Restriction map for
pFN21AE0270
(Circular) (Six-base) MAPSORT of: pFN21AE0270.seq Check: 4808 from: 1 to: 5437
pFN21AE0270 5437 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2421 3500 4884 5152 1632
Size: 789 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 789 268
AfeI AGC'GCT
Cuts at: 2186 2186
Size: 5437
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 2722 4336 5313 1203
Size: 1519 1614 977 1327
Fragments arranged by size:
1614 1519 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 2723 4166 2723
Size: 1443 3994
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 2687 3180 4752 2687
Size: 493 1572 3372
Fragments arranged by size:
3372 1572 493
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 1870
Size: 73 819 4545
Fragments arranged by size:
4545 819 73
BamHI G'GATC_C
Cuts at: 2008 2767 3134 2008
Size: 759 367 4311
Fragments arranged by size:
4311 759 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2758 4213 618
Size: 325 217 627 228 743 1455 1842
Fragments arranged by size:
1842 1455 743 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2066 2756 1391
Size: 675 690 4072
Fragments arranged by size:
4072 690 675
BbeI G_GCGC'C
Cuts at: 2019 2019
Size: 5437
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BbvCI CC'TCA_GC
Cuts at: 2329 2329
Size: 5437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2675 3329 4539 2675
Size: 654 1210 3573
Fragments arranged by size:
3573 1210 654
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2113 4048 136
Size: 107 122 71 631 605 441 1935 1525
Fragments arranged by size:
1935 1525 631 605 441 122 107 71
BglII A'GATC_T
Cuts at: 2286 5432 2286
Size: 3146 2291
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 3498 4654 1790
Size: 1708 1156 2573
Fragments arranged by size:
2573 1708 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2547 3168 4126
Size: 416 53 83 399 24 1130 621 958
Cuts at: 4126 442
Size: 1753
Fragments arranged by size:
1753 1130 958 621 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4097 1239
Size: 2858 2579
Bpu10I CC'TnA_GC
Cuts at: 2329 2329
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 3244 3626 4261
Size: 53 83 186 936 483 1228 382 635
Cuts at: 4261 275
Size: 1451
Fragments arranged by size:
1451 1228 936 635 483 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 4640 5136 4640
Size: 496 4941
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4864
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 2887 3648 4676 1540
Size: 275 186 120 766 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2756 3498 3583 4654 1391
Size: 399 276 690 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 690 399 276 85
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 2291 2792 844
Size: 1281 166 501 3489
Fragments arranged by size:
3489 1281 501 166
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2116 4081 4268 1700
Size: 110 306 1965 187 2869
Fragments arranged by size:
2869 1965 306 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2179
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2008 2286 2767 3134 3519 3536 4988 5074
Size: 36 278 481 367 385 17 1452 86
Cuts at: 5074 5086 5432 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 481 385 367 346 278 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2155 2226 513
Size: 552 939 151 71 3724
Fragments arranged by size:
3724 939 552 151 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2118 2884
Size: 54 1425 50 275 18 253 35 766
Cuts at: 2884 3773 5402 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 766 275 253 54 50
43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 2884 1537
Size: 275 306 766 4090
Fragments arranged by size:
4090 766 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2421 3500 4097 4884 5152 1239
Size: 393 789 1079 597 787 268 1524
Fragments arranged by size:
1524 1079 789 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
EcoRV GAT'ATC
Cuts at: 2374 2374
Size: 5437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 5437
FseI GG_CCGG'CC
Cuts at: 2120 2120
Size: 5437
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2019 2075 2188 4584 1678
Size: 341 56 113 2396 2531
Fragments arranged by size:
2531 2396 341 113 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 2709 2741 4078
Size: 32 135 32 699 32 406 32 1337
Cuts at: 4078 4110 4152 4184 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1337 699 406 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2781 3027 669
Size: 988 281 298 545 246 3079
Fragments arranged by size:
3079 988 545 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KasI G'GCGC_C
Cuts at: 2015 2015
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 2454 4551 4735 1305
Size: 1138 11 2097 184 2007
Fragments arranged by size:
2097 2007 1138 184 11
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3615
Fragments arranged by size:
3615 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3395 3754 3913 518
Size: 648 1374 855 359 159 2042
Fragments arranged by size:
2042 1374 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2853 3530 4678 4923 1606
Size: 551 696 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 551 245
NaeI GCC'GGC
Cuts at: 2118 2118
Size: 5437
NarI GG'CG_CC
Cuts at: 2016 2016
Size: 5437
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NgoMIV G'CCGG_C
Cuts at: 2116 2116
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2726 2795 4340 1132
Size: 97 1497 69 1545 2229
Fragments arranged by size:
2229 1545 1497 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 2722 4336 2722
Size: 1614 3823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2028 2789 830
Size: 1198 761 3478
Fragments arranged by size:
3478 1198 761
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SacII CC_GC'GG
Cuts at: 2158 2158
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2024 2253 2785 3920 4601 4792 826
Size: 219 979 229 532 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 681 532 229 219 191
SfoI GGC'GCC
Cuts at: 2017 2017
Size: 5437
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2213 1517
Size: 696 4741
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 2565 2876 513
Size: 552 939 345 216 311 3074
Fragments arranged by size:
3074 939 552 345 311 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 3464 3481 3634 3819 4264 325
Size: 1255 639 1245 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1245 639 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI