Restriction map for
pFN21AE0242
(Circular) (Six-base) MAPSORT of: pFN21AE0242.seq Check: 1458 from: 1 to: 6061
pFN21AE0242 6061 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 3360 4188 4561 1563
Size: 1797 828 373 3063
Fragments arranged by size:
3063 1797 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2555 2753 4124 5508 5776 1632
Size: 801 122 198 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 801 268 198 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3195 1184
Size: 2011 4050
AlwNI CAG_nnn'CTG
Cuts at: 2117 2202 2243 2458 2528 3804 5376 2117
Size: 85 41 215 70 1276 1572 2802
Fragments arranged by size:
2802 1572 1276 215 85 70 41
ApaI G_GGCC'C
Cuts at: 2097 2470 2097
Size: 373 5688
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3370 3596 3748 5857 1493
Size: 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2988 3213 3386 1870
Size: 73 1045 225 173 4545
Fragments arranged by size:
4545 1045 225 173 73
BamHI G'GATC_C
Cuts at: 2680 3391 3758 2680
Size: 711 367 4983
Fragments arranged by size:
4983 711 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2583 2725 3255 3382 4837
Size: 325 217 627 796 142 530 127 1455
Cuts at: 4837 618
Size: 1842
Fragments arranged by size:
1842 1455 796 627 530 325 217 142
127
BanII G_rGCy'C
Cuts at: 1391 2097 2470 2664 2867 3062 3108 3222 3298
Size: 706 373 194 203 195 46 114 76
Cuts at: 3298 3380 1391
Size: 82 4072
Fragments arranged by size:
4072 706 373 203 195 194 114 82
76 46
BbeI G_GCGC'C
Cuts at: 2729 2729
Size: 6061
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 928
Size: 971 480 4610
Fragments arranged by size:
4610 971 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2885 2919 4252 4286 1903
Size: 34 50 34 864 34 1333 34 3678
Fragments arranged by size:
3678 1333 864 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 4672 136
Size: 107 122 71 631 605 1141 1859 1525
Fragments arranged by size:
1859 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 2312 3221 3478 2312
Size: 909 257 4895
Fragments arranged by size:
4895 909 257
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2470 2839 4122 5278 1790
Size: 307 373 369 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 373 369 307
BmgBI CAC'GTC
Cuts at: 1318 2434 2449 1318
Size: 1116 15 4930
Fragments arranged by size:
4930 1116 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2585 2717 3076 4721 1239
Size: 968 378 132 359 1645 2579
Fragments arranged by size:
2579 1645 968 378 359 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2208 4186 5051 5349 2208
Size: 1978 865 298 2920
Fragments arranged by size:
2920 1978 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 2296 4724 882
Size: 617 684 113 2428 2219
Fragments arranged by size:
2428 2219 684 617 113
BsaAI yAC'GTr
Cuts at: 493 1204 2482 3156 3852 493
Size: 711 1278 674 696 2702
Fragments arranged by size:
2702 1278 711 696 674
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2726 3021 3868 4250
Size: 53 83 186 936 1193 295 847 382
Cuts at: 4250 4885 275
Size: 635 1451
Fragments arranged by size:
1451 1193 936 847 635 382 295 186
83 53
BsaWI w'CCGG_w
Cuts at: 2773 2851 4494 4892 5166 5313 5724 2773
Size: 78 1643 398 274 147 411 3110
Fragments arranged by size:
3110 1643 411 398 274 147 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 1178
Size: 30 165 30 873 30 4933
Fragments arranged by size:
4933 873 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2381 2939 1186
Size: 1192 3 558 4308
Fragments arranged by size:
4308 1192 558 3
BseYI C'CCAG_C
Cuts at: 2202 3137 5264 5760 2202
Size: 935 2127 496 2503
Fragments arranged by size:
2503 2127 935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 2983 3155 3230 3257 1163
Size: 573 405 842 172 75 27 3967
Fragments arranged by size:
3967 842 573 405 172 75 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2773 3108 4122 4207 5278 2439
Size: 334 335 1014 85 1071 3222
Fragments arranged by size:
3222 1071 1014 335 334 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
BsmBI CGTCTCn'nnnn_
Cuts at: 2693 2693
Size: 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2439 2470 2664 2773 2839 2867
Size: 399 307 342 31 194 109 66 28
Cuts at: 2867 3062 3108 3222 3298 3380 4122 4207 5278
Size: 195 46 114 76 82 742 85 1071
Cuts at: 5278 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 342 307 195 194
114 109 85 82 76 66 46 31
28
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 3315 3416 844
Size: 1178 1293 101 3489
Fragments arranged by size:
3489 1293 1178 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2311 3948 1394
Size: 429 488 1637 3507
Fragments arranged by size:
3507 1637 488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3177 4556 4730 65
Size: 1183 1929 1379 174 1396
Fragments arranged by size:
1929 1396 1379 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 2737 3031 4705 4892 1700
Size: 110 528 399 294 1674 187 2869
Fragments arranged by size:
2869 1674 528 399 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 2894 4121 5133 2894
Size: 1227 1012 3822
Fragments arranged by size:
3822 1227 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2546 2356
Size: 190 5871
BstYI r'GATC_y
Cuts at: 1972 2113 2680 3004 3112 3391 3758 4143 4160
Size: 141 567 324 108 279 367 385 17
Cuts at: 4160 5612 5698 5710 6056 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 567 385 367 346 324 279
141 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2427 2427
Size: 6061
BtgI C'CryG_G
Cuts at: 513 1065 2539 2559 3323 513
Size: 552 1474 20 764 3251
Fragments arranged by size:
3251 1474 764 552 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2500 487
Size: 683 56 1274 4048
Fragments arranged by size:
4048 1274 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3142 3177 529
Size: 35 2578 35 3413
Fragments arranged by size:
3413 2578 35 35
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 2556 3508
Size: 54 1425 50 275 18 654 72 952
Cuts at: 3508 4397 6026 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 952 889 654 275 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2409 3327 3991 1353
Size: 1002 54 918 664 3423
Fragments arranged by size:
3423 1002 918 664 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3280 4648 5022 5168 387
Size: 1064 138 1691 1368 374 146 1280
Fragments arranged by size:
1691 1368 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3106 3106
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2433 2555 2585 2717 2753 3076
Size: 393 575 226 122 30 132 36 323
Cuts at: 3076 4124 4721 5508 5776 1239
Size: 1048 597 787 268 1524
Fragments arranged by size:
1524 1048 787 597 575 393 323 268
226 132 122 36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 2629 2954 2615
Size: 14 325 5722
Fragments arranged by size:
5722 325 14
EcoO109I rG'GnC_Cy
Cuts at: 2093 2423 2466 2534 2551 2654 2687 2825 2093
Size: 330 43 68 17 103 33 138 5329
Fragments arranged by size:
5329 330 138 103 68 43 33 17
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 2513 2907 4567 2513
Size: 394 1660 4007
Fragments arranged by size:
4007 1660 394
FspAI rTGC'GCAy
Cuts at: 2513 2513
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2729 2902 2948 3038 3170 5208 1678
Size: 1051 173 46 90 132 2038 2531
Fragments arranged by size:
2531 2038 1051 173 132 90 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 4702 4734 4776
Size: 32 135 32 670 32 2428 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 2428 670 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2725 2725
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2161 5175 5359 1305
Size: 856 3014 184 2007
Fragments arranged by size:
3014 2007 856 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 10
Size: 54 1425 343 654 3585
Fragments arranged by size:
3585 1425 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4019 4378 4537 518
Size: 648 2853 359 159 2042
Fragments arranged by size:
2853 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3082 3477 4154 5302 5547 1606
Size: 1476 395 677 1148 245 2120
Fragments arranged by size:
2120 1476 1148 677 395 245
NaeI GCC'GGC
Cuts at: 2340 2739 3033 2340
Size: 399 294 5368
Fragments arranged by size:
5368 399 294
NarI GG'CG_CC
Cuts at: 2726 2726
Size: 6061
NcoI C'CATG_G
Cuts at: 513 1065 3323 513
Size: 552 2258 3251
Fragments arranged by size:
3251 2258 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NgoMIV G'CCGG_C
Cuts at: 2338 2737 3031 2338
Size: 399 294 5368
Fragments arranged by size:
5368 399 294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2150 3010 1348
Size: 802 860 4399
Fragments arranged by size:
4399 860 802
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 2458 1108
Size: 918 432 4711
Fragments arranged by size:
4711 918 432
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 3156 3156
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2534 2551 2654 2534
Size: 17 103 5941
Fragments arranged by size:
5941 103 17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2093 2466 2093
Size: 373 5688
PspXI vC'TCGA_Gb
Cuts at: 2988 2988
Size: 6061
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2165 2197 2165
Size: 32 6029
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 3082 3082
Size: 6061
SacI G_AGCT'C
Cuts at: 3108 3108
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SanDI GG'GwC_CC
Cuts at: 2534 2551 2534
Size: 17 6044
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 3409 4544 5225 5416 826
Size: 219 2364 1135 681 191 1471
Fragments arranged by size:
2364 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2727 2727
Size: 6061
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3215 3388 1872
Size: 1343 173 4545
Fragments arranged by size:
4545 1343 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2223 2988 4165 5066 5328 820
Size: 197 926 280 765 1177 901 262 1553
Fragments arranged by size:
1553 1177 926 901 765 280 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 3323 3500 513
Size: 552 2258 177 3074
Fragments arranged by size:
3074 2258 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 3056 4088 4105 4258 4443 4888
Size: 1255 707 769 1032 17 153 185 445
Cuts at: 4888 325
Size: 1498
Fragments arranged by size:
1498 1255 1032 769 707 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 6021 6053 2276
Size: 32 3713 32 2284
Fragments arranged by size:
3713 2284 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 2988 1943
Size: 1045 5016
XmaI C'CCGG_G
Cuts at: 1870 3213 3386 1870
Size: 1343 173 4545
Fragments arranged by size:
4545 1343 173
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI
NruI NsiI PacI RsrII SacII SapI SexAI SfiI
SrfI SwaI XcmI