Restriction map for
pFN21AE0299
(Circular) (Six-base) MAPSORT of: pFN21AE0299.seq Check: 847 from: 1 to: 20134
pFN21AE0299 20134 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3235 4981 5392 3235
Size: 1746 411 17977
Fragments arranged by size:
17977 1746 411
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6883 17944 278
Size: 53 83 186 936 5347 11061 2468
Fragments arranged by size:
11061 5347 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4984 10748 13103 14509 17477 1937
Size: 3047 5764 2355 1406 2968 4594
Fragments arranged by size:
5764 4594 3047 2968 2355 1406
Acc65I G'GTAC_C
Cuts at: 17455 17455
Size: 20134
AclI AA'CG_TT
Cuts at: 1563 18261 18634 1563
Size: 16698 373 3063
Fragments arranged by size:
16698 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2634 2678 4007 4137 4386 4804 5604 5670
Size: 1002 44 1329 130 249 418 800 66
Cuts at: 5670 5691 5712 5754 5841 6696 6741 7149 7308
Size: 21 21 42 87 855 45 408 159
Cuts at: 7308 8496 8868 9633 9891 10041 11070 11076 11265
Size: 1188 372 765 258 150 1029 6 189
Cuts at: 11265 11604 11643 12069 12084 14991 15102 15225 17031
Size: 339 39 426 15 2907 111 123 1806
Cuts at: 17031 17276 18197 19581 19849 1632
Size: 245 921 1384 268 1917
Fragments arranged by size:
2907 1917 1806 1384 1329 1188 1029 1002
921 855 800 765 426 418 408 372
339 268 258 249 245 189 159 150
130 123 111 87 66 45 44 42
39 21 21 15 6
AfeI AGC'GCT
Cuts at: 11696 11696
Size: 20134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 19937
AflIII A'CryG_T
Cuts at: 1203 7893 13506 16282 19033 20010 1203
Size: 6690 5613 2776 2751 977 1327
Fragments arranged by size:
6690 5613 2776 2751 1327 977
AgeI A'CCGG_T
Cuts at: 18965 18965
Size: 20134
AhdI GACnn_n'nnGTC
Cuts at: 9105 11136 18863 9105
Size: 2031 7727 10376
Fragments arranged by size:
10376 7727 2031
AleI CACnn'nnGTG
Cuts at: 1166 11348 1166
Size: 10182 9952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 20134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4206 4238 11971 12003 15939 15971 4206
Size: 32 7733 32 3936 32 8369
Fragments arranged by size:
8369 7733 3936 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2069 2200 2549 3692 4400 4973 5018 6719 8861
Size: 131 349 1143 708 573 45 1701 2142
Cuts at: 8861 11012 11174 11477 11926 12518 12674 15794 15811
Size: 2151 162 303 449 592 156 3120 17
Cuts at: 15811 16748 17009 17877 19449 2069
Size: 937 261 868 1572 2754
Fragments arranged by size:
3120 2754 2151 2142 1701 1572 1143 937
868 708 592 573 449 349 303 261
162 156 131 45 17
ApaI G_GGCC'C
Cuts at: 4891 4891
Size: 20134
ApaLI G'TGCA_C
Cuts at: 18191 19347 18191
Size: 1156 18978
ApoI r'AATT_y
Cuts at: 1493 2519 4834 5556 6132 8460 8660 8679 9637
Size: 1026 2315 722 576 2328 200 19 958
Cuts at: 9637 9894 10999 11730 13595 14399 14612 14768 15076
Size: 257 1105 731 1865 804 213 156 308
Cuts at: 15076 15687 15799 16098 17094 17123 17443 17669 17821
Size: 611 112 299 996 29 320 226 152
Cuts at: 17821 19930 1493
Size: 2109 1697
Fragments arranged by size:
2328 2315 2109 1865 1697 1105 1026 996
958 804 731 722 611 576 320 308
299 257 226 213 200 156 152 112
29 19
AseI AT'TA_AT
Cuts at: 160 1032 18688 160
Size: 872 17656 1606
Fragments arranged by size:
17656 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20134
AvaI C'yCGr_G
Cuts at: 1870 1943 7237 15240 15577 16170 16849 17459 1870
Size: 73 5294 8003 337 593 679 610 4545
Fragments arranged by size:
8003 5294 4545 679 610 593 337 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7188 7221 8133 8166 10350 10383 12405 12438 7188
Size: 33 912 33 2184 33 2022 33 14884
Fragments arranged by size:
14884 2184 2022 912 33 33 33 33
BamHI G'GATC_C
Cuts at: 2686 5218 14356 15980 17464 17831 2686
Size: 2532 9138 1624 1484 367 4989
Fragments arranged by size:
9138 4989 2532 1624 1484 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4093 17455 18910 618
Size: 325 217 627 1514 792 13362 1455 1842
Fragments arranged by size:
13362 1842 1514 1455 792 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2099 3940 4891 5034 7020 7453 7557
Size: 649 59 1841 951 143 1986 433 104
Cuts at: 7557 10506 11667 13093 13156 13923 17453 1391
Size: 2949 1161 1426 63 767 3530 4072
Fragments arranged by size:
4072 3530 2949 1986 1841 1426 1161 951
767 649 433 143 104 63 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 3995 5742 6642 6883 6906 7896
Size: 971 403 1693 1747 900 241 23 990
Cuts at: 7896 9699 14298 14349 17072 928
Size: 1803 4599 51 2723 3990
Fragments arranged by size:
4599 3990 2723 1803 1747 1693 990 971
900 403 241 51 23
BbvCI CC'TCA_GC
Cuts at: 3622 7746 9555 11703 13491 3622
Size: 4124 1809 2148 1788 10265
Fragments arranged by size:
10265 4124 2148 1809 1788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3633 3667 11924 11958 15013 15047 18325
Size: 34 1696 34 8257 34 3055 34 3278
Cuts at: 18325 18359 1903
Size: 34 3678
Fragments arranged by size:
8257 3678 3278 3055 1696 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10509 10678 12185 12406 16485 18026 19236 10509
Size: 169 1507 221 4079 1541 1210 11407
Fragments arranged by size:
11407 4079 1541 1507 1210 221 169
BclI T'GATC_A
Cuts at: 1548 5992 8782 9025 14122 16504 17137 17199 1548
Size: 4444 2790 243 5097 2382 633 62 4483
Fragments arranged by size:
5097 4483 4444 2790 2382 633 243 62
BfrBI ATG'CAT
Cuts at: 2837 7511 7771 14999 2837
Size: 4674 260 7228 7972
Fragments arranged by size:
7972 7228 4674 260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 3013 18745
Size: 107 122 71 631 605 394 947 15732
Cuts at: 18745 136
Size: 1525
Fragments arranged by size:
15732 1525 947 631 605 394 122 107
71
BglII A'GATC_T
Cuts at: 7562 9838 11077 11949 20129 7562
Size: 2276 1239 872 8180 7567
Fragments arranged by size:
8180 7567 2276 1239 872
BlpI GC'TnA_GC
Cuts at: 2147 3003 3093 3111 3123 3180 3490 3610 3657
Size: 856 90 18 12 57 310 120 47
Cuts at: 3657 5118 10500 11868 13395 14824 17551 2147
Size: 1461 5382 1368 1527 1429 2727 4730
Fragments arranged by size:
5382 4730 2727 1527 1461 1429 1368 856
310 120 90 57 47 18 12
Bme1580I G_kGCm'C
Cuts at: 1790 4891 12631 13241 14804 18195 19351 1790
Size: 3101 7740 610 1563 3391 1156 2573
Fragments arranged by size:
7740 3391 3101 2573 1563 1156 610
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 20134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3053 3141 4506
Size: 416 53 83 399 24 1636 88 1365
Cuts at: 4506 4797 8897 8937 11224 11833 14807 17865 18823
Size: 291 4100 40 2287 609 2974 3058 958
Cuts at: 18823 442
Size: 1753
Fragments arranged by size:
4100 3058 2974 2287 1753 1636 1365 958
609 416 399 291 88 83 53 40
24
BmtI G_CTAG'C
Cuts at: 1056 2085 1056
Size: 1029 19105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 12498 13493 2118
Size: 10380 995 8759
Fragments arranged by size:
10380 8759 995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2742 2931 3072 3102 3189 3210 3276
Size: 852 651 189 141 30 87 21 66
Cuts at: 3276 3306 3942 5171 7002 8018 9114 9330 9951
Size: 30 636 1229 1831 1016 1096 216 621
Cuts at: 9951 10086 10566 11696 11948 12273 12532 13170 13280
Size: 135 480 1130 252 325 259 638 110
Cuts at: 13280 13463 14686 14869 15875 16388 18794 1239
Size: 183 1223 183 1006 513 2406 2579
Fragments arranged by size:
2579 2406 1831 1229 1223 1130 1096 1016
1006 852 651 638 636 621 513 480
325 259 252 216 189 183 183 141
135 110 87 66 30 30 21
Bpu10I CC'TnA_GC
Cuts at: 2664 3297 3622 4098 7746 9468 9513 9555 11703
Size: 633 325 476 3648 1722 45 42 2148
Cuts at: 11703 12179 13491 15870 2664
Size: 476 1312 2379 6928
Fragments arranged by size:
6928 3648 2379 2148 1722 1312 633 476
476 325 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4758 5790 6034 6155 6255 11906 13806 14059 14256
Size: 1032 244 121 100 5651 1900 253 197
Cuts at: 14256 14946 16337 18259 19124 19422 4758
Size: 690 1391 1922 865 298 5470
Fragments arranged by size:
5651 5470 1922 1900 1391 1032 865 690
298 253 244 197 121 100
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 4772 9092 9386 10079 14274 14631
Size: 617 837 2436 4320 294 693 4195 357
Cuts at: 14631 18797 882
Size: 4166 2219
Fragments arranged by size:
4320 4195 4166 2436 2219 837 693 617
357 294
BsaAI yAC'GTr
Cuts at: 493 1204 7896 17925 493
Size: 711 6692 10029 2702
Fragments arranged by size:
10029 6692 2702 711
BsaBI GATnn'nnATC
Cuts at: 12438 14121 16399 20128 12438
Size: 1683 2278 3729 12444
Fragments arranged by size:
12444 3729 2278 1683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2314 6880 17941 18323
Size: 53 83 186 936 781 4566 11061 382
Cuts at: 18323 18958 275
Size: 635 1451
Fragments arranged by size:
11061 4566 1451 936 781 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 18567 18965 19239 19386 19797 18567
Size: 398 274 147 411 18904
Fragments arranged by size:
18904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2779 2809 2868 2898 3070
Size: 30 165 30 1376 30 59 30 172
Cuts at: 3070 3100 3304 3334 3394 3424 3706 3736 3738
Size: 30 204 30 60 30 282 30 2
Cuts at: 3738 3768 4366 4396 9436 9466 10084 10114 10612
Size: 30 598 30 5040 30 618 30 498
Cuts at: 10612 10642 11774 11804 11971 12001 12246 12276 16131
Size: 30 1132 30 167 30 245 30 3855
Cuts at: 16131 16161 16789 16819 1178
Size: 30 628 30 4493
Fragments arranged by size:
5040 4493 3855 1376 1132 628 618 598
498 282 245 204 172 167 165 60
59 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2088 2091 2171 2176 2401 2787 4374 4761
Size: 902 3 80 5 225 386 1587 387
Cuts at: 4761 6416 6674 6999 7002 7565 7763 9210 9219
Size: 1655 258 325 3 563 198 1447 9
Cuts at: 9219 9234 9237 9240 9243 9261 9444 11795 11945
Size: 15 3 3 3 18 183 2351 150
Cuts at: 11945 13211 16837 1186
Size: 1266 3626 4483
Fragments arranged by size:
4483 3626 2351 1655 1587 1447 1266 902
563 387 386 325 258 225 198 183
150 80 18 15 9 5 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2998 3240 3387 3430 3460 3550 3640 3975 5011
Size: 242 147 43 30 90 90 335 1036
Cuts at: 5011 11174 13003 15804 15995 19337 19833 2998
Size: 6163 1829 2801 191 3342 496 3299
Fragments arranged by size:
6163 3342 3299 2801 1829 1036 496 335
242 191 147 90 90 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3978 4053 5370 9432 10470 10849 1163
Size: 573 2242 75 1317 4062 1038 379 10448
Fragments arranged by size:
10448 4062 2242 1317 1038 573 379 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2121 2226 17584 18345 19373
Size: 275 186 60 60 105 15358 761 1028
Cuts at: 19373 1540
Size: 2301
Fragments arranged by size:
15358 2301 1028 761 275 186 105 60
60
BsiHKAI G_wGCw'C
Cuts at: 2448 3940 7020 7453 7557 11608 11667 12185 18195
Size: 1492 3080 433 104 4051 59 518 6010
Cuts at: 18195 18280 19351 2448
Size: 85 1071 3231
Fragments arranged by size:
6010 4051 3231 3080 1492 1071 518 433
104 85 59
BsmI GAATG_Cn'
Cuts at: 5252 8310 11044 15263 16255 16788 17645 17738 5252
Size: 3058 2734 4219 992 533 857 93 7648
Fragments arranged by size:
7648 4219 3058 2734 992 857 533 93
BsmBI CGTCTCn'nnnn_
Cuts at: 8352 11528 11852 14246 8352
Size: 3176 324 2394 14240
Fragments arranged by size:
14240 3176 2394 324
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2099 2448 3940 4891 5034 7020
Size: 399 250 59 349 1492 951 143 1986
Cuts at: 7020 7453 7557 10506 11608 11667 12185 12631 13093
Size: 433 104 2949 1102 59 518 446 462
Cuts at: 13093 13156 13241 13923 14804 17453 18195 18280 19351
Size: 63 85 682 881 2649 742 85 1071
Cuts at: 19351 1391
Size: 2174
Fragments arranged by size:
2949 2649 2174 1986 1492 1102 1071 951
881 742 682 518 462 446 433 399
349 250 143 104 85 85 63 59
59
BspEI T'CCGG_A
Cuts at: 19797 19797
Size: 20134
BspHI T'CATG_A
Cuts at: 11844 14212 15820 18023 19753 11844
Size: 2368 1608 2203 1730 12225
Fragments arranged by size:
12225 2368 2203 1730 1608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3235 4483 4981 5392 9127 11407 14859 16600
Size: 2391 1248 498 411 3735 2280 3452 1741
Cuts at: 16600 17489 844
Size: 889 3489
Fragments arranged by size:
3735 3489 3452 2391 2280 1741 1248 889
498 411
BsrBI CCG'CTC
Cuts at: 1394 1823 11830 18021 1394
Size: 429 10007 6191 3507
Fragments arranged by size:
10007 6191 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 9755 10467 11466 12159 15197 18629 18803
Size: 1183 8507 712 999 693 3038 3432 174
Cuts at: 18803 65
Size: 1396
Fragments arranged by size:
8507 3432 3038 1396 1183 999 712 693
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 14265 16815 16841 18778 18965 1700
Size: 110 310 12145 2550 26 1937 187 2869
Fragments arranged by size:
12145 2869 2550 1937 310 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 12340 96
Size: 12244 7890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 20134
BssSI C'ACGA_G
Cuts at: 12483 12630 13281 18194 19206 12483
Size: 147 651 4913 1012 13411
Fragments arranged by size:
13411 4913 1012 651 147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 2377 5247 8224 10457 16748 794
Size: 1406 177 2870 2977 2233 6291 4180
Fragments arranged by size:
6291 4180 2977 2870 2233 1406 177
BstBI TT'CG_AA
Cuts at: 6673 13348 14248 18938 6673
Size: 6675 900 4690 7869
Fragments arranged by size:
7869 6675 4690 900
BstEII G'GTnAC_C
Cuts at: 1184 4626 5569 1184
Size: 3442 943 15749
Fragments arranged by size:
15749 3442 943
BstXI CCAn_nnnn'nTGG
Cuts at: 3395 5149 9841 13276 3395
Size: 1754 4692 3435 10253
Fragments arranged by size:
10253 4692 3435 1754
BstYI r'GATC_y
Cuts at: 1972 2686 3689 3949 4081 5218 7151 7562 8008
Size: 714 1003 260 132 1137 1933 411 446
Cuts at: 8008 8240 9838 10699 11077 11892 11949 13257 14221
Size: 232 1598 861 378 815 57 1308 964
Cuts at: 14221 14356 14777 15947 15980 17374 17464 17831 18216
Size: 135 421 1170 33 1394 90 367 385
Cuts at: 18216 18233 19685 19771 19783 20129 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1933 1598 1452 1394 1308 1170 1137
1003 964 861 815 714 446 421 411
385 378 367 346 260 232 135 132
90 86 57 33 17 12
BstZ17I GTA'TAC
Cuts at: 4985 13104 4985
Size: 8119 12015
Bsu36I CC'TnA_GG
Cuts at: 3010 4176 4698 8079 10896 13194 3010
Size: 1166 522 3381 2817 2298 9950
Fragments arranged by size:
9950 3381 2817 2298 1166 522
BtgI C'CryG_G
Cuts at: 513 1065 2004 8289 13895 16341 16576 513
Size: 552 939 6285 5606 2446 235 4071
Fragments arranged by size:
6285 5606 4071 2446 939 552 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 19395
Fragments arranged by size:
19395 683 56
BtsI GCAGTG_nn'
Cuts at: 2894 4023 4845 5457 6622 8374 10599 11391 16873
Size: 1129 822 612 1165 1752 2225 792 5482
Cuts at: 16873 16902 17650 18444 18464 2894
Size: 29 748 794 20 4564
Fragments arranged by size:
5482 4564 2225 1752 1165 1129 822 794
792 748 612 29 20
ClaI AT'CG_AT
Cuts at: 1554 10939 1554
Size: 9385 10749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4282 4317 15819 15854 529
Size: 35 3718 35 11502 35 4809
Fragments arranged by size:
11502 4809 3718 35 35 35
DraI TTT'AAA
Cuts at: 2436 5325 6442 7006 12319 17438 2436
Size: 2889 1117 564 5313 5119 5132
Fragments arranged by size:
5313 5132 5119 2889 1117 564
DraIII CAC_nnn'GTG
Cuts at: 3840 4877 6128 3840
Size: 1037 1251 17846
Fragments arranged by size:
17846 1251 1037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 14266 15426 19141 809
Size: 507 12950 1160 3715 1802
Fragments arranged by size:
12950 3715 1802 1160 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2118 2223
Size: 54 1425 50 275 18 228 60 105
Cuts at: 2223 4660 10374 15109 15126 17581 18470 20099 8
Size: 2437 5714 4735 17 2455 889 1629 43
Fragments arranged by size:
5714 4735 2455 2437 1629 1425 889 275
228 105 60 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2118 2223 17581 1537
Size: 275 246 60 105 15358 4090
Fragments arranged by size:
15358 4090 275 246 105 60
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2436 4362 5586 5631 6393 6517 6540
Size: 795 288 1926 1224 45 762 124 23
Cuts at: 6540 6651 7083 7518 7963 8207 8599 9165 9979
Size: 111 432 435 445 244 392 566 814
Cuts at: 9979 10810 10896 11820 13684 13695 14947 17295 18064
Size: 831 86 924 1864 11 1252 2348 769
Cuts at: 18064 1353
Size: 3423
Fragments arranged by size:
3423 2348 1926 1864 1252 1224 924 831
814 795 769 762 566 445 435 432
392 288 244 124 111 86 45 23
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 14351 18721 19095 19241 387
Size: 1064 138 558 12204 4370 374 146 1280
Fragments arranged by size:
12204 4370 1280 1064 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 3938 7018 7451 7555 11665 3938
Size: 3080 433 104 4110 12407
Fragments arranged by size:
12407 4110 3080 433 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2634 2678 2742 2931 3072 3102
Size: 393 459 543 44 64 189 141 30
Cuts at: 3102 3189 3210 3276 3306 3942 4007 4137 4386
Size: 87 21 66 30 636 65 130 249
Cuts at: 4386 4804 5171 5604 5670 5691 5712 5754 5841
Size: 418 367 433 66 21 21 42 87
Cuts at: 5841 6696 6741 7002 7149 7308 8018 8496 8868
Size: 855 45 261 147 159 710 478 372
Cuts at: 8868 9114 9330 9633 9891 9951 10041 10086 10566
Size: 246 216 303 258 60 90 45 480
Cuts at: 10566 11070 11076 11265 11604 11643 11696 11948 12069
Size: 504 6 189 339 39 53 252 121
Cuts at: 12069 12084 12273 12532 13170 13280 13463 14686 14869
Size: 15 189 259 638 110 183 1223 183
Cuts at: 14869 14991 15102 15225 15875 16388 17031 17276 18197
Size: 122 111 123 650 513 643 245 921
Cuts at: 18197 18794 19581 19849 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1223 921 855 787 710 650 643
638 636 597 543 513 504 480 478
459 433 418 393 372 367 339 303
268 261 259 258 252 249 246 245
216 189 189 189 183 183 159 147
141 130 123 122 121 111 110 90
87 87 66 66 65 64 60 53
45 45 44 42 39 30 30 21
21 21 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 3339 3747 13445 16418 2477
Size: 862 408 9698 2973 6193
Fragments arranged by size:
9698 6193 2973 862 408
EcoO109I rG'GnC_Cy
Cuts at: 2675 2783 3368 4243 4887 4888 5396 8257 8899
Size: 108 585 875 644 1 508 2861 642
Cuts at: 8899 11225 13438 15965 16014 2675
Size: 2326 2213 2527 49 6795
Fragments arranged by size:
6795 2861 2527 2326 2213 875 644 642
585 508 108 49 1
EcoRI G'AATT_C
Cuts at: 13595 15076 15687 17443 17821 13595
Size: 1481 611 1756 378 15908
Fragments arranged by size:
15908 1756 1481 611 378
EcoRV GAT'ATC
Cuts at: 2541 11488 12579 2541
Size: 8947 1091 10096
Fragments arranged by size:
10096 8947 1091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5333 5846 6228 6546 7460 8757 9086 9213 13382
Size: 513 382 318 914 1297 329 127 4169
Cuts at: 13382 14949 16229 5333
Size: 1567 1280 9238
Fragments arranged by size:
9238 4169 1567 1297 1280 914 513 382
329 318 127
FspI TGC'GCA
Cuts at: 18640 18640
Size: 20134
HaeII r_GCGC'y
Cuts at: 1678 11698 19281 1678
Size: 10020 7583 2531
Fragments arranged by size:
10020 7583 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2525 2557 3193
Size: 32 135 32 863 32 58 32 636
Cuts at: 3193 3225 3340 3372 4175 4207 4289 4321 4513
Size: 32 115 32 803 32 82 32 192
Cuts at: 4513 4545 4800 4832 5090 5122 6023 6055 6098
Size: 32 255 32 258 32 901 32 43
Cuts at: 6098 6130 6281 6313 7685 7717 8798 8830 9091
Size: 32 151 32 1372 32 1081 32 261
Cuts at: 9091 9123 11122 11154 11774 11806 11851 11883 12246
Size: 32 1999 32 620 32 45 32 363
Cuts at: 12246 12278 12289 12321 13493 13525 14410 14442 15759
Size: 32 11 32 1172 32 885 32 1317
Cuts at: 15759 15791 16218 16250 18775 18807 18849 18881 1373
Size: 32 427 32 2525 32 42 32 2626
Fragments arranged by size:
2626 2525 1999 1372 1317 1172 1081 901
885 863 803 636 620 427 363 261
258 255 192 151 135 115 82 58
45 43 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 4543 9333 15431 17395 17478
Size: 988 281 547 2058 4790 6098 1964 83
Cuts at: 17478 17724 669
Size: 246 3079
Fragments arranged by size:
6098 4790 3079 2058 1964 988 547 281
246 83
HindIII A'AGCT_T
Cuts at: 748 5616 5828 7791 8059 8958 14372 15650 748
Size: 4868 212 1963 268 899 5414 1278 5232
Fragments arranged by size:
5414 5232 4868 1963 1278 899 268 212
HpaI GTT'AAC
Cuts at: 2485 9333 17724 2485
Size: 6848 8391 4895
Fragments arranged by size:
8391 6848 4895
KpnI G_GTAC'C
Cuts at: 17459 17459
Size: 20134
MfeI C'AATT_G
Cuts at: 7011 12099 15063 17733 7011
Size: 5088 2964 2670 9412
Fragments arranged by size:
9412 5088 2964 2670
MluI A'CGCG_T
Cuts at: 20010 20010
Size: 20134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 3175 3260 5075 6173 7541 8961 9587
Size: 939 931 85 1815 1098 1368 1420 626
Cuts at: 9587 9723 9803 10342 10501 11318 11757 11920 12580
Size: 136 80 539 159 817 439 163 660
Cuts at: 12580 13091 13673 15307 16428 19248 19432 1305
Size: 511 582 1634 1121 2820 184 2007
Fragments arranged by size:
2820 2007 1815 1634 1420 1368 1121 1098
939 931 817 660 626 582 539 511
439 184 163 159 136 85 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4662 10376 15111 15128 10
Size: 54 1425 343 2830 5714 4735 17 5016
Fragments arranged by size:
5714 5016 4735 2830 1425 343 54 17
MslI CAynn'nnrTG
Cuts at: 518 1166 6689 11348 14325 18092 18451 18610 518
Size: 648 5523 4659 2977 3767 359 159 2042
Fragments arranged by size:
5523 4659 3767 2977 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2146 2177 2228 4658 5018 5117 7670
Size: 460 80 31 51 2430 360 99 2553
Cuts at: 7670 7802 9401 9887 11902 11926 12089 12841 14186
Size: 132 1599 486 2015 24 163 752 1345
Cuts at: 14186 15140 15919 17550 18227 19375 19620 1606
Size: 954 779 1631 677 1148 245 2120
Fragments arranged by size:
2553 2430 2120 2015 1631 1599 1345 1148
954 779 752 677 486 460 360 245
163 132 99 80 51 31 24
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 20134
NcoI C'CATG_G
Cuts at: 513 1065 2004 8289 16341 16576 513
Size: 552 939 6285 8052 235 4071
Fragments arranged by size:
8052 6285 4071 939 552 235
NdeI CA'TA_TG
Cuts at: 387 10951 15709 387
Size: 10564 4758 4812
Fragments arranged by size:
10564 4812 4758
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 20134
NheI G'CTAG_C
Cuts at: 1052 2081 1052
Size: 1029 19105
NotI GC'GGCC_GC
Cuts at: 2058 17581 2058
Size: 15523 4611
NsiI A_TGCA'T
Cuts at: 2839 7513 7773 15001 2839
Size: 4674 260 7228 7972
Fragments arranged by size:
7972 7228 4674 260
NspI r_CATG'y
Cuts at: 1132 1229 3806 3834 7169 10404 10460 10499 10592
Size: 97 2577 28 3335 3235 56 39 93
Cuts at: 10592 13510 15290 16286 16609 17435 17492 19037 1132
Size: 2918 1780 996 323 826 57 1545 2229
Fragments arranged by size:
3335 3235 2918 2577 2229 1780 1545 996
826 323 97 93 57 56 39 28
PasI CC'CwG_GG
Cuts at: 1348 2309 10672 15351 15857 16001 1348
Size: 961 8363 4679 506 144 5481
Fragments arranged by size:
8363 5481 4679 961 506 144
PciI A'CATG_T
Cuts at: 13506 16282 19033 13506
Size: 2776 2751 14607
Fragments arranged by size:
14607 2776 2751
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2936 3247 8765 11926 13798 15118 15811 1108
Size: 1828 311 5518 3161 1872 1320 693 5431
Fragments arranged by size:
5518 5431 3161 1872 1828 1320 693 311
PfoI T'CCnGG_A
Cuts at: 2935 3187 11667 13247 13296 2935
Size: 252 8480 1580 49 9773
Fragments arranged by size:
9773 8480 1580 252 49
PmeI GTTT'AAAC
Cuts at: 17438 17438
Size: 20134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11971 12003 18203 18235 19716 19748 11971
Size: 32 6200 32 1481 32 12357
Fragments arranged by size:
12357 6200 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2675 2783 3368 5396 8899 11225 15965 16014 2675
Size: 108 585 2028 3503 2326 4740 49 6795
Fragments arranged by size:
6795 4740 3503 2326 2028 585 108 49
PshAI GACnn'nnGTC
Cuts at: 1613 10394 1613
Size: 8781 11353
PsiI TTA'TAA
Cuts at: 7656 9660 10331 10842 11441 16329 17704 7656
Size: 2004 671 511 599 4888 1375 10086
Fragments arranged by size:
10086 4888 2004 1375 671 599 511
PspOMI G'GGCC_C
Cuts at: 4887 4887
Size: 20134
PspXI vC'TCGA_Gb
Cuts at: 15240 15240
Size: 20134
PstI C_TGCA'G
Cuts at: 830 3708 4852 7051 7543 11790 13265 14101 14835
Size: 2878 1144 2199 492 4247 1475 836 734
Cuts at: 14835 15139 15166 16825 16909 17281 17486 830
Size: 304 27 1659 84 372 205 3478
Fragments arranged by size:
4247 3478 2878 2199 1659 1475 1144 836
734 492 372 304 205 84 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 20134
PvuII CAG'CTG
Cuts at: 2146 5018 5117 7670 7802 9401 9887 11902 11926
Size: 2872 99 2553 132 1599 486 2015 24
Cuts at: 11926 12089 12841 15140 2146
Size: 163 752 2299 7140
Fragments arranged by size:
7140 2872 2553 2299 2015 1599 752 486
163 132 99 24
SacI G_AGCT'C
Cuts at: 3940 7020 7453 7557 11667 3940
Size: 3080 433 104 4110 12407
Fragments arranged by size:
12407 4110 3080 433 104
SalI G'TCGA_C
Cuts at: 1936 17476 1936
Size: 15540 4594
SanDI GG'GwC_CC
Cuts at: 16014 16014
Size: 20134
SapI GCTCTTCn'nnn_
Cuts at: 2436 5631 7083 8599 10810 11820 13695 14947 2436
Size: 3195 1452 1516 2211 1010 1875 1252 7623
Fragments arranged by size:
7623 3195 2211 1875 1516 1452 1252 1010
SbfI CC_TGCA'GG
Cuts at: 17281 17486 17281
Size: 205 19929
ScaI AGT'ACT
Cuts at: 6769 12975 13676 18382 20094 6769
Size: 6206 701 4706 1712 6809
Fragments arranged by size:
6809 6206 4706 1712 701
SexAI A'CCwGG_T
Cuts at: 2326 2677 10527 12169 2326
Size: 351 7850 1642 10291
Fragments arranged by size:
10291 7850 1642 351
SfcI C'TryA_G
Cuts at: 826 1045 2349 3284 3704 3856 4848 5424 7047
Size: 219 1304 935 420 152 992 576 1623
Cuts at: 7047 7539 8157 8832 9354 9867 9888 10425 10539
Size: 492 618 675 522 513 21 537 114
Cuts at: 10539 11100 11133 11786 12000 13261 14097 14470 14831
Size: 561 33 653 214 1261 836 373 361
Cuts at: 14831 15135 15162 15905 16821 16905 17277 17482 18617
Size: 304 27 743 916 84 372 205 1135
Cuts at: 18617 19298 19489 826
Size: 681 191 1471
Fragments arranged by size:
1623 1471 1304 1261 1135 992 935 916
836 743 681 675 653 618 576 561
537 522 513 492 420 373 372 361
304 219 214 205 191 152 114 84
33 27 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20134
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 20134
SmaI CCC'GGG
Cuts at: 1872 15579 17461 1872
Size: 13707 1882 4545
Fragments arranged by size:
13707 4545 1882
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4773 5805 6049 6170 6270 11885
Size: 197 926 2830 1032 244 121 100 5615
Cuts at: 11885 13821 14038 14271 14961 15240 16170 16352 18238
Size: 1936 217 233 690 279 930 182 1886
Cuts at: 18238 19139 19401 820
Size: 901 262 1553
Fragments arranged by size:
5615 2830 1936 1886 1553 1032 930 926
901 690 279 262 244 233 217 197
182 121 100
SnaBI TAC'GTA
Cuts at: 493 17925 493
Size: 17432 2702
SpeI A'CTAG_T
Cuts at: 152 4120 6596 13639 152
Size: 3968 2476 7043 6647
Fragments arranged by size:
7043 6647 3968 2476
SphI G_CATG'C
Cuts at: 1132 7169 10499 17492 1132
Size: 6037 3330 6993 3774
Fragments arranged by size:
6993 6037 3774 3330
SspI AAT'ATT
Cuts at: 5 52 7043 7580 14752 17113 17179 18058 5
Size: 47 6991 537 7172 2361 66 879 2081
Fragments arranged by size:
7172 6991 2361 2081 879 537 66 47
StuI AGG'CCT
Cuts at: 1517 2845 3753 9511 9609 1517
Size: 1328 908 5758 98 12042
Fragments arranged by size:
12042 5758 1328 908 98
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 4089 4158 6076 6378 6643
Size: 552 939 201 1884 69 1918 302 265
Cuts at: 6643 8289 9819 12118 12642 16341 16414 16466 16576
Size: 1646 1530 2299 524 3699 73 52 110
Cuts at: 16576 17573 513
Size: 997 3074
Fragments arranged by size:
3699 3074 2299 1918 1884 1646 1530 997
939 552 524 302 265 201 110 73
69 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 4957 8563 8614 9045 10436 12781
Size: 1255 1085 2292 3606 51 431 1391 2345
Cuts at: 12781 12871 12898 13720 15402 15551 18161 18178 18331
Size: 90 27 822 1682 149 2610 17 153
Cuts at: 18331 18516 18961 325
Size: 185 445 1498
Fragments arranged by size:
3606 2610 2345 2292 1682 1498 1391 1255
1085 822 445 431 185 153 149 90
51 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4351 5809 6245
Size: 274 80 33 51 1443 2374 1458 436
Cuts at: 6245 6278 6767 7025 7642 9440 10055 10412 10706
Size: 33 489 258 617 1798 615 357 294
Cuts at: 10706 12156 12340 12835 12973 13674 14022 16263 18380
Size: 1450 184 495 138 701 348 2241 2117
Cuts at: 18380 20092 96
Size: 1712 138
Fragments arranged by size:
2374 2241 2117 1798 1712 1458 1450 1443
701 617 615 495 489 436 357 348
294 274 258 184 138 138 80 51
33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3738 3770 8367 8399 10507 10539 10612 10644 11699
Size: 32 4597 32 2108 32 73 32 1055
Cuts at: 11699 11731 12159 12191 12469 12484 12501 12516 12721
Size: 32 428 32 278 15 17 15 205
Cuts at: 12721 12753 20094 20126 3738
Size: 32 7341 32 3746
Fragments arranged by size:
7341 4597 3746 2108 1055 428 278 205
73 32 32 32 32 32 32 32
32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 3133 8281 10529 15378 1316
Size: 1817 5148 2248 4849 6072
Fragments arranged by size:
6072 5148 4849 2248 1817
XbaI T'CTAG_A
Cuts at: 9388 10117 14312 14977 15416 17470 9388
Size: 729 4195 665 439 2054 12052
Fragments arranged by size:
12052 4195 2054 729 665 439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3689 8261 8608 9817 12838 16640 3689
Size: 4572 347 1209 3021 3802 7183
Fragments arranged by size:
7183 4572 3802 3021 1209 347
XhoI C'TCGA_G
Cuts at: 1943 15240 16170 1943
Size: 13297 930 5907
Fragments arranged by size:
13297 5907 930
XmaI C'CCGG_G
Cuts at: 1870 15577 17459 1870
Size: 13707 1882 4545
Fragments arranged by size:
13707 4545 1882
XmnI GAAnn'nnTTC
Cuts at: 3379 5556 7408 9193 11577 11983 13947 15717 17256
Size: 2177 1852 1785 2384 406 1964 1770 1539
Cuts at: 17256 18263 3379
Size: 1007 5250
Fragments arranged by size:
5250 2384 2177 1964 1852 1785 1770 1539
1007 406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6881 17942 276
Size: 53 83 186 936 5347 11061 2468
Fragments arranged by size:
11061 5347 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BsiWI FseI FspAI KasI NarI
NruI PacI PmlI PsrI RsrII SacII SfiI SfoI
SrfI SwaI