Restriction map for
pFN21AE0215
(Circular) (Six-base) MAPSORT of: pFN21AE0215.seq Check: 3929 from: 1 to: 5038
pFN21AE0215 5038 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2848 278
Size: 53 83 186 936 1312 2468
Fragments arranged by size:
2468 1312 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2328 2381 1937
Size: 391 53 4594
Fragments arranged by size:
4594 391 53
Acc65I G'GTAC_C
Cuts at: 2235 2359 2235
Size: 124 4914
AclI AA'CG_TT
Cuts at: 1563 3165 3538 1563
Size: 1602 373 3063
Fragments arranged by size:
3063 1602 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 4485 4753 1632
Size: 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 268
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 5038
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4841
AflIII A'CryG_T
Cuts at: 1203 3937 4914 1203
Size: 2734 977 1327
Fragments arranged by size:
2734 1327 977
AgeI A'CCGG_T
Cuts at: 3869 3869
Size: 5038
AhdI GACnn_n'nnGTC
Cuts at: 3767 3767
Size: 5038
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5038
AlwNI CAG_nnn'CTG
Cuts at: 2781 4353 2781
Size: 1572 3466
ApaLI G'TGCA_C
Cuts at: 3095 4251 3095
Size: 1156 3882
ApoI r'AATT_y
Cuts at: 1493 2347 2573 2725 4834 1493
Size: 854 226 152 2109 1697
Fragments arranged by size:
2109 1697 854 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3592 160
Size: 872 2560 1606
Fragments arranged by size:
2560 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5038
AvaI C'yCGr_G
Cuts at: 1870 1943 2363 1870
Size: 73 420 4545
Fragments arranged by size:
4545 420 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2227 2260 2227
Size: 33 5005
BamHI G'GATC_C
Cuts at: 2368 2735 2368
Size: 367 4671
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2359 3814 618
Size: 325 217 627 448 124 1455 1842
Fragments arranged by size:
1842 1455 627 448 325 217 124
BanII G_rGCy'C
Cuts at: 1391 2357 1391
Size: 966 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3229 3263 1903
Size: 34 1292 34 3678
Fragments arranged by size:
3678 1292 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2930 4140 2930
Size: 1210 3828
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5038
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3649 136
Size: 107 122 71 631 605 1977 1525
Fragments arranged by size:
1977 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5033 5033
Size: 5038
BlpI GC'TnA_GC
Cuts at: 2455 2455
Size: 5038
Bme1580I G_kGCm'C
Cuts at: 1790 3099 4255 1790
Size: 1309 1156 2573
Fragments arranged by size:
2573 1309 1156
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 4201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2769 3727 442
Size: 416 53 83 399 24 1352 958 1753
Fragments arranged by size:
1753 1352 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3698 1239
Size: 2459 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3163 4028 4326 3163
Size: 865 298 3875
Fragments arranged by size:
3875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3701 882
Size: 617 2202 2219
Fragments arranged by size:
2219 2202 617
BsaAI yAC'GTr
Cuts at: 493 1204 2829 493
Size: 711 1625 2702
Fragments arranged by size:
2702 1625 711
BsaBI GATnn'nnATC
Cuts at: 2207 5032 2207
Size: 2825 2213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2845 3227 3862 275
Size: 53 83 186 936 1312 382 635 1451
Fragments arranged by size:
1451 1312 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2032 3471 3869 4143 4290 4701 2032
Size: 1439 398 274 147 411 2369
Fragments arranged by size:
2369 1439 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4813
Fragments arranged by size:
4813 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4014
BseYI C'CCAG_C
Cuts at: 4241 4737 4241
Size: 496 4542
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 1163
Size: 573 507 3958
Fragments arranged by size:
3958 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2488 3249 4277 1540
Size: 275 186 57 430 761 1028 2301
Fragments arranged by size:
2301 1028 761 430 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2123 2253 2279 3099 3184 4255 2123
Size: 130 26 820 85 1071 2906
Fragments arranged by size:
2906 1071 820 130 85 26
BsmI GAATG_Cn'
Cuts at: 2549 2642 2549
Size: 93 4945
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2253 2279 2357 3099 3184 4255
Size: 399 333 130 26 78 742 85 1071
Cuts at: 4255 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 333 130 85 78
26
BspEI T'CCGG_A
Cuts at: 4701 4701
Size: 5038
BspHI T'CATG_A
Cuts at: 2927 4657 2927
Size: 1730 3308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 844
Size: 1549 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2925 1394
Size: 429 1102 3507
Fragments arranged by size:
3507 1102 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3533 3707 65
Size: 1183 2285 174 1396
Fragments arranged by size:
2285 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3682 3869 1700
Size: 110 1872 187 2869
Fragments arranged by size:
2869 1872 187 110
BsrGI T'GTAC_A
Cuts at: 96 2085 96
Size: 1989 3049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5038
BssSI C'ACGA_G
Cuts at: 3098 4110 3098
Size: 1012 4026
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2257 794
Size: 1463 3575
BstBI TT'CG_AA
Cuts at: 3842 3842
Size: 5038
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5038
BstYI r'GATC_y
Cuts at: 1972 2139 2368 2735 3120 3137 4589 4675 4687
Size: 167 229 367 385 17 1452 86 12
Cuts at: 4687 5033 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 229 167 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4299
Fragments arranged by size:
4299 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2251 2554 3348 3368 2188
Size: 63 303 794 20 3858
Fragments arranged by size:
3858 794 303 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5003
DraI TTT'AAA
Cuts at: 2342 2342
Size: 5038
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4045 809
Size: 507 2729 1802
Fragments arranged by size:
2729 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2485 3374
Size: 54 1425 50 275 18 225 430 889
Cuts at: 3374 5003 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 430 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2485 1537
Size: 275 243 430 4090
Fragments arranged by size:
4090 430 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2968 1353
Size: 1615 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3625 3999 4145 387
Size: 1064 138 2036 374 146 1280
Fragments arranged by size:
2036 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3101 3698 4485 4753 1239
Size: 393 1469 597 787 268 1524
Fragments arranged by size:
1524 1469 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2132 2132
Size: 5038
EcoRI G'AATT_C
Cuts at: 2347 2725 2347
Size: 378 4660
FspI TGC'GCA
Cuts at: 3544 3544
Size: 5038
HaeII r_GCGC'y
Cuts at: 1678 2084 2287 4185 1678
Size: 406 203 1898 2531
Fragments arranged by size:
2531 1898 406 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 3679 3711 3753
Size: 32 135 32 552 32 1523 32 42
Cuts at: 3753 3785 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 552 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2382 2628 669
Size: 988 281 444 246 3079
Fragments arranged by size:
3079 988 444 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5038
HpaI GTT'AAC
Cuts at: 2628 2628
Size: 5038
KpnI G_GTAC'C
Cuts at: 2239 2363 2239
Size: 124 4914
MfeI C'AATT_G
Cuts at: 2637 2637
Size: 5038
MluI A'CGCG_T
Cuts at: 4914 4914
Size: 5038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4152 4336 1305
Size: 2847 184 2007
Fragments arranged by size:
2847 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3216
Fragments arranged by size:
3216 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3355 3514 518
Size: 648 1830 359 159 2042
Fragments arranged by size:
2042 1830 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2454 3131 4279 4524 1606
Size: 848 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5038
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5038
NotI GC'GGCC_GC
Cuts at: 2055 2485 2055
Size: 430 4608
NspI r_CATG'y
Cuts at: 1132 1229 2396 3941 1132
Size: 97 1167 1545 2229
Fragments arranged by size:
2229 1545 1167 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5038
PciI A'CATG_T
Cuts at: 3937 3937
Size: 5038
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5038
PfoI T'CCnGG_A
Cuts at: 2123 2123
Size: 5038
PmeI GTTT'AAAC
Cuts at: 2342 2342
Size: 5038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 4620 4652 3107
Size: 32 1481 32 3493
Fragments arranged by size:
3493 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2132 2132
Size: 5038
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5038
PsiI TTA'TAA
Cuts at: 2608 2608
Size: 5038
PstI C_TGCA'G
Cuts at: 830 2274 2390 830
Size: 1444 116 3478
Fragments arranged by size:
3478 1444 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5038
SalI G'TCGA_C
Cuts at: 1936 2380 1936
Size: 444 4594
SanDI GG'GwC_CC
Cuts at: 2132 2132
Size: 5038
SbfI CC_TGCA'GG
Cuts at: 2390 2390
Size: 5038
ScaI AGT'ACT
Cuts at: 3286 4998 3286
Size: 1712 3326
SfcI C'TryA_G
Cuts at: 826 1045 2146 2270 2386 3521 4202 4393 826
Size: 219 1101 124 116 1135 681 191 1471
Fragments arranged by size:
1471 1135 1101 681 219 191 124 116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5038
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5038
SmaI CCC'GGG
Cuts at: 1872 2365 1872
Size: 493 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3142 4043 4305 820
Size: 197 926 1199 901 262 1553
Fragments arranged by size:
1553 1199 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2829 493
Size: 2336 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5038
SphI G_CATG'C
Cuts at: 1132 2396 1132
Size: 1264 3774
SspI AAT'ATT
Cuts at: 5 52 2962 5
Size: 47 2910 2081
Fragments arranged by size:
2910 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5038
StyI C'CwwG_G
Cuts at: 513 1065 2477 513
Size: 552 1412 3074
Fragments arranged by size:
3074 1412 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3065 3082 3235 3420 3865 325
Size: 1255 1485 17 153 185 445 1498
Fragments arranged by size:
1498 1485 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 3284 4996
Size: 274 80 33 51 1443 108 1199 1712
Cuts at: 4996 96
Size: 138
Fragments arranged by size:
1712 1443 1199 274 138 108 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4998 5030 4998
Size: 32 5006
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5038
XbaI T'CTAG_A
Cuts at: 2374 2374
Size: 5038
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5038
XmaI C'CCGG_G
Cuts at: 1870 2363 1870
Size: 493 4545
XmnI GAAnn'nnTTC
Cuts at: 3167 3167
Size: 5038
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2846 276
Size: 53 83 186 936 1312 2468
Fragments arranged by size:
2468 1312 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI