Restriction map for
pFN21AE0240
(Circular) (Six-base) MAPSORT of: pFN21AE0240.seq Check: 5827 from: 1 to: 6175
pFN21AE0240 6175 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 2045 3496 2045
Size: 1451 4724
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3316 4238 5622 5890 1632
Size: 1684 922 1384 268 1917
Fragments arranged by size:
1917 1684 1384 922 268
AfeI AGC'GCT
Cuts at: 3035 3035
Size: 6175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 2444 3134 3918 5490 2444
Size: 690 784 1572 3129
Fragments arranged by size:
3129 1572 784 690
ApaI G_GGCC'C
Cuts at: 2727 2796 3003 2727
Size: 69 207 5899
Fragments arranged by size:
5899 207 69
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2111 3484 3710 3862 5971 1493
Size: 618 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2612 3500 1870
Size: 73 390 279 888 4545
Fragments arranged by size:
4545 888 390 279 73
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2666 3496 4951 618
Size: 325 217 627 258 621 830 1455 1842
Fragments arranged by size:
1842 1455 830 627 621 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2360 2503 2727 2796 3003 3249 3494 1391
Size: 969 143 224 69 207 246 245 4072
Fragments arranged by size:
4072 969 246 245 224 207 143 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 928
Size: 971 990 4214
Fragments arranged by size:
4214 990 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3226 4067 5277 3226
Size: 841 1210 4124
Fragments arranged by size:
4124 1210 841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2459 4786 136
Size: 107 122 71 631 605 787 2327 1525
Fragments arranged by size:
2327 1525 787 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6170 6170
Size: 6175
BlpI GC'TnA_GC
Cuts at: 2359 3037 3592 2359
Size: 678 555 4942
Fragments arranged by size:
4942 678 555
Bme1580I G_kGCm'C
Cuts at: 1790 2483 2671 2706 2727 2796 3003 3189 4236
Size: 693 188 35 21 69 207 186 1047
Cuts at: 4236 5392 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1047 693 207 188 186 69
35 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2561 2693 3288
Size: 416 53 83 399 24 1144 132 595
Cuts at: 3288 3906 4864 442
Size: 618 958 1753
Fragments arranged by size:
1753 1144 958 618 595 416 399 132
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2219 2328 2466 2479 2515 2589 2876
Size: 821 159 109 138 13 36 74 287
Cuts at: 2876 4835 1239
Size: 1959 2579
Fragments arranged by size:
2579 1959 821 287 159 138 109 74
36 13
Bpu10I CC'TnA_GC
Cuts at: 2373 2373
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 2772 4838 882
Size: 617 696 577 2066 2219
Fragments arranged by size:
2219 2066 696 617 577
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2133 2178 4608 5006 5280 5427 5838 2133
Size: 45 2430 398 274 147 411 2470
Fragments arranged by size:
2470 2430 411 398 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2644 2674 1178
Size: 30 165 30 1241 30 4679
Fragments arranged by size:
4679 1241 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2735 2873 1186
Size: 1168 381 138 4488
Fragments arranged by size:
4488 1168 381 138
BseYI C'CCAG_C
Cuts at: 2468 2943 5378 5874 2468
Size: 475 2435 496 2769
Fragments arranged by size:
2769 2435 496 475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 2392 1163
Size: 573 467 189 4946
Fragments arranged by size:
4946 573 467 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2197 3307 4236 4321 5392 2197
Size: 1110 929 85 1071 2980
Fragments arranged by size:
2980 1110 1071 929 85
BsmI GAATG_Cn'
Cuts at: 3686 3779 3686
Size: 93 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2360 2483 2503 2671 2706 2727
Size: 399 407 163 123 20 168 35 21
Cuts at: 2727 2796 3003 3189 3249 3307 3494 4236 4321
Size: 69 207 186 60 58 187 742 85
Cuts at: 4321 5392 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 207 187 186
168 163 123 85 69 60 58 35
21 20
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 3187 3530 844
Size: 1972 371 343 3489
Fragments arranged by size:
3489 1972 371 343
BsrBI CCG'CTC
Cuts at: 1394 1823 2493 2529 2844 4062 1394
Size: 429 670 36 315 1218 3507
Fragments arranged by size:
3507 1218 670 429 315 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 4670 4844 65
Size: 1183 3422 174 1396
Fragments arranged by size:
3422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2966 4819 5006 1700
Size: 110 1156 1853 187 2869
Fragments arranged by size:
2869 1853 1156 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3153 794
Size: 2359 3816
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 2935 3316 1184
Size: 1751 381 4043
Fragments arranged by size:
4043 1751 381
BstXI CCAn_nnnn'nTGG
Cuts at: 2306 2644 2306
Size: 338 5837
BstYI r'GATC_y
Cuts at: 1972 2136 3171 3505 3872 4257 4274 5726 5812
Size: 164 1035 334 367 385 17 1452 86
Cuts at: 5812 5824 6170 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 334 164
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3216 3216
Size: 6175
BtgI C'CryG_G
Cuts at: 513 1065 2004 2637 3189 513
Size: 552 939 633 552 3499
Fragments arranged by size:
3499 939 633 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 3404 3433 3691 4485 4505 3404
Size: 29 258 794 20 5074
Fragments arranged by size:
5074 794 258 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2736 3622 4511
Size: 54 1425 50 275 18 906 886 889
Cuts at: 4511 6140 8
Size: 1629 43
Fragments arranged by size:
1629 1425 906 889 886 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3335 4105 1353
Size: 1982 770 3423
Fragments arranged by size:
3423 1982 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3009 4762 5136 5282 387
Size: 1064 138 1420 1753 374 146 1280
Fragments arranged by size:
1753 1420 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2219 2328 2466 2479 2515 2589
Size: 393 428 159 109 138 13 36 74
Cuts at: 2589 2876 3316 4238 4835 5622 5890 1239
Size: 287 440 922 597 787 268 1524
Fragments arranged by size:
1524 922 787 597 440 428 393 287
268 159 138 109 74 36 13
EcoO109I rG'GnC_Cy
Cuts at: 2330 2535 2595 2723 2724 2792 2793 2999 3459
Size: 205 60 128 1 68 1 206 460
Cuts at: 3459 2330
Size: 5046
Fragments arranged by size:
5046 460 206 205 128 68 60 1
1
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 3037 5322 1678
Size: 1359 2285 2531
Fragments arranged by size:
2531 2285 1359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373
Size: 32 135 32 3244 32 42 32 2626
Fragments arranged by size:
3244 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 2049 3500 2049
Size: 1451 4724
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5289 5473 1305
Size: 3984 184 2007
Fragments arranged by size:
3984 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 10
Size: 54 1425 343 906 3447
Fragments arranged by size:
3447 1425 906 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4133 4492 4651 518
Size: 648 2967 359 159 2042
Fragments arranged by size:
2967 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2854 3041 3167 3591 4268 5416 5661 1606
Size: 1248 187 126 424 677 1148 245 2120
Fragments arranged by size:
2120 1248 1148 677 424 245 187 126
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 6175
NcoI C'CATG_G
Cuts at: 513 1065 2004 2637 3189 513
Size: 552 939 633 552 3499
Fragments arranged by size:
3499 939 633 552 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 3533 5078 1132
Size: 97 2304 1545 2229
Fragments arranged by size:
2304 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2929 3277 1108
Size: 1821 348 4006
Fragments arranged by size:
4006 1821 348
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 2267 4244 4276 5757 5789 2235
Size: 32 1977 32 1481 32 2621
Fragments arranged by size:
2621 1977 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2330 2595 3459 2330
Size: 265 864 5046
Fragments arranged by size:
5046 864 265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PspOMI G'GGCC_C
Cuts at: 2723 2792 2999 2723
Size: 69 207 5899
Fragments arranged by size:
5899 207 69
PstI C_TGCA'G
Cuts at: 830 2329 3130 3166 3527 830
Size: 1499 801 36 361 3478
Fragments arranged by size:
3478 1499 801 361 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2854 3167 2854
Size: 313 5862
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2325 3126 3162 3523 4658 5339 5530
Size: 219 1280 801 36 361 1135 681 191
Cuts at: 5530 826
Size: 1471
Fragments arranged by size:
1471 1280 1135 801 681 361 219 191
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 2335 2614 3502 1872
Size: 463 279 888 4545
Fragments arranged by size:
4545 888 463 279
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4279 5180 5442 820
Size: 197 926 2336 901 262 1553
Fragments arranged by size:
2336 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2004 2637 3189 3373 3614 513
Size: 552 939 633 552 184 241 3074
Fragments arranged by size:
3074 939 633 552 552 241 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3040 3302 4202 4219 4372 4557 5002
Size: 1255 1460 262 900 17 153 185 445
Cuts at: 5002 325
Size: 1498
Fragments arranged by size:
1498 1460 1255 900 445 262 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2750 4421 6133
Size: 274 80 33 51 1443 773 1671 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
1712 1671 1443 773 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 2333 2612 3500 1870
Size: 463 279 888 4545
Fragments arranged by size:
4545 888 463 279
XmnI GAAnn'nnTTC
Cuts at: 2873 4304 2873
Size: 1431 4744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI