Restriction map for
pFN21AE0214
(Circular) (Six-base) MAPSORT of: pFN21AE0214.seq Check: 2719 from: 1 to: 6088
pFN21AE0214 6088 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2264 3431 1937
Size: 327 1167 4594
Fragments arranged by size:
4594 1167 327
Acc65I G'GTAC_C
Cuts at: 3149 3243 3409 3149
Size: 94 166 5828
Fragments arranged by size:
5828 166 94
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3363 4151 5535 5803 1632
Size: 1731 788 1384 268 1917
Fragments arranged by size:
1917 1731 1384 788 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 2063 4919 2063
Size: 2856 3232
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 3089 1166
Size: 1923 4165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 3233 3831 5403 3233
Size: 598 1572 3918
Fragments arranged by size:
3918 1572 598
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2306 3219 3397 3623 3775 5884 1493
Size: 813 913 178 226 152 2109 1697
Fragments arranged by size:
2109 1697 913 813 226 178 152
AseI AT'TA_AT
Cuts at: 160 1032 2246 4642 160
Size: 872 1214 2396 1606
Fragments arranged by size:
2396 1606 1214 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
AvrII C'CTAG_G
Cuts at: 2941 2941
Size: 6088
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 3149 3243 3409 4864
Size: 325 217 627 1283 79 94 166 1455
Cuts at: 4864 618
Size: 1842
Fragments arranged by size:
1842 1455 1283 627 325 217 166 94
79
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 928
Size: 971 417 4700
Fragments arranged by size:
4700 971 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 2290 2473 1548
Size: 742 183 5163
Fragments arranged by size:
5163 742 183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4748 1239
Size: 3509 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2063 4521 4919 5193 5340 5751 2063
Size: 2458 398 274 147 411 2400
Fragments arranged by size:
2458 2400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2652 2682 1178
Size: 30 165 30 1249 30 4584
Fragments arranged by size:
4584 1249 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3204 1186
Size: 2018 4070
BseYI C'CCAG_C
Cuts at: 5291 5787 5291
Size: 496 5592
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2338 4149 4234 5305 2338
Size: 1811 85 1071 3121
Fragments arranged by size:
3121 1811 1071 85
BsmI GAATG_Cn'
Cuts at: 2183 2761 3599 3692 2183
Size: 578 838 93 4579
Fragments arranged by size:
4579 838 578 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2338 3407 4149 4234 5305 1391
Size: 399 548 1069 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 548 399 85
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 3443 844
Size: 1191 1408 3489
Fragments arranged by size:
3489 1408 1191
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2832 4583 4757 65
Size: 1183 1584 1751 174 1396
Fragments arranged by size:
1751 1584 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2063 4732 4919 1700
Size: 110 253 2669 187 2869
Fragments arranged by size:
2869 2669 253 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 2506 4892 2506
Size: 2386 3702
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 2877 2877
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2662 3418 3785 4170 4187 5639 5725 5737
Size: 690 756 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 756 690 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 3195 3604 4398 4418 3195
Size: 409 794 20 4865
Fragments arranged by size:
4865 794 409 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4018 1353
Size: 2665 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3363 4151 4748 5535 5803 1239
Size: 393 1731 788 597 787 268 1524
Fragments arranged by size:
1731 1524 788 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2666 2698 2766 2798 4729
Size: 32 135 32 1094 32 68 32 1931
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1931 1094 135 68 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3153 3247 3413 3153
Size: 94 166 5828
Fragments arranged by size:
5828 166 94
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 4046 4405 4564 518
Size: 648 1923 957 359 159 2042
Fragments arranged by size:
2042 1923 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3324 3504 4181 5329 5574 1606
Size: 1718 180 677 1148 245 2120
Fragments arranged by size:
2120 1718 1148 677 245 180
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 2982 387
Size: 2595 3493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2746 3446 4991 1132
Size: 97 1517 700 1545 2229
Fragments arranged by size:
2229 1545 1517 700 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2352 2445 1108
Size: 1244 93 4751
Fragments arranged by size:
4751 1244 93
PfoI T'CCnGG_A
Cuts at: 2187 2187
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3336 3368 3336
Size: 32 6056
PstI C_TGCA'G
Cuts at: 830 2229 3202 3440 830
Size: 1399 973 238 3478
Fragments arranged by size:
3478 1399 973 238
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 3324 3324
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2225 3198 3436 4571 5252 5443 826
Size: 219 1180 973 238 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 973 681 238 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 2933 152
Size: 2781 3307
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 2143 4012 5
Size: 47 2091 1869 2081
Fragments arranged by size:
2091 2081 1869 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2941 3527 513
Size: 552 1876 586 3074
Fragments arranged by size:
3074 1876 586 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4115 4132 4285 4470 4915 325
Size: 1255 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3041 4334 6046
Size: 274 80 33 51 1443 1064 1293 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
1712 1443 1293 1064 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2652 2684 6048 6080 2652
Size: 32 3364 32 2660
Fragments arranged by size:
3364 2660 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2976 4217 2976
Size: 1241 4847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI