Restriction map for
pFN21AE0173
(Circular) (Six-base) MAPSORT of: pFN21AE0173.seq Check: 9733 from: 1 to: 5599
pFN21AE0173 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2431 2431
Size: 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2052 3409 278
Size: 53 83 186 936 516 1357 2468
Fragments arranged by size:
2468 1357 936 516 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2785 3662 5046 5314 1632
Size: 1153 877 1384 268 1917
Fragments arranged by size:
1917 1384 1153 877 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 2130 4498 5475 1203
Size: 927 2368 977 1327
Fragments arranged by size:
2368 1327 977 927
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AlwNI CAG_nnn'CTG
Cuts at: 2023 3342 4914 2023
Size: 1319 1572 2708
Fragments arranged by size:
2708 1572 1319
ApaI G_GGCC'C
Cuts at: 2708 2708
Size: 5599
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2908 3134 3286 5395 1493
Size: 1415 226 152 2109 1697
Fragments arranged by size:
2109 1697 1415 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2409 2929 3296 2409
Size: 520 367 4712
Fragments arranged by size:
4712 520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 2847 2920 4375 618
Size: 325 217 627 903 157 73 1455 1842
Fragments arranged by size:
1842 1455 903 627 325 217 157 73
BanII G_rGCy'C
Cuts at: 1391 2170 2189 2541 2708 2918 1391
Size: 779 19 352 167 210 4072
Fragments arranged by size:
4072 779 352 210 167 19
BbeI G_GCGC'C
Cuts at: 2694 2694
Size: 5599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4628
BbvCI CC'TCA_GC
Cuts at: 2682 2682
Size: 5599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2752 3491 4701 2752
Size: 739 1210 3650
Fragments arranged by size:
3650 1210 739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2712 2880 4210
Size: 107 122 71 631 605 1040 168 1330
Cuts at: 4210 136
Size: 1525
Fragments arranged by size:
1525 1330 1040 631 605 168 122 107
71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2708 3660 4816 1790
Size: 918 952 1156 2573
Fragments arranged by size:
2573 1156 952 918
BmgBI CAC'GTC
Cuts at: 1318 2566 1318
Size: 1248 4351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 2774 3330
Size: 416 53 83 399 24 975 382 556
Cuts at: 3330 4288 442
Size: 958 1753
Fragments arranged by size:
1753 975 958 556 416 399 382 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2555
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 2793 4259 1239
Size: 1233 321 1466 2579
Fragments arranged by size:
2579 1466 1233 321
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2647 3724 4589 4887 2647
Size: 1077 865 298 3359
Fragments arranged by size:
3359 1077 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2691 2697 3406
Size: 53 83 186 936 516 642 6 709
Cuts at: 3406 3788 4423 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 709 642 635 516 382 186
83 53 6
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 2553 2583 1178
Size: 30 165 30 678 30 442 30 4194
Fragments arranged by size:
4194 678 442 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2699 2833 1186
Size: 1363 150 134 3952
Fragments arranged by size:
3952 1363 150 134
BseYI C'CCAG_C
Cuts at: 4802 5298 4802
Size: 496 5103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2128 2846 1163
Size: 573 392 718 3916
Fragments arranged by size:
3916 718 573 392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3660 3745 4816 2541
Size: 1119 85 1071 3324
Fragments arranged by size:
3324 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
BsmBI CGTCTCn'nnnn_
Cuts at: 2556 2556
Size: 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2189 2541 2708 2918 3660 3745
Size: 399 380 19 352 167 210 742 85
Cuts at: 3745 4816 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 352 210 167
85 19
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 2954 844
Size: 1587 523 3489
Fragments arranged by size:
3489 1587 523
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2858 4243 4430 1700
Size: 110 1048 1385 187 2869
Fragments arranged by size:
2869 1385 1048 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 2529 3659 4671 2529
Size: 1130 1012 3457
Fragments arranged by size:
3457 1130 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2599 2552
Size: 47 5552
BstYI r'GATC_y
Cuts at: 1972 2019 2409 2499 2929 3296 3681 3698 5150
Size: 47 390 90 430 367 385 17 1452
Cuts at: 5150 5236 5248 5594 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 430 390 385 367 346 90
86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2076
Size: 5599
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 2511 513
Size: 552 939 291 216 3601
Fragments arranged by size:
3601 939 552 291 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2275 487
Size: 683 56 1049 3811
Fragments arranged by size:
3811 1049 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2851 2886 529
Size: 35 2287 35 3242
Fragments arranged by size:
3242 2287 35 35
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2589 2856
Size: 54 1425 50 275 18 370 389 267
Cuts at: 2856 3046 3935 5564 8
Size: 190 889 1629 43
Fragments arranged by size:
1629 1425 889 389 370 275 267 190
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2546 3529 1353
Size: 651 542 983 3423
Fragments arranged by size:
3423 983 651 542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 4186 4560 4706 387
Size: 1064 138 633 1964 374 146 1280
Fragments arranged by size:
1964 1280 1064 633 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 5599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2785 2793 3662 4259 5046 5314
Size: 393 840 313 8 869 597 787 268
Cuts at: 5314 1239
Size: 1524
Fragments arranged by size:
1524 869 840 787 597 393 313 268
8
EcoNI CCTnn'n_nnAGG
Cuts at: 2810 2810
Size: 5599
EcoO109I rG'GnC_Cy
Cuts at: 2705 2705
Size: 5599
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2694 4746 1678
Size: 1016 2052 2531
Fragments arranged by size:
2531 2052 1016
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373
Size: 32 135 32 2668 32 42 32 2626
Fragments arranged by size:
2668 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KasI G'GCGC_C
Cuts at: 2690 2690
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 2130 5475 2130
Size: 3345 2254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 4713 4897 1305
Size: 855 2553 184 2007
Fragments arranged by size:
2553 2007 855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2202 2591 10
Size: 54 1425 343 370 389 3018
Fragments arranged by size:
3018 1425 389 370 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 3557 3916 4075 518
Size: 648 1564 827 359 159 2042
Fragments arranged by size:
2042 1564 827 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2458 2548 3015 3692 4840 5085 1606
Size: 852 90 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 677 467 245 90
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 5599
NarI GG'CG_CC
Cuts at: 2691 2691
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4108
Fragments arranged by size:
4108 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2957 4502 1132
Size: 97 1728 1545 2229
Fragments arranged by size:
2229 1728 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2701 2812 1348
Size: 1353 111 4135
Fragments arranged by size:
4135 1353 111
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PspOMI G'GGCC_C
Cuts at: 2704 2704
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2457 2880 2951 830
Size: 1627 423 71 3478
Fragments arranged by size:
3478 1627 423 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2548 2548
Size: 5599
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SapI GCTCTTCn'nnn_
Cuts at: 2546 2546
Size: 5599
SbfI CC_TGCA'GG
Cuts at: 2880 2951 2880
Size: 71 5528
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2370 2453 2876 2947 4082 4763 4954
Size: 219 1325 83 423 71 1135 681 191
Cuts at: 4954 826
Size: 1471
Fragments arranged by size:
1471 1325 1135 681 423 219 191 83
71
SfoI GGC'GCC
Cuts at: 2692 2692
Size: 5599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2626 3703 4604 4866 820
Size: 197 926 683 1077 901 262 1553
Fragments arranged by size:
1553 1077 926 901 683 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 2004 3038 513
Size: 552 939 1034 3074
Fragments arranged by size:
3074 1034 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3626 3643 3796 3981 4426 325
Size: 1255 2046 17 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 2553 2585 5559 5591 2081
Size: 32 440 32 2974 32 2089
Fragments arranged by size:
2974 2089 440 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2050 3407 276
Size: 53 83 186 936 516 1357 2468
Fragments arranged by size:
2468 1357 936 516 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI