Restriction map for
pFN21AE0225
(Circular) (Six-base) MAPSORT of: pFN21AE0225.seq Check: 8099 from: 1 to: 5059
pFN21AE0225 5059 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2869 278
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2402 1937
Size: 465 4594
Acc65I G'GTAC_C
Cuts at: 2380 2380
Size: 5059
AclI AA'CG_TT
Cuts at: 1563 3186 3559 1563
Size: 1623 373 3063
Fragments arranged by size:
3063 1623 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3122 4506 4774 1632
Size: 1490 1384 268 1917
Fragments arranged by size:
1917 1490 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4862
AflIII A'CryG_T
Cuts at: 1203 3958 4935 1203
Size: 2755 977 1327
Fragments arranged by size:
2755 1327 977
AgeI A'CCGG_T
Cuts at: 3890 3890
Size: 5059
AhdI GACnn_n'nnGTC
Cuts at: 3788 3788
Size: 5059
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5059
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5059
AlwNI CAG_nnn'CTG
Cuts at: 2265 2307 2802 4374 2265
Size: 42 495 1572 2950
Fragments arranged by size:
2950 1572 495 42
ApaLI G'TGCA_C
Cuts at: 3116 4272 3116
Size: 1156 3903
ApoI r'AATT_y
Cuts at: 1493 2368 2594 2746 4855 1493
Size: 875 226 152 2109 1697
Fragments arranged by size:
2109 1697 875 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3613 160
Size: 872 2581 1606
Fragments arranged by size:
2581 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
AvaI C'yCGr_G
Cuts at: 1870 1943 2384 1870
Size: 73 441 4545
Fragments arranged by size:
4545 441 73
BamHI G'GATC_C
Cuts at: 2389 2756 2389
Size: 367 4692
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2380 3835 618
Size: 325 217 627 593 1455 1842
Fragments arranged by size:
1842 1455 627 593 325 217
BanII G_rGCy'C
Cuts at: 1391 2300 2327 2378 1391
Size: 909 27 51 4072
Fragments arranged by size:
4072 909 51 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3250 3284 1903
Size: 34 1313 34 3678
Fragments arranged by size:
3678 1313 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2951 4161 2951
Size: 1210 3849
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5059
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3670 136
Size: 107 122 71 631 605 1998 1525
Fragments arranged by size:
1998 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5054 5054
Size: 5059
BlpI GC'TnA_GC
Cuts at: 2476 2476
Size: 5059
Bme1580I G_kGCm'C
Cuts at: 1790 3120 4276 1790
Size: 1330 1156 2573
Fragments arranged by size:
2573 1330 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2113 2790 3748
Size: 416 53 83 399 24 696 677 958
Cuts at: 3748 442
Size: 1753
Fragments arranged by size:
1753 958 696 677 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2284 2367 3719 1239
Size: 888 157 83 1352 2579
Fragments arranged by size:
2579 1352 888 157 83
Bpu10I CC'TnA_GC
Cuts at: 2210 2210
Size: 5059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3184 4049 4347 3184
Size: 865 298 3896
Fragments arranged by size:
3896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3722 882
Size: 617 2223 2219
Fragments arranged by size:
2223 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2850 493
Size: 711 1646 2702
Fragments arranged by size:
2702 1646 711
BsaBI GATnn'nnATC
Cuts at: 5053 5053
Size: 5059
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2242 2866 3248 3883
Size: 53 83 186 936 709 624 382 635
Cuts at: 3883 275
Size: 1451
Fragments arranged by size:
1451 936 709 635 624 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3492 3890 4164 4311 4722 3492
Size: 398 274 147 411 3829
Fragments arranged by size:
3829 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4834
Fragments arranged by size:
4834 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 1186
Size: 845 4214
BseYI C'CCAG_C
Cuts at: 4262 4758 4262
Size: 496 4563
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2509 3270 4298 1540
Size: 275 186 508 761 1028 2301
Fragments arranged by size:
2301 1028 761 508 275 186
BsiHKAI G_wGCw'C
Cuts at: 3120 3205 4276 3120
Size: 85 1071 3903
Fragments arranged by size:
3903 1071 85
BsmI GAATG_Cn'
Cuts at: 2570 2663 2570
Size: 93 4966
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2327 2378 3120 3205 4276 1391
Size: 399 510 27 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 85 51 27
BspEI T'CCGG_A
Cuts at: 4722 4722
Size: 5059
BspHI T'CATG_A
Cuts at: 2948 4678 2948
Size: 1730 3329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 844
Size: 1570 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2946 1394
Size: 429 1123 3507
Fragments arranged by size:
3507 1123 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3554 3728 65
Size: 1183 2306 174 1396
Fragments arranged by size:
2306 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3703 3890 1700
Size: 110 1893 187 2869
Fragments arranged by size:
2869 1893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5059
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5059
BssSI C'ACGA_G
Cuts at: 3119 4131 3119
Size: 1012 4047
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5059
BstBI TT'CG_AA
Cuts at: 3863 3863
Size: 5059
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5059
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 2318 2345 2291
Size: 27 27 5005
Fragments arranged by size:
5005 27 27
BstYI r'GATC_y
Cuts at: 1972 2389 2756 3141 3158 4610 4696 4708 5054
Size: 417 367 385 17 1452 86 12 346
Cuts at: 5054 1972
Size: 1977
Fragments arranged by size:
1977 1452 417 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4320
Fragments arranged by size:
4320 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 2278 2284 2575 3369 3389 2036
Size: 242 6 291 794 20 3706
Fragments arranged by size:
3706 794 291 242 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5024
DraI TTT'AAA
Cuts at: 2363 2363
Size: 5059
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4066 809
Size: 507 2750 1802
Fragments arranged by size:
2750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2281 2506 3395
Size: 54 1425 50 275 18 451 225 889
Cuts at: 3395 5024 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 451 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2506 1537
Size: 275 694 4090
Fragments arranged by size:
4090 694 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2155 2989 1353
Size: 802 834 3423
Fragments arranged by size:
3423 834 802
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3646 4020 4166 387
Size: 1064 138 2057 374 146 1280
Fragments arranged by size:
2057 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2284 2367 3122 3719 4506 4774
Size: 393 495 157 83 755 597 787 268
Cuts at: 4774 1239
Size: 1524
Fragments arranged by size:
1524 787 755 597 495 393 268 157
83
EcoO109I rG'GnC_Cy
Cuts at: 2223 2223
Size: 5059
EcoRI G'AATT_C
Cuts at: 2368 2746 2368
Size: 378 4681
FspI TGC'GCA
Cuts at: 3565 3565
Size: 5059
HaeII r_GCGC'y
Cuts at: 1678 4206 1678
Size: 2528 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3700 3732 3774 3806 1373
Size: 32 135 32 2128 32 42 32 2626
Fragments arranged by size:
2626 2128 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2403 2649 669
Size: 988 281 465 246 3079
Fragments arranged by size:
3079 988 465 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5059
HpaI GTT'AAC
Cuts at: 2649 2649
Size: 5059
KpnI G_GTAC'C
Cuts at: 2384 2384
Size: 5059
MfeI C'AATT_G
Cuts at: 2658 2658
Size: 5059
MluI A'CGCG_T
Cuts at: 4935 4935
Size: 5059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 4173 4357 1305
Size: 958 1910 184 2007
Fragments arranged by size:
2007 1910 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 10
Size: 54 1425 343 451 2786
Fragments arranged by size:
2786 1425 451 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3376 3535 518
Size: 648 1851 359 159 2042
Fragments arranged by size:
2042 1851 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2262 2304 2475 3152 4300 4545 1606
Size: 656 42 171 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 656 245 171 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4507
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5059
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5059
NotI GC'GGCC_GC
Cuts at: 2506 2506
Size: 5059
NspI r_CATG'y
Cuts at: 1132 1229 2417 3962 1132
Size: 97 1188 1545 2229
Fragments arranged by size:
2229 1545 1188 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5059
PciI A'CATG_T
Cuts at: 3958 3958
Size: 5059
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5059
PmeI GTTT'AAAC
Cuts at: 2363 2363
Size: 5059
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 4641 4673 3128
Size: 32 1481 32 3514
Fragments arranged by size:
3514 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5059
PsiI TTA'TAA
Cuts at: 2629 2629
Size: 5059
PstI C_TGCA'G
Cuts at: 830 2186 2411 830
Size: 1356 225 3478
Fragments arranged by size:
3478 1356 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5059
PvuII CAG'CTG
Cuts at: 2262 2304 2262
Size: 42 5017
SalI G'TCGA_C
Cuts at: 1936 2401 1936
Size: 465 4594
SbfI CC_TGCA'GG
Cuts at: 2411 2411
Size: 5059
ScaI AGT'ACT
Cuts at: 3307 5019 3307
Size: 1712 3347
SfcI C'TryA_G
Cuts at: 826 1045 2182 2407 3542 4223 4414 826
Size: 219 1137 225 1135 681 191 1471
Fragments arranged by size:
1471 1137 1135 681 225 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5059
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5059
SmaI CCC'GGG
Cuts at: 1872 2386 1872
Size: 514 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3163 4064 4326 820
Size: 197 926 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2850 493
Size: 2357 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5059
SphI G_CATG'C
Cuts at: 1132 2417 1132
Size: 1285 3774
SspI AAT'ATT
Cuts at: 5 52 2983 5
Size: 47 2931 2081
Fragments arranged by size:
2931 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5059
StyI C'CwwG_G
Cuts at: 513 1065 2498 513
Size: 552 1433 3074
Fragments arranged by size:
3074 1433 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3103 3256 3441 3886 325
Size: 1255 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2075 3305 5017
Size: 274 80 33 51 1443 98 1230 1712
Cuts at: 5017 96
Size: 138
Fragments arranged by size:
1712 1443 1230 274 138 98 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5019 5051 5019
Size: 32 5027
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5059
XbaI T'CTAG_A
Cuts at: 2395 2395
Size: 5059
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5059
XmaI C'CCGG_G
Cuts at: 1870 2384 1870
Size: 514 4545
XmnI GAAnn'nnTTC
Cuts at: 3188 3188
Size: 5059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2867 276
Size: 53 83 186 936 1333 2468
Fragments arranged by size:
2468 1333 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI