Restriction map for
pFN21AE0284
(Circular) (Six-base) MAPSORT of: pFN21AE0284.seq Check: 1203 from: 1 to: 5716
pFN21AE0284 5716 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2549 3059 1937
Size: 612 510 4594
Fragments arranged by size:
4594 612 510
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 3779 5163 5431 1632
Size: 522 1625 1384 268 1917
Fragments arranged by size:
1917 1625 1384 522 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2559 4615 5592 1203
Size: 1356 2056 977 1327
Fragments arranged by size:
2056 1356 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaI G_GGCC'C
Cuts at: 2117 2117
Size: 5716
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2823 4270 160
Size: 872 1791 1447 1606
Fragments arranged by size:
1791 1606 1447 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2135 3041 1870
Size: 73 173 19 906 4545
Fragments arranged by size:
4545 906 173 73 19
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3037 4492 618
Size: 325 217 627 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2117 2315 2613 3035 1391
Size: 726 198 298 422 4072
Fragments arranged by size:
4072 726 422 298 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2972 928
Size: 971 1073 3672
Fragments arranged by size:
3672 1073 971
BbvCI CC'TCA_GC
Cuts at: 2897 2897
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2117 3777 4933 1790
Size: 327 1660 1156 2573
Fragments arranged by size:
2573 1660 1156 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2755 3447 4405
Size: 416 53 83 399 24 1338 692 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1338 958 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2246 1056
Size: 1190 4526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 2687 4376 1239
Size: 1041 407 1689 2579
Fragments arranged by size:
2579 1689 1041 407
Bpu10I CC'TnA_GC
Cuts at: 2897 2897
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 4379 882
Size: 617 572 2308 2219
Fragments arranged by size:
2308 2219 617 572
BsaAI yAC'GTr
Cuts at: 493 1204 2466 3507 493
Size: 711 1262 1041 2702
Fragments arranged by size:
2702 1262 1041 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2341 4149 4547 4821 4968 5379 2341
Size: 1808 398 274 147 411 2678
Fragments arranged by size:
2678 1808 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 2049 2079 2531
Size: 30 165 30 599 30 17 30 452
Cuts at: 2531 2561 1178
Size: 30 4333
Fragments arranged by size:
4333 599 452 165 30 30 30 30
30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5716
BseYI C'CCAG_C
Cuts at: 2179 2308 4919 5415 2179
Size: 129 2611 496 2480
Fragments arranged by size:
2611 2480 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 3777 3862 4933 2613
Size: 1164 85 1071 3396
Fragments arranged by size:
3396 1164 1071 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2315 2613 3035 3777 3862 4933
Size: 399 327 198 298 422 742 85 1071
Cuts at: 4933 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 422 399 327 298 198
85
BspEI T'CCGG_A
Cuts at: 2341 5379 2341
Size: 3038 2678
BspHI T'CATG_A
Cuts at: 2961 3605 5335 2961
Size: 644 1730 3342
Fragments arranged by size:
3342 1730 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 2949 4211 4385 65
Size: 1183 1164 537 1262 174 1396
Fragments arranged by size:
1396 1262 1183 1164 537 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 2916 4360 4547 1700
Size: 110 376 730 1444 187 2869
Fragments arranged by size:
2869 1444 730 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 2603 96
Size: 2507 3209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2662 794
Size: 1868 3848
BstBI TT'CG_AA
Cuts at: 2202 4520 2202
Size: 2318 3398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2371 2387 2371
Size: 16 5700
BstYI r'GATC_y
Cuts at: 1972 2707 3046 3413 3798 3815 5267 5353 5365
Size: 735 339 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 735 385 367 346 339 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2490 2490
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 2004 2380 2397 2535 2631 2850 513
Size: 552 939 376 17 138 96 219 3379
Fragments arranged by size:
3379 939 552 376 219 138 96 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2822 3232 4026 4046 2822
Size: 410 794 20 4492
Fragments arranged by size:
4492 794 410 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 4723 809
Size: 507 1440 1967 1802
Fragments arranged by size:
1967 1802 1440 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 3163 4052
Size: 54 1425 50 275 18 697 636 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 697 636 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3646 1353
Size: 2293 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2816 4303 4677 4823 387
Size: 1064 138 1227 1487 374 146 1280
Fragments arranged by size:
1487 1280 1227 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2280 2687 3779 4376 5163 5431
Size: 393 522 126 407 1092 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 1092 787 597 522 407 393 268
126
EcoO109I rG'GnC_Cy
Cuts at: 2893 2893
Size: 5716
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FseI GG_CCGG'CC
Cuts at: 2920 2920
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2049 2081 2473
Size: 32 135 32 430 32 15 32 392
Cuts at: 2473 2505 2531 2563 2973 3005 4357 4389 4431
Size: 32 26 32 410 32 1352 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1352 430 410 392 135 42 32
32 32 32 32 32 32 32 32
26 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2562 3674 4033 4192 518
Size: 648 1396 1112 359 159 2042
Fragments arranged by size:
2042 1396 1112 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2183 2928 3132 3809 4957 5202 1606
Size: 418 159 745 204 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 677 418 245 204 159
NaeI GCC'GGC
Cuts at: 2918 2918
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 2004 2397 2535 2631 2850 513
Size: 552 939 393 138 96 219 3379
Fragments arranged by size:
3379 939 552 393 219 138 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2916 2916
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 2242 1052
Size: 1190 4526
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2563 3074 4619 1132
Size: 97 1334 511 1545 2229
Fragments arranged by size:
2229 1545 1334 511 97
PasI CC'CwG_GG
Cuts at: 1348 2049 1348
Size: 701 5015
PciI A'CATG_T
Cuts at: 2559 4615 2559
Size: 2056 3660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 1108
Size: 1677 4039
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PmlI CAC'GTG
Cuts at: 2466 2466
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2113 2113
Size: 5716
PstI C_TGCA'G
Cuts at: 830 2658 3068 830
Size: 1828 410 3478
Fragments arranged by size:
3478 1828 410
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2183 2183
Size: 5716
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2783 3964 5676 2783
Size: 1181 1712 2823
Fragments arranged by size:
2823 1712 1181
SfcI C'TryA_G
Cuts at: 826 1045 2574 2654 3064 4199 4880 5071 826
Size: 219 1529 80 410 1135 681 191 1471
Fragments arranged by size:
1529 1471 1135 681 410 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 2137 3043 1872
Size: 265 906 4545
Fragments arranged by size:
4545 906 265
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 2700 1517
Size: 1183 4533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 2535 2631 2850 3155 513
Size: 552 939 393 138 96 219 305 3074
Fragments arranged by size:
3074 939 552 393 305 219 138 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3743 3760 3913 4098 4543 325
Size: 1255 2163 17 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2603 2781 3962
Size: 274 80 33 51 1443 626 178 1181
Cuts at: 3962 5674 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1181 626 274 178 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 2878 3052 2878
Size: 174 5542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 2404 2368
Size: 36 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 2135 3041 1870
Size: 265 906 4545
Fragments arranged by size:
4545 906 265
XmnI GAAnn'nnTTC
Cuts at: 3011 3845 3011
Size: 834 4882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI