Restriction map for
pFN21AE0227
(Circular) (Six-base) MAPSORT of: pFN21AE0227.seq Check: 3630 from: 1 to: 5035
pFN21AE0227 5035 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2845 278
Size: 53 83 186 936 1309 2468
Fragments arranged by size:
2468 1309 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2378 1937
Size: 441 4594
Acc65I G'GTAC_C
Cuts at: 2356 2356
Size: 5035
AclI AA'CG_TT
Cuts at: 1563 3162 3535 1563
Size: 1599 373 3063
Fragments arranged by size:
3063 1599 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3098 4482 4750 1632
Size: 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4838
AflIII A'CryG_T
Cuts at: 1203 3934 4911 1203
Size: 2731 977 1327
Fragments arranged by size:
2731 1327 977
AgeI A'CCGG_T
Cuts at: 3866 3866
Size: 5035
AhdI GACnn_n'nnGTC
Cuts at: 3764 3764
Size: 5035
AleI CACnn'nnGTG
Cuts at: 1166 2083 1166
Size: 917 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5035
AlwNI CAG_nnn'CTG
Cuts at: 2778 4350 2778
Size: 1572 3463
ApaLI G'TGCA_C
Cuts at: 3092 4248 3092
Size: 1156 3879
ApoI r'AATT_y
Cuts at: 1493 2344 2570 2722 4831 1493
Size: 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 851 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3589 160
Size: 872 2557 1606
Fragments arranged by size:
2557 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5035
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 1870
Size: 73 417 4545
Fragments arranged by size:
4545 417 73
BamHI G'GATC_C
Cuts at: 2365 2732 2365
Size: 367 4668
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2259 2356 3811 618
Size: 325 217 627 466 6 97 1455 1842
Fragments arranged by size:
1842 1455 627 466 325 217 97 6
BanII G_rGCy'C
Cuts at: 1391 2270 2303 2354 1391
Size: 879 33 51 4072
Fragments arranged by size:
4072 879 51 33
BbeI G_GCGC'C
Cuts at: 2257 2257
Size: 5035
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3226 3260 1903
Size: 34 1289 34 3678
Fragments arranged by size:
3678 1289 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2927 4137 2927
Size: 1210 3825
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5035
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3646 136
Size: 107 122 71 631 605 1974 1525
Fragments arranged by size:
1974 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5030 5030
Size: 5035
BlpI GC'TnA_GC
Cuts at: 2452 2452
Size: 5035
Bme1580I G_kGCm'C
Cuts at: 1790 2114 3096 4252 1790
Size: 324 982 1156 2573
Fragments arranged by size:
2573 1156 982 324
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5035
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2766 3724
Size: 416 53 83 399 24 684 665 958
Cuts at: 3724 442
Size: 1753
Fragments arranged by size:
1753 958 684 665 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3695 1239
Size: 2456 2579
Bpu10I CC'TnA_GC
Cuts at: 2222 2222
Size: 5035
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3160 4025 4323 3160
Size: 865 298 3872
Fragments arranged by size:
3872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3698 882
Size: 617 2199 2219
Fragments arranged by size:
2219 2199 617
BsaAI yAC'GTr
Cuts at: 493 1204 2826 493
Size: 711 1622 2702
Fragments arranged by size:
2702 1622 711
BsaBI GATnn'nnATC
Cuts at: 5029 5029
Size: 5035
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2254 2842 3224 3859
Size: 53 83 186 936 721 588 382 635
Cuts at: 3859 275
Size: 1451
Fragments arranged by size:
1451 936 721 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3468 3866 4140 4287 4698 3468
Size: 398 274 147 411 3805
Fragments arranged by size:
3805 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4810
Fragments arranged by size:
4810 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5035
BseYI C'CCAG_C
Cuts at: 2113 2177 4238 4734 2113
Size: 64 2061 496 2414
Fragments arranged by size:
2414 2061 496 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2485 3246 4274 1540
Size: 275 186 484 761 1028 2301
Fragments arranged by size:
2301 1028 761 484 275 186
BsiHKAI G_wGCw'C
Cuts at: 3096 3181 4252 3096
Size: 85 1071 3879
Fragments arranged by size:
3879 1071 85
BsmI GAATG_Cn'
Cuts at: 2546 2639 2546
Size: 93 4942
BsmBI CGTCTCn'nnnn_
Cuts at: 2246 2246
Size: 5035
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2270 2303 2354 3096 3181 4252
Size: 399 324 156 33 51 742 85 1071
Cuts at: 4252 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 324 156 85 51
33
BspEI T'CCGG_A
Cuts at: 4698 4698
Size: 5035
BspHI T'CATG_A
Cuts at: 2924 4654 2924
Size: 1730 3305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 844
Size: 1546 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2922 1394
Size: 429 1099 3507
Fragments arranged by size:
3507 1099 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3530 3704 65
Size: 1183 2282 174 1396
Fragments arranged by size:
2282 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2250 2256 3679 3866 1700
Size: 110 440 6 1423 187 2869
Fragments arranged by size:
2869 1423 440 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5035
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5035
BssSI C'ACGA_G
Cuts at: 3095 4107 3095
Size: 1012 4023
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5035
BstBI TT'CG_AA
Cuts at: 3839 3839
Size: 5035
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5035
BstXI CCAn_nnnn'nTGG
Cuts at: 2321 2321
Size: 5035
BstYI r'GATC_y
Cuts at: 1972 2365 2732 3117 3134 4586 4672 4684 5030
Size: 393 367 385 17 1452 86 12 346
Cuts at: 5030 1972
Size: 1977
Fragments arranged by size:
1977 1452 393 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4296
Fragments arranged by size:
4296 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 2551 3345 3365 2036
Size: 515 794 20 3706
Fragments arranged by size:
3706 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5035
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5000
DraI TTT'AAA
Cuts at: 2339 2339
Size: 5035
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4042 809
Size: 507 2726 1802
Fragments arranged by size:
2726 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 3371 5000
Size: 54 1425 50 275 18 652 889 1629
Cuts at: 5000 8
Size: 43
Fragments arranged by size:
1629 1425 889 652 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2482 1537
Size: 275 670 4090
Fragments arranged by size:
4090 670 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 2249 2965 1353
Size: 790 106 716 3423
Fragments arranged by size:
3423 790 716 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3622 3996 4142 387
Size: 1064 138 2033 374 146 1280
Fragments arranged by size:
2033 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3098 3695 4482 4750 1239
Size: 393 1466 597 787 268 1524
Fragments arranged by size:
1524 1466 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2235 2235
Size: 5035
EcoRI G'AATT_C
Cuts at: 2344 2722 2344
Size: 378 4657
FspI TGC'GCA
Cuts at: 3541 3541
Size: 5035
HaeII r_GCGC'y
Cuts at: 1678 2257 4182 1678
Size: 579 1925 2531
Fragments arranged by size:
2531 1925 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3676 3708 3750 3782 1373
Size: 32 135 32 2104 32 42 32 2626
Fragments arranged by size:
2626 2104 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2379 2625 669
Size: 988 281 441 246 3079
Fragments arranged by size:
3079 988 441 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5035
HpaI GTT'AAC
Cuts at: 2625 2625
Size: 5035
KasI G'GCGC_C
Cuts at: 2253 2253
Size: 5035
KpnI G_GTAC'C
Cuts at: 2360 2360
Size: 5035
MfeI C'AATT_G
Cuts at: 2634 2634
Size: 5035
MluI A'CGCG_T
Cuts at: 4911 4911
Size: 5035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4149 4333 1305
Size: 2844 184 2007
Fragments arranged by size:
2844 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3213
Fragments arranged by size:
3213 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2083 2993 3352 3511 518
Size: 648 917 910 359 159 2042
Fragments arranged by size:
2042 917 910 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2451 3128 4276 4521 1606
Size: 845 677 1148 245 2120
Fragments arranged by size:
2120 1148 845 677 245
NaeI GCC'GGC
Cuts at: 2258 2258
Size: 5035
NarI GG'CG_CC
Cuts at: 2254 2254
Size: 5035
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5035
NgoMIV G'CCGG_C
Cuts at: 2256 2256
Size: 5035
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5035
NotI GC'GGCC_GC
Cuts at: 2482 2482
Size: 5035
NspI r_CATG'y
Cuts at: 1132 1229 2393 3938 1132
Size: 97 1164 1545 2229
Fragments arranged by size:
2229 1545 1164 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5035
PciI A'CATG_T
Cuts at: 3934 3934
Size: 5035
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5035
PmeI GTTT'AAAC
Cuts at: 2339 2339
Size: 5035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 4617 4649 3104
Size: 32 1481 32 3490
Fragments arranged by size:
3490 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5035
PsiI TTA'TAA
Cuts at: 2605 2605
Size: 5035
PstI C_TGCA'G
Cuts at: 830 2134 2387 830
Size: 1304 253 3478
Fragments arranged by size:
3478 1304 253
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5035
SalI G'TCGA_C
Cuts at: 1936 2377 1936
Size: 441 4594
SbfI CC_TGCA'GG
Cuts at: 2387 2387
Size: 5035
ScaI AGT'ACT
Cuts at: 3283 4995 3283
Size: 1712 3323
SfcI C'TryA_G
Cuts at: 826 1045 2130 2383 3518 4199 4390 826
Size: 219 1085 253 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 681 253 219 191
SfoI GGC'GCC
Cuts at: 2255 2255
Size: 5035
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5035
SgrAI Cr'CCGG_yG
Cuts at: 1700 2250 1700
Size: 550 4485
SmaI CCC'GGG
Cuts at: 1872 2362 1872
Size: 490 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3139 4040 4302 820
Size: 197 926 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2826 493
Size: 2333 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5035
SphI G_CATG'C
Cuts at: 1132 2393 1132
Size: 1261 3774
SspI AAT'ATT
Cuts at: 5 52 2959 5
Size: 47 2907 2081
Fragments arranged by size:
2907 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5035
StyI C'CwwG_G
Cuts at: 513 1065 2474 513
Size: 552 1409 3074
Fragments arranged by size:
3074 1409 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3079 3232 3417 3862 325
Size: 1255 1482 17 153 185 445 1498
Fragments arranged by size:
1498 1482 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3281 4993 96
Size: 274 80 33 51 1443 1304 1712 138
Fragments arranged by size:
1712 1443 1304 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4995 5027 4995
Size: 32 5003
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5035
XbaI T'CTAG_A
Cuts at: 2371 2371
Size: 5035
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5035
XmaI C'CCGG_G
Cuts at: 1870 2360 1870
Size: 490 4545
XmnI GAAnn'nnTTC
Cuts at: 3164 3164
Size: 5035
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2843 276
Size: 53 83 186 936 1309 2468
Fragments arranged by size:
2468 1309 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI