Restriction map for
pFN21AE0261
(Circular) (Six-base) MAPSORT of: pFN21AE0261.seq Check: 684 from: 1 to: 5830
pFN21AE0261 5830 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2352 3893 5277 5545 1632
Size: 542 178 1541 1384 268 1917
Fragments arranged by size:
1917 1541 1384 542 268 178
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 2678 3573 5145 2678
Size: 895 1572 3363
Fragments arranged by size:
3363 1572 895
ApaI G_GGCC'C
Cuts at: 2596 2596
Size: 5830
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2907 3151 4606 618
Size: 325 217 627 1120 244 1455 1842
Fragments arranged by size:
1842 1455 1120 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2223 2596 3149 1391
Size: 832 373 553 4072
Fragments arranged by size:
4072 832 553 373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BbvCI CC'TCA_GC
Cuts at: 2064 2064
Size: 5830
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 4021 4055 1903
Size: 34 152 34 1898 34 3678
Fragments arranged by size:
3678 1898 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2564 4441 136
Size: 107 122 71 631 605 892 1877 1525
Fragments arranged by size:
1877 1525 892 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2718 3247 2718
Size: 529 5301
Bme1580I G_kGCm'C
Cuts at: 1790 2596 3891 5047 1790
Size: 806 1295 1156 2573
Fragments arranged by size:
2573 1295 1156 806
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2268 3561 4519
Size: 416 53 83 399 24 851 1293 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1293 958 851 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4490 1239
Size: 3251 2579
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2079 3955 4820 5118 2079
Size: 1876 865 298 2791
Fragments arranged by size:
2791 1876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 2779 4493 882
Size: 617 792 488 1714 2219
Fragments arranged by size:
2219 1714 792 617 488
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2672 2933 2999 3062 3065 1186
Size: 895 591 261 66 63 3 3951
Fragments arranged by size:
3951 895 591 261 66 63 3
BseYI C'CCAG_C
Cuts at: 2024 5033 5529 2024
Size: 3009 496 2325
Fragments arranged by size:
3009 2325 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 3280 4041 5069 1540
Size: 275 186 112 1167 761 1028 2301
Fragments arranged by size:
2301 1167 1028 761 275 186 112
BsiHKAI G_wGCw'C
Cuts at: 2223 3891 3976 5047 2223
Size: 1668 85 1071 3006
Fragments arranged by size:
3006 1668 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
BsmBI CGTCTCn'nnnn_
Cuts at: 2576 2576
Size: 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2596 3149 3891 3976 5047 1391
Size: 399 433 373 553 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 433 399 373 85
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3118 3185 844
Size: 2274 67 3489
Fragments arranged by size:
3489 2274 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 3717 1394
Size: 429 196 1698 3507
Fragments arranged by size:
3507 1698 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 2035 4325 4499 65
Size: 1183 787 2290 174 1396
Fragments arranged by size:
2290 1396 1183 787 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3124 794
Size: 2330 3500
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2588 2588
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5830
BtgI C'CryG_G
Cuts at: 513 1065 2012 513
Size: 552 947 4331
Fragments arranged by size:
4331 947 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2977 3346 4140 4160 2413
Size: 564 369 794 20 4083
Fragments arranged by size:
4083 794 564 369 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2029 2064 529
Size: 35 1465 35 4295
Fragments arranged by size:
4295 1465 35 35
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2110 2238 3277
Size: 54 1425 50 275 18 280 128 1039
Cuts at: 3277 4166 5795 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 280 275 128 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2110 3277 1537
Size: 275 298 1167 4090
Fragments arranged by size:
4090 1167 298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2894 2910 3026 3760 1353
Size: 875 666 16 116 734 3423
Fragments arranged by size:
3423 875 734 666 116 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 2221
Size: 5830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2352 3893 4490 5277 5545 1239
Size: 393 542 178 1541 597 787 268 1524
Fragments arranged by size:
1541 1524 787 597 542 393 268 178
EcoNI CCTnn'n_nnAGG
Cuts at: 2291 2291
Size: 5830
EcoO109I rG'GnC_Cy
Cuts at: 2584 2592 2593 2584
Size: 8 1 5821
Fragments arranged by size:
5821 8 1
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2528 748
Size: 1780 4050
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3788 4147 4306 518
Size: 648 2622 359 159 2042
Fragments arranged by size:
2622 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2074 3246 3923 5071 5316 1606
Size: 408 60 1172 677 1148 245 2120
Fragments arranged by size:
2120 1172 1148 677 408 245 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 3004 387
Size: 2617 3213
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2717 3127 3188 4733 1132
Size: 97 1488 410 61 1545 2229
Fragments arranged by size:
2229 1545 1488 410 97 61
PasI CC'CwG_GG
Cuts at: 1348 2588 1348
Size: 1240 4590
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2584 2584
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 2310 3400 2310
Size: 1090 4740
PspOMI G'GGCC_C
Cuts at: 2592 2592
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2408 2607 2827 2968 3182 830
Size: 1578 199 220 141 214 3478
Fragments arranged by size:
3478 1578 220 214 199 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5830
SacI G_AGCT'C
Cuts at: 2223 2223
Size: 5830
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2228 2228
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2404 2603 2670 2823 2964 3178 4313
Size: 219 1359 199 67 153 141 214 1135
Cuts at: 4313 4994 5185 826
Size: 681 191 1471
Fragments arranged by size:
1471 1359 1135 681 219 214 199 191
153 141 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2094 3934 4835 5097 820
Size: 197 926 151 1840 901 262 1553
Fragments arranged by size:
1840 1553 926 901 262 197 151
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3127 3188 1132
Size: 1995 61 3774
Fragments arranged by size:
3774 1995 61
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2106 2558 1517
Size: 589 452 4789
Fragments arranged by size:
4789 589 452
StyI C'CwwG_G
Cuts at: 513 1065 2190 3269 513
Size: 552 1125 1079 3074
Fragments arranged by size:
3074 1125 1079 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2721 3857 3874 4027 4212 4657 325
Size: 1255 1141 1136 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1141 1136 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2272 4076 5788
Size: 274 80 33 51 1443 295 1804 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1804 1712 1443 295 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 2036 1316
Size: 720 5110
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2248
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI