Restriction map for
pFN21AE0195
(Circular) (Six-base) MAPSORT of: pFN21AE0195.seq Check: 1260 from: 1 to: 5935
pFN21AE0195 5935 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3101 3101
Size: 5935
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3193 3278 1937
Size: 1256 85 4594
Fragments arranged by size:
4594 1256 85
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3998 5382 5650 1632
Size: 1079 1287 1384 268 1917
Fragments arranged by size:
1917 1384 1287 1079 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 3085 1166
Size: 1919 4016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2371 3678 5250 2371
Size: 1307 1572 3056
Fragments arranged by size:
3056 1572 1307
ApaLI G'TGCA_C
Cuts at: 3088 3992 5148 3088
Size: 904 1156 3875
Fragments arranged by size:
3875 1156 904
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3145 3145
Size: 5935
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2390 2471 3260 1870
Size: 73 447 81 789 4545
Fragments arranged by size:
4545 789 447 81 73
BamHI G'GATC_C
Cuts at: 3265 3632 3265
Size: 367 5568
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2160 2259 2266 3256 4711
Size: 325 217 627 373 99 7 990 1455
Cuts at: 4711 618
Size: 1842
Fragments arranged by size:
1842 1455 990 627 373 325 217 99
7
BanII G_rGCy'C
Cuts at: 1391 2036 2469 3254 1391
Size: 645 433 785 4072
Fragments arranged by size:
4072 785 645 433
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 2493 928
Size: 971 198 396 4370
Fragments arranged by size:
4370 971 396 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2254 3827 5037 2254
Size: 1573 1210 3152
Fragments arranged by size:
3152 1573 1210
BclI T'GATC_A
Cuts at: 1548 3072 1548
Size: 1524 4411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 4546 136
Size: 107 122 71 631 605 431 2443 1525
Fragments arranged by size:
2443 1525 631 605 431 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 3092 3996 5152 1790
Size: 1302 904 1156 2573
Fragments arranged by size:
2573 1302 1156 904
BmgBI CAC'GTC
Cuts at: 1318 2565 1318
Size: 1247 4688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2094 2436 3666
Size: 416 53 83 399 24 677 342 1230
Cuts at: 3666 4624 442
Size: 958 1753
Fragments arranged by size:
1753 1230 958 677 416 399 342 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2393 2645 2738 2939 2966 3169 3204
Size: 1109 45 252 93 201 27 203 35
Cuts at: 3204 4595 1239
Size: 1391 2579
Fragments arranged by size:
2579 1391 1109 252 203 201 93 45
35 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 4060 4925 5223 2219
Size: 1841 865 298 2931
Fragments arranged by size:
2931 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2697 2885 4598 882
Size: 617 1198 188 1713 2219
Fragments arranged by size:
2219 1713 1198 617 188
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 3110 5929 3110
Size: 2819 3116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 4360
Fragments arranged by size:
4360 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2747 1186
Size: 1561 4374
BseYI C'CCAG_C
Cuts at: 2036 2422 2589 5138 5634 2036
Size: 386 167 2549 496 2337
Fragments arranged by size:
2549 2337 496 386 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3079 1163
Size: 573 1343 4019
Fragments arranged by size:
4019 1343 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2961 3092 3996 4081 5152 2337
Size: 624 131 904 85 1071 3120
Fragments arranged by size:
3120 1071 904 624 131 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2337 2469 2961 3092 3254 3996
Size: 399 246 301 132 492 131 162 742
Cuts at: 3996 4081 5152 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 301 246 162
132 131 85
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2610 2712 3101 3290 844
Size: 1667 99 102 389 189 3489
Fragments arranged by size:
3489 1667 389 189 102 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 2256 2494 4579 4766 1700
Size: 110 260 186 238 2085 187 2869
Fragments arranged by size:
2869 2085 260 238 187 186 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 2305 3145 1925
Size: 380 840 4715
Fragments arranged by size:
4715 840 380
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 2713 794
Size: 1472 447 4016
Fragments arranged by size:
4016 1472 447
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 3265 3632 4017 4034 5486 5572 5584 5930
Size: 1293 367 385 17 1452 86 12 346
Cuts at: 5930 1972
Size: 1977
Fragments arranged by size:
1977 1452 1293 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 487
Size: 683 56 1079 4117
Fragments arranged by size:
4117 1079 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 2013 3239 2013
Size: 1226 4709
DraIII CAC_nnn'GTG
Cuts at: 2266 2444 3051 2266
Size: 178 607 5150
Fragments arranged by size:
5150 607 178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2495 4942 809
Size: 507 1179 2447 1802
Fragments arranged by size:
2447 1802 1179 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3382 4271 5900
Size: 54 1425 50 275 18 1552 889 1629
Cuts at: 5900 8
Size: 43
Fragments arranged by size:
1629 1552 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2829 3865 1353
Size: 1476 1036 3423
Fragments arranged by size:
3423 1476 1036
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 4522 4896 5042 387
Size: 1064 138 444 2489 374 146 1280
Fragments arranged by size:
2489 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2393 2645 2711 2738 2939 2966
Size: 393 716 45 252 66 27 201 27
Cuts at: 2966 3169 3204 3998 4595 5382 5650 1239
Size: 203 35 794 597 787 268 1524
Fragments arranged by size:
1524 794 787 716 597 393 268 252
203 201 66 45 35 27 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2729 3136 2729
Size: 407 5528
EcoO109I rG'GnC_Cy
Cuts at: 2452 2520 3124 3178 2452
Size: 68 604 54 5209
Fragments arranged by size:
5209 604 68 54
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4576 4608 4650 4682 1373
Size: 32 135 32 3004 32 42 32 2626
Fragments arranged by size:
3004 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2500 3279 3525 669
Size: 988 281 562 779 246 3079
Fragments arranged by size:
3079 988 779 562 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3085 3104 3893 4252 4411 518
Size: 648 1919 19 789 359 159 2042
Fragments arranged by size:
2042 1919 789 648 359 159 19
MspA1I CmG'CkG
Cuts at: 1606 2188 2710 2791 2954 3351 4028 5176 5421
Size: 582 522 81 163 397 677 1148 245
Cuts at: 5421 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 582 522 397 245 163
81
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 3165 3227 1108
Size: 1263 794 62 3816
Fragments arranged by size:
3816 1263 794 62
PfoI T'CCnGG_A
Cuts at: 2364 2661 2364
Size: 297 5638
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3124 3178 3124
Size: 54 5881
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PstI C_TGCA'G
Cuts at: 830 2387 2709 2784 2831 3287 830
Size: 1557 322 75 47 456 3478
Fragments arranged by size:
3478 1557 456 322 75 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2188 2710 2791 2188
Size: 522 81 5332
Fragments arranged by size:
5332 522 81
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SanDI GG'GwC_CC
Cuts at: 3178 3178
Size: 5935
SbfI CC_TGCA'GG
Cuts at: 2784 3287 2784
Size: 503 5432
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SexAI A'CCwGG_T
Cuts at: 2262 2262
Size: 5935
SfcI C'TryA_G
Cuts at: 826 1045 2383 2705 2780 2827 2927 3283 4418
Size: 219 1338 322 75 47 100 356 1135
Cuts at: 4418 5099 5290 826
Size: 681 191 1471
Fragments arranged by size:
1471 1338 1135 681 356 322 219 191
100 75 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 2103 2103
Size: 5935
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 4039 4940 5202 820
Size: 197 926 291 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 2130 2285 1517
Size: 613 155 5167
Fragments arranged by size:
5167 613 155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2868 3204 3374 513
Size: 552 939 864 336 170 3074
Fragments arranged by size:
3074 939 864 552 336 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 2954 3962 3979 4132 4317 4762
Size: 1255 875 499 1008 17 153 185 445
Cuts at: 4762 325
Size: 1498
Fragments arranged by size:
1498 1255 1008 875 499 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3198 4181 5893
Size: 274 80 33 51 1443 1221 983 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1712 1443 1221 983 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 5895 5927 2723
Size: 32 3140 32 2731
Fragments arranged by size:
3140 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SapI SfoI SrfI SwaI XcmI