Restriction map for
pFN21AE0184
(Circular) (Six-base) MAPSORT of: pFN21AE0184.seq Check: 2880 from: 1 to: 5563
pFN21AE0184 5563 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2623 2714 2864 3626 5010 5278 1632
Size: 991 91 150 762 1384 268 1917
Fragments arranged by size:
1917 1384 991 762 268 150 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 2349 4462 5439 1203
Size: 1146 2113 977 1327
Fragments arranged by size:
2113 1327 1146 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 4648
AlwNI CAG_nnn'CTG
Cuts at: 2509 2526 3306 4878 2509
Size: 17 780 1572 3194
Fragments arranged by size:
3194 1572 780 17
ApaLI G'TGCA_C
Cuts at: 2702 3620 4776 2702
Size: 918 1156 3489
Fragments arranged by size:
3489 1156 918
ApoI r'AATT_y
Cuts at: 1493 2277 2450 2459 2872 3098 3250 5359 1493
Size: 784 173 9 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 784 413 226 173 152 9
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 2888 1870
Size: 73 181 764 4545
Fragments arranged by size:
4545 764 181 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2884 4339 618
Size: 325 217 627 1097 1455 1842
Fragments arranged by size:
1842 1455 1097 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2626 2882 1391
Size: 1235 256 4072
Fragments arranged by size:
4072 1235 256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 2748 2852 928
Size: 971 237 612 104 3639
Fragments arranged by size:
3639 971 612 237 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4174 136
Size: 107 122 71 631 605 337 2165 1525
Fragments arranged by size:
2165 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2120 5558 2120
Size: 3438 2125
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 2706 3624 4780 1790
Size: 916 918 1156 2573
Fragments arranged by size:
2573 1156 918 916
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 3294 4252
Size: 416 53 83 399 24 1212 665 958
Cuts at: 4252 442
Size: 1753
Fragments arranged by size:
1753 1212 958 665 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2407 2476 2843 4223 1239
Size: 1168 69 367 1380 2579
Fragments arranged by size:
2579 1380 1168 367 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2352 3354 493
Size: 711 1148 1002 2702
Fragments arranged by size:
2702 1148 1002 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3370 3752 4387 275
Size: 53 83 186 936 1837 382 635 1451
Fragments arranged by size:
1837 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2094 2157 2251 2549 3996 4394 4668 4815 5226
Size: 63 94 298 1447 398 274 147 411
Cuts at: 5226 2094
Size: 2431
Fragments arranged by size:
2431 1447 411 398 298 274 147 94
63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 2775 4766 5262 2775
Size: 1991 496 3076
Fragments arranged by size:
3076 1991 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4990
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2706 3624 3709 4780 2706
Size: 918 85 1071 3489
Fragments arranged by size:
3489 1071 918 85
BsmI GAATG_Cn'
Cuts at: 2261 2758 3074 3167 2261
Size: 497 316 93 4657
Fragments arranged by size:
4657 497 316 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2626 2706 2882 3624 3709 4780 1391
Size: 399 836 80 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 836 742 399 176 85 80
BspEI T'CCGG_A
Cuts at: 2094 2157 2549 5226 2094
Size: 63 392 2677 2431
Fragments arranged by size:
2677 2431 392 63
BspHI T'CATG_A
Cuts at: 2424 2832 3452 5182 2424
Size: 408 620 1730 2805
Fragments arranged by size:
2805 1730 620 408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 844
Size: 2074 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 3450 1394
Size: 429 213 1414 3507
Fragments arranged by size:
3507 1414 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 4058 4232 65
Size: 1183 2810 174 1396
Fragments arranged by size:
2810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 4207 4394 1700
Size: 110 235 2162 187 2869
Fragments arranged by size:
2869 2162 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 2694 96
Size: 2598 2965
BssHII G'CGCG_C
Cuts at: 1925 2023 1925
Size: 98 5465
BssSI C'ACGA_G
Cuts at: 2625 3623 4635 2625
Size: 998 1012 3553
Fragments arranged by size:
3553 1012 998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5563
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2120 2546 2725 2893 3260 3645 3662 5114
Size: 148 426 179 168 367 385 17 1452
Cuts at: 5114 5200 5212 5558 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 426 385 367 346 179 168
148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2029 513
Size: 552 939 25 4047
Fragments arranged by size:
4047 939 552 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4824
Fragments arranged by size:
4824 683 56
BtsI GCAGTG_nn'
Cuts at: 3079 3873 3893 3079
Size: 794 20 4749
Fragments arranged by size:
4749 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2375 4570 809
Size: 507 1059 2195 1802
Fragments arranged by size:
2195 1802 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3010 3899 5528
Size: 54 1425 50 275 18 1180 889 1629
Cuts at: 5528 8
Size: 43
Fragments arranged by size:
1629 1425 1180 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3493 1353
Size: 2140 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4150 4524 4670 387
Size: 1064 138 2561 374 146 1280
Fragments arranged by size:
2561 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2407 2476 2623 2714 2843 2864 3626
Size: 393 775 69 147 91 129 21 762
Cuts at: 3626 4223 5010 5278 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 775 762 597 393 268 147
129 91 69 21
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 4710 1678
Size: 3032 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 4204 4236 4278
Size: 32 135 32 599 32 2001 32 42
Cuts at: 4278 4310 1373
Size: 32 2626
Fragments arranged by size:
2626 2001 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2493 2611 2644 4677 4861 1305
Size: 1188 118 33 2033 184 2007
Fragments arranged by size:
2033 2007 1188 184 118 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3741
Fragments arranged by size:
3741 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 3521 3880 4039 518
Size: 648 1603 752 359 159 2042
Fragments arranged by size:
2042 1603 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2062 2136 2692 2979 3656 4804 5049
Size: 425 31 74 556 287 677 1148 245
Cuts at: 5049 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 556 425 287 245 74
31
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 5563
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4072
Fragments arranged by size:
4072 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2485 2921 4466 1132
Size: 97 1256 436 1545 2229
Fragments arranged by size:
2229 1545 1256 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PfoI T'CCnGG_A
Cuts at: 2492 2492
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PmlI CAC'GTG
Cuts at: 2352 2352
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2061 2078 2915 830
Size: 1231 17 837 3478
Fragments arranged by size:
3478 1231 837 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
PvuII CAG'CTG
Cuts at: 2136 2692 2136
Size: 556 5007
SacII CC_GC'GG
Cuts at: 2032 2032
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2057 2074 2911 4046 4727 4918 826
Size: 219 1012 17 837 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 837 681 219 191 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2124 3667 4568 4830 820
Size: 197 926 181 1543 901 262 1553
Fragments arranged by size:
1553 1543 926 901 262 197 181
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2004 3002 513
Size: 552 939 998 3074
Fragments arranged by size:
3074 998 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3590 3607 3760 3945 4390 325
Size: 1255 2010 17 153 185 445 1498
Fragments arranged by size:
2010 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2327 2694 3809
Size: 274 80 33 51 1443 350 367 1115
Cuts at: 3809 5521 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1115 367 350 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5523 5555 5523
Size: 32 5531
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 2124 1943
Size: 181 5382
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 3692 3692
Size: 5563
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI