Restriction map for
pFN21AE0259
(Circular) (Six-base) MAPSORT of: pFN21AE0259.seq Check: 8870 from: 1 to: 5455
pFN21AE0259 5455 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2005 2167 2005
Size: 162 5293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 278
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2798 1937
Size: 861 4594
Acc65I G'GTAC_C
Cuts at: 2776 2776
Size: 5455
AclI AA'CG_TT
Cuts at: 1563 3582 3955 1563
Size: 2019 373 3063
Fragments arranged by size:
3063 2019 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2453 2500 3518 4902 5170 1632
Size: 398 423 47 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 423 398 268 47
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5258
AflIII A'CryG_T
Cuts at: 1203 4354 5331 1203
Size: 3151 977 1327
Fragments arranged by size:
3151 1327 977
AgeI A'CCGG_T
Cuts at: 4286 4286
Size: 5455
AhdI GACnn_n'nnGTC
Cuts at: 4184 4184
Size: 5455
AleI CACnn'nnGTG
Cuts at: 1166 2153 2607 1166
Size: 987 454 4014
Fragments arranged by size:
4014 987 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 1184
Size: 1032 4423
AlwNI CAG_nnn'CTG
Cuts at: 2548 3198 4770 2548
Size: 650 1572 3233
Fragments arranged by size:
3233 1572 650
ApaLI G'TGCA_C
Cuts at: 2273 3512 4668 2273
Size: 1239 1156 3060
Fragments arranged by size:
3060 1239 1156
ApoI r'AATT_y
Cuts at: 1493 2764 2990 3142 5251 1493
Size: 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4009 160
Size: 872 2977 1606
Fragments arranged by size:
2977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
AvaI C'yCGr_G
Cuts at: 1870 1943 2658 2780 1870
Size: 73 715 122 4545
Fragments arranged by size:
4545 715 122 73
BamHI G'GATC_C
Cuts at: 2785 3152 2785
Size: 367 5088
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 4231 618
Size: 325 217 627 989 1455 1842
Fragments arranged by size:
1842 1455 989 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2262 2322 2351 2412 2463 2774 1391
Size: 871 60 29 61 51 311 4072
Fragments arranged by size:
4072 871 311 61 60 51 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2062 2506 928
Size: 971 163 444 3877
Fragments arranged by size:
3877 971 444 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3646 3680 1903
Size: 34 1709 34 3678
Fragments arranged by size:
3678 1709 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 4557 3347
Size: 1210 4245
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4066 136
Size: 107 122 71 631 605 2394 1525
Fragments arranged by size:
2394 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5450 5450
Size: 5455
BlpI GC'TnA_GC
Cuts at: 2291 2872 2291
Size: 581 4874
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3516 4672 1790
Size: 487 1239 1156 2573
Fragments arranged by size:
2573 1239 1156 487
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4144 442
Size: 416 53 83 399 24 1769 958 1753
Fragments arranged by size:
1769 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2054 2084 2606 2660 2734 4115 1239
Size: 809 6 30 522 54 74 1381 2579
Fragments arranged by size:
2579 1381 809 522 74 54 30 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3580 4445 4743 3580
Size: 865 298 4292
Fragments arranged by size:
4292 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4118 882
Size: 617 2619 2219
Fragments arranged by size:
2619 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3246 493
Size: 711 2042 2702
Fragments arranged by size:
2702 2042 711
BsaBI GATnn'nnATC
Cuts at: 5449 5449
Size: 5455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3262 3644 4279 275
Size: 53 83 186 936 1729 382 635 1451
Fragments arranged by size:
1729 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2280 2484 3888 4286 4560 4707 5118 2280
Size: 204 1404 398 274 147 411 2617
Fragments arranged by size:
2617 1404 411 398 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5230
Fragments arranged by size:
5230 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2582 1186
Size: 1234 162 4059
Fragments arranged by size:
4059 1234 162
BseYI C'CCAG_C
Cuts at: 2344 2520 4658 5154 2344
Size: 176 2138 496 2645
Fragments arranged by size:
2645 2138 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4882
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2905 3666 4694 1540
Size: 275 186 904 761 1028 2301
Fragments arranged by size:
2301 1028 904 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2322 2412 2463 3516 3601 4672 2277
Size: 45 90 51 1053 85 1071 3060
Fragments arranged by size:
3060 1071 1053 90 85 51 45
BsmI GAATG_Cn'
Cuts at: 2966 3059 2966
Size: 93 5362
BsmBI CGTCTCn'nnnn_
Cuts at: 2215 2383 2215
Size: 168 5287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2277 2322 2351 2412 2463 2774
Size: 399 472 15 45 29 61 51 311
Cuts at: 2774 3516 3601 4672 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 311 85 61
51 45 29 15
BspEI T'CCGG_A
Cuts at: 5118 5118
Size: 5455
BspHI T'CATG_A
Cuts at: 3344 5074 3344
Size: 1730 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 2167 2810 844
Size: 1161 162 643 3489
Fragments arranged by size:
3489 1161 643 162
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 2665 3342 1394
Size: 429 467 375 677 3507
Fragments arranged by size:
3507 677 467 429 375
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 3950 4124 65
Size: 1183 1462 1240 174 1396
Fragments arranged by size:
1462 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 4099 4286 1700
Size: 110 230 2059 187 2869
Fragments arranged by size:
2869 2059 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5455
BssSI C'ACGA_G
Cuts at: 3515 4527 3515
Size: 1012 4443
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 794
Size: 1324 4131
BstBI TT'CG_AA
Cuts at: 4259 4259
Size: 5455
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5455
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5455
BstYI r'GATC_y
Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450
Size: 813 367 385 17 1452 86 12 346
Cuts at: 5450 1972
Size: 1977
Fragments arranged by size:
1977 1452 813 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2237 2237
Size: 5455
BtgI C'CryG_G
Cuts at: 513 1065 2004 2608 2742 513
Size: 552 939 604 134 3226
Fragments arranged by size:
3226 939 604 552 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2449 487
Size: 683 56 1223 3493
Fragments arranged by size:
3493 1223 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3765 3785 2971
Size: 794 20 4641
Fragments arranged by size:
4641 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5420
DraI TTT'AAA
Cuts at: 2759 2759
Size: 5455
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 5455
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4462 809
Size: 507 3146 1802
Fragments arranged by size:
3146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2902 3791
Size: 54 1425 50 275 18 268 804 889
Cuts at: 3791 5420 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 804 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2902 1537
Size: 275 1090 4090
Fragments arranged by size:
4090 1090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2426 3385 1353
Size: 1073 959 3423
Fragments arranged by size:
3423 1073 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 4042 4416 4562 387
Size: 1064 138 930 1523 374 146 1280
Fragments arranged by size:
1523 1280 1064 930 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2410 2461 2320
Size: 90 51 5314
Fragments arranged by size:
5314 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2048 2054 2084 2453 2500 2606
Size: 393 398 18 6 30 369 47 106
Cuts at: 2606 2660 2734 3518 4115 4902 5170 1239
Size: 54 74 784 597 787 268 1524
Fragments arranged by size:
1524 787 784 597 398 393 369 268
106 74 54 47 30 18 6
EcoO109I rG'GnC_Cy
Cuts at: 2251 2251
Size: 5455
EcoRI G'AATT_C
Cuts at: 2764 3142 2764
Size: 378 5077
FspI TGC'GCA
Cuts at: 3961 3961
Size: 5455
HaeII r_GCGC'y
Cuts at: 1678 4602 1678
Size: 2924 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 4096 4128 4170
Size: 32 135 32 1012 32 1480 32 42
Cuts at: 4170 4202 1373
Size: 32 2626
Fragments arranged by size:
2626 1480 1012 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2799 3045 669
Size: 988 281 861 246 3079
Fragments arranged by size:
3079 988 861 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5455
HpaI GTT'AAC
Cuts at: 3045 3045
Size: 5455
KpnI G_GTAC'C
Cuts at: 2780 2780
Size: 5455
MfeI C'AATT_G
Cuts at: 3054 3054
Size: 5455
MluI A'CGCG_T
Cuts at: 5331 5331
Size: 5455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 4569 4753 1305
Size: 1182 2082 184 2007
Fragments arranged by size:
2082 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3633
Fragments arranged by size:
3633 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 2607 3413 3772 3931 518
Size: 648 987 454 806 359 159 2042
Fragments arranged by size:
2042 987 806 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 2580 2638 2871 3548 4696 4941 1606
Size: 914 60 58 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 245 233 60 58
NcoI C'CATG_G
Cuts at: 513 1065 2004 2742 513
Size: 552 939 738 3226
Fragments arranged by size:
3226 939 738 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5455
NotI GC'GGCC_GC
Cuts at: 2902 2902
Size: 5455
NspI r_CATG'y
Cuts at: 1132 1229 2635 2813 4358 1132
Size: 97 1406 178 1545 2229
Fragments arranged by size:
2229 1545 1406 178 97
PasI CC'CwG_GG
Cuts at: 1348 2246 2650 1348
Size: 898 404 4153
Fragments arranged by size:
4153 898 404
PciI A'CATG_T
Cuts at: 4354 4354
Size: 5455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2172 2560 2743 1108
Size: 902 162 388 183 3820
Fragments arranged by size:
3820 902 388 183 162
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 5455
PmeI GTTT'AAAC
Cuts at: 2759 2759
Size: 5455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 5037 5069 3524
Size: 32 1481 32 3910
Fragments arranged by size:
3910 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2251
Size: 5455
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5455
PsiI TTA'TAA
Cuts at: 3025 3025
Size: 5455
PstI C_TGCA'G
Cuts at: 830 2393 2807 830
Size: 1563 414 3478
Fragments arranged by size:
3478 1563 414
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5455
PvuII CAG'CTG
Cuts at: 2520 2638 2520
Size: 118 5337
SacI G_AGCT'C
Cuts at: 2322 2412 2463 2322
Size: 90 51 5314
Fragments arranged by size:
5314 90 51
SalI G'TCGA_C
Cuts at: 1936 2797 1936
Size: 861 4594
SanDI GG'GwC_CC
Cuts at: 2251 2251
Size: 5455
SbfI CC_TGCA'GG
Cuts at: 2807 2807
Size: 5455
ScaI AGT'ACT
Cuts at: 3703 5415 3703
Size: 1712 3743
SfcI C'TryA_G
Cuts at: 826 1045 2090 2389 2803 3938 4619 4810 826
Size: 219 1045 299 414 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 681 414 299 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5455
SmaI CCC'GGG
Cuts at: 1872 2660 2782 1872
Size: 788 122 4545
Fragments arranged by size:
4545 788 122
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3559 4460 4722 820
Size: 197 926 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3246 493
Size: 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5455
SphI G_CATG'C
Cuts at: 1132 2813 1132
Size: 1681 3774
SspI AAT'ATT
Cuts at: 5 52 3379 5
Size: 47 3327 2081
Fragments arranged by size:
3327 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5455
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2262 2742 2894 513
Size: 552 939 147 111 480 152 3074
Fragments arranged by size:
3074 939 552 480 152 147 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2512 3482 3499 3652 3837 4282
Size: 1255 632 300 970 17 153 185 445
Cuts at: 4282 325
Size: 1498
Fragments arranged by size:
1498 1255 970 632 445 300 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3701 5413 96
Size: 274 80 33 51 1443 1724 1712 138
Fragments arranged by size:
1724 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5415 5447 5415
Size: 32 5423
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5455
XbaI T'CTAG_A
Cuts at: 2791 2791
Size: 5455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5455
XmaI C'CCGG_G
Cuts at: 1870 2658 2780 1870
Size: 788 122 4545
Fragments arranged by size:
4545 788 122
XmnI GAAnn'nnTTC
Cuts at: 3584 3584
Size: 5455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 276
Size: 53 83 186 936 1729 2468
Fragments arranged by size:
2468 1729 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI