts at: 6566 6566
Size: 9214
ScaI AGT'ACT
Cuts at: 6347 7462 9174 6347
Size: 1115 1712 6387
Fragments arranged by size:
6387 1712 1115
SfcI C'TryA_G
Cuts at: 826 1045 2124 2376 2729 2973 3239 6562 7697
Size: 219 1079 252 353 244 266 3323 1135
Cuts at: 7697 8378 8569 826
Size: 681 191 1471
Fragments arranged by size:
3323 1471 1135 1079 681 353 266 252
244 219 191
SfoI GGC'GCC
Cuts at: 2115 6402 2115
Size: 4287 4927
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9214
SgrAI Cr'CCGG_yG
Cuts at: 1700 2017 1700
Size: 317 8897
SmaI CCC'GGG
Cuts at: 1872 5367 6541 1872
Size: 3495 1174 4545
Fragments arranged by size:
4545 3495 1174
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2394 3794 4250 7318 8219 8481
Size: 197 926 451 1400 456 3068 901 262
Cuts at: 8481 820
Size: 1553
Fragments arranged by size:
3068 1553 1400 926 901 456 451 262
197
SnaBI TAC'GTA
Cuts at: 493 7005 493
Size: 6512 2702
SpeI A'CTAG_T
Cuts at: 152 5065 152
Size: 4913 4301
SphI G_CATG'C
Cuts at: 1132 6572 1132
Size: 5440 3774
SspI AAT'ATT
Cuts at: 5 52 2175 2341 2593 3957 7138 5
Size: 47 2123 166 252 1364 3181 2081
Fragments arranged by size:
3181 2123 2081 1364 252 166 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9214
StyI C'CwwG_G
Cuts at: 513 1065 5385 6653 513
Size: 552 4320 1268 3074
Fragments arranged by size:
4320 3074 1268 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 2999 3416 5960 6479 7241 7258
Size: 1255 1071 348 417 2544 519 762 17
Cuts at: 7258 7411 7596 8041 325
Size: 153 185 445 1498
Fragments arranged by size:
2544 1498 1255 1071 762 519 445 417
348 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 3833 6345
Size: 274 80 33 51 1443 1829 27 2512
Cuts at: 6345 7460 9172 96
Size: 1115 1712 138
Fragments arranged by size:
2512 1829 1712 1443 1115 274 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9174 9206 9174
Size: 32 9182
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9214
XbaI T'CTAG_A
Cuts at: 6550 6550
Size: 9214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3364 4661 5728 3364
Size: 1297 1067 6850
Fragments arranged by size:
6850 1297 1067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9214
XmaI C'CCGG_G
Cuts at: 1870 5365 6539 1870
Size: 3495 1174 4545
Fragments arranged by size:
4545 3495 1174
XmnI GAAnn'nnTTC
Cuts at: 2625 7343 2625
Size: 4718 4496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7022 276
Size: 53 83 186 936 5488 2468
Fragments arranged by size:
5488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacI SapI SexAI SfiI SrfI SwaI