Restriction map for
pFN21AE0288
(Circular) (Six-base) MAPSORT of: pFN21AE0288.seq Check: 4374 from: 1 to: 5644
pFN21AE0288 5644 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2610 2987 1937
Size: 673 377 4594
Fragments arranged by size:
4594 673 377
Acc65I G'GTAC_C
Cuts at: 2373 2965 2373
Size: 592 5052
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2395 3387 4959 2395
Size: 992 1572 3080
Fragments arranged by size:
3080 1572 992
ApaI G_GGCC'C
Cuts at: 2520 2520
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2052 2953 3179 3331 5440 1493
Size: 559 901 226 152 2109 1697
Fragments arranged by size:
2109 1697 901 559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2462 2974 3341 2462
Size: 512 367 4765
Fragments arranged by size:
4765 512 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2373 2709 2965 4420 618
Size: 325 217 627 586 336 256 1455 1842
Fragments arranged by size:
1842 1455 627 586 336 325 256 217
BanII G_rGCy'C
Cuts at: 1391 2479 2520 2737 2963 1391
Size: 1088 41 217 226 4072
Fragments arranged by size:
4072 1088 226 217 41
BbeI G_GCGC'C
Cuts at: 2713 2713
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2070 3536 4746 2070
Size: 1466 1210 2968
Fragments arranged by size:
2968 1466 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2456 4255 136
Size: 107 122 71 631 605 784 1799 1525
Fragments arranged by size:
1799 1525 784 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 2421 2426 3061 2421
Size: 5 635 5004
Fragments arranged by size:
5004 635 5
Bme1580I G_kGCm'C
Cuts at: 1790 2520 2769 3705 4861 1790
Size: 730 249 936 1156 2573
Fragments arranged by size:
2573 1156 936 730 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2332 3375 4333
Size: 416 53 83 399 24 915 1043 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1043 958 915 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2466 4304 1239
Size: 1226 1 1838 2579
Fragments arranged by size:
2579 1838 1226 1
Bpu10I CC'TnA_GC
Cuts at: 2072 2072
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2710 3451 3833 4468
Size: 53 83 186 936 1177 741 382 635
Cuts at: 4468 275
Size: 1451
Fragments arranged by size:
1451 1177 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2724 2754 1178
Size: 30 165 30 1321 30 4068
Fragments arranged by size:
4068 1321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 2642 1186
Size: 1032 424 4188
Fragments arranged by size:
4188 1032 424
BseYI C'CCAG_C
Cuts at: 2122 4847 5343 2122
Size: 2725 496 2423
Fragments arranged by size:
2725 2423 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 3705 3790 4861 2412
Size: 1293 85 1071 3195
Fragments arranged by size:
3195 1293 1071 85
BsmI GAATG_Cn'
Cuts at: 2916 3155 3248 2916
Size: 239 93 5312
Fragments arranged by size:
5312 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2479 2520 2737 2769 2963 3705
Size: 399 622 67 41 217 32 194 742
Cuts at: 3705 3790 4861 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 622 399 217 194 85
67 41 32
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 2250 2412 3533 5263 2250
Size: 162 1121 1730 2631
Fragments arranged by size:
2631 1730 1121 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2999 844
Size: 1295 860 3489
Fragments arranged by size:
3489 1295 860
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 96
Size: 2088 3556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2340 794
Size: 1546 4098
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 2749 1184
Size: 1565 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 2527 2527
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2462 2974 3341 3726 3743 5195 5281 5293
Size: 490 512 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 512 490 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2786 2786
Size: 5644
BtgI C'CryG_G
Cuts at: 513 1065 2004 2470 2745 2835 513
Size: 552 939 466 275 90 3322
Fragments arranged by size:
3322 939 552 466 275 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 3160 3954 3974 2562
Size: 598 794 20 4232
Fragments arranged by size:
4232 794 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DraIII CAC_nnn'GTG
Cuts at: 2340 2340
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 4651 809
Size: 507 1269 2066 1802
Fragments arranged by size:
2066 1802 1269 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2838 3091 3980
Size: 54 1425 50 275 18 1008 253 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1008 889 275 253 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 4231 4605 4751 387
Size: 1064 138 900 1742 374 146 1280
Fragments arranged by size:
1742 1280 1064 900 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2466 3707 4304 5091 5359 1239
Size: 393 833 1 1241 597 787 268 1524
Fragments arranged by size:
1524 1241 833 787 597 393 268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2368 2368
Size: 5644
EcoRI G'AATT_C
Cuts at: 2052 2953 3331 2052
Size: 901 378 4365
Fragments arranged by size:
4365 901 378
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2433 2713 4791 1678
Size: 755 280 2078 2531
Fragments arranged by size:
2531 2078 755 280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373
Size: 32 135 32 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 2590 2988 3234 669
Size: 988 281 93 559 398 246 3079
Fragments arranged by size:
3079 988 559 398 281 246 93
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2709 2709
Size: 5644
KpnI G_GTAC'C
Cuts at: 2377 2969 2377
Size: 592 5052
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2357 2618 3602 3961 4120 518
Size: 648 1050 141 261 984 359 159 2042
Fragments arranged by size:
2042 1050 984 648 359 261 159 141
MspA1I CmG'CkG
Cuts at: 1606 2122 2699 2822 3060 3737 4885 5130 1606
Size: 516 577 123 238 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 516 245 238 123
NarI GG'CG_CC
Cuts at: 2710 2710
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2004 2470 513
Size: 552 939 466 3687
Fragments arranged by size:
3687 939 552 466
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2291 1108
Size: 1183 4461
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2516 2516
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2557 2996 830
Size: 1727 439 3478
Fragments arranged by size:
3478 1727 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2122 2699 2822 2122
Size: 577 123 4944
Fragments arranged by size:
4944 577 123
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2553 2992 4127 4808 4999 826
Size: 219 1508 439 1135 681 191 1471
Fragments arranged by size:
1508 1471 1135 681 439 219 191
SfoI GGC'GCC
Cuts at: 2711 2711
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3748 4649 4911 820
Size: 197 926 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 2791 1517
Size: 1274 4370
StyI C'CwwG_G
Cuts at: 513 1065 2004 2162 2470 3083 513
Size: 552 939 158 308 613 3074
Fragments arranged by size:
3074 939 613 552 308 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 3671 3688 3841 4026 4471 325
Size: 1255 771 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2757 3890
Size: 274 80 33 51 1443 207 573 1133
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1133 573 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2699 2806 2699
Size: 107 5537
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI