Restriction map for
pFN21AE0264
(Circular) (Six-base) MAPSORT of: pFN21AE0264.seq Check: 1276 from: 1 to: 5950
pFN21AE0264 5950 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2719 2719
Size: 5950
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2908 3293 1937
Size: 971 385 4594
Fragments arranged by size:
4594 971 385
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2764 820
Size: 197 1747 4006
Fragments arranged by size:
4006 1747 197
AflIII A'CryG_T
Cuts at: 1203 2099 4849 5826 1203
Size: 896 2750 977 1327
Fragments arranged by size:
2750 1327 977 896
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 2546 1166
Size: 1380 4570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3191 1184
Size: 2007 3943
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3040 3259 3485 3637 5746 1493
Size: 1547 219 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 219 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 3275 1870
Size: 73 300 1032 4545
Fragments arranged by size:
4545 1032 300 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 3271 4726 618
Size: 325 217 627 239 1245 1455 1842
Fragments arranged by size:
1842 1455 1245 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2347 3269 1391
Size: 956 922 4072
Fragments arranged by size:
4072 956 922
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 5950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 3107 4011 5167 1790
Size: 1317 904 1156 2573
Fragments arranged by size:
2573 1317 1156 904
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2175 2361 2945 4610 1239
Size: 900 36 186 584 1665 2579
Fragments arranged by size:
2579 1665 900 584 186 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 4613 882
Size: 617 581 2533 2219
Fragments arranged by size:
2533 2219 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 3757 4139 4774
Size: 53 83 186 936 494 1730 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1730 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 4764
Fragments arranged by size:
4764 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3101 1186
Size: 1915 4035
BseYI C'CCAG_C
Cuts at: 2058 5153 5649 2058
Size: 3095 496 2359
Fragments arranged by size:
3095 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 2697 1163
Size: 573 660 301 4416
Fragments arranged by size:
4416 660 573 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 4096 5167 4011
Size: 85 1071 4794
Fragments arranged by size:
4794 1071 85
BsmI GAATG_Cn'
Cuts at: 2109 3461 3554 2109
Size: 1352 93 4505
Fragments arranged by size:
4505 1352 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 3107 3269 4011 4096 5167 1391
Size: 399 557 760 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 557 399 162 85
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3192 3839 5569 3192
Size: 647 1730 3573
Fragments arranged by size:
3573 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2719 3305 844
Size: 1875 586 3489
Fragments arranged by size:
3489 1875 586
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 3251 4445 4619 65
Size: 1183 1165 838 1194 174 1396
Fragments arranged by size:
1396 1194 1183 1165 838 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2960 4594 4781 1700
Size: 110 1150 1634 187 2869
Fragments arranged by size:
2869 1634 1150 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 96
Size: 2187 3763
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3102 794
Size: 2308 3642
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2300 2312 2300
Size: 12 5938
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2865 2883 513
Size: 552 1800 18 3580
Fragments arranged by size:
3580 1800 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3003 487
Size: 683 56 1777 3434
Fragments arranged by size:
3434 1777 683 56
BtsI GCAGTG_nn'
Cuts at: 2146 3107 3466 4260 4280 2146
Size: 961 359 794 20 3816
Fragments arranged by size:
3816 961 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 2500 2874 3254 2500
Size: 374 380 5196
Fragments arranged by size:
5196 380 374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 2302 2314
Size: 54 1425 50 275 18 460 12 12
Cuts at: 2314 2418 2513 3397 4286 5915 8
Size: 104 95 884 889 1629 43
Fragments arranged by size:
1629 1425 889 884 460 275 104 95
54 50 43 18 12 12
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3112 3880 1353
Size: 1759 768 3423
Fragments arranged by size:
3423 1759 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2175 2361 2945 4013 4610 5397
Size: 393 507 36 186 584 1068 597 787
Cuts at: 5397 5665 1239
Size: 268 1524
Fragments arranged by size:
1524 1068 787 597 584 507 393 268
186 36
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2030 5097 1678
Size: 352 3067 2531
Fragments arranged by size:
3067 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2905 2937 3156 3188 3196
Size: 32 135 32 1333 32 219 32 8
Cuts at: 3196 3228 4591 4623 4665 4697 1373
Size: 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 1333 219 135 42 32 32
32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2668 2897 3294 3540 669
Size: 988 281 730 229 397 246 3079
Fragments arranged by size:
3079 988 730 397 281 246 229
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 2718 3549 2718
Size: 831 5119
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2292 2304 2420 2515 10
Size: 54 1425 343 460 12 116 95 3445
Fragments arranged by size:
3445 1425 460 343 116 95 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2420 2546 3137 3908 4267 4426
Size: 648 1132 122 126 591 771 359 159
Cuts at: 4426 518
Size: 2042
Fragments arranged by size:
2042 1132 771 648 591 359 159 126
122
MspA1I CmG'CkG
Cuts at: 1606 2402 2432 3366 4043 5191 5436 1606
Size: 796 30 934 677 1148 245 2120
Fragments arranged by size:
2120 1148 934 796 677 245 30
NaeI GCC'GGC
Cuts at: 2962 2962
Size: 5950
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5398
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 2960 2960
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NruI TCG'CGA
Cuts at: 2988 2988
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2103 3308 4853 1132
Size: 97 874 1205 1545 2229
Fragments arranged by size:
2229 1545 1205 874 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 2099 4849 2099
Size: 2750 3200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2152 3222 2152
Size: 1070 4880
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3089 1613
Size: 1476 4474
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2536 2779 3302 830
Size: 1706 243 523 3478
Fragments arranged by size:
3478 1706 523 243
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2402 2402
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 2536 3302 2536
Size: 766 5184
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2532 2775 2829 3298 4433 5114 5305
Size: 219 1487 243 54 469 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1487 1471 1135 681 469 243 219 191
54
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 5950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2764 4054 4955 5217 820
Size: 197 926 300 521 1290 901 262 1553
Fragments arranged by size:
1553 1290 926 901 521 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 3389 513
Size: 552 2324 3074
Fragments arranged by size:
3074 2324 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 3977 3994 4147 4332 4777 325
Size: 1255 492 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 492 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2180 2283 4196
Size: 274 80 33 51 1443 203 103 1913
Cuts at: 4196 5908 96
Size: 1712 138
Fragments arranged by size:
1913 1712 1443 274 203 138 103 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2334 2366 5910 5942 2334
Size: 32 3544 32 2342
Fragments arranged by size:
3544 2342 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 2243 1943
Size: 300 5650
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2148 4079 2148
Size: 1931 4019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI