Restriction map for
pFN21AE0267
(Circular) (Six-base) MAPSORT of: pFN21AE0267.seq Check: 1176 from: 1 to: 5650
pFN21AE0267 5650 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2878 2878
Size: 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2444 3460 278
Size: 53 83 186 936 908 1016 2468
Fragments arranged by size:
2468 1016 936 908 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaI G_GGCC'C
Cuts at: 2038 2038
Size: 5650
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2959 3185 3337 5446 1493
Size: 1466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2285 2975 1870
Size: 73 342 690 4545
Fragments arranged by size:
4545 690 342 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2281 2357 2713 2856
Size: 325 217 627 447 47 76 356 143
Cuts at: 2856 2890 2904 2971 4426 618
Size: 34 14 67 1455 1842
Fragments arranged by size:
1842 1455 627 447 356 325 217 143
76 67 47 34 14
BanII G_rGCy'C
Cuts at: 1391 2038 2165 2380 2480 2678 2969 1391
Size: 647 127 215 100 198 291 4072
Fragments arranged by size:
4072 647 291 215 198 127 100
BbeI G_GCGC'C
Cuts at: 2238 2285 2361 2908 2238
Size: 47 76 547 4980
Fragments arranged by size:
4980 547 76 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 928
Size: 971 340 4339
Fragments arranged by size:
4339 971 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2663 4261 136
Size: 107 122 71 631 605 991 1598 1525
Fragments arranged by size:
1598 1525 991 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2517 2718 2859 2895 3711 4867 1790
Size: 248 479 201 141 36 816 1156 2573
Fragments arranged by size:
2573 1156 816 479 248 201 141 36
BmgBI CAC'GTC
Cuts at: 1318 2520 2596 2605 1318
Size: 1202 76 9 4363
Fragments arranged by size:
4363 1202 76 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2705 2781 4310 1239
Size: 830 636 76 1529 2579
Fragments arranged by size:
2579 1529 830 636 76
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 3441 493
Size: 711 1227 1010 2702
Fragments arranged by size:
2702 1227 1010 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 2282 2358 2441
Size: 53 83 186 936 702 47 76 83
Cuts at: 2441 2905 3457 3839 4474 275
Size: 464 552 382 635 1451
Fragments arranged by size:
1451 936 702 635 552 464 382 186
83 83 76 53 47
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2082 2112 2255 2285 1178
Size: 30 165 30 679 30 143 30 4543
Fragments arranged by size:
4543 679 165 143 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 2147 1186
Size: 823 138 4689
Fragments arranged by size:
4689 823 138
BseYI C'CCAG_C
Cuts at: 2029 2074 2230 2317 2456 4853 5349 2029
Size: 45 156 87 139 2397 496 2330
Fragments arranged by size:
2397 2330 496 156 139 87 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2618 2906 1163
Size: 573 582 300 288 3907
Fragments arranged by size:
3907 582 573 300 288
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2367 2509 2690 3100 3861 4889
Size: 275 186 366 142 181 410 761 1028
Cuts at: 4889 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 410 366 275 186 181
142
BsiHKAI G_wGCw'C
Cuts at: 2178 2678 2706 3711 3796 4867 2178
Size: 500 28 1005 85 1071 2961
Fragments arranged by size:
2961 1071 1005 500 85 28
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2165 2178 2380 2480 2517 2678
Size: 399 248 127 13 202 100 37 161
Cuts at: 2678 2706 2718 2859 2895 2969 3711 3796 4867
Size: 28 12 141 36 74 742 85 1071
Cuts at: 4867 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 248 202 161 141
127 100 85 74 37 36 28 13
12
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2401 2878 3005 844
Size: 1557 477 127 3489
Fragments arranged by size:
3489 1557 477 127
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2742 4145 4319 65
Size: 1183 1494 1403 174 1396
Fragments arranged by size:
1494 1403 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2366 4294 4481 1700
Size: 110 556 1928 187 2869
Fragments arranged by size:
2869 1928 556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2336 3710 4722 2336
Size: 1374 1012 3264
Fragments arranged by size:
3264 1374 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2277 2277
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 2690 513
Size: 552 1625 3473
Fragments arranged by size:
3473 1625 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 3898
Fragments arranged by size:
3898 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 3166 3960 3980 2178
Size: 988 794 20 3848
Fragments arranged by size:
3848 988 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DraIII CAC_nnn'GTG
Cuts at: 2173 2625 2173
Size: 452 5198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2506 2544
Size: 54 1425 50 275 18 534 142 38
Cuts at: 2544 2613 2687 2798 2941 3097 3986 5615 8
Size: 69 74 111 143 156 889 1629 43
Fragments arranged by size:
1629 1425 889 534 275 156 143 142
111 74 69 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2364 2506 2687 3097 1537
Size: 275 552 142 181 410 4090
Fragments arranged by size:
4090 552 410 275 181 142
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2276 2644 4237 4611 4757 387
Size: 1064 138 687 368 1593 374 146 1280
Fragments arranged by size:
1593 1280 1064 687 374 368 146 138
EcoICRI GAG'CTC
Cuts at: 2676 2676
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2705 2781 3713 4310 5097 5365
Size: 393 437 636 76 932 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 932 787 636 597 437 393 268
76
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2204
Size: 5650
EcoO109I rG'GnC_Cy
Cuts at: 2034 2035 2034
Size: 1 5649
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2238 2285 2361 2453 2908 4797 1678
Size: 560 47 76 92 455 1889 2531
Fragments arranged by size:
2531 1889 560 455 92 76 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2636 2668 4291
Size: 32 135 32 683 32 349 32 1623
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1623 683 349 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KasI G'GCGC_C
Cuts at: 2234 2281 2357 2904 2234
Size: 47 76 547 4980
Fragments arranged by size:
4980 547 76 47
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2774 4764 4948 1305
Size: 1469 1990 184 2007
Fragments arranged by size:
2007 1990 1469 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 2800 2943 10
Size: 54 1425 343 783 185 143 2717
Fragments arranged by size:
2717 1425 783 343 185 143 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2621 3608 3967 4126 518
Size: 648 843 612 987 359 159 2042
Fragments arranged by size:
2042 987 843 648 612 359 159
MspA1I CmG'CkG
Cuts at: 1606 2692 3066 3743 4891 5136 1606
Size: 1086 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 677 374 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 5650
NarI GG'CG_CC
Cuts at: 2235 2282 2358 2905 2235
Size: 47 76 547 4980
Fragments arranged by size:
4980 547 76 47
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PspOMI G'GGCC_C
Cuts at: 2034 2034
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2205 3002 830
Size: 1375 797 3478
Fragments arranged by size:
3478 1375 797
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SacI G_AGCT'C
Cuts at: 2678 2678
Size: 5650
SacII CC_GC'GG
Cuts at: 2693 2693
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SexAI A'CCwGG_T
Cuts at: 2169 2169
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2201 2998 4133 4814 5005 826
Size: 219 1156 797 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 797 681 219 191
SfoI GGC'GCC
Cuts at: 2236 2283 2359 2906 2236
Size: 47 76 547 4980
Fragments arranged by size:
4980 547 76 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2929 1517
Size: 1412 4238
StyI C'CwwG_G
Cuts at: 513 1065 2241 3089 513
Size: 552 1176 848 3074
Fragments arranged by size:
3074 1176 848 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 3694 3847 4032 4477 325
Size: 1255 2097 17 153 185 445 1498
Fragments arranged by size:
2097 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3896 5608 96
Size: 274 80 33 51 1443 1919 1712 138
Fragments arranged by size:
1919 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2082 2114 5610 5642 2082
Size: 32 3496 32 2090
Fragments arranged by size:
3496 2090 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 2629 2874 2554
Size: 75 245 5330
Fragments arranged by size:
5330 245 75
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2442 3458 276
Size: 53 83 186 936 908 1016 2468
Fragments arranged by size:
2468 1016 936 908 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SanDI SapI SfiI SrfI SwaI