Restriction map for
pFN21AE0166
(Circular) (Six-base) MAPSORT of: pFN21AE0166.seq Check: 9093 from: 1 to: 5449
pFN21AE0166 5449 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AfeI AGC'GCT
Cuts at: 2080 2674 2080
Size: 594 4855
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 2526 4178 2526
Size: 1652 3797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 1184
Size: 1236 4213
AlwNI CAG_nnn'CTG
Cuts at: 3192 4764 3192
Size: 1572 3877
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 1870
Size: 73 831 4545
Fragments arranged by size:
4545 831 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2770 4225 618
Size: 325 217 627 983 1455 1842
Fragments arranged by size:
1842 1455 983 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 2768 1391
Size: 709 668 4072
Fragments arranged by size:
4072 709 668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4478
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4060 136
Size: 107 122 71 631 605 2388 1525
Fragments arranged by size:
2388 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 3510 4666 1790
Size: 1720 1156 2573
Fragments arranged by size:
2573 1720 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2418 2475 3180
Size: 416 53 83 399 24 1001 57 705
Cuts at: 3180 4138 442
Size: 958 1753
Fragments arranged by size:
1753 1001 958 705 416 399 83 57
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2334 2556 2673 4109 1239
Size: 1086 9 222 117 1436 2579
Fragments arranged by size:
2579 1436 1086 222 117 9
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 5449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2293 3574 4439 4737 2054
Size: 239 1281 865 298 2766
Fragments arranged by size:
2766 1281 865 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2137 2167 1178
Size: 30 165 30 734 30 4460
Fragments arranged by size:
4460 734 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 1186
Size: 894 4555
BseYI C'CCAG_C
Cuts at: 4652 5148 4652
Size: 496 4953
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2199 1163
Size: 573 463 4413
Fragments arranged by size:
4413 573 463
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3510 3595 4666 3510
Size: 85 1071 4293
Fragments arranged by size:
4293 1071 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2768 3510 3595 4666 1391
Size: 399 310 668 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 399 310 85
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 3944 4118 65
Size: 1183 999 1697 174 1396
Fragments arranged by size:
1697 1396 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2069 2779 3146 3531 3548 5000 5086 5098
Size: 97 710 367 385 17 1452 86 12
Cuts at: 5098 5444 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 97 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2063 2063
Size: 5449
BtgI C'CryG_G
Cuts at: 513 1065 2004 2092 513
Size: 552 939 88 3870
Fragments arranged by size:
3870 939 552 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 2644 2965 3759 3779 2404
Size: 240 321 794 20 4074
Fragments arranged by size:
4074 794 321 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2896 3785 5414
Size: 54 1425 50 275 18 1066 889 1629
Cuts at: 5414 8
Size: 43
Fragments arranged by size:
1629 1425 1066 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 2719 3379 1353
Size: 1196 170 660 3423
Fragments arranged by size:
3423 1196 660 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 2334 2556 2673 3512 4109 4896
Size: 393 693 9 222 117 839 597 787
Cuts at: 4896 5164 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 693 597 393 268 222
117 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2111
Size: 5449
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5449
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 2082 2676 4596 1678
Size: 404 594 1920 2531
Fragments arranged by size:
2531 1920 594 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 2719 2723 2751
Size: 32 135 32 940 32 175 4 28
Cuts at: 2751 2755 4090 4122 4164 4196 1373
Size: 4 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 940 175 135 42 32 32
32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2146 4563 4747 1305
Size: 841 2417 184 2007
Fragments arranged by size:
2417 2007 841 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3627
Fragments arranged by size:
3627 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 3407 3766 3925 518
Size: 648 1560 681 359 159 2042
Fragments arranged by size:
2042 1560 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2659 2865 3542 4690 4935 1606
Size: 1053 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 245 206
NcoI C'CATG_G
Cuts at: 513 1065 2004 2092 513
Size: 552 939 88 3870
Fragments arranged by size:
3870 939 552 88
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2700 2807 4352 1132
Size: 97 1471 107 1545 2229
Fragments arranged by size:
2229 1545 1471 107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2801 830
Size: 1971 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SapI GCTCTTCn'nnn_
Cuts at: 2719 2719
Size: 5449
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2797 3932 4613 4804 826
Size: 219 1752 1135 681 191 1471
Fragments arranged by size:
1752 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 2272 3553 4454 4716 820
Size: 197 926 90 239 1281 901 262 1553
Fragments arranged by size:
1553 1281 926 901 262 239 197 90
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2700 2807 1132
Size: 1568 107 3774
Fragments arranged by size:
3774 1568 107
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 2190 1517
Size: 673 4776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2092 2548 2745 2888 513
Size: 552 939 88 456 197 143 3074
Fragments arranged by size:
3074 939 552 456 197 143 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2377 2758 3476 3493 3646 3831 4276
Size: 1255 797 381 718 17 153 185 445
Cuts at: 4276 325
Size: 1498
Fragments arranged by size:
1498 1255 797 718 445 381 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2153 3695 5407
Size: 274 80 33 51 1443 176 1542 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1542 1443 274 176 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 5409 5441 2103
Size: 32 3274 32 2111
Fragments arranged by size:
3274 2111 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 3578 3578
Size: 5449
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI