Restriction map for
pFN21AE0253
(Circular) (Six-base) MAPSORT of: pFN21AE0253.seq Check: 1277 from: 1 to: 5881
pFN21AE0253 5881 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 2586 3202 2586
Size: 616 5265
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2203 2510 3944 5328 5596 1632
Size: 571 307 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 571 307 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2972 1184
Size: 1788 4093
AlwNI CAG_nnn'CTG
Cuts at: 2253 3624 5196 2253
Size: 1371 1572 2938
Fragments arranged by size:
2938 1572 1371
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2567 2720 3190 3416 3568 5677 1493
Size: 1074 153 470 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 470 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 3206 1870
Size: 73 1263 4545
Fragments arranged by size:
4545 1263 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2586 3202 4657 618
Size: 325 217 627 799 616 1455 1842
Fragments arranged by size:
1842 1455 799 627 616 325 217
BanII G_rGCy'C
Cuts at: 1391 2138 3024 3200 1391
Size: 747 886 176 4072
Fragments arranged by size:
4072 886 747 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2698 928
Size: 971 799 4111
Fragments arranged by size:
4111 971 799
BbvCI CC'TCA_GC
Cuts at: 2411 2945 2411
Size: 534 5347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3058 3773 4983 3058
Size: 715 1210 3956
Fragments arranged by size:
3956 1210 715
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 4651
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 3942 5098 1790
Size: 2152 1156 2573
Fragments arranged by size:
2573 2152 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2205 3173 3612
Size: 416 53 83 399 24 788 968 439
Cuts at: 3612 4570 442
Size: 958 1753
Fragments arranged by size:
1753 968 958 788 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 3043 2197
Size: 846 5035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2558 2812 4541 1239
Size: 1319 254 1729 2579
Fragments arranged by size:
2579 1729 1319 254
Bpu10I CC'TnA_GC
Cuts at: 2411 2945 2411
Size: 534 5347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2193 2753 4544 882
Size: 617 694 560 1791 2219
Fragments arranged by size:
2219 1791 694 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 2242 3672 493
Size: 711 1038 1430 2702
Fragments arranged by size:
2702 1430 1038 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2109 4314 4712 4986 5133 5544 2109
Size: 2205 398 274 147 411 2446
Fragments arranged by size:
2446 2205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2170 2933 1186
Size: 984 763 4134
Fragments arranged by size:
4134 984 763
BseYI C'CCAG_C
Cuts at: 2256 2929 5084 5580 2256
Size: 673 2155 496 2557
Fragments arranged by size:
2557 2155 673 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 1163
Size: 573 762 4546
Fragments arranged by size:
4546 762 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3024 3942 4027 5098 3024
Size: 918 85 1071 3807
Fragments arranged by size:
3807 1071 918 85
BsmI GAATG_Cn'
Cuts at: 2162 3392 3485 2162
Size: 1230 93 4558
Fragments arranged by size:
4558 1230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 3024 3200 3942 4027 5098 1391
Size: 399 348 886 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 399 348 176 85
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 3236 844
Size: 1535 857 3489
Fragments arranged by size:
3489 1535 857
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 3768 1394
Size: 429 761 1184 3507
Fragments arranged by size:
3507 1184 761 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2374 4525 4712 1700
Size: 110 564 2151 187 2869
Fragments arranged by size:
2869 2151 564 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 2474 3941 4953 2474
Size: 1467 1012 3402
Fragments arranged by size:
3402 1467 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 794
Size: 1691 4190
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2493 3211 3578 3963 3980 5432 5518 5530
Size: 521 718 367 385 17 1452 86 12
Cuts at: 5530 5876 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 718 521 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2636 2636
Size: 5881
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 3397 4191 4211 2264
Size: 1133 794 20 3934
Fragments arranged by size:
3934 1133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 2800 4888 809
Size: 507 798 686 2088 1802
Fragments arranged by size:
2088 1802 798 686 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2293 3328 4217
Size: 54 1425 50 275 18 463 1035 889
Cuts at: 4217 5846 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1035 889 463 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 3811 1353
Size: 1145 1313 3423
Fragments arranged by size:
3423 1313 1145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2459 2656 4468 4842 4988 387
Size: 1064 138 870 197 1812 374 146 1280
Fragments arranged by size:
1812 1280 1064 870 374 197 146 138
EcoICRI GAG'CTC
Cuts at: 3022 3022
Size: 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2510 2558 2812 3944 4541 5328
Size: 393 571 307 48 254 1132 597 787
Cuts at: 5328 5596 1239
Size: 268 1524
Fragments arranged by size:
1524 1132 787 597 571 393 307 268
254 48
EcoO109I rG'GnC_Cy
Cuts at: 2069 2329 2069
Size: 260 5621
EcoRI G'AATT_C
Cuts at: 2567 2720 3190 3568 2567
Size: 153 470 378 4880
Fragments arranged by size:
4880 470 378 153
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2955 2987 2997
Size: 32 135 32 489 32 862 32 10
Cuts at: 2997 3029 4522 4554 4596 4628 1373
Size: 32 1493 32 42 32 2626
Fragments arranged by size:
2626 1493 862 489 135 42 32 32
32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 2590 3206 2590
Size: 616 5265
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 4995 5179 1305
Size: 1230 2460 184 2007
Fragments arranged by size:
2460 2007 1230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2295 10
Size: 54 1425 343 463 3596
Fragments arranged by size:
3596 1425 463 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 2884 3839 4198 4357 518
Size: 648 1534 184 955 359 159 2042
Fragments arranged by size:
2042 1534 955 648 359 184 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2199 2399 2727 3297 3974 5122 5367
Size: 418 175 200 328 570 677 1148 245
Cuts at: 5367 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 570 418 328 245 200
175
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5329
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2038 2488 2598 2787 3168 3239 4784
Size: 97 809 450 110 189 381 71 1545
Cuts at: 4784 1132
Size: 2229
Fragments arranged by size:
2229 1545 809 450 381 189 110 97
71
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PfoI T'CCnGG_A
Cuts at: 2712 2712
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 2069
Size: 5881
PshAI GACnn'nnGTC
Cuts at: 1613 2765 1613
Size: 1152 4729
PsiI TTA'TAA
Cuts at: 2457 3451 2457
Size: 994 4887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2590 2622 2590
Size: 32 5849
PstI C_TGCA'G
Cuts at: 830 2029 3233 830
Size: 1199 1204 3478
Fragments arranged by size:
3478 1204 1199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2024 2199 2399 2727 2024
Size: 175 200 328 5178
Fragments arranged by size:
5178 328 200 175
SacI G_AGCT'C
Cuts at: 3024 3024
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SanDI GG'GwC_CC
Cuts at: 2069 2069
Size: 5881
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2025 3229 4364 5045 5236 826
Size: 219 980 1204 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 980 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3985 4886 5148 820
Size: 197 926 2042 901 262 1553
Fragments arranged by size:
2042 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3088 3805 5
Size: 47 3036 717 2081
Fragments arranged by size:
3036 2081 717 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2683 3320 513
Size: 552 1618 637 3074
Fragments arranged by size:
3074 1618 637 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3192 3908 3925 4078 4263 4708
Size: 1255 1509 103 716 17 153 185 445
Cuts at: 4708 325
Size: 1498
Fragments arranged by size:
1509 1498 1255 716 445 185 153 103
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 4127 5839
Size: 274 80 33 51 1443 54 2096 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
2096 1712 1443 274 138 80 54 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 2088 1316
Size: 772 5109
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI