Restriction map for
pFN21AE0200
(Circular) (Six-base) MAPSORT of: pFN21AE0200.seq Check: 6700 from: 1 to: 6067
pFN21AE0200 6067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 2061 4194 4567 1563
Size: 498 2133 373 3063
Fragments arranged by size:
3063 2133 498 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3315 4130 5514 5782 1632
Size: 1683 815 1384 268 1917
Fragments arranged by size:
1917 1683 1384 815 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2559 2590 2885 3810 5382 2559
Size: 31 295 925 1572 3244
Fragments arranged by size:
3244 1572 925 295 31
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 2204 3376 3602 3754 5863 1493
Size: 711 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 711 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 3392 1870
Size: 73 810 639 4545
Fragments arranged by size:
4545 810 639 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2946 3388 4843 618
Size: 325 217 627 392 767 442 1455 1842
Fragments arranged by size:
1842 1455 767 627 442 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2256 2760 3386 1391
Size: 865 504 626 4072
Fragments arranged by size:
4072 865 626 504
BbeI G_GCGC'C
Cuts at: 2183 2183
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2623 928
Size: 971 724 4372
Fragments arranged by size:
4372 971 724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 4128 5284 1790
Size: 2338 1156 2573
Fragments arranged by size:
2573 2338 1156
BmgBI CAC'GTC
Cuts at: 1318 2953 1318
Size: 1635 4432
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2880 2880
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2554 2671 2922 4727 1239
Size: 1127 188 117 251 1805 2579
Fragments arranged by size:
2579 1805 1127 251 188 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 4192 5057 5355 2924
Size: 1268 865 298 3636
Fragments arranged by size:
3636 1268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 4730 882
Size: 617 909 2322 2219
Fragments arranged by size:
2322 2219 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2180 3874 4256 4891
Size: 53 83 186 936 647 1694 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1694 1451 936 647 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2364 2394 1178
Size: 30 165 30 961 30 4851
Fragments arranged by size:
4851 961 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2147 2385 2713 2789 2822 2910 3116
Size: 958 3 238 328 76 33 88 206
Cuts at: 3116 3160 3290 1186
Size: 44 130 3963
Fragments arranged by size:
3963 958 328 238 206 130 88 76
44 33 3
BseYI C'CCAG_C
Cuts at: 2183 2365 5270 5766 2183
Size: 182 2905 496 2484
Fragments arranged by size:
2905 2484 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 1163
Size: 573 390 5104
Fragments arranged by size:
5104 573 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 2469 3517 4278 5306 1540
Size: 275 186 336 132 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 336 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2769 4128 4213 5284 2769
Size: 1359 85 1071 3552
Fragments arranged by size:
3552 1359 1071 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2488 2959 2488
Size: 471 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2760 2769 3386 4128 4213 5284
Size: 399 466 504 9 617 742 85 1071
Cuts at: 5284 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 617 504 466 399 85
9
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 3422 844
Size: 1745 833 3489
Fragments arranged by size:
3489 1745 833
BsrBI CCG'CTC
Cuts at: 1394 1823 2247 2457 3954 1394
Size: 429 424 210 1497 3507
Fragments arranged by size:
3507 1497 429 424 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 2513 1925
Size: 588 5479
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 2353 1184
Size: 1169 4898
BstYI r'GATC_y
Cuts at: 1972 2587 3189 3397 3764 4149 4166 5618 5704
Size: 615 602 208 367 385 17 1452 86
Cuts at: 5704 5716 6062 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 615 602 385 367 346 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 2545 513
Size: 552 939 327 214 4035
Fragments arranged by size:
4035 939 552 327 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 2835 1554
Size: 1281 4786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2466 2548
Size: 54 1425 50 275 18 504 132 82
Cuts at: 2548 3514 4403 6032 8
Size: 966 889 1629 43
Fragments arranged by size:
1629 1425 966 889 504 275 132 82
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2334 2466 3514 1537
Size: 275 522 132 1048 4090
Fragments arranged by size:
4090 1048 522 275 132
EarI CTCTTCn'nnn_
Cuts at: 1353 3291 3997 1353
Size: 1938 706 3423
Fragments arranged by size:
3423 1938 706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2554 2671 2922 3315 4130 4727
Size: 393 734 188 117 251 393 815 597
Cuts at: 4727 5514 5782 1239
Size: 787 268 1524
Fragments arranged by size:
1524 815 787 734 597 393 393 268
251 188 117
EcoO109I rG'GnC_Cy
Cuts at: 3087 3217 3087
Size: 130 5937
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
EcoRV GAT'ATC
Cuts at: 2194 2194
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2183 2598 2694 2984 3142 5214 1678
Size: 505 415 96 290 158 2072 2531
Fragments arranged by size:
2531 2072 505 415 290 158 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2642 2674 2873
Size: 32 135 32 512 32 526 32 199
Cuts at: 2873 2905 4708 4740 4782 4814 1373
Size: 32 1803 32 42 32 2626
Fragments arranged by size:
2626 1803 526 512 199 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2179 2179
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 3180 5181 5365 1305
Size: 1704 171 2001 184 2007
Fragments arranged by size:
2007 2001 1704 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4025 4384 4543 518
Size: 648 2859 359 159 2042
Fragments arranged by size:
2859 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 2526 2547 3483 4160 5308 5553 1606
Size: 738 182 21 936 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 738 677 245 182 21
NarI GG'CG_CC
Cuts at: 2180 2180
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PfoI T'CCnGG_A
Cuts at: 2439 3192 2439
Size: 753 5314
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3087 3087
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2172 2586 3419 830
Size: 1342 414 833 3478
Fragments arranged by size:
3478 1342 833 414
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2344 2344
Size: 6067
SacII CC_GC'GG
Cuts at: 2548 2548
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 2586 3419 2586
Size: 833 5234
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2168 2582 3415 4550 5231 5422 826
Size: 219 1123 414 833 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 833 681 414 219 191
SfoI GGC'GCC
Cuts at: 2181 2181
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2939 4171 5072 5334 820
Size: 197 926 996 1232 901 262 1553
Fragments arranged by size:
1553 1232 996 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2004 3222 3506 513
Size: 552 939 1218 284 3074
Fragments arranged by size:
3074 1218 939 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2372 4094 4111 4264 4449 4894 325
Size: 1255 792 1722 17 153 185 445 1498
Fragments arranged by size:
1722 1498 1255 792 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4313 6025 96
Size: 274 80 33 51 1443 2336 1712 138
Fragments arranged by size:
2336 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 2435 2467 3346 3378 6027 6059 2364
Size: 32 39 32 879 32 2649 32 2372
Fragments arranged by size:
2649 2372 879 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2731 3035 1316
Size: 1415 304 4348
Fragments arranged by size:
4348 1415 304
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2204 4196 2204
Size: 1992 4075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SrfI SwaI XcmI