Restriction map for
pFN21AE0196
(Circular) (Six-base) MAPSORT of: pFN21AE0196.seq Check: 6420 from: 1 to: 5953
pFN21AE0196 5953 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2646 2646
Size: 5953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2856 2901 4016 5400 5668 1632
Size: 1224 45 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1224 1115 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 2631 1166
Size: 1465 4488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2695 1184
Size: 1511 4442
AlwNI CAG_nnn'CTG
Cuts at: 2453 3696 5268 2453
Size: 1243 1572 3138
Fragments arranged by size:
3138 1572 1243
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2263 2351 3003 3262 3488 3640 5749 1493
Size: 770 88 652 259 226 152 2109 1697
Fragments arranged by size:
2109 1697 770 652 259 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3207 3278 1870
Size: 73 1264 71 4545
Fragments arranged by size:
4545 1264 73 71
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3274 4729 618
Size: 325 217 627 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3272 1391
Size: 1881 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2096 2138 2867 928
Size: 971 197 42 729 4014
Fragments arranged by size:
4014 971 729 197 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 2646 1548
Size: 1098 4855
BfrBI ATG'CAT
Cuts at: 2255 3023 3086 2255
Size: 768 63 5122
Fragments arranged by size:
5122 768 63
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2033 5948 2033
Size: 3915 2038
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2349 2600 4613 1239
Size: 948 162 251 2013 2579
Fragments arranged by size:
2579 2013 948 251 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4078 4943 5241 4078
Size: 865 298 4790
Fragments arranged by size:
4790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3760 4142 4777 275
Size: 53 83 186 936 2227 382 635 1451
Fragments arranged by size:
2227 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2718 2748 1178
Size: 30 165 30 1315 30 4383
Fragments arranged by size:
4383 1315 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5953
BseYI C'CCAG_C
Cuts at: 3162 5156 5652 3162
Size: 1994 496 3463
Fragments arranged by size:
3463 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 2846 1163
Size: 573 621 489 4270
Fragments arranged by size:
4270 621 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4014 4099 5170 4014
Size: 85 1071 4797
Fragments arranged by size:
4797 1071 85
BsmI GAATG_Cn'
Cuts at: 2449 3464 3557 2449
Size: 1015 93 4845
Fragments arranged by size:
4845 1015 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3272 4014 4099 5170 1391
Size: 399 1482 742 85 1071 2174
Fragments arranged by size:
2174 1482 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 2779 2806 3842 5572 2779
Size: 27 1036 1730 3160
Fragments arranged by size:
3160 1730 1036 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2646 3308 844
Size: 1802 662 3489
Fragments arranged by size:
3489 1802 662
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2618 3016 4448 4622 65
Size: 1183 1370 398 1432 174 1396
Fragments arranged by size:
1432 1396 1370 1183 398 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4597 4784 1700
Size: 110 2787 187 2869
Fragments arranged by size:
2869 2787 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 4042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2453 794
Size: 1659 4294
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2033 3283 3650 4035 4052 5504 5590 5602
Size: 61 1250 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1250 385 367 346 86 61
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2594 513
Size: 552 1529 3872
Fragments arranged by size:
3872 1529 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2677 3469 4263 4283 2677
Size: 792 794 20 4347
Fragments arranged by size:
4347 794 792 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2497 2532 529
Size: 35 1933 35 3950
Fragments arranged by size:
3950 1933 35 35
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2039 2591 3400
Size: 54 1425 50 275 18 209 552 809
Cuts at: 3400 4289 5918 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 809 552 275 209 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3883 1353
Size: 2530 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2349 2600 2856 2901 4016 4613
Size: 393 555 162 251 256 45 1115 597
Cuts at: 4613 5400 5668 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1115 787 597 555 393 268 256
251 162 45
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 5100 1678
Size: 3422 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3205 3237 4594 4626 4668
Size: 32 135 32 1633 32 1357 32 42
Cuts at: 4668 4700 1373
Size: 32 2626
Fragments arranged by size:
2626 1633 1357 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2719 3297 3543 669
Size: 988 281 781 578 246 3079
Fragments arranged by size:
3079 988 781 578 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3226 3552 3226
Size: 326 5627
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3130 5067 5251 1305
Size: 1825 1937 184 2007
Fragments arranged by size:
2007 1937 1825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2041 2593 10
Size: 54 1425 343 209 552 3370
Fragments arranged by size:
3370 1425 552 343 209 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2593 2631 3911 4270 4429 518
Size: 648 1427 38 1280 359 159 2042
Fragments arranged by size:
2042 1427 1280 648 359 159 38
MspA1I CmG'CkG
Cuts at: 1606 2347 3369 4046 5194 5439 1606
Size: 741 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 741 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2594 513
Size: 552 1529 3872
Fragments arranged by size:
3872 1529 552
NdeI CA'TA_TG
Cuts at: 387 3019 387
Size: 2632 3321
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NsiI A_TGCA'T
Cuts at: 2257 3025 3088 2257
Size: 768 63 5122
Fragments arranged by size:
5122 768 63
NspI r_CATG'y
Cuts at: 1132 1229 2611 3090 3311 4856 1132
Size: 97 1382 479 221 1545 2229
Fragments arranged by size:
2229 1545 1382 479 221 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PfoI T'CCnGG_A
Cuts at: 2438 2438
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspXI vC'TCGA_Gb
Cuts at: 3207 3207
Size: 5953
PstI C_TGCA'G
Cuts at: 830 2674 3305 830
Size: 1844 631 3478
Fragments arranged by size:
3478 1844 631
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2347 2347
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 2670 3301 4436 5117 5308 826
Size: 219 1625 631 1135 681 191 1471
Fragments arranged by size:
1625 1471 1135 681 631 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3207 4057 4958 5220 820
Size: 197 926 1264 850 901 262 1553
Fragments arranged by size:
1553 1264 926 901 850 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 2611 3311 1132
Size: 1479 700 3774
Fragments arranged by size:
3774 1479 700
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2594 3392 513
Size: 552 1529 798 3074
Fragments arranged by size:
3074 1529 798 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3980 3997 4150 4335 4780 325
Size: 1255 2400 17 153 185 445 1498
Fragments arranged by size:
2400 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2856 4199
Size: 274 80 33 51 1443 30 849 1343
Cuts at: 4199 5911 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1343 849 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 5913 5945 2745
Size: 32 3136 32 2753
Fragments arranged by size:
3136 2753 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 2467
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 3207 1943
Size: 1264 4689
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI