Restriction map for
pFN21AE0266
(Circular) (Six-base) MAPSORT of: pFN21AE0266.seq Check: 7514 from: 1 to: 6055
pFN21AE0266 6055 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 2790
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2682 3865 278
Size: 53 83 186 936 1146 1183 2468
Fragments arranged by size:
2468 1183 1146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3141 4118 5502 5770 1632
Size: 1509 977 1384 268 1917
Fragments arranged by size:
1917 1509 1384 977 268
AfeI AGC'GCT
Cuts at: 3245 3245
Size: 6055
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 2721 4954 5931 1203
Size: 1518 2233 977 1327
Fragments arranged by size:
2233 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2402 1166
Size: 1236 4819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2750 1184
Size: 1566 4489
AlwNI CAG_nnn'CTG
Cuts at: 2167 2230 2854 3352 3798 5370 2167
Size: 63 624 498 446 1572 2852
Fragments arranged by size:
2852 1572 624 498 446 63
ApaI G_GGCC'C
Cuts at: 3019 3149 3019
Size: 130 5925
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 3364 3590 3742 5851 1493
Size: 1871 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2541 3376 4831 618
Size: 325 217 627 636 118 835 1455 1842
Fragments arranged by size:
1842 1455 835 636 627 325 217 118
BanII G_rGCy'C
Cuts at: 1391 2013 2670 3019 3072 3149 3374 1391
Size: 622 657 349 53 77 225 4072
Fragments arranged by size:
4072 657 622 349 225 77 53
BbeI G_GCGC'C
Cuts at: 2427 2427
Size: 6055
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2836 928
Size: 971 937 4147
Fragments arranged by size:
4147 971 937
BbvCI CC'TCA_GC
Cuts at: 2027 2079 2027
Size: 52 6003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3153 3187 4246 4280 1903
Size: 34 1216 34 1059 34 3678
Fragments arranged by size:
3678 1216 1059 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3947 5157 2241
Size: 1706 1210 3139
Fragments arranged by size:
3139 1706 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2421 2582 2632
Size: 107 122 71 631 605 749 161 50
Cuts at: 2632 3076 4666 136
Size: 444 1590 1525
Fragments arranged by size:
1590 1525 749 631 605 444 161 122
107 71 50
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2544 3019 3149 4116 5272 1790
Size: 347 407 475 130 967 1156 2573
Fragments arranged by size:
2573 1156 967 475 407 347 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2979 2990 3106
Size: 416 53 83 399 24 1562 11 116
Cuts at: 3106 3786 4744 442
Size: 680 958 1753
Fragments arranged by size:
1753 1562 958 680 416 399 116 83
53 24 11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2367 4715 1239
Size: 962 166 2348 2579
Fragments arranged by size:
2579 2348 962 166
Bpu10I CC'TnA_GC
Cuts at: 2027 2079 2027
Size: 52 6003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 4180 5045 5343 2081
Size: 2099 865 298 2793
Fragments arranged by size:
2793 2099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2134 4718 882
Size: 617 635 2584 2219
Fragments arranged by size:
2584 2219 635 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3199 3846 493
Size: 711 1518 477 647 2702
Fragments arranged by size:
2702 1518 711 647 477
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2424 2496 2679 2817
Size: 53 83 186 936 891 72 183 138
Cuts at: 2817 3862 4244 4879 275
Size: 1045 382 635 1451
Fragments arranged by size:
1451 1045 936 891 635 382 186 183
138 83 72 53
BsaWI w'CCGG_w
Cuts at: 2013 4488 4886 5160 5307 5718 2013
Size: 2475 398 274 147 411 2350
Fragments arranged by size:
2475 2350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6055
BseYI C'CCAG_C
Cuts at: 2324 2433 2499 2617 2812 3025 3139 3240 5258
Size: 109 66 118 195 213 114 101 2018
Cuts at: 5258 5754 2324
Size: 496 2625
Fragments arranged by size:
2625 2018 496 213 195 118 114 109
101 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3176 3365 1163
Size: 573 1440 189 3853
Fragments arranged by size:
3853 1440 573 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2561 2880 3505 4266 5294 1540
Size: 275 186 560 319 625 761 1028 2301
Fragments arranged by size:
2301 1028 761 625 560 319 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 2751 4116 4201 5272 2646
Size: 105 1365 85 1071 3429
Fragments arranged by size:
3429 1365 1071 105 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2137 2544 2646 2670 2751 3019
Size: 399 223 124 407 102 24 81 268
Cuts at: 3019 3072 3149 3374 4116 4201 5272 1391
Size: 53 77 225 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 268 225 223
124 102 85 81 77 53 24
BspEI T'CCGG_A
Cuts at: 2013 5718 2013
Size: 3705 2350
BspHI T'CATG_A
Cuts at: 2961 3324 3944 5674 2961
Size: 363 620 1730 3342
Fragments arranged by size:
3342 1730 620 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2739 2790 2976 3410 844
Size: 1895 51 186 434 3489
Fragments arranged by size:
3489 1895 434 186 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2770 2893 3942 1394
Size: 429 947 123 1049 3507
Fragments arranged by size:
3507 1049 947 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 3207 4550 4724 65
Size: 1183 1959 1343 174 1396
Fragments arranged by size:
1959 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2443 2626 2875 4699 4886 1700
Size: 110 275 358 183 249 1824 187 2869
Fragments arranged by size:
2869 1824 358 275 249 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 2461 3155 4115 5127 2461
Size: 694 960 1012 3389
Fragments arranged by size:
3389 1012 960 694
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2463 794
Size: 1669 4386
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 3319 3319
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050
Size: 1413 367 385 17 1452 86 12 346
Cuts at: 6050 1972
Size: 1977
Fragments arranged by size:
1977 1452 1413 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2925 3312 513
Size: 552 939 921 387 3256
Fragments arranged by size:
3256 939 921 552 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3571 4365 4385 2503
Size: 1068 794 20 4173
Fragments arranged by size:
4173 1068 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2342 2445 2558
Size: 54 1425 50 275 18 512 103 113
Cuts at: 2558 2859 2877 2913 3297 3502 4391 6020 8
Size: 301 18 36 384 205 889 1629 43
Fragments arranged by size:
1629 1425 889 512 384 301 275 205
113 103 54 50 43 36 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2558 2877 3502 1537
Size: 275 746 319 625 4090
Fragments arranged by size:
4090 746 625 319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 2600 2981 4642 5016 5162
Size: 1064 138 791 220 381 1661 374 146
Cuts at: 5162 387
Size: 1280
Fragments arranged by size:
1661 1280 1064 791 381 374 220 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2367 3141 4118 4715 5502 5770
Size: 393 569 166 774 977 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1524 977 787 774 597 569 393 268
166
EcoNI CCTnn'n_nnAGG
Cuts at: 3010 3123 3010
Size: 113 5942
EcoO109I rG'GnC_Cy
Cuts at: 2284 3015 3016 3043 3146 3349 2284
Size: 731 1 27 103 203 4990
Fragments arranged by size:
4990 731 203 103 27 1
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2427 2571 2837 3247 3268 5202 1678
Size: 749 144 266 410 21 1934 2531
Fragments arranged by size:
2531 1934 749 410 266 144 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 2792 2824 4696
Size: 32 135 32 652 32 536 32 1872
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1872 652 536 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2423 2423
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2915 3299 10
Size: 54 1425 343 1083 384 2766
Fragments arranged by size:
2766 1425 1083 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2402 2976 4013 4372 4531 518
Size: 648 1041 195 574 1037 359 159 2042
Fragments arranged by size:
2042 1041 1037 648 574 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 3143 3471 4148 5296 5541 1606
Size: 1537 328 677 1148 245 2120
Fragments arranged by size:
2120 1537 1148 677 328 245
NaeI GCC'GGC
Cuts at: 2087 2445 2628 2087
Size: 358 183 5514
Fragments arranged by size:
5514 358 183
NarI GG'CG_CC
Cuts at: 2424 2424
Size: 6055
NcoI C'CATG_G
Cuts at: 513 1065 2004 2925 3312 513
Size: 552 939 921 387 3256
Fragments arranged by size:
3256 939 921 552 387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2085 2443 2626 2085
Size: 358 183 5514
Fragments arranged by size:
5514 358 183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 2558 3502 2558
Size: 944 5111
NspI r_CATG'y
Cuts at: 1132 1229 2626 2872 3413 4958 1132
Size: 97 1397 246 541 1545 2229
Fragments arranged by size:
2229 1545 1397 541 246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PfoI T'CCnGG_A
Cuts at: 2525 2525
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2284
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 2927 1613
Size: 1314 4741
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 3015 3145 3015
Size: 130 5925
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 6023
PstI C_TGCA'G
Cuts at: 830 2065 2575 3407 830
Size: 1235 510 832 3478
Fragments arranged by size:
3478 1235 832 510
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 2284 2284
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 2906 4303 6015 2906
Size: 1397 1712 2946
Fragments arranged by size:
2946 1712 1397
SexAI A'CCwGG_T
Cuts at: 2223 2223
Size: 6055
SfcI C'TryA_G
Cuts at: 826 1045 2061 2571 3403 4538 5219 5410 826
Size: 219 1016 510 832 1135 681 191 1471
Fragments arranged by size:
1471 1135 1016 832 681 510 219 191
SfoI GGC'GCC
Cuts at: 2425 2425
Size: 6055
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 4159 5060 5322 820
Size: 197 926 153 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 2626 2872 3413 1132
Size: 1494 246 541 3774
Fragments arranged by size:
3774 1494 541 246
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 2925 3312 3494 513
Size: 552 939 921 387 182 3074
Fragments arranged by size:
3074 939 921 552 387 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 4301 6013
Size: 274 80 33 51 1443 927 1397 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1443 1397 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2937 2969 6015 6047 2937
Size: 32 3046 32 2945
Fragments arranged by size:
3046 2945 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2191 1316
Size: 875 5180
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2923 3003 2401
Size: 522 80 5453
Fragments arranged by size:
5453 522 80
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2680 3863 276
Size: 53 83 186 936 1146 1183 2468
Fragments arranged by size:
2468 1183 1146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PvuII RsrII SacI
SacII SapI SfiI SrfI SwaI