Restriction map for
pFN21AE0208
(Circular) (Six-base) MAPSORT of: pFN21AE0208.seq Check: 6981 from: 1 to: 5818
pFN21AE0208 5818 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 3161 1937
Size: 979 245 4594
Fragments arranged by size:
4594 979 245
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2238 4717 5694 1203
Size: 1035 2479 977 1327
Fragments arranged by size:
2479 1327 1035 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 1184
Size: 1299 4519
AlwNI CAG_nnn'CTG
Cuts at: 2221 2446 3561 5133 2221
Size: 225 1115 1572 2906
Fragments arranged by size:
2906 1572 1115 225
ApaI G_GGCC'C
Cuts at: 2322 2322
Size: 5818
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2530 2945 3127 3353 3505 5614 1493
Size: 1037 415 182 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 415 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2688 3143 1870
Size: 73 309 436 455 4545
Fragments arranged by size:
4545 455 436 309 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2860 2893 2860
Size: 33 5785
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 3139 4594 618
Size: 325 217 627 620 732 1455 1842
Fragments arranged by size:
1842 1455 732 627 620 325 217
BanII G_rGCy'C
Cuts at: 1391 2322 3137 1391
Size: 931 815 4072
Fragments arranged by size:
4072 931 815
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2651 928
Size: 971 752 4095
Fragments arranged by size:
4095 971 752
BbvCI CC'TCA_GC
Cuts at: 2066 2384 2066
Size: 318 5500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 2457 4429
Size: 107 122 71 631 605 370 415 1972
Cuts at: 4429 136
Size: 1525
Fragments arranged by size:
1972 1525 631 605 415 370 122 107
71
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 2322 3879 5035 1790
Size: 532 1557 1156 2573
Fragments arranged by size:
2573 1557 1156 532
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 2341 3549
Size: 416 53 83 399 24 899 25 1208
Cuts at: 3549 4507 442
Size: 958 1753
Fragments arranged by size:
1753 1208 958 899 416 399 83 53
25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2714
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2120 2198 2354 4478 1239
Size: 869 12 78 156 2124 2579
Fragments arranged by size:
2579 2124 869 156 78 12
Bpu10I CC'TnA_GC
Cuts at: 2066 2384 2447 2066
Size: 318 63 5437
Fragments arranged by size:
5437 318 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3625 4007 4642 275
Size: 53 83 186 936 2092 382 635 1451
Fragments arranged by size:
2092 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 1178
Size: 30 165 30 607 30 4956
Fragments arranged by size:
4956 607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2129 2610 2925 1186
Size: 838 105 481 315 4079
Fragments arranged by size:
4079 838 481 315 105
BseYI C'CCAG_C
Cuts at: 2191 5021 5517 2191
Size: 2830 496 2492
Fragments arranged by size:
2830 2492 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2787 3072 1163
Size: 573 1051 285 3909
Fragments arranged by size:
3909 1051 573 285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 3268 4029 5057 1540
Size: 275 186 36 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2379 3879 3964 5035 2379
Size: 1500 85 1071 3162
Fragments arranged by size:
3162 1500 1071 85
BsmI GAATG_Cn'
Cuts at: 3102 3329 3422 3102
Size: 227 93 5498
Fragments arranged by size:
5498 227 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2075 2075
Size: 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2379 3137 3879 3964 5035 1391
Size: 399 532 57 758 742 85 1071 2174
Fragments arranged by size:
2174 1071 758 742 532 399 85 57
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 844
Size: 2329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 2232 2356 3705 1394
Size: 429 296 113 124 1349 3507
Fragments arranged by size:
3507 1349 429 296 124 113
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2307 4462 4649 1700
Size: 110 497 2155 187 2869
Fragments arranged by size:
2869 2155 497 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 2431 2433 1925
Size: 506 2 5310
Fragments arranged by size:
5310 506 2
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 2821 4622 2821
Size: 1801 4017
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2341
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2694 3148 3515 3900 3917 5369 5455 5467
Size: 722 454 367 385 17 1452 86 12
Cuts at: 5467 5813 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 722 454 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2183 513
Size: 552 939 179 4148
Fragments arranged by size:
4148 939 552 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2089 2930 4825 809
Size: 507 773 841 1895 1802
Fragments arranged by size:
1895 1802 841 773 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2295 3265
Size: 54 1425 50 275 18 204 261 970
Cuts at: 3265 4154 5783 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 970 889 275 261 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3265 1537
Size: 275 222 1231 4090
Fragments arranged by size:
4090 1231 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2712 3748 1353
Size: 1359 1036 3423
Fragments arranged by size:
3423 1359 1036
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2120 2198 2354 3881 4478 5265
Size: 393 476 12 78 156 1527 597 787
Cuts at: 5265 5533 1239
Size: 268 1524
Fragments arranged by size:
1527 1524 787 597 476 393 268 156
78 12
EcoO109I rG'GnC_Cy
Cuts at: 2338 2338
Size: 5818
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 5818
FspI TGC'GCA
Cuts at: 2285 4324 2285
Size: 2039 3779
HaeII r_GCGC'y
Cuts at: 1678 2141 2192 4965 1678
Size: 463 51 2773 2531
Fragments arranged by size:
2773 2531 463 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4459 4491 4533 4565 1373
Size: 32 135 32 2887 32 42 32 2626
Fragments arranged by size:
2887 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2244 3162 3408 669
Size: 988 281 306 918 246 3079
Fragments arranged by size:
3079 988 918 306 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 2887 3417 2887
Size: 530 5288
MluI A'CGCG_T
Cuts at: 2238 5694 2238
Size: 3456 2362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2807 4932 5116 1305
Size: 1502 2125 184 2007
Fragments arranged by size:
2125 2007 1502 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3531
Fragments arranged by size:
3531 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3004 3776 4135 4294 518
Size: 648 1838 772 359 159 2042
Fragments arranged by size:
2042 1838 772 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2199 2218 2388 3234 3911 5059 5304
Size: 418 175 19 170 846 677 1148 245
Cuts at: 5304 1606
Size: 2120
Fragments arranged by size:
2120 1148 846 677 418 245 175 170
19
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4327
Fragments arranged by size:
4327 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 2906 1108
Size: 1113 685 4020
Fragments arranged by size:
4020 1113 685
PfoI T'CCnGG_A
Cuts at: 2400 2790 2851 2400
Size: 390 61 5367
Fragments arranged by size:
5367 390 61
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2318 2318
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2442 2472 3170 830
Size: 1612 30 698 3478
Fragments arranged by size:
3478 1612 698 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2218 2218
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 2438 2468 3166 4301 4982 5173 826
Size: 219 1393 30 698 1135 681 191 1471
Fragments arranged by size:
1471 1393 1135 698 681 219 191 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2042 2042
Size: 5818
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 3145 1872
Size: 1273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2688 3922 4823 5085 820
Size: 197 926 745 1234 901 262 1553
Fragments arranged by size:
1553 1234 926 901 745 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 2827 3742 5
Size: 47 2775 915 2081
Fragments arranged by size:
2775 2081 915 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 2334 3257 513
Size: 552 939 330 923 3074
Fragments arranged by size:
3074 939 923 552 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2010 2042 5778 5810 2010
Size: 32 3736 32 2018
Fragments arranged by size:
3736 2018 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 2688 1943
Size: 745 5073
XmaI C'CCGG_G
Cuts at: 1870 3143 1870
Size: 1273 4545
XmnI GAAnn'nnTTC
Cuts at: 2827 3947 2827
Size: 1120 4698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI