Restriction map for
pFN21AE0209
(Circular) (Six-base) MAPSORT of: pFN21AE0209.seq Check: 3786 from: 1 to: 5614
pFN21AE0209 5614 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3424 278
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2957 1937
Size: 1020 4594
Acc65I G'GTAC_C
Cuts at: 2935 2935
Size: 5614
AclI AA'CG_TT
Cuts at: 1563 3741 4114 1563
Size: 2178 373 3063
Fragments arranged by size:
3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3677 5061 5329 1632
Size: 2045 1384 268 1917
Fragments arranged by size:
2045 1917 1384 268
AfeI AGC'GCT
Cuts at: 2302 2302
Size: 5614
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5417
AflIII A'CryG_T
Cuts at: 1203 4513 5490 1203
Size: 3310 977 1327
Fragments arranged by size:
3310 1327 977
AgeI A'CCGG_T
Cuts at: 4445 4445
Size: 5614
AhdI GACnn_n'nnGTC
Cuts at: 4343 4343
Size: 5614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5614
AlwNI CAG_nnn'CTG
Cuts at: 3357 4929 3357
Size: 1572 4042
ApaI G_GGCC'C
Cuts at: 2287 2287
Size: 5614
ApaLI G'TGCA_C
Cuts at: 3671 4827 3671
Size: 1156 4458
ApoI r'AATT_y
Cuts at: 1493 2528 2785 2923 3149 3301 5410 1493
Size: 1035 257 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 257 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 4168 160
Size: 872 3136 1606
Fragments arranged by size:
3136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
AvaI C'yCGr_G
Cuts at: 1870 1943 2939 1870
Size: 73 996 4545
Fragments arranged by size:
4545 996 73
BamHI G'GATC_C
Cuts at: 2944 3311 2944
Size: 367 5247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2858 2935 4390 618
Size: 325 217 627 1071 77 1455 1842
Fragments arranged by size:
1842 1455 1071 627 325 217 77
BanII G_rGCy'C
Cuts at: 1391 2287 2933 1391
Size: 896 646 4072
Fragments arranged by size:
4072 896 646
BbeI G_GCGC'C
Cuts at: 2862 2862
Size: 5614
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4643
BbvCI CC'TCA_GC
Cuts at: 2822 2822
Size: 5614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3805 3839 1903
Size: 34 1868 34 3678
Fragments arranged by size:
3678 1868 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3506 4716 3506
Size: 1210 4404
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 4225 136
Size: 107 122 71 631 605 354 2199 1525
Fragments arranged by size:
2199 1525 631 605 354 122 107 71
BglII A'GATC_T
Cuts at: 5609 5609
Size: 5614
BlpI GC'TnA_GC
Cuts at: 3031 3031
Size: 5614
Bme1580I G_kGCm'C
Cuts at: 1790 2287 3675 4831 1790
Size: 497 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 497
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5614
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4303 442
Size: 416 53 83 399 24 1928 958 1753
Fragments arranged by size:
1928 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2587 4274 1239
Size: 1348 1687 2579
Fragments arranged by size:
2579 1687 1348
Bpu10I CC'TnA_GC
Cuts at: 2822 2822
Size: 5614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3739 4604 4902 3739
Size: 865 298 4451
Fragments arranged by size:
4451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 4277 882
Size: 617 954 1824 2219
Fragments arranged by size:
2219 1824 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3405 493
Size: 711 2201 2702
Fragments arranged by size:
2702 2201 711
BsaBI GATnn'nnATC
Cuts at: 2321 5608 2321
Size: 3287 2327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2859 3421 3803 4438
Size: 53 83 186 936 1326 562 382 635
Cuts at: 4438 275
Size: 1451
Fragments arranged by size:
1451 1326 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2550 2681 2781 4047 4445 4719 4866 5277 2550
Size: 131 100 1266 398 274 147 411 2887
Fragments arranged by size:
2887 1266 411 398 274 147 131 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2378 2408 2570
Size: 30 165 30 855 30 90 30 162
Cuts at: 2570 2600 1178
Size: 30 4192
Fragments arranged by size:
4192 855 165 162 90 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2897 1186
Size: 1711 3903
BseYI C'CCAG_C
Cuts at: 2137 2179 2199 2280 2741 4817 5313 2137
Size: 42 20 81 461 2076 496 2438
Fragments arranged by size:
2438 2076 496 461 81 42 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 1163
Size: 573 648 4393
Fragments arranged by size:
4393 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2033 3064 3825 4853 1540
Size: 275 186 32 1031 761 1028 2301
Fragments arranged by size:
2301 1031 1028 761 275 186 32
BsiHKAI G_wGCw'C
Cuts at: 2889 3675 3760 4831 2889
Size: 786 85 1071 3672
Fragments arranged by size:
3672 1071 786 85
BsmI GAATG_Cn'
Cuts at: 3125 3218 3125
Size: 93 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2889 2933 3675 3760 4831 1391
Size: 399 497 602 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 497 399 85 44
BspEI T'CCGG_A
Cuts at: 2550 2781 5277 2550
Size: 231 2496 2887
Fragments arranged by size:
2887 2496 231
BspHI T'CATG_A
Cuts at: 2164 3503 5233 2164
Size: 1339 1730 2545
Fragments arranged by size:
2545 1730 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 2471 2969 844
Size: 1264 363 498 3489
Fragments arranged by size:
3489 1264 498 363
BsrBI CCG'CTC
Cuts at: 1394 1823 3501 1394
Size: 429 1678 3507
Fragments arranged by size:
3507 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2218 2530 4109 4283 65
Size: 1183 970 312 1579 174 1396
Fragments arranged by size:
1579 1396 1183 970 312 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 4258 4445 1700
Size: 110 296 2152 187 2869
Fragments arranged by size:
2869 2152 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 2891 96
Size: 2795 2819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5614
BssSI C'ACGA_G
Cuts at: 2045 3674 4686 2045
Size: 1629 1012 2973
Fragments arranged by size:
2973 1629 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5614
BstBI TT'CG_AA
Cuts at: 4418 4418
Size: 5614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5614
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 5614
BstYI r'GATC_y
Cuts at: 1972 2185 2944 3311 3696 3713 5165 5251 5263
Size: 213 759 367 385 17 1452 86 12
Cuts at: 5263 5609 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 759 385 367 346 213 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2801 513
Size: 552 939 797 3326
Fragments arranged by size:
3326 939 797 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4875
Fragments arranged by size:
4875 683 56
BtsI GCAGTG_nn'
Cuts at: 3130 3924 3944 3130
Size: 794 20 4800
Fragments arranged by size:
4800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5579
DraI TTT'AAA
Cuts at: 2918 2918
Size: 5614
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4621 809
Size: 507 3305 1802
Fragments arranged by size:
3305 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 3061 3950
Size: 54 1425 50 275 18 200 1031 889
Cuts at: 3950 5579 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1031 889 275 200 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2030 3061 1537
Size: 275 218 1031 4090
Fragments arranged by size:
4090 1031 275 218
EarI CTCTTCn'nnn_
Cuts at: 1353 3544 1353
Size: 2191 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 2324 4201 4575 4721 387
Size: 1064 138 486 249 1877 374 146 1280
Fragments arranged by size:
1877 1280 1064 486 374 249 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2587 3677 4274 5061 5329 1239
Size: 393 955 1090 597 787 268 1524
Fragments arranged by size:
1524 1090 955 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2902 2902
Size: 5614
EcoRI G'AATT_C
Cuts at: 2528 2785 2923 3301 2528
Size: 257 138 378 4841
Fragments arranged by size:
4841 378 257 138
FspI TGC'GCA
Cuts at: 4120 4120
Size: 5614
HaeII r_GCGC'y
Cuts at: 1678 2304 2862 4761 1678
Size: 626 558 1899 2531
Fragments arranged by size:
2531 1899 626 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 2570 2602 4255
Size: 32 135 32 587 32 379 32 1653
Cuts at: 4255 4287 4329 4361 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1653 587 379 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2958 3204 669
Size: 988 281 289 731 246 3079
Fragments arranged by size:
3079 988 731 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5614
HpaI GTT'AAC
Cuts at: 3204 3204
Size: 5614
KasI G'GCGC_C
Cuts at: 2858 2858
Size: 5614
KpnI G_GTAC'C
Cuts at: 2939 2939
Size: 5614
MfeI C'AATT_G
Cuts at: 3213 3213
Size: 5614
MluI A'CGCG_T
Cuts at: 5490 5490
Size: 5614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4728 4912 1305
Size: 3423 184 2007
Fragments arranged by size:
3423 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3792
Fragments arranged by size:
3792 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3572 3931 4090 518
Size: 648 2406 359 159 2042
Fragments arranged by size:
2406 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2183 2643 3030 3707 4855 5100 1606
Size: 531 46 460 387 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 460 387 245 46
NarI GG'CG_CC
Cuts at: 2859 2859
Size: 5614
NcoI C'CATG_G
Cuts at: 513 1065 2004 2801 513
Size: 552 939 797 3326
Fragments arranged by size:
3326 939 797 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5614
NotI GC'GGCC_GC
Cuts at: 2030 3061 2030
Size: 1031 4583
NspI r_CATG'y
Cuts at: 1132 1229 2523 2972 4517 1132
Size: 97 1294 449 1545 2229
Fragments arranged by size:
2229 1545 1294 449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5614
PciI A'CATG_T
Cuts at: 4513 4513
Size: 5614
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2199 1108
Size: 1091 4523
PmeI GTTT'AAAC
Cuts at: 2918 2918
Size: 5614
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5196 5228 3683
Size: 32 1481 32 4069
Fragments arranged by size:
4069 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2902 2902
Size: 5614
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5614
PsiI TTA'TAA
Cuts at: 3184 3184
Size: 5614
PspOMI G'GGCC_C
Cuts at: 2283 2283
Size: 5614
PstI C_TGCA'G
Cuts at: 830 2966 830
Size: 2136 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5614
PvuII CAG'CTG
Cuts at: 2643 2643
Size: 5614
SalI G'TCGA_C
Cuts at: 1936 2956 1936
Size: 1020 4594
SanDI GG'GwC_CC
Cuts at: 2902 2902
Size: 5614
SbfI CC_TGCA'GG
Cuts at: 2966 2966
Size: 5614
ScaI AGT'ACT
Cuts at: 2771 3862 5574 2771
Size: 1091 1712 2811
Fragments arranged by size:
2811 1712 1091
SfcI C'TryA_G
Cuts at: 826 1045 2962 4097 4778 4969 826
Size: 219 1917 1135 681 191 1471
Fragments arranged by size:
1917 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2860 2860
Size: 5614
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5614
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5614
SmaI CCC'GGG
Cuts at: 1872 2941 1872
Size: 1069 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3718 4619 4881 820
Size: 197 926 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3405 493
Size: 2912 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5614
SphI G_CATG'C
Cuts at: 1132 2972 1132
Size: 1840 3774
SspI AAT'ATT
Cuts at: 5 52 3538 5
Size: 47 3486 2081
Fragments arranged by size:
3486 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5614
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 2801 3053 513
Size: 552 939 477 320 252 3074
Fragments arranged by size:
3074 939 552 477 320 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2733 3641 3658 3811 3996 4441 325
Size: 1255 1153 908 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1153 908 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 2891 3860
Size: 274 80 33 51 1443 792 122 969
Cuts at: 3860 5572 96
Size: 1712 138
Fragments arranged by size:
1712 1443 969 792 274 138 122 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 5574 5606 2477
Size: 32 3065 32 2485
Fragments arranged by size:
3065 2485 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5614
XbaI T'CTAG_A
Cuts at: 2950 2950
Size: 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5614
XmaI C'CCGG_G
Cuts at: 1870 2939 1870
Size: 1069 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 3743
Size: 5614
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3422 276
Size: 53 83 186 936 1888 2468
Fragments arranged by size:
2468 1888 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI XcmI