Restriction map for
pFN21AE0291
(Circular) (Six-base) MAPSORT of: pFN21AE0291.seq Check: 9552 from: 1 to: 5926
pFN21AE0291 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2494 3269 1937
Size: 557 775 4594
Fragments arranged by size:
4594 775 557
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2690 3989 5373 5641 1632
Size: 545 513 1299 1384 268 1917
Fragments arranged by size:
1917 1384 1299 545 513 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2843 4825 5802 1203
Size: 1640 1982 977 1327
Fragments arranged by size:
1982 1640 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 1184
Size: 1234 4692
AlwNI CAG_nnn'CTG
Cuts at: 3114 3669 5241 3114
Size: 555 1572 3799
Fragments arranged by size:
3799 1572 555
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2739 4480 160
Size: 872 1707 1741 1606
Fragments arranged by size:
1741 1707 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 3251 1870
Size: 73 1308 4545
Fragments arranged by size:
4545 1308 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2822 3247 4702 618
Size: 325 217 627 1035 425 1455 1842
Fragments arranged by size:
1842 1455 1035 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2221 2262 2349 2538 2583 3245 1391
Size: 774 56 41 87 189 45 662 4072
Fragments arranged by size:
4072 774 662 189 87 56 45 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2984 928
Size: 971 1085 3870
Fragments arranged by size:
3870 1085 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 2328 3139 1548
Size: 780 811 4335
Fragments arranged by size:
4335 811 780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5921 5921
Size: 5926
BlpI GC'TnA_GC
Cuts at: 2240 3343 2240
Size: 1103 4823
Bme1580I G_kGCm'C
Cuts at: 1790 3987 5143 1790
Size: 2197 1156 2573
Fragments arranged by size:
2573 2197 1156
BmgBI CAC'GTC
Cuts at: 1318 2844 1318
Size: 1526 4400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2343 2439 3657
Size: 416 53 83 399 24 926 96 1218
Cuts at: 3657 4615 442
Size: 958 1753
Fragments arranged by size:
1753 1218 958 926 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2363 2748 4586 1239
Size: 830 294 385 1838 2579
Fragments arranged by size:
2579 1838 830 385 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2546 4589 882
Size: 617 1047 2043 2219
Fragments arranged by size:
2219 2043 1047 617
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 2330 5920 2330
Size: 3590 2336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2837 3733 4115 4750
Size: 53 83 186 936 1304 896 382 635
Cuts at: 4750 275
Size: 1451
Fragments arranged by size:
1451 1304 936 896 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2575 4359 4757 5031 5178 5589 2575
Size: 1784 398 274 147 411 2912
Fragments arranged by size:
2912 1784 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3114 1186
Size: 1928 3998
BseYI C'CCAG_C
Cuts at: 2582 5129 5625 2582
Size: 2547 496 2883
Fragments arranged by size:
2883 2547 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2167 2619 1163
Size: 573 431 452 4470
Fragments arranged by size:
4470 573 452 431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 2538 3987 4072 5143 2027
Size: 511 1449 85 1071 2810
Fragments arranged by size:
2810 1449 1071 511 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2194 2194
Size: 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2165 2221 2262 2349 2538 2583
Size: 399 237 138 56 41 87 189 45
Cuts at: 2583 3245 3987 4072 5143 1391
Size: 662 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 399 237 189 138
87 85 56 45 41
BspEI T'CCGG_A
Cuts at: 2575 5589 2575
Size: 3014 2912
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2638 2783 4421 4595 65
Size: 1183 1390 145 1638 174 1396
Fragments arranged by size:
1638 1396 1390 1183 174 145
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2901 794
Size: 2107 3819
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2726 2726
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2044 2399 3256 3623 4008 4025 5477 5563
Size: 72 355 857 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 857 385 367 355 346 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 513
Size: 552 939 249 4186
Fragments arranged by size:
4186 939 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 487
Size: 683 56 866 4321
Fragments arranged by size:
4321 866 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2607 3442 4236 4256 2100
Size: 507 835 794 20 3770
Fragments arranged by size:
3770 835 794 507 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DraIII CAC_nnn'GTG
Cuts at: 2341 2341
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3189 3373 4262
Size: 54 1425 50 275 18 1359 184 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1359 889 275 184 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2657 3856 1353
Size: 1304 1199 3423
Fragments arranged by size:
3423 1304 1199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 2536
Size: 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2177 2363 2690 2748 3989 4586
Size: 393 437 108 186 327 58 1241 597
Cuts at: 4586 5373 5641 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1241 787 597 437 393 327 268
186 108 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2290 2290
Size: 5926
EcoO109I rG'GnC_Cy
Cuts at: 2135 2135
Size: 5926
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 5073 1678
Size: 3395 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 2698 2730 2833
Size: 32 135 32 789 32 305 32 103
Cuts at: 2833 2865 4567 4599 4641 4673 1373
Size: 32 1702 32 42 32 2626
Fragments arranged by size:
2626 1702 789 305 135 103 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2785 5040 5224 1305
Size: 1480 2255 184 2007
Fragments arranged by size:
2255 2007 1480 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3191 10
Size: 54 1425 343 1359 2745
Fragments arranged by size:
2745 1425 1359 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 3884 4243 4402 518
Size: 648 1068 1650 359 159 2042
Fragments arranged by size:
2042 1650 1068 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 3133 3342 4019 5167 5412 1606
Size: 460 1067 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 460 245 209
NcoI C'CATG_G
Cuts at: 513 1065 2004 2253 513
Size: 552 939 249 4186
Fragments arranged by size:
4186 939 552 249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3284 4829 1132
Size: 97 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2194 1348
Size: 846 5080
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2341 2425 1108
Size: 1233 84 4609
Fragments arranged by size:
4609 1233 84
PfoI T'CCnGG_A
Cuts at: 2517 2517
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 3995 4027 5508 5540 2303
Size: 32 1660 32 1481 32 2689
Fragments arranged by size:
2689 1660 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SacI G_AGCT'C
Cuts at: 2538 2538
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SapI GCTCTTCn'nnn_
Cuts at: 2657 2657
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2923 3219 3274 4409 5090 5281 826
Size: 219 1878 296 55 1135 681 191 1471
Fragments arranged by size:
1878 1471 1135 681 296 219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 2743 3850 5
Size: 47 2691 1107 2081
Fragments arranged by size:
2691 2081 1107 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 3365 513
Size: 552 939 249 1112 3074
Fragments arranged by size:
3074 1112 939 552 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2528 3166 3953 3970 4123 4308 4753
Size: 1255 948 638 787 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 948 787 638 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2846 4172 5884
Size: 274 80 33 51 1443 869 1326 1712
Cuts at: 5884 96
Size: 138
Fragments arranged by size:
1712 1443 1326 869 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI