Restriction map for
pFN21AE0183
(Circular) (Six-base) MAPSORT of: pFN21AE0183.seq Check: 8403 from: 1 to: 5671
pFN21AE0183 5671 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3734 5118 5386 1632
Size: 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2947 4570 5547 1203
Size: 1744 1623 977 1327
Fragments arranged by size:
1744 1623 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2848 1166
Size: 1682 3989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2526 2558 2526
Size: 32 5639
AlwNI CAG_nnn'CTG
Cuts at: 2720 3414 4986 2720
Size: 694 1572 3405
Fragments arranged by size:
3405 1572 694
ApaLI G'TGCA_C
Cuts at: 2796 3728 4884 2796
Size: 932 1156 3583
Fragments arranged by size:
3583 1156 932
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2577 2588 4225 160
Size: 872 1545 11 1637 1606
Fragments arranged by size:
1637 1606 1545 872 11
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2996 1870
Size: 73 192 861 4545
Fragments arranged by size:
4545 861 192 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 2992 4447 618
Size: 325 217 627 687 518 1455 1842
Fragments arranged by size:
1842 1455 687 627 518 325 217
BanII G_rGCy'C
Cuts at: 1391 2990 1391
Size: 1599 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BfrBI ATG'CAT
Cuts at: 2504 2504
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2800 2860 3732 4888 1790
Size: 1010 60 872 1156 2573
Fragments arranged by size:
2573 1156 1010 872 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 4331 1239
Size: 792 2300 2579
Fragments arranged by size:
2579 2300 792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2891 2921 1178
Size: 30 165 30 1488 30 3928
Fragments arranged by size:
3928 1488 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2800 3732 3817 4888 2800
Size: 932 85 1071 3583
Fragments arranged by size:
3583 1071 932 85
BsmI GAATG_Cn'
Cuts at: 2616 3182 3275 2616
Size: 566 93 5012
Fragments arranged by size:
5012 566 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2412 2412
Size: 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2800 2860 2990 3732 3817 4888 1391
Size: 399 1010 60 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 1010 742 399 130 85 60
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2347 4315 4502 1700
Size: 110 537 1968 187 2869
Fragments arranged by size:
2869 1968 537 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2919 3001 3368 3753 3770 5222 5308 5320
Size: 947 82 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 947 385 367 346 86 82
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2786 513
Size: 552 1721 3398
Fragments arranged by size:
3398 1721 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 3187 3981 4001 2372
Size: 815 794 20 4042
Fragments arranged by size:
4042 815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2395 2430 529
Size: 35 1831 35 3770
Fragments arranged by size:
3770 1831 35 35
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2727 3118 4007
Size: 54 1425 50 275 18 897 391 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 897 889 391 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2771 3601 1353
Size: 1418 830 3423
Fragments arranged by size:
3423 1418 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 3734 4331 5118 5386 1239
Size: 393 399 1703 597 787 268 1524
Fragments arranged by size:
1703 1524 787 597 399 393 268
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2121 3015 3261 669
Size: 988 281 183 894 246 3079
Fragments arranged by size:
3079 988 894 281 246 183
HindIII A'AGCT_T
Cuts at: 748 2893 748
Size: 2145 3526
HpaI GTT'AAC
Cuts at: 2121 3261 2121
Size: 1140 4531
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 2947 5547 2947
Size: 2600 3071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2729 10
Size: 54 1425 343 897 2952
Fragments arranged by size:
2952 1425 897 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2848 2885 3629 3988 4147 518
Size: 648 1137 545 37 744 359 159 2042
Fragments arranged by size:
2042 1137 744 648 545 359 159 37
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NaeI GCC'GGC
Cuts at: 2349 2349
Size: 5671
NcoI C'CATG_G
Cuts at: 513 1065 2786 513
Size: 552 1721 3398
Fragments arranged by size:
3398 1721 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2347 2347
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NsiI A_TGCA'T
Cuts at: 2506 2506
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2519 3029 4574 1132
Size: 97 1290 510 1545 2229
Fragments arranged by size:
2229 1545 1290 510 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PfoI T'CCnGG_A
Cuts at: 2699 2699
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 3019 4154 4835 5026 826
Size: 219 1974 1135 681 191 1471
Fragments arranged by size:
1974 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3775 4676 4938 820
Size: 197 926 192 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 2581 3595 5
Size: 47 2529 1014 2081
Fragments arranged by size:
2529 2081 1014 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2786 3110 513
Size: 552 1721 324 3074
Fragments arranged by size:
3074 1721 552 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2815 3698 3715 3868 4053 4498 325
Size: 1255 1235 883 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1235 883 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 2220 2952
Size: 274 80 33 51 1443 178 65 732
Cuts at: 2952 3917 5629 96
Size: 965 1712 138
Fragments arranged by size:
1712 1443 965 732 274 178 138 80
65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2707
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 2135 1943
Size: 192 5479
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2780 3800 2780
Size: 1020 4651
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI