Restriction map for
pFN21AE0236
(Circular) (Six-base) MAPSORT of: pFN21AE0236.seq Check: 2446 from: 1 to: 5533
pFN21AE0236 5533 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 2396 3660 4033 1563
Size: 833 1264 373 3063
Fragments arranged by size:
3063 1264 833 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 3596 4980 5248 1632
Size: 865 1099 1384 268 1917
Fragments arranged by size:
1917 1384 1099 865 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 3276 4848 3276
Size: 1572 3961
ApaI G_GGCC'C
Cuts at: 2570 2570
Size: 5533
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2008 2842 3068 3220 5329 1493
Size: 515 834 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2858 1870
Size: 73 915 4545
Fragments arranged by size:
4545 915 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 2854 4309 618
Size: 325 217 627 630 437 1455 1842
Fragments arranged by size:
1842 1455 630 627 437 325 217
BanII G_rGCy'C
Cuts at: 1391 2382 2570 2852 1391
Size: 991 188 282 4072
Fragments arranged by size:
4072 991 282 188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2527 928
Size: 971 628 3934
Fragments arranged by size:
3934 971 628
BbvCI CC'TCA_GC
Cuts at: 2644 2644
Size: 5533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2950 2950
Size: 5533
Bme1580I G_kGCm'C
Cuts at: 1790 2420 2570 3594 4750 1790
Size: 630 150 1024 1156 2573
Fragments arranged by size:
2573 1156 1024 630 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 3264 4222
Size: 416 53 83 399 24 959 888 958
Cuts at: 4222 442
Size: 1753
Fragments arranged by size:
1753 959 958 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4193 1239
Size: 2954 2579
Bpu10I CC'TnA_GC
Cuts at: 2644 2644
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2552 4196 882
Size: 617 1053 1644 2219
Fragments arranged by size:
2219 1644 1053 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2352 3340 3722 4357
Size: 53 83 186 936 819 988 382 635
Cuts at: 4357 275
Size: 1451
Fragments arranged by size:
1451 988 936 819 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2647 1186
Size: 1099 362 4072
Fragments arranged by size:
4072 1099 362
BseYI C'CCAG_C
Cuts at: 2580 4736 5232 2580
Size: 2156 496 2881
Fragments arranged by size:
2881 2156 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3594 3679 4750 3594
Size: 85 1071 4377
Fragments arranged by size:
4377 1071 85
BsmI GAATG_Cn'
Cuts at: 2025 3044 3137 2025
Size: 1019 93 4421
Fragments arranged by size:
4421 1019 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2420 2570 2852 3594 3679 4750
Size: 399 592 38 150 282 742 85 1071
Cuts at: 4750 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 592 399 282 150 85
38
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 2313 2758 3422 5152 2313
Size: 445 664 1730 2694
Fragments arranged by size:
2694 1730 664 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2888 844
Size: 1406 638 3489
Fragments arranged by size:
3489 1406 638
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 2392 4337 2392
Size: 1945 3588
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2279 2279
Size: 5533
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4981
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2461 487
Size: 683 56 1235 3559
Fragments arranged by size:
3559 1235 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 3843 3863 3049
Size: 794 20 4719
Fragments arranged by size:
4719 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2410 2837 2410
Size: 427 5106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2195 4540 809
Size: 507 879 2345 1802
Fragments arranged by size:
2345 1802 879 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2980 3869
Size: 54 1425 50 275 18 411 739 889
Cuts at: 3869 5498 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 411 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2453 2516 3463 1353
Size: 1041 59 63 947 3423
Fragments arranged by size:
3423 1041 947 63 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2236 4120 4494 4640 387
Size: 1064 138 647 1884 374 146 1280
Fragments arranged by size:
1884 1280 1064 647 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 3596 4193 4980 5248 1239
Size: 393 865 1099 597 787 268 1524
Fragments arranged by size:
1524 1099 865 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2197 2197
Size: 5533
EcoRI G'AATT_C
Cuts at: 2008 2842 3220 2008
Size: 834 378 4321
Fragments arranged by size:
4321 834 378
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 4680 1678
Size: 3002 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4174 4206 4248 4280 1373
Size: 32 135 32 2602 32 42 32 2626
Fragments arranged by size:
2626 2602 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3711
Fragments arranged by size:
3711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 3491 3850 4009 518
Size: 648 1179 1146 359 159 2042
Fragments arranged by size:
2042 1179 1146 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2949 3626 4774 5019 1606
Size: 1343 677 1148 245 2120
Fragments arranged by size:
2120 1343 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4981
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2456 2891 4436 1132
Size: 97 1227 435 1545 2229
Fragments arranged by size:
2229 1545 1227 435 97
PasI CC'CwG_GG
Cuts at: 1348 2563 1348
Size: 1215 4318
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2199 2490 2199
Size: 291 5242
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2085 2117 3602 3634 5115 5147 2085
Size: 32 1485 32 1481 32 2471
Fragments arranged by size:
2471 1485 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2197
Size: 5533
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 2141 3103 2141
Size: 962 4571
PspOMI G'GGCC_C
Cuts at: 2566 2566
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2885 830
Size: 2055 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 2453
Size: 5533
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SexAI A'CCwGG_T
Cuts at: 2529 2529
Size: 5533
SfcI C'TryA_G
Cuts at: 826 1045 2881 4016 4697 4888 826
Size: 219 1836 1135 681 191 1471
Fragments arranged by size:
1836 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 2719 2810 3457 5
Size: 47 2667 91 647 2081
Fragments arranged by size:
2667 2081 647 91 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2972 513
Size: 552 1907 3074
Fragments arranged by size:
3074 1907 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 3560 3577 3730 3915 4360 325
Size: 1255 876 1104 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1104 876 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 2094 2546 3662 2094
Size: 452 1116 3965
Fragments arranged by size:
3965 1116 452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI