Restriction map for
pFN21AE0181
(Circular) (Six-base) MAPSORT of: pFN21AE0181.seq Check: 8501 from: 1 to: 5512
pFN21AE0181 5512 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 2855 1937
Size: 748 170 4594
Fragments arranged by size:
4594 748 170
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 3575 4959 5227 1632
Size: 801 1142 1384 268 1917
Fragments arranged by size:
1917 1384 1142 801 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 4411 5388 1203
Size: 3208 977 1327
Fragments arranged by size:
3208 1327 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2337 2510 1184
Size: 1153 173 4186
Fragments arranged by size:
4186 1153 173
AlwNI CAG_nnn'CTG
Cuts at: 2077 3255 4827 2077
Size: 1178 1572 2762
Fragments arranged by size:
2762 1572 1178
ApaI G_GGCC'C
Cuts at: 2774 2774
Size: 5512
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AscI GG'CGCG_CC
Cuts at: 2090 2090
Size: 5512
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2842 3209 2842
Size: 367 5145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2833 4288 618
Size: 325 217 627 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2295 2459 2774 2831 1391
Size: 904 164 315 57 4072
Fragments arranged by size:
4072 904 315 164 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 928
Size: 971 307 4234
Fragments arranged by size:
4234 971 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2251 2285 3703 3737 1903
Size: 34 314 34 1418 34 3678
Fragments arranged by size:
3678 1418 314 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 2103 1548
Size: 555 4957
BfrBI ATG'CAT
Cuts at: 2698 2698
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4123 136
Size: 107 122 71 631 605 2451 1525
Fragments arranged by size:
2451 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2570 2774 3573 4729 1790
Size: 240 540 204 799 1156 2573
Fragments arranged by size:
2573 1156 799 540 240 204
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 4201 442
Size: 416 53 83 399 24 1826 958 1753
Fragments arranged by size:
1826 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 4172 1239
Size: 899 2034 2579
Fragments arranged by size:
2579 2034 899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4175 882
Size: 617 2676 2219
Fragments arranged by size:
2676 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 3303 493
Size: 711 882 1217 2702
Fragments arranged by size:
2702 1217 882 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2436 2532 3319 3701
Size: 53 83 186 936 903 96 787 382
Cuts at: 3701 4336 275
Size: 635 1451
Fragments arranged by size:
1451 936 903 787 635 382 186 96
83 53
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 1178
Size: 30 165 30 1072 30 4185
Fragments arranged by size:
4185 1072 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 1186
Size: 1205 4307
BseYI C'CCAG_C
Cuts at: 2227 4715 5211 2227
Size: 2488 496 2528
Fragments arranged by size:
2528 2488 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 1163
Size: 573 558 4381
Fragments arranged by size:
4381 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2962 3723 4751 1540
Size: 275 186 33 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2459 3573 3658 4729 2459
Size: 1114 85 1071 3242
Fragments arranged by size:
3242 1114 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2295 2459 2570 2774 2831 3573
Size: 399 240 265 164 111 204 57 742
Cuts at: 3573 3658 4729 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 265 240 204 164
111 85 57
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 2511 3401 5131 2511
Size: 890 1730 2892
Fragments arranged by size:
2892 1730 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2202 2867 844
Size: 1241 117 665 3489
Fragments arranged by size:
3489 1241 665 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4007 4181 65
Size: 1183 2759 174 1396
Fragments arranged by size:
2759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4156 4343 1700
Size: 110 2346 187 2869
Fragments arranged by size:
2869 2346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 2090 1925
Size: 165 5347
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 4229
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507
Size: 870 367 385 17 1452 86 12 346
Cuts at: 5507 1972
Size: 1977
Fragments arranged by size:
1977 1452 870 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2787 513
Size: 552 939 783 3238
Fragments arranged by size:
3238 939 783 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4773
Fragments arranged by size:
4773 683 56
BtsI GCAGTG_nn'
Cuts at: 3028 3822 3842 3028
Size: 794 20 4698
Fragments arranged by size:
4698 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2602 2816 2602
Size: 214 5298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2589 2959
Size: 54 1425 50 275 18 201 558 370
Cuts at: 2959 3848 5477 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 558 370 275 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 2959 1537
Size: 275 219 928 4090
Fragments arranged by size:
4090 928 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 3442 1353
Size: 1061 1028 3423
Fragments arranged by size:
3423 1061 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2457 2457
Size: 5512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2433 3575 4172 4959 5227 1239
Size: 393 506 295 1142 597 787 268 1524
Fragments arranged by size:
1524 1142 787 597 506 393 295 268
EcoO109I rG'GnC_Cy
Cuts at: 2771 2771
Size: 5512
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 4018 4018
Size: 5512
HaeII r_GCGC'y
Cuts at: 1678 4659 1678
Size: 2981 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 4153 4185 4227
Size: 32 135 32 903 32 1646 32 42
Cuts at: 4227 4259 1373
Size: 32 2626
Fragments arranged by size:
2626 1646 903 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 2931
Fragments arranged by size:
2931 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2735 3470 3829 3988 518
Size: 648 966 603 735 359 159 2042
Fragments arranged by size:
2042 966 735 648 603 359 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2928 3605 4753 4998 1606
Size: 630 692 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 630 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2787 513
Size: 552 939 783 3238
Fragments arranged by size:
3238 939 783 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NsiI A_TGCA'T
Cuts at: 2700 2700
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2428 2476 2870 4415 1132
Size: 97 1199 48 394 1545 2229
Fragments arranged by size:
2229 1545 1199 394 97 48
PasI CC'CwG_GG
Cuts at: 1348 2371 2767 2774 1348
Size: 1023 396 7 4086
Fragments arranged by size:
4086 1023 396 7
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 5512
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PmlI CAC'GTG
Cuts at: 2086 2086
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PspOMI G'GGCC_C
Cuts at: 2770 2770
Size: 5512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2174 2206 2174
Size: 32 5480
PstI C_TGCA'G
Cuts at: 830 2316 2864 830
Size: 1486 548 3478
Fragments arranged by size:
3478 1486 548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
PvuII CAG'CTG
Cuts at: 2236 2236
Size: 5512
SacI G_AGCT'C
Cuts at: 2459 2459
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SapI GCTCTTCn'nnn_
Cuts at: 2414 2414
Size: 5512
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 3760 5472 3760
Size: 1712 3800
SfcI C'TryA_G
Cuts at: 826 1045 2312 2326 2860 3995 4676 4867 826
Size: 219 1267 14 534 1135 681 191 1471
Fragments arranged by size:
1471 1267 1135 681 534 219 191 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 2382 1517
Size: 865 4647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2787 2951 513
Size: 552 939 783 164 3074
Fragments arranged by size:
3074 939 783 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3556 3709 3894 4339 325
Size: 1255 1959 17 153 185 445 1498
Fragments arranged by size:
1959 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 3758 5470
Size: 274 80 33 51 1443 492 1289 1712
Cuts at: 5470 96
Size: 138
Fragments arranged by size:
1712 1443 1289 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 2731 1316
Size: 1415 4097
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PpuMI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI