Restriction map for
pFN21AE0179
(Circular) (Six-base) MAPSORT of: pFN21AE0179.seq Check: 7231 from: 1 to: 5731
pFN21AE0179 5731 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 3074 1937
Size: 664 473 4594
Fragments arranged by size:
4594 664 473
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2872 3474 5046 2872
Size: 602 1572 3557
Fragments arranged by size:
3557 1572 602
ApaLI G'TGCA_C
Cuts at: 2160 3788 4944 2160
Size: 1628 1156 2947
Fragments arranged by size:
2947 1628 1156
ApoI r'AATT_y
Cuts at: 1493 2961 3040 3266 3418 5527 1493
Size: 1468 79 226 152 2109 1697
Fragments arranged by size:
2109 1697 1468 226 152 79
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2299 3056 1870
Size: 73 86 270 757 4545
Fragments arranged by size:
4545 757 270 86 73
AvrII C'CTAG_G
Cuts at: 2998 2998
Size: 5731
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2093 2115 2271 2524 2729 3050 1391
Size: 702 22 156 253 205 321 4072
Fragments arranged by size:
4072 702 321 253 205 156 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 928
Size: 971 764 3996
Fragments arranged by size:
3996 971 764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2290 2324 2392 2426 3922 3956 1903
Size: 34 353 34 68 34 1496 34 3678
Fragments arranged by size:
3678 1496 353 68 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 2638 1548
Size: 1090 4641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2097 2311 4342
Size: 107 122 71 631 605 425 214 2031
Cuts at: 4342 136
Size: 1525
Fragments arranged by size:
2031 1525 631 605 425 214 122 107
71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 2844 3148 2844
Size: 304 5427
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2164 2260 3792 4948 1790
Size: 287 87 96 1532 1156 2573
Fragments arranged by size:
2573 1532 1156 287 96 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 2727 3462
Size: 416 53 83 399 24 987 323 735
Cuts at: 3462 4420 442
Size: 958 1753
Fragments arranged by size:
1753 987 958 735 416 399 323 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2103 2633 4391 1239
Size: 850 14 530 1758 2579
Fragments arranged by size:
2579 1758 850 530 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2142 4164 4562 4836 4983 5394 2142
Size: 2022 398 274 147 411 2479
Fragments arranged by size:
2479 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2938 2968 1178
Size: 30 165 30 1535 30 3941
Fragments arranged by size:
3941 1535 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2757 1186
Size: 861 710 4160
Fragments arranged by size:
4160 861 710
BseYI C'CCAG_C
Cuts at: 2100 2545 2916 4934 5430 2100
Size: 445 371 2018 496 2401
Fragments arranged by size:
2401 2018 496 445 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 1163
Size: 573 909 4249
Fragments arranged by size:
4249 909 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2164 3792 3877 4948 2115
Size: 49 1628 85 1071 2898
Fragments arranged by size:
2898 1628 1071 85 49
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2093 2115 2164 2260 2271 2524
Size: 399 287 16 22 49 96 11 253
Cuts at: 2524 2729 3050 3792 3877 4948 1391
Size: 205 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 321 287 253 205
96 85 49 22 16 11
BspEI T'CCGG_A
Cuts at: 2142 5394 2142
Size: 3252 2479
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2045 2937 4226 4400 65
Size: 1183 797 892 1289 174 1396
Fragments arranged by size:
1396 1289 1183 892 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 2411 3791 4803 2411
Size: 1380 1012 3339
Fragments arranged by size:
3339 1380 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3009 794
Size: 2215 3516
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2399 2399
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2498 2838 2925 2498
Size: 340 87 5304
Fragments arranged by size:
5304 340 87
BtgI C'CryG_G
Cuts at: 513 1065 2004 2424 513
Size: 552 939 420 3820
Fragments arranged by size:
3820 939 552 420
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 2883 3247 4041 4061 2883
Size: 364 794 20 4553
Fragments arranged by size:
4553 794 364 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2539 3009 2539
Size: 470 5261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2421 3178
Size: 54 1425 50 275 18 292 299 757
Cuts at: 3178 4067 5696 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 757 299 292 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 3661 1353
Size: 834 1474 3423
Fragments arranged by size:
3423 1474 834
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2113 2113
Size: 5731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2103 2633 3794 4391 5178 5446
Size: 393 457 14 530 1161 597 787 268
Cuts at: 5446 1239
Size: 1524
Fragments arranged by size:
1524 1161 787 597 530 457 393 268
14
EcoO109I rG'GnC_Cy
Cuts at: 2128 2221 2744 2128
Size: 93 523 5115
Fragments arranged by size:
5115 523 93
EcoRI G'AATT_C
Cuts at: 2961 3040 3418 2961
Size: 79 378 5274
Fragments arranged by size:
5274 378 79
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 2428 2460 4372
Size: 32 135 32 702 32 122 32 1912
Cuts at: 4372 4404 4446 4478 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1912 702 135 122 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 10
Size: 54 1425 343 292 3617
Fragments arranged by size:
3617 1425 343 292 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2426 2510 2738 2869 3147 3824 4972 5217
Size: 820 84 228 131 278 677 1148 245
Cuts at: 5217 1606
Size: 2120
Fragments arranged by size:
2120 1148 820 677 278 245 228 131
84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2407 3089 4634 1132
Size: 97 1178 682 1545 2229
Fragments arranged by size:
2229 1545 1178 682 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2872 1108
Size: 1764 3967
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2221 2128
Size: 93 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspXI vC'TCGA_Gb
Cuts at: 2299 2299
Size: 5731
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2510 2869 2510
Size: 359 5372
SacI G_AGCT'C
Cuts at: 2115 2115
Size: 5731
SacII CC_GC'GG
Cuts at: 2427 2427
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SapI GCTCTTCn'nnn_
Cuts at: 2187 2187
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 3079 4214 4895 5086 826
Size: 219 2034 1135 681 191 1471
Fragments arranged by size:
2034 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2031 3058 1872
Size: 159 1027 4545
Fragments arranged by size:
4545 1027 159
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 3835 4736 4998 820
Size: 197 926 356 1536 901 262 1553
Fragments arranged by size:
1553 1536 926 901 356 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 2407 3089 1132
Size: 1275 682 3774
Fragments arranged by size:
3774 1275 682
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 2305 1517
Size: 788 4943
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 2998 3170 513
Size: 552 939 378 616 172 3074
Fragments arranged by size:
3074 939 616 552 378 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 3758 3775 3928 4113 4558 325
Size: 1255 599 1579 17 153 185 445 1498
Fragments arranged by size:
1579 1498 1255 599 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3977 5689
Size: 274 80 33 51 1443 258 1742 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1742 1712 1443 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2938 2970 5691 5723 2938
Size: 32 2721 32 2946
Fragments arranged by size:
2946 2721 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 2299 1943
Size: 356 5375
XmaI C'CCGG_G
Cuts at: 1870 2029 3056 1870
Size: 159 1027 4545
Fragments arranged by size:
4545 1027 159
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI