Restriction map for
pFN21AE0247
(Circular) (Six-base) MAPSORT of: pFN21AE0247.seq Check: 3113 from: 1 to: 6556
pFN21AE0247 6556 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3488 3488
Size: 6556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2037 2094 2163 2622 2697 3099 3150
Size: 375 30 57 69 459 75 402 51
Cuts at: 3150 3325 4619 6003 6271 1632
Size: 175 1294 1384 268 1917
Fragments arranged by size:
1917 1384 1294 459 402 375 268 175
75 69 57 51 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 5455 6432 1203
Size: 4252 977 1327
Fragments arranged by size:
4252 1327 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 2479 2727 4299 5871 2479
Size: 248 1572 1572 3164
Fragments arranged by size:
3164 1572 1572 248
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2186 2299 2369 2734 2901 3477 3592 3865
Size: 693 113 70 365 167 576 115 273
Cuts at: 3865 4091 4243 6352 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 693 576 365 273 226 167
152 115 113 70
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BamHI G'GATC_C
Cuts at: 2680 3886 4253 2680
Size: 1206 367 4983
Fragments arranged by size:
4983 1206 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3877 5332 618
Size: 325 217 627 2090 1455 1842
Fragments arranged by size:
2090 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2667 3875 1391
Size: 1276 1208 4072
Fragments arranged by size:
4072 1276 1208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5585
BbvCI CC'TCA_GC
Cuts at: 2251 2499 3162 3433 2251
Size: 248 663 271 5374
Fragments arranged by size:
5374 663 271 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 2239 1548
Size: 691 5865
BfrBI ATG'CAT
Cuts at: 2425 2425
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2230 6551 2230
Size: 4321 2235
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 4617 5773 1790
Size: 2827 1156 2573
Fragments arranged by size:
2827 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2124 4287 5245
Size: 416 53 83 399 24 707 2163 958
Cuts at: 5245 442
Size: 1753
Fragments arranged by size:
2163 1753 958 707 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2109 2514 2109
Size: 405 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 5216 1239
Size: 1115 2862 2579
Fragments arranged by size:
2862 2579 1115
Bpu10I CC'TnA_GC
Cuts at: 2251 2499 3162 3433 2251
Size: 248 663 271 5374
Fragments arranged by size:
5374 663 271 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 4681 5546 5844 2612
Size: 2069 865 298 3324
Fragments arranged by size:
3324 2069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 2460 6550 2460
Size: 4090 2466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3337 3367 1178
Size: 30 165 30 1934 30 4367
Fragments arranged by size:
4367 1934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2983 3518 1186
Size: 1797 535 4224
Fragments arranged by size:
4224 1797 535
BseYI C'CCAG_C
Cuts at: 5759 6255 5759
Size: 496 6060
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3445 1163
Size: 573 1709 4274
Fragments arranged by size:
4274 1709 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4617 4702 5773 4617
Size: 85 1071 5400
Fragments arranged by size:
5400 1071 85
BsmI GAATG_Cn'
Cuts at: 2723 2815 3741 3808 4067 4160 2723
Size: 92 926 67 259 93 5119
Fragments arranged by size:
5119 926 259 93 92 67
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2667 3875 4617 4702 5773 1391
Size: 399 877 1208 742 85 1071 2174
Fragments arranged by size:
2174 1208 1071 877 742 399 85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 3082 4445 6175 3082
Size: 1363 1730 3463
Fragments arranged by size:
3463 1730 1363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 3488 3911 844
Size: 1471 1173 423 3489
Fragments arranged by size:
3489 1471 1173 423
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5051 5225 65
Size: 1183 3803 174 1396
Fragments arranged by size:
3803 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2261 2727 3812 794
Size: 1467 466 1085 3538
Fragments arranged by size:
3538 1467 1085 466
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 2230 2680 2839 3886 4253 4638 4655 6107
Size: 258 450 159 1047 367 385 17 1452
Cuts at: 6107 6193 6205 6551 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1047 450 385 367 346 258
159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3466 3466
Size: 6556
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5817
Fragments arranged by size:
5817 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3457 4072 4866 4886 2728
Size: 729 615 794 20 4398
Fragments arranged by size:
4398 794 729 615 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3715 3750 529
Size: 35 3151 35 3335
Fragments arranged by size:
3335 3151 35 35
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2983 4003 4892
Size: 54 1425 50 275 18 1153 1020 889
Cuts at: 4892 6521 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1153 1020 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 4486 1353
Size: 1957 1176 3423
Fragments arranged by size:
3423 1957 1176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2037 2094 2163 2354 2622 2697
Size: 393 375 30 57 69 191 268 75
Cuts at: 2697 3099 3150 3325 4619 5216 6003 6271 1239
Size: 402 51 175 1294 597 787 268 1524
Fragments arranged by size:
1524 1294 787 597 402 393 375 268
268 191 175 75 69 57 51 30
EcoO109I rG'GnC_Cy
Cuts at: 2362 2587 2362
Size: 225 6331
EcoRI G'AATT_C
Cuts at: 2186 2299 3592 3865 4243 2186
Size: 113 1293 273 378 4499
Fragments arranged by size:
4499 1293 378 273 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2234 2588 2234
Size: 354 6202
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3347 3379 3612 3644 5197
Size: 32 135 32 1775 32 233 32 1553
Cuts at: 5197 5229 5271 5303 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1775 1553 233 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3900 4146 669
Size: 988 281 1428 534 246 3079
Fragments arranged by size:
3079 1428 988 534 281 246
HindIII A'AGCT_T
Cuts at: 748 2550 748
Size: 1802 4754
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2291 5670 5854 1305
Size: 986 3379 184 2007
Fragments arranged by size:
3379 2007 986 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2985 10
Size: 54 1425 343 1153 3581
Fragments arranged by size:
3581 1425 1153 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2881 4514 4873 5032 518
Size: 648 1715 1633 359 159 2042
Fragments arranged by size:
2042 1715 1633 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2183 3972 4649 5797 6042 1606
Size: 577 1789 677 1148 245 2120
Fragments arranged by size:
2120 1789 1148 677 577 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6004
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NsiI A_TGCA'T
Cuts at: 2427 2427
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 3914 5459 1132
Size: 97 2685 1545 2229
Fragments arranged by size:
2685 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 5545
PciI A'CATG_T
Cuts at: 5455 5455
Size: 6556
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2587
Size: 6556
PshAI GACnn'nnGTC
Cuts at: 1613 2277 3576 1613
Size: 664 1299 4593
Fragments arranged by size:
4593 1299 664
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PstI C_TGCA'G
Cuts at: 830 3908 830
Size: 3078 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 2183 2183
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 4804 6516 4804
Size: 1712 4844
SfcI C'TryA_G
Cuts at: 826 1045 3324 3852 3904 5039 5720 5911 826
Size: 219 2279 528 52 1135 681 191 1471
Fragments arranged by size:
2279 1471 1135 681 528 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 4660 5561 5823 820
Size: 197 926 648 2069 901 262 1553
Fragments arranged by size:
2069 1553 926 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 3610 1517
Size: 2093 4463
StyI C'CwwG_G
Cuts at: 513 1065 3995 513
Size: 552 2930 3074
Fragments arranged by size:
3074 2930 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2372 3499 4583 4600 4753 4938 5383
Size: 1255 792 1127 1084 17 153 185 445
Cuts at: 5383 325
Size: 1498
Fragments arranged by size:
1498 1255 1127 1084 792 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4802 6514 96
Size: 274 80 33 51 1443 2825 1712 138
Fragments arranged by size:
2825 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3337 3369 6516 6548 3337
Size: 32 3147 32 3345
Fragments arranged by size:
3345 3147 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2061 1316
Size: 745 5811
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3559 3559
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 4685 4685
Size: 6556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI