Restriction map for
pFN21AE0176
(Circular) (Six-base) MAPSORT of: pFN21AE0176.seq Check: 3941 from: 1 to: 7036
pFN21AE0176 7036 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3171 3697 4071 4310 4379 1937
Size: 1234 526 374 239 69 4594
Fragments arranged by size:
4594 1234 526 374 239 69
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 5163 5536 1563
Size: 3600 373 3063
Fragments arranged by size:
3600 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 3071 3108 5099 6483 6751 1632
Size: 388 1051 37 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 1051 388 268 37
AflII C'TTAA_G
Cuts at: 820 1017 3149 820
Size: 197 2132 4707
Fragments arranged by size:
4707 2132 197
AflIII A'CryG_T
Cuts at: 1203 2312 3946 5935 6912 1203
Size: 1109 1634 1989 977 1327
Fragments arranged by size:
1989 1634 1327 1109 977
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 2854 1166
Size: 1688 5348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4106 1184
Size: 2922 4114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 2713 2745 2286
Size: 32 395 32 6577
Fragments arranged by size:
6577 395 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2383 3304 4779 6351 2383
Size: 921 1475 1572 3068
Fragments arranged by size:
3068 1572 1475 921
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 2264 2387 2586 3554 3602 3820 4280 4345
Size: 771 123 199 968 48 218 460 65
Cuts at: 4345 4571 4723 6832 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 771 460 226 218 199
152 123 65 48
AseI AT'TA_AT
Cuts at: 160 1032 2580 5590 160
Size: 872 1548 3010 1606
Fragments arranged by size:
3010 1606 1548 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 4001 4189 4361 1870
Size: 73 2058 188 172 4545
Fragments arranged by size:
4545 2058 188 172 73
AvrII C'CTAG_G
Cuts at: 2365 2365
Size: 7036
BamHI G'GATC_C
Cuts at: 3268 4366 4733 3268
Size: 1098 367 5571
Fragments arranged by size:
5571 1098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3506 4357 5812 618
Size: 325 217 627 1719 851 1455 1842
Fragments arranged by size:
1842 1719 1455 851 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4355 1391
Size: 2964 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4338 928
Size: 971 2439 3626
Fragments arranged by size:
3626 2439 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5227 5261 1903
Size: 34 3290 34 3678
Fragments arranged by size:
3678 3290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4928 6138 4928
Size: 1210 5826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7036
BfrBI ATG'CAT
Cuts at: 2166 2166
Size: 7036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4023 5647 136
Size: 107 122 71 631 605 2351 1624 1525
Fragments arranged by size:
2351 1624 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2806 2814 7031 2806
Size: 8 4217 2811
Fragments arranged by size:
4217 2811 8
BlpI GC'TnA_GC
Cuts at: 3855 4453 3855
Size: 598 6438
Bme1580I G_kGCm'C
Cuts at: 1790 5097 6253 1790
Size: 3307 1156 2573
Fragments arranged by size:
3307 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2911 1318
Size: 1593 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4767 5725 442
Size: 416 53 83 399 24 3350 958 1753
Fragments arranged by size:
3350 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7036
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4225 4225
Size: 7036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2504 2881 3614 4136 5696 1239
Size: 887 378 377 733 522 1560 2579
Fragments arranged by size:
2579 1560 887 733 522 378 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 3250 5161 6026 6324 2000
Size: 1250 1911 865 298 2712
Fragments arranged by size:
2712 1911 1250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3630 5699 882
Size: 617 2131 2069 2219
Fragments arranged by size:
2219 2131 2069 617
BsaAI yAC'GTr
Cuts at: 493 1204 3947 4827 493
Size: 711 2743 880 2702
Fragments arranged by size:
2743 2702 880 711
BsaBI GATnn'nnATC
Cuts at: 7030 7030
Size: 7036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4843 5225 5860 275
Size: 53 83 186 936 3310 382 635 1451
Fragments arranged by size:
3310 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4198 5469 5867 6141 6288 6699 4198
Size: 1271 398 274 147 411 4535
Fragments arranged by size:
4535 1271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2118 2148 2713 2743 4034
Size: 30 165 30 715 30 565 30 1291
Cuts at: 4034 4064 1178
Size: 30 4150
Fragments arranged by size:
4150 1291 715 565 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2619 2622 2625 2628 2631 2634 3227 4201
Size: 1433 3 3 3 3 3 593 974
Cuts at: 4201 4204 1186
Size: 3 4018
Fragments arranged by size:
4018 1433 974 593 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2505 3993 4135 6239 6735 2505
Size: 1488 142 2104 496 2806
Fragments arranged by size:
2806 2104 1488 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3911 4267 1163
Size: 573 2175 356 3932
Fragments arranged by size:
3932 2175 573 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4486 5247 6275 1540
Size: 275 186 2485 761 1028 2301
Fragments arranged by size:
2485 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2884 3689 5097 5182 6253 2884
Size: 805 1408 85 1071 3667
Fragments arranged by size:
3667 1408 1071 805 85
BsmI GAATG_Cn'
Cuts at: 3143 3154 3768 4547 4640 3143
Size: 11 614 779 93 5539
Fragments arranged by size:
5539 779 614 93 11
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2884 3689 4355 5097 5182 6253 1391
Size: 399 1094 805 666 742 85 1071 2174
Fragments arranged by size:
2174 1094 1071 805 742 666 399 85
BspEI T'CCGG_A
Cuts at: 6699 6699
Size: 7036
BspHI T'CATG_A
Cuts at: 4925 6655 4925
Size: 1730 5306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3933 4391 844
Size: 3089 458 3489
Fragments arranged by size:
3489 3089 458
BsrBI CCG'CTC
Cuts at: 1394 1823 4923 1394
Size: 429 3100 3507
Fragments arranged by size:
3507 3100 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2166 5531 5705 65
Size: 1183 918 3365 174 1396
Fragments arranged by size:
3365 1396 1183 918 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5680 5867 1700
Size: 110 3870 187 2869
Fragments arranged by size:
3870 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7036
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7036
BssSI C'ACGA_G
Cuts at: 5096 6108 5096
Size: 1012 6024
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7036
BstBI TT'CG_AA
Cuts at: 5840 5840
Size: 7036
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7036
BstYI r'GATC_y
Cuts at: 1972 2806 2814 3268 3492 3847 4366 4733 5118
Size: 834 8 454 224 355 519 367 385
Cuts at: 5118 5135 6587 6673 6685 7031 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 834 519 454 385 367 355
346 224 86 17 12 8
BstZ17I GTA'TAC
Cuts at: 3172 3698 3172
Size: 526 6510
Bsu36I CC'TnA_GG
Cuts at: 2593 2593
Size: 7036
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6297
Fragments arranged by size:
6297 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3477 4111 4552 5346 5366 2884
Size: 593 634 441 794 20 4554
Fragments arranged by size:
4554 794 634 593 441 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7036
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 3724 4340 3724
Size: 616 6420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6043 809
Size: 507 4727 1802
Fragments arranged by size:
4727 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4483 5372 7001
Size: 54 1425 50 275 18 2653 889 1629
Cuts at: 7001 8
Size: 43
Fragments arranged by size:
2653 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4483 1537
Size: 275 2671 4090
Fragments arranged by size:
4090 2671 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3084 3217 4966 1353
Size: 1731 133 1749 3423
Fragments arranged by size:
3423 1749 1731 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5623 5997 6143 387
Size: 1064 138 4034 374 146 1280
Fragments arranged by size:
4034 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2126 2504 2881 3071 3108 3614
Size: 393 388 106 378 377 190 37 506
Cuts at: 3614 4136 5099 5696 6483 6751 1239
Size: 522 963 597 787 268 1524
Fragments arranged by size:
1524 963 787 597 522 506 393 388
378 377 268 190 106 37
EcoNI CCTnn'n_nnAGG
Cuts at: 3209 3277 3209
Size: 68 6968
EcoO109I rG'GnC_Cy
Cuts at: 2040 2369 2459 2040
Size: 329 90 6617
Fragments arranged by size:
6617 329 90
EcoRI G'AATT_C
Cuts at: 2586 4345 4723 2586
Size: 1759 378 4899
Fragments arranged by size:
4899 1759 378
EcoRV GAT'ATC
Cuts at: 2754 3335 2754
Size: 581 6455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3824 3824
Size: 7036
FspI TGC'GCA
Cuts at: 5542 5542
Size: 7036
HaeII r_GCGC'y
Cuts at: 1678 6183 1678
Size: 4505 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2118 2150 3253 3285 3365
Size: 32 135 32 546 32 1103 32 80
Cuts at: 3365 3397 5677 5709 5751 5783 1373
Size: 32 2280 32 42 32 2626
Fragments arranged by size:
2626 2280 1103 546 135 80 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4072 4380 4626 669
Size: 988 281 2134 308 246 3079
Fragments arranged by size:
3079 2134 988 308 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7036
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 3578 4635 3578
Size: 1057 5979
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2420 6150 6334 1305
Size: 1115 3730 184 2007
Fragments arranged by size:
3730 2007 1115 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5214
Fragments arranged by size:
5214 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 2854 4994 5353 5512 518
Size: 648 1614 74 2140 359 159 2042
Fragments arranged by size:
2140 2042 1614 648 359 159 74
MspA1I CmG'CkG
Cuts at: 1606 3421 3993 4167 4452 5129 6277 6522 1606
Size: 1815 572 174 285 677 1148 245 2120
Fragments arranged by size:
2120 1815 1148 677 572 285 245 174
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6484
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7036
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7036
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NsiI A_TGCA'T
Cuts at: 2168 2168
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 2316 3178 4394 5939 1132
Size: 97 1087 862 1216 1545 2229
Fragments arranged by size:
2229 1545 1216 1087 862 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7036
PciI A'CATG_T
Cuts at: 2312 5935 2312
Size: 3623 3413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4302 1108
Size: 3194 3842
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PmlI CAC'GTG
Cuts at: 3947 3947
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2745 5105 5137 6618 6650 2713
Size: 32 2360 32 1481 32 3099
Fragments arranged by size:
3099 2360 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 2459 2040
Size: 419 6617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7036
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PstI C_TGCA'G
Cuts at: 830 2919 3442 4388 830
Size: 2089 523 946 3478
Fragments arranged by size:
3478 2089 946 523
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7036
PvuII CAG'CTG
Cuts at: 3421 3421
Size: 7036
SalI G'TCGA_C
Cuts at: 1936 4070 4378 1936
Size: 2134 308 4594
Fragments arranged by size:
4594 2134 308
SbfI CC_TGCA'GG
Cuts at: 4388 4388
Size: 7036
ScaI AGT'ACT
Cuts at: 5284 6996 5284
Size: 1712 5324
SfcI C'TryA_G
Cuts at: 826 1045 2915 3198 3438 3742 4233 4384 5519
Size: 219 1870 283 240 304 491 151 1135
Cuts at: 5519 6200 6391 826
Size: 681 191 1471
Fragments arranged by size:
1870 1471 1135 681 491 304 283 240
219 191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7036
SmaI CCC'GGG
Cuts at: 1872 4363 1872
Size: 2491 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3149 3229 5140 6041 6303
Size: 197 926 72 1134 80 1911 901 262
Cuts at: 6303 820
Size: 1553
Fragments arranged by size:
1911 1553 1134 926 901 262 197 80
72
SnaBI TAC'GTA
Cuts at: 493 4827 493
Size: 4334 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 4394 1132
Size: 3262 3774
SspI AAT'ATT
Cuts at: 5 52 3019 4960 5
Size: 47 2967 1941 2081
Fragments arranged by size:
2967 2081 1941 47
StuI AGG'CCT
Cuts at: 1517 4121 1517
Size: 2604 4432
StyI C'CwwG_G
Cuts at: 513 1065 2365 3812 4475 513
Size: 552 1300 1447 663 3074
Fragments arranged by size:
3074 1447 1300 663 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2982 3572 4010 5063 5080 5233 5418
Size: 1255 1402 590 438 1053 17 153 185
Cuts at: 5418 5863 325
Size: 445 1498
Fragments arranged by size:
1498 1402 1255 1053 590 445 438 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4152 5282 6994
Size: 274 80 33 51 1443 2175 1130 1712
Cuts at: 6994 96
Size: 138
Fragments arranged by size:
2175 1712 1443 1130 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 4034 4066 6996 7028 2749
Size: 32 1253 32 2930 32 2757
Fragments arranged by size:
2930 2757 1253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 3343 4372 3343
Size: 1029 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 2856 2831
Size: 25 7011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7036
XmaI C'CCGG_G
Cuts at: 1870 4361 1870
Size: 2491 4545
XmnI GAAnn'nnTTC
Cuts at: 3807 5165 3807
Size: 1358 5678
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI