Restriction map for
pFN21AE0212
(Circular) (Six-base) MAPSORT of: pFN21AE0212.seq Check: 5205 from: 1 to: 4888
pFN21AE0212 4888 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2165 2698 278
Size: 53 83 186 936 629 533 2468
Fragments arranged by size:
2468 936 629 533 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2231 1937
Size: 294 4594
Acc65I G'GTAC_C
Cuts at: 2209 2209
Size: 4888
AclI AA'CG_TT
Cuts at: 1563 3015 3388 1563
Size: 1452 373 3063
Fragments arranged by size:
3063 1452 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2951 4335 4603 1632
Size: 1319 1384 268 1917
Fragments arranged by size:
1917 1384 1319 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4691
AflIII A'CryG_T
Cuts at: 1203 3787 4764 1203
Size: 2584 977 1327
Fragments arranged by size:
2584 1327 977
AgeI A'CCGG_T
Cuts at: 3719 3719
Size: 4888
AhdI GACnn_n'nnGTC
Cuts at: 3617 3617
Size: 4888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4888
AlwNI CAG_nnn'CTG
Cuts at: 2181 2631 4203 2181
Size: 450 1572 2866
Fragments arranged by size:
2866 1572 450
ApaLI G'TGCA_C
Cuts at: 2945 4101 2945
Size: 1156 3732
ApoI r'AATT_y
Cuts at: 1493 2197 2423 2575 4684 1493
Size: 704 226 152 2109 1697
Fragments arranged by size:
2109 1697 704 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3442 160
Size: 872 2410 1606
Fragments arranged by size:
2410 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4888
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 1870
Size: 73 270 4545
Fragments arranged by size:
4545 270 73
BamHI G'GATC_C
Cuts at: 2218 2585 2218
Size: 367 4521
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 3664 618
Size: 325 217 627 422 1455 1842
Fragments arranged by size:
1842 1455 627 422 325 217
BanII G_rGCy'C
Cuts at: 1391 2207 1391
Size: 816 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3079 3113 1903
Size: 34 1142 34 3678
Fragments arranged by size:
3678 1142 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2780 3990 2780
Size: 1210 3678
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2121 3499 136
Size: 107 122 71 631 605 449 1378 1525
Fragments arranged by size:
1525 1378 631 605 449 122 107 71
BglII A'GATC_T
Cuts at: 4883 4883
Size: 4888
BlpI GC'TnA_GC
Cuts at: 2305 2305
Size: 4888
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2949 4105 1790
Size: 350 809 1156 2573
Fragments arranged by size:
2573 1156 809 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4888
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2619 3577 442
Size: 416 53 83 399 24 1202 958 1753
Fragments arranged by size:
1753 1202 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3548 1239
Size: 2309 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3013 3878 4176 3013
Size: 865 298 3725
Fragments arranged by size:
3725 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3551 882
Size: 617 2052 2219
Fragments arranged by size:
2219 2052 617
BsaAI yAC'GTr
Cuts at: 493 1204 2679 493
Size: 711 1475 2702
Fragments arranged by size:
2702 1475 711
BsaBI GATnn'nnATC
Cuts at: 4882 4882
Size: 4888
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2695 3077 3712
Size: 53 83 186 936 629 533 382 635
Cuts at: 3712 275
Size: 1451
Fragments arranged by size:
1451 936 635 629 533 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3321 3719 3993 4140 4551 3321
Size: 398 274 147 411 3658
Fragments arranged by size:
3658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4663
Fragments arranged by size:
4663 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4888
BseYI C'CCAG_C
Cuts at: 4091 4587 4091
Size: 496 4392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2194 1163
Size: 573 458 3857
Fragments arranged by size:
3857 573 458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2338 3099 4127 1540
Size: 275 186 337 761 1028 2301
Fragments arranged by size:
2301 1028 761 337 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 3034 4105 2949
Size: 85 1071 3732
Fragments arranged by size:
3732 1071 85
BsmI GAATG_Cn'
Cuts at: 2399 2492 2399
Size: 93 4795
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2207 2949 3034 4105 1391
Size: 399 350 67 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 85 67
BspEI T'CCGG_A
Cuts at: 4551 4551
Size: 4888
BspHI T'CATG_A
Cuts at: 2777 4507 2777
Size: 1730 3158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 844
Size: 1399 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2775 1394
Size: 429 952 3507
Fragments arranged by size:
3507 952 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3383 3557 65
Size: 1183 2135 174 1396
Fragments arranged by size:
2135 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 3532 3719 1700
Size: 110 241 1481 187 2869
Fragments arranged by size:
2869 1481 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4888
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4888
BssSI C'ACGA_G
Cuts at: 2948 3960 2948
Size: 1012 3876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2073 2088 794
Size: 1279 15 3594
Fragments arranged by size:
3594 1279 15
BstBI TT'CG_AA
Cuts at: 3692 3692
Size: 4888
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4888
BstYI r'GATC_y
Cuts at: 1972 2218 2585 2970 2987 4439 4525 4537 4883
Size: 246 367 385 17 1452 86 12 346
Cuts at: 4883 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 246 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3397
Fragments arranged by size:
3397 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4149
Fragments arranged by size:
4149 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 3198 3218 2404
Size: 794 20 4074
Fragments arranged by size:
4074 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4853
DraI TTT'AAA
Cuts at: 2192 2192
Size: 4888
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3895 809
Size: 507 2579 1802
Fragments arranged by size:
2579 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 3224 4853
Size: 54 1425 50 275 18 505 889 1629
Cuts at: 4853 8
Size: 43
Fragments arranged by size:
1629 1425 889 505 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2335 1537
Size: 275 523 4090
Fragments arranged by size:
4090 523 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2818 1353
Size: 737 728 3423
Fragments arranged by size:
3423 737 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3475 3849 3995 387
Size: 1064 138 1886 374 146 1280
Fragments arranged by size:
1886 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2951 3548 4335 4603 1239
Size: 393 1319 597 787 268 1524
Fragments arranged by size:
1524 1319 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2197 2575 2197
Size: 378 4510
FspI TGC'GCA
Cuts at: 3394 3394
Size: 4888
HaeII r_GCGC'y
Cuts at: 1678 4035 1678
Size: 2357 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3529 3561 3603 3635 1373
Size: 32 135 32 1957 32 42 32 2626
Fragments arranged by size:
2626 1957 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2232 2478 669
Size: 988 281 294 246 3079
Fragments arranged by size:
3079 988 294 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4888
HpaI GTT'AAC
Cuts at: 2478 2478
Size: 4888
KpnI G_GTAC'C
Cuts at: 2213 2213
Size: 4888
MfeI C'AATT_G
Cuts at: 2487 2487
Size: 4888
MluI A'CGCG_T
Cuts at: 4764 4764
Size: 4888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4002 4186 1305
Size: 2697 184 2007
Fragments arranged by size:
2697 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3066
Fragments arranged by size:
3066 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2846 3205 3364 518
Size: 648 1680 359 159 2042
Fragments arranged by size:
2042 1680 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2178 2304 2981 4129 4374 1606
Size: 572 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 572 245 126
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 4888
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3397
Fragments arranged by size:
3397 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4888
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 4888
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4888
NotI GC'GGCC_GC
Cuts at: 2335 2335
Size: 4888
NspI r_CATG'y
Cuts at: 1132 1229 2246 3791 1132
Size: 97 1017 1545 2229
Fragments arranged by size:
2229 1545 1017 97
PasI CC'CwG_GG
Cuts at: 1348 2140 1348
Size: 792 4096
PciI A'CATG_T
Cuts at: 3787 3787
Size: 4888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4888
PmeI GTTT'AAAC
Cuts at: 2192 2192
Size: 4888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 4470 4502 2957
Size: 32 1481 32 3343
Fragments arranged by size:
3343 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4888
PsiI TTA'TAA
Cuts at: 2458 2458
Size: 4888
PstI C_TGCA'G
Cuts at: 830 2240 830
Size: 1410 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4888
PvuII CAG'CTG
Cuts at: 2178 2178
Size: 4888
SalI G'TCGA_C
Cuts at: 1936 2230 1936
Size: 294 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 4888
SbfI CC_TGCA'GG
Cuts at: 2240 2240
Size: 4888
ScaI AGT'ACT
Cuts at: 3136 4848 3136
Size: 1712 3176
SfcI C'TryA_G
Cuts at: 826 1045 2236 3371 4052 4243 826
Size: 219 1191 1135 681 191 1471
Fragments arranged by size:
1471 1191 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4888
SmaI CCC'GGG
Cuts at: 1872 2215 1872
Size: 343 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2992 3893 4155 820
Size: 197 926 1049 901 262 1553
Fragments arranged by size:
1553 1049 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2679 493
Size: 2186 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4888
SphI G_CATG'C
Cuts at: 1132 2246 1132
Size: 1114 3774
SspI AAT'ATT
Cuts at: 5 52 2812 5
Size: 47 2760 2081
Fragments arranged by size:
2760 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4888
StyI C'CwwG_G
Cuts at: 513 1065 2004 2327 513
Size: 552 939 323 3074
Fragments arranged by size:
3074 939 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2915 2932 3085 3270 3715 325
Size: 1255 1335 17 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3134 4846 96
Size: 274 80 33 51 1443 1157 1712 138
Fragments arranged by size:
1712 1443 1157 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4848 4880 4848
Size: 32 4856
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4888
XbaI T'CTAG_A
Cuts at: 2224 2224
Size: 4888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4888
XmaI C'CCGG_G
Cuts at: 1870 2213 1870
Size: 343 4545
XmnI GAAnn'nnTTC
Cuts at: 3017 3017
Size: 4888
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2163 2696 276
Size: 53 83 186 936 629 533 2468
Fragments arranged by size:
2468 936 629 533 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI