Restriction map for
pFN21AE0165
(Circular) (Six-base) MAPSORT of: pFN21AE0165.seq Check: 8889 from: 1 to: 5266
pFN21AE0165 5266 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3076 278
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2609 1937
Size: 672 4594
Acc65I G'GTAC_C
Cuts at: 2587 2587
Size: 5266
AclI AA'CG_TT
Cuts at: 1563 3393 3766 1563
Size: 1830 373 3063
Fragments arranged by size:
3063 1830 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2315 2321 3329 4713 4981 1632
Size: 446 237 6 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1008 446 268 237 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5069
AflIII A'CryG_T
Cuts at: 1203 4165 5142 1203
Size: 2962 977 1327
Fragments arranged by size:
2962 1327 977
AgeI A'CCGG_T
Cuts at: 4097 4097
Size: 5266
AhdI GACnn_n'nnGTC
Cuts at: 3995 3995
Size: 5266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 1184
Size: 885 4381
AlwNI CAG_nnn'CTG
Cuts at: 2197 3009 4581 2197
Size: 812 1572 2882
Fragments arranged by size:
2882 1572 812
ApaI G_GGCC'C
Cuts at: 2383 2383
Size: 5266
ApaLI G'TGCA_C
Cuts at: 3323 4479 3323
Size: 1156 4110
ApoI r'AATT_y
Cuts at: 1493 2575 2801 2953 5062 1493
Size: 1082 226 152 2109 1697
Fragments arranged by size:
2109 1697 1082 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3820 160
Size: 872 2788 1606
Fragments arranged by size:
2788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
AvaI C'yCGr_G
Cuts at: 1870 1943 2591 1870
Size: 73 648 4545
Fragments arranged by size:
4545 648 73
BamHI G'GATC_C
Cuts at: 2596 2963 2596
Size: 367 4899
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2587 4042 618
Size: 325 217 627 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2383 2393 2585 1391
Size: 992 10 192 4072
Fragments arranged by size:
4072 992 192 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 928
Size: 971 555 3740
Fragments arranged by size:
3740 971 555
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3457 3491 1903
Size: 34 50 34 1436 34 3678
Fragments arranged by size:
3678 1436 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3158 4368 3158
Size: 1210 4056
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5266
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3877 136
Size: 107 122 71 631 605 2205 1525
Fragments arranged by size:
2205 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5261 5261
Size: 5266
BlpI GC'TnA_GC
Cuts at: 2683 2683
Size: 5266
Bme1580I G_kGCm'C
Cuts at: 1790 2188 2383 3327 4483 1790
Size: 398 195 944 1156 2573
Fragments arranged by size:
2573 1156 944 398 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2997 3955 442
Size: 416 53 83 399 24 1580 958 1753
Fragments arranged by size:
1753 1580 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2506 3926 1239
Size: 1267 1420 2579
Fragments arranged by size:
2579 1420 1267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3391 4256 4554 2414
Size: 977 865 298 3126
Fragments arranged by size:
3126 977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3929 882
Size: 617 2430 2219
Fragments arranged by size:
2430 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3057 493
Size: 711 1853 2702
Fragments arranged by size:
2702 1853 711
BsaBI GATnn'nnATC
Cuts at: 5260 5260
Size: 5266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3073 3455 4090
Size: 53 83 186 936 834 706 382 635
Cuts at: 4090 275
Size: 1451
Fragments arranged by size:
1451 936 834 706 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3699 4097 4371 4518 4929 3699
Size: 398 274 147 411 4036
Fragments arranged by size:
4036 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 3922
Fragments arranged by size:
3922 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2252 1186
Size: 937 129 4200
Fragments arranged by size:
4200 937 129
BseYI C'CCAG_C
Cuts at: 2091 2386 4469 4965 2091
Size: 295 2083 496 2392
Fragments arranged by size:
2392 2083 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 4234
Fragments arranged by size:
4234 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2716 3477 4505 1540
Size: 275 186 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 3327 3412 4483 2106
Size: 1221 85 1071 2889
Fragments arranged by size:
2889 1221 1071 85
BsmI GAATG_Cn'
Cuts at: 2093 2777 2870 2093
Size: 684 93 4489
Fragments arranged by size:
4489 684 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2188 2383 2393 2585 3327 3412
Size: 399 316 82 195 10 192 742 85
Cuts at: 3412 4483 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 195 192 85
82 10
BspEI T'CCGG_A
Cuts at: 4929 4929
Size: 5266
BspHI T'CATG_A
Cuts at: 3155 4885 3155
Size: 1730 3536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2621 844
Size: 1777 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2464 3153 1394
Size: 429 641 689 3507
Fragments arranged by size:
3507 689 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3761 3935 65
Size: 1183 2513 174 1396
Fragments arranged by size:
2513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3910 4097 1700
Size: 110 2100 187 2869
Fragments arranged by size:
2869 2100 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5266
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5266
BssSI C'ACGA_G
Cuts at: 2105 3326 4338 2105
Size: 1221 1012 3033
Fragments arranged by size:
3033 1221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5266
BstBI TT'CG_AA
Cuts at: 4070 4070
Size: 5266
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5266
BstYI r'GATC_y
Cuts at: 1972 2413 2596 2963 3348 3365 4817 4903 4915
Size: 441 183 367 385 17 1452 86 12
Cuts at: 4915 5261 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 441 385 367 346 183 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2167 2317 2409 513
Size: 552 1102 150 92 3370
Fragments arranged by size:
3370 1102 552 150 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4527
Fragments arranged by size:
4527 683 56
BtsI GCAGTG_nn'
Cuts at: 2034 2782 3576 3596 2034
Size: 748 794 20 3704
Fragments arranged by size:
3704 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5231
DraI TTT'AAA
Cuts at: 2570 2570
Size: 5266
DraIII CAC_nnn'GTG
Cuts at: 2404 2404
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4273 809
Size: 507 2957 1802
Fragments arranged by size:
2957 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2713 3602
Size: 54 1425 50 275 18 243 640 889
Cuts at: 3602 5231 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 640 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2713 1537
Size: 275 901 4090
Fragments arranged by size:
4090 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3196 1353
Size: 701 1142 3423
Fragments arranged by size:
3423 1142 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3853 4227 4373 387
Size: 1064 138 2264 374 146 1280
Fragments arranged by size:
2264 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2315 2321 2506 3329 3926 4713
Size: 393 446 237 6 185 823 597 787
Cuts at: 4713 4981 1239
Size: 268 1524
Fragments arranged by size:
1524 823 787 597 446 393 268 237
185 6
EcoO109I rG'GnC_Cy
Cuts at: 2194 2290 2380 2194
Size: 96 90 5080
Fragments arranged by size:
5080 96 90
EcoRI G'AATT_C
Cuts at: 2575 2953 2575
Size: 378 4888
FspI TGC'GCA
Cuts at: 3772 3772
Size: 5266
HaeII r_GCGC'y
Cuts at: 1678 2335 4413 1678
Size: 657 2078 2531
Fragments arranged by size:
2531 2078 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2340 2372 2492 2524 3907
Size: 32 135 32 768 32 120 32 1383
Cuts at: 3907 3939 3981 4013 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1383 768 135 120 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2610 2856 669
Size: 988 281 672 246 3079
Fragments arranged by size:
3079 988 672 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5266
HpaI GTT'AAC
Cuts at: 2856 2856
Size: 5266
KpnI G_GTAC'C
Cuts at: 2591 2591
Size: 5266
MfeI C'AATT_G
Cuts at: 2865 2865
Size: 5266
MluI A'CGCG_T
Cuts at: 5142 5142
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2579 4380 4564 1305
Size: 1274 1801 184 2007
Fragments arranged by size:
2007 1801 1274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 10
Size: 54 1425 343 243 3201
Fragments arranged by size:
3201 1425 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3224 3583 3742 518
Size: 648 2058 359 159 2042
Fragments arranged by size:
2058 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2682 3359 4507 4752 1606
Size: 1076 677 1148 245 2120
Fragments arranged by size:
2120 1148 1076 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2409 513
Size: 552 1344 3370
Fragments arranged by size:
3370 1344 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5266
NotI GC'GGCC_GC
Cuts at: 2713 2713
Size: 5266
NspI r_CATG'y
Cuts at: 1132 1229 2624 4169 1132
Size: 97 1395 1545 2229
Fragments arranged by size:
2229 1545 1395 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5266
PciI A'CATG_T
Cuts at: 4165 4165
Size: 5266
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2570 2570
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 4848 4880 3335
Size: 32 1481 32 3721
Fragments arranged by size:
3721 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2290 2194
Size: 96 5170
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5266
PsiI TTA'TAA
Cuts at: 2836 2836
Size: 5266
PspOMI G'GGCC_C
Cuts at: 2379 2379
Size: 5266
PstI C_TGCA'G
Cuts at: 830 2163 2193 2618 830
Size: 1333 30 425 3478
Fragments arranged by size:
3478 1333 425 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5266
SalI G'TCGA_C
Cuts at: 1936 2608 1936
Size: 672 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5266
SbfI CC_TGCA'GG
Cuts at: 2193 2618 2193
Size: 425 4841
ScaI AGT'ACT
Cuts at: 3514 5226 3514
Size: 1712 3554
SfcI C'TryA_G
Cuts at: 826 1045 2159 2189 2614 3749 4430 4621 826
Size: 219 1114 30 425 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 425 219 191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5266
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5266
SmaI CCC'GGG
Cuts at: 1872 2593 1872
Size: 721 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3370 4271 4533 820
Size: 197 926 486 941 901 262 1553
Fragments arranged by size:
1553 941 926 901 486 262 197
SnaBI TAC'GTA
Cuts at: 493 3057 493
Size: 2564 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5266
SphI G_CATG'C
Cuts at: 1132 2624 1132
Size: 1492 3774
SspI AAT'ATT
Cuts at: 5 52 3190 5
Size: 47 3138 2081
Fragments arranged by size:
3138 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5266
StyI C'CwwG_G
Cuts at: 513 1065 2076 2082 2217 2409 2705 513
Size: 552 1011 6 135 192 296 3074
Fragments arranged by size:
3074 1011 552 296 192 135 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 3293 3310 3463 3648 4093 325
Size: 1255 888 825 17 153 185 445 1498
Fragments arranged by size:
1498 1255 888 825 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3512 5224 96
Size: 274 80 33 51 1443 1535 1712 138
Fragments arranged by size:
1712 1535 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2390 2422 5226 5258 2390
Size: 32 2804 32 2398
Fragments arranged by size:
2804 2398 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5266
XbaI T'CTAG_A
Cuts at: 2602 2602
Size: 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 2512 2224
Size: 288 4978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5266
XmaI C'CCGG_G
Cuts at: 1870 2591 1870
Size: 721 4545
XmnI GAAnn'nnTTC
Cuts at: 3395 3395
Size: 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3074 276
Size: 53 83 186 936 1540 2468
Fragments arranged by size:
2468 1540 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI