Restriction map for
pFN21AE0175
(Circular) (Six-base) MAPSORT of: pFN21AE0175.seq Check: 6818 from: 1 to: 5608
pFN21AE0175 5608 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2054 2378 2447 2848 2054
Size: 324 69 401 4814
Fragments arranged by size:
4814 401 324 69
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2295 2561 2860 3671 5055 5323 1632
Size: 663 266 299 811 1384 268 1917
Fragments arranged by size:
1917 1384 811 663 299 268 266
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 2744 4337 2744
Size: 1593 4015
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2143 2840 3351 4923 2143
Size: 697 511 1572 2828
Fragments arranged by size:
2828 1572 697 511
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2480 2917 3143 3295 5404 1493
Size: 987 437 226 152 2109 1697
Fragments arranged by size:
2109 1697 987 437 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2933 1870
Size: 73 282 708 4545
Fragments arranged by size:
4545 708 282 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2767 2929 4384 618
Size: 325 217 627 747 233 162 1455 1842
Fragments arranged by size:
1842 1455 747 627 325 233 217 162
BanII G_rGCy'C
Cuts at: 1391 2013 2340 2927 1391
Size: 622 327 587 4072
Fragments arranged by size:
4072 622 587 327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2863 928
Size: 971 964 3673
Fragments arranged by size:
3673 971 964
BbvCI CC'TCA_GC
Cuts at: 2701 2701
Size: 5608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2518 3500 4710 2518
Size: 982 1210 3416
Fragments arranged by size:
3416 1210 982
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2711 2720 4219
Size: 107 122 71 631 605 1039 9 1499
Cuts at: 4219 136
Size: 1525
Fragments arranged by size:
1525 1499 1039 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2423 5603 2423
Size: 3180 2428
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2539 3669 4825 1790
Size: 749 1130 1156 2573
Fragments arranged by size:
2573 1156 1130 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 3339 4297
Size: 416 53 83 399 24 899 1023 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1023 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 4268 1239
Size: 1098 1931 2579
Fragments arranged by size:
2579 1931 1098
Bpu10I CC'TnA_GC
Cuts at: 2701 2701
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 3733 4598 4896 2366
Size: 1367 865 298 3078
Fragments arranged by size:
3078 1367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2116 3399 493
Size: 711 912 1283 2702
Fragments arranged by size:
2702 1283 912 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2853 2883 1178
Size: 30 165 30 1450 30 3903
Fragments arranged by size:
3903 1450 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 1186
Size: 1327 4281
BseYI C'CCAG_C
Cuts at: 2321 2339 2450 2611 4811 5307 2321
Size: 18 111 161 2200 496 2622
Fragments arranged by size:
2622 2200 496 161 111 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2412 1163
Size: 573 676 4359
Fragments arranged by size:
4359 676 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 3669 3754 4825 2013
Size: 1656 85 1071 2796
Fragments arranged by size:
2796 1656 1071 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2340 2539 2927 3669 3754 4825
Size: 399 223 327 199 388 742 85 1071
Cuts at: 4825 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 388 327 223 199
85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2378 2447 2848 2963 844
Size: 1210 324 69 401 115 3489
Fragments arranged by size:
3489 1210 401 324 115 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2670 3495 1394
Size: 429 847 825 3507
Fragments arranged by size:
3507 847 825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2423 2938 3305 3690 3707 5159 5245 5257
Size: 451 515 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 515 451 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 513
Size: 552 939 525 3592
Fragments arranged by size:
3592 939 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2621 2659 3124 3918 3938 2621
Size: 38 465 794 20 4291
Fragments arranged by size:
4291 794 465 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DraIII CAC_nnn'GTG
Cuts at: 2206 2206
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2174 4615 809
Size: 507 858 2441 1802
Fragments arranged by size:
2441 1802 858 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3055 3944
Size: 54 1425 50 275 18 891 334 889
Cuts at: 3944 5573 8
Size: 1629 43
Fragments arranged by size:
1629 1425 891 889 334 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2537 3538 1353
Size: 918 266 1001 3423
Fragments arranged by size:
3423 1001 918 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2011
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2337 2561 2860 3671 4268 5055
Size: 393 663 42 224 299 811 597 787
Cuts at: 5055 5323 1239
Size: 268 1524
Fragments arranged by size:
1524 811 787 663 597 393 299 268
224 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 2413 2696 2413
Size: 283 5325
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2151 4755 1678
Size: 473 2604 2531
Fragments arranged by size:
2604 2531 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2730 2762 2853 2885 4249
Size: 32 135 32 1158 32 91 32 1364
Cuts at: 4249 4281 4323 4355 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1364 1158 135 91 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3566 3925 4084 518
Size: 648 1302 1098 359 159 2042
Fragments arranged by size:
2042 1302 1098 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2127 2687 3024 3701 4849 5094 1606
Size: 521 560 337 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 560 521 337 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2195 2966 4511 1132
Size: 97 966 771 1545 2229
Fragments arranged by size:
2229 1545 966 771 97
PasI CC'CwG_GG
Cuts at: 1348 2678 1348
Size: 1330 4278
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 2206 1108
Size: 1035 63 4510
Fragments arranged by size:
4510 1035 63
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2687 2687
Size: 5608
SacI G_AGCT'C
Cuts at: 2013 2013
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SapI GCTCTTCn'nnn_
Cuts at: 2537 2537
Size: 5608
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SexAI A'CCwGG_T
Cuts at: 2202 2202
Size: 5608
SfcI C'TryA_G
Cuts at: 826 1045 2956 4091 4772 4963 826
Size: 219 1911 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2720 2720
Size: 5608
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 3712 4613 4875 820
Size: 197 926 402 1367 901 262 1553
Fragments arranged by size:
1553 1367 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 2116 3399 493
Size: 1623 1283 2702
Fragments arranged by size:
2702 1623 1283
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 2370 1517
Size: 853 4755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2215 2365 2709 3047 513
Size: 552 939 84 127 150 344 338 3074
Fragments arranged by size:
3074 939 552 344 338 150 127 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2522 2749 2780 3635 3652 3805 3990
Size: 1255 942 227 31 855 17 153 185
Cuts at: 3990 4435 325
Size: 445 1498
Fragments arranged by size:
1498 1255 942 855 445 227 185 153
31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 2851
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfoI SrfI SwaI