Restriction map for
pFN21AE0172
(Circular) (Six-base) MAPSORT of: pFN21AE0172.seq Check: 692 from: 1 to: 5656
pFN21AE0172 5656 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2846 3466 278
Size: 53 83 186 936 1310 620 2468
Fragments arranged by size:
2468 1310 936 620 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 2999 1937
Size: 664 398 4594
Fragments arranged by size:
4594 664 398
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2440 820
Size: 197 1423 4036
Fragments arranged by size:
4036 1423 197
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2519 3399 4971 2519
Size: 880 1572 3204
Fragments arranged by size:
3204 1572 880
ApaI G_GGCC'C
Cuts at: 2129 2129
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2138 2965 3191 3343 5452 1493
Size: 645 827 226 152 2109 1697
Fragments arranged by size:
2109 1697 827 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2815 2977 4432 618
Size: 325 217 627 1028 162 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2129 2904 2975 1391
Size: 738 775 71 4072
Fragments arranged by size:
4072 775 738 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2296 928
Size: 971 397 4288
Fragments arranged by size:
4288 971 397
BbvCI CC'TCA_GC
Cuts at: 2421 2421
Size: 5656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2129 3717 4873 1790
Size: 339 1588 1156 2573
Fragments arranged by size:
2573 1588 1156 339
BmgBI CAC'GTC
Cuts at: 1318 2042 1318
Size: 724 4932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 4316 1239
Size: 1262 1815 2579
Fragments arranged by size:
2579 1815 1262
Bpu10I CC'TnA_GC
Cuts at: 2421 2421
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2564 5650 2564
Size: 3086 2570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2843 3463 3845 4480
Size: 53 83 186 936 1310 620 382 635
Cuts at: 4480 275
Size: 1451
Fragments arranged by size:
1451 1310 936 635 620 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2448 4089 4487 4761 4908 5319 2448
Size: 1641 398 274 147 411 2785
Fragments arranged by size:
2785 1641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2362 2408 1186
Size: 1176 46 4434
Fragments arranged by size:
4434 1176 46
BseYI C'CCAG_C
Cuts at: 2922 4859 5355 2922
Size: 1937 496 3223
Fragments arranged by size:
3223 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1996 2822 1163
Size: 573 260 826 3997
Fragments arranged by size:
3997 826 573 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2234 3717 3802 4873 2234
Size: 1483 85 1071 3017
Fragments arranged by size:
3017 1483 1071 85
BsiWI C'GTAC_G
Cuts at: 2130 2130
Size: 5656
BsmI GAATG_Cn'
Cuts at: 2831 3167 3260 2831
Size: 336 93 5227
Fragments arranged by size:
5227 336 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2850 2850
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2234 2904 2975 3717 3802 4873
Size: 399 339 105 670 71 742 85 1071
Cuts at: 4873 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 670 399 339 105 85
71
BspEI T'CCGG_A
Cuts at: 2448 5319 2448
Size: 2871 2785
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2390 3716 4728 2390
Size: 1326 1012 3318
Fragments arranged by size:
3318 1326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2726 2726
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2035 2335 2559 2986 3353 3738 3755 5207
Size: 63 300 224 427 367 385 17 1452
Cuts at: 5207 5293 5305 5651 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 427 385 367 346 300 224
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 513
Size: 552 1002 4102
Fragments arranged by size:
4102 1002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 3172 3966 3986 2289
Size: 883 794 20 3959
Fragments arranged by size:
3959 883 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2028 2063 529
Size: 35 1464 35 4122
Fragments arranged by size:
4122 1464 35 35
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 3103 3992
Size: 54 1425 50 275 18 246 1027 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1027 889 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2909 3586 1353
Size: 755 801 677 3423
Fragments arranged by size:
3423 801 755 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 3719 4316 5103 5371 1239
Size: 393 869 1218 597 787 268 1524
Fragments arranged by size:
1524 1218 869 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
EcoRV GAT'ATC
Cuts at: 2368 2368
Size: 5656
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 2636 2668 2849
Size: 32 135 32 785 32 247 32 181
Cuts at: 2849 2881 4297 4329 4371 4403 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 785 247 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3000 3246 669
Size: 988 281 930 132 246 3079
Fragments arranged by size:
3079 988 930 281 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2334 2708 4770 4954 1305
Size: 1029 374 2062 184 2007
Fragments arranged by size:
2062 2007 1029 374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 10
Size: 54 1425 343 246 3588
Fragments arranged by size:
3588 1425 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2066 2724 3614 3973 4132 518
Size: 648 852 48 658 890 359 159 2042
Fragments arranged by size:
2042 890 852 658 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 3725 3757 5238 5270 2930
Size: 32 763 32 1481 32 3316
Fragments arranged by size:
3316 1481 763 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2125 2125
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2343 3008 830
Size: 1513 665 3478
Fragments arranged by size:
3478 1513 665
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SapI GCTCTTCn'nnn_
Cuts at: 2909 2909
Size: 5656
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2339 3004 4139 4820 5011 826
Size: 219 1294 665 1135 681 191 1471
Fragments arranged by size:
1471 1294 1135 681 665 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2440 3760 4661 4923 820
Size: 197 926 497 1320 901 262 1553
Fragments arranged by size:
1553 1320 926 901 497 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2274 3095 513
Size: 552 1209 821 3074
Fragments arranged by size:
3074 1209 821 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2047 3683 3700 3853 4038 4483 325
Size: 1255 467 1636 17 153 185 445 1498
Fragments arranged by size:
1636 1498 1255 467 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 2606 1316
Size: 1290 4366
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2117 3785 2117
Size: 1668 3988
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2844 3464 276
Size: 53 83 186 936 1310 620 2468
Fragments arranged by size:
2468 1310 936 620 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI