Restriction map for
pFN21AE0279
(Circular) (Six-base) MAPSORT of: pFN21AE0279.seq Check: 2977 from: 1 to: 5329
pFN21AE0279 5329 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3139 278
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 2496 2672 1937
Size: 340 219 176 4594
Fragments arranged by size:
4594 340 219 176
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3392 4776 5044 1632
Size: 1760 1384 268 1917
Fragments arranged by size:
1917 1760 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 4228 5205 1203
Size: 3025 977 1327
Fragments arranged by size:
3025 1327 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 2353 4058 2353
Size: 1705 3624
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5329
AlwNI CAG_nnn'CTG
Cuts at: 3072 4644 3072
Size: 1572 3757
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2222 2638 2864 3016 5125 1493
Size: 729 416 226 152 2109 1697
Fragments arranged by size:
2109 1697 729 416 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 1870
Size: 73 711 4545
Fragments arranged by size:
4545 711 73
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2600 2650 4105 618
Size: 325 217 627 813 50 1455 1842
Fragments arranged by size:
1842 1455 813 627 325 217 50
BanII G_rGCy'C
Cuts at: 1391 2648 1391
Size: 1257 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 928
Size: 971 252 4106
Fragments arranged by size:
4106 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3520 3554 1903
Size: 34 1583 34 3678
Fragments arranged by size:
3678 1583 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 4431 3221
Size: 1210 4119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3940 136
Size: 107 122 71 631 605 2268 1525
Fragments arranged by size:
2268 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2112 5324 2112
Size: 3212 2117
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 2089 3390 4546 1790
Size: 299 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5329
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 3060 4018
Size: 416 53 83 399 24 602 1041 958
Cuts at: 4018 442
Size: 1753
Fragments arranged by size:
1753 1041 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3989 1239
Size: 2750 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2422 3454 4319 4617 2422
Size: 1032 865 298 3134
Fragments arranged by size:
3134 1032 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2580 3992 882
Size: 617 1081 1412 2219
Fragments arranged by size:
2219 1412 1081 617
BsaAI yAC'GTr
Cuts at: 493 1204 2311 3120 493
Size: 711 1107 809 2702
Fragments arranged by size:
2702 1107 809 711
BsaBI GATnn'nnATC
Cuts at: 5323 5323
Size: 5329
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3136 3518 4153 275
Size: 53 83 186 936 1603 382 635 1451
Fragments arranged by size:
1603 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2542 3762 4160 4434 4581 4992 2542
Size: 1220 398 274 147 411 2879
Fragments arranged by size:
2879 1220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5104
Fragments arranged by size:
5104 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5329
BseYI C'CCAG_C
Cuts at: 2210 4532 5028 2210
Size: 2322 496 2511
Fragments arranged by size:
2511 2322 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4756
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2779 3540 4568 1540
Size: 275 186 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2310 3390 3475 4546 2310
Size: 1080 85 1071 3093
Fragments arranged by size:
3093 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2310 2648 3390 3475 4546 1391
Size: 399 299 221 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 299 221 85
BspEI T'CCGG_A
Cuts at: 2542 4992 2542
Size: 2450 2879
BspHI T'CATG_A
Cuts at: 3218 4948 3218
Size: 1730 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 844
Size: 1840 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 1394
Size: 429 1393 3507
Fragments arranged by size:
3507 1393 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2621 3824 3998 65
Size: 1183 1373 1203 174 1396
Fragments arranged by size:
1396 1373 1203 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3973 4160 1700
Size: 110 2163 187 2869
Fragments arranged by size:
2869 2163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5329
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5329
BssSI C'ACGA_G
Cuts at: 2372 3389 4401 2372
Size: 1017 1012 3300
Fragments arranged by size:
3300 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 794
Size: 1454 3875
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2112 2659 3026 3411 3428 4880 4966 4978
Size: 140 547 367 385 17 1452 86 12
Cuts at: 4978 5324 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 547 385 367 346 140 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4590
Fragments arranged by size:
4590 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 3639 3659 2845
Size: 794 20 4515
Fragments arranged by size:
4515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2776 3665
Size: 54 1425 50 275 18 546 400 889
Cuts at: 3665 5294 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 546 400 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2776 1537
Size: 275 964 4090
Fragments arranged by size:
4090 964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3259 1353
Size: 1906 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3916 4290 4436 387
Size: 1064 138 2327 374 146 1280
Fragments arranged by size:
2327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3392 3989 4776 5044 1239
Size: 393 1760 597 787 268 1524
Fragments arranged by size:
1760 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2479 2479
Size: 5329
EcoRI G'AATT_C
Cuts at: 2222 2638 3016 2222
Size: 416 378 4535
Fragments arranged by size:
4535 416 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 5329
FspI TGC'GCA
Cuts at: 2252 3835 2252
Size: 1583 3746
FspAI rTGC'GCAy
Cuts at: 2252 2252
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 2019 2232 4476 1678
Size: 341 213 2244 2531
Fragments arranged by size:
2531 2244 341 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2339 2371 3970
Size: 32 135 32 589 32 146 32 1599
Cuts at: 3970 4002 4044 4076 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1599 589 146 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2497 2673 2919 669
Size: 988 281 559 176 246 3079
Fragments arranged by size:
3079 988 559 281 246 176
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5329
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 5205 5205
Size: 5329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4443 4627 1305
Size: 3138 184 2007
Fragments arranged by size:
3138 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 2961
Fragments arranged by size:
2961 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3287 3646 3805 518
Size: 648 2121 359 159 2042
Fragments arranged by size:
2121 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2745 3422 4570 4815 1606
Size: 604 535 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 535 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2251 2687 4232 1132
Size: 97 1022 436 1545 2229
Fragments arranged by size:
2229 1545 1022 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5329
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PmlI CAC'GTG
Cuts at: 2311 2311
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2479
Size: 5329
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PstI C_TGCA'G
Cuts at: 830 2681 830
Size: 1851 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
PvuII CAG'CTG
Cuts at: 2210 2210
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2495 2671 1936
Size: 559 176 4594
Fragments arranged by size:
4594 559 176
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 3577 5289 3577
Size: 1712 3617
SfcI C'TryA_G
Cuts at: 826 1045 2677 3812 4493 4684 826
Size: 219 1632 1135 681 191 1471
Fragments arranged by size:
1632 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2656 1872
Size: 784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2401 3433 4334 4596 820
Size: 197 926 458 1032 901 262 1553
Fragments arranged by size:
1553 1032 926 901 458 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 2452 3253 5
Size: 47 2400 801 2081
Fragments arranged by size:
2400 2081 801 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5329
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 2768 513
Size: 552 939 471 293 3074
Fragments arranged by size:
3074 939 552 471 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2618 3356 3373 3526 3711 4156 325
Size: 1255 1038 738 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1038 738 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3575 5287 96
Size: 274 80 33 51 1443 1598 1712 138
Fragments arranged by size:
1712 1598 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5289 5321 5289
Size: 32 5297
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5329
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2654 1870
Size: 784 4545
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3137 276
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI