Restriction map for
pFN21AE0163
(Circular) (Six-base) MAPSORT of: pFN21AE0163.seq Check: 15 from: 1 to: 5044
pFN21AE0163 5044 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2854 278
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2387 1937
Size: 450 4594
Acc65I G'GTAC_C
Cuts at: 2112 2365 2112
Size: 253 4791
AclI AA'CG_TT
Cuts at: 1563 3171 3544 1563
Size: 1608 373 3063
Fragments arranged by size:
3063 1608 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3107 4491 4759 1632
Size: 1475 1384 268 1917
Fragments arranged by size:
1917 1475 1384 268
AfeI AGC'GCT
Cuts at: 2270 2270
Size: 5044
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4847
AflIII A'CryG_T
Cuts at: 1203 2230 3943 4920 1203
Size: 1027 1713 977 1327
Fragments arranged by size:
1713 1327 1027 977
AgeI A'CCGG_T
Cuts at: 3875 3875
Size: 5044
AhdI GACnn_n'nnGTC
Cuts at: 3773 3773
Size: 5044
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5044
AlwNI CAG_nnn'CTG
Cuts at: 2787 4359 2787
Size: 1572 3472
ApaLI G'TGCA_C
Cuts at: 2133 3101 4257 2133
Size: 968 1156 2920
Fragments arranged by size:
2920 1156 968
ApoI r'AATT_y
Cuts at: 1493 2249 2353 2579 2731 4840 1493
Size: 756 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 3598 160
Size: 872 2566 1606
Fragments arranged by size:
2566 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
AvaI C'yCGr_G
Cuts at: 1870 1943 2369 1870
Size: 73 426 4545
Fragments arranged by size:
4545 426 73
BamHI G'GATC_C
Cuts at: 2374 2741 2374
Size: 367 4677
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2365 3820 618
Size: 325 217 627 325 253 1455 1842
Fragments arranged by size:
1842 1455 627 325 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2170 2363 1391
Size: 779 193 4072
Fragments arranged by size:
4072 779 193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2018 2052 3235 3269 1903
Size: 34 81 34 1183 34 3678
Fragments arranged by size:
3678 1183 81 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 4146 2936
Size: 1210 3834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3655 136
Size: 107 122 71 631 605 337 1646 1525
Fragments arranged by size:
1646 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5039 5039
Size: 5044
BlpI GC'TnA_GC
Cuts at: 2461 2461
Size: 5044
Bme1580I G_kGCm'C
Cuts at: 1790 2137 3105 4261 1790
Size: 347 968 1156 2573
Fragments arranged by size:
2573 1156 968 347
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5044
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2775 3733 442
Size: 416 53 83 399 24 1358 958 1753
Fragments arranged by size:
1753 1358 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2343 3704 1239
Size: 1104 1361 2579
Fragments arranged by size:
2579 1361 1104
Bpu10I CC'TnA_GC
Cuts at: 2066 2066
Size: 5044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 3169 4034 4332 2288
Size: 881 865 298 3000
Fragments arranged by size:
3000 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 3707 882
Size: 617 807 1401 2219
Fragments arranged by size:
2219 1401 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 2835 493
Size: 711 1631 2702
Fragments arranged by size:
2702 1631 711
BsaBI GATnn'nnATC
Cuts at: 5038 5038
Size: 5044
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2851 3233 3868 275
Size: 53 83 186 936 1318 382 635 1451
Fragments arranged by size:
1451 1318 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2223 3477 3875 4149 4296 4707 2223
Size: 1254 398 274 147 411 2560
Fragments arranged by size:
2560 1254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4819
Fragments arranged by size:
4819 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 1186
Size: 1108 3936
BseYI C'CCAG_C
Cuts at: 2334 4247 4743 2334
Size: 1913 496 2635
Fragments arranged by size:
2635 1913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2182 1163
Size: 573 446 4025
Fragments arranged by size:
4025 573 446
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 2494 3255 4283 1540
Size: 275 186 82 411 761 1028 2301
Fragments arranged by size:
2301 1028 761 411 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 2137 3105 3190 4261 2137
Size: 968 85 1071 2920
Fragments arranged by size:
2920 1071 968 85
BsmI GAATG_Cn'
Cuts at: 2555 2648 2555
Size: 93 4951
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2170 2363 3105 3190 4261 1391
Size: 399 347 33 193 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 193 85 33
BspEI T'CCGG_A
Cuts at: 2223 4707 2223
Size: 2484 2560
BspHI T'CATG_A
Cuts at: 2933 4663 2933
Size: 1730 3314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2399 844
Size: 1555 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2931 1394
Size: 429 1108 3507
Fragments arranged by size:
3507 1108 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3539 3713 65
Size: 1183 2291 174 1396
Fragments arranged by size:
2291 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 3688 3875 1700
Size: 110 268 1610 187 2869
Fragments arranged by size:
2869 1610 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5044
BssSI C'ACGA_G
Cuts at: 3104 4116 3104
Size: 1012 4032
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5044
BstBI TT'CG_AA
Cuts at: 3848 3848
Size: 5044
BstEII G'GTnAC_C
Cuts at: 1184 2143 1184
Size: 959 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 2321 2321
Size: 5044
BstYI r'GATC_y
Cuts at: 1972 2374 2741 3126 3143 4595 4681 4693 5039
Size: 402 367 385 17 1452 86 12 346
Cuts at: 5039 1972
Size: 1977
Fragments arranged by size:
1977 1452 402 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2014 2014
Size: 5044
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 513
Size: 552 939 96 3457
Fragments arranged by size:
3457 939 552 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4305
Fragments arranged by size:
4305 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 3354 3374 2560
Size: 794 20 4230
Fragments arranged by size:
4230 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5009
DraI TTT'AAA
Cuts at: 2348 2348
Size: 5044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4051 809
Size: 507 2735 1802
Fragments arranged by size:
2735 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2080 2491
Size: 54 1425 50 275 18 243 7 411
Cuts at: 2491 3380 5009 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 411 275 243 54 50
43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2080 2491 1537
Size: 275 268 411 4090
Fragments arranged by size:
4090 411 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 2974 1353
Size: 1621 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3631 4005 4151 387
Size: 1064 138 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2343 3107 3704 4491 4759 1239
Size: 393 711 764 597 787 268 1524
Fragments arranged by size:
1524 787 764 711 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2243 2120
Size: 123 4921
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 5044
EcoRI G'AATT_C
Cuts at: 2353 2731 2353
Size: 378 4666
FspI TGC'GCA
Cuts at: 3550 3550
Size: 5044
HaeII r_GCGC'y
Cuts at: 1678 2272 4191 1678
Size: 594 1919 2531
Fragments arranged by size:
2531 1919 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3685 3717 3759 3791 1373
Size: 32 135 32 2113 32 42 32 2626
Fragments arranged by size:
2626 2113 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2388 2634 669
Size: 988 281 450 246 3079
Fragments arranged by size:
3079 988 450 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5044
HpaI GTT'AAC
Cuts at: 2634 2634
Size: 5044
KpnI G_GTAC'C
Cuts at: 2116 2369 2116
Size: 253 4791
MfeI C'AATT_G
Cuts at: 2643 2643
Size: 5044
MluI A'CGCG_T
Cuts at: 4920 4920
Size: 5044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4158 4342 1305
Size: 2853 184 2007
Fragments arranged by size:
2853 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3222
Fragments arranged by size:
3222 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3002 3361 3520 518
Size: 648 1836 359 159 2042
Fragments arranged by size:
2042 1836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2460 3137 4285 4530 1606
Size: 496 358 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 496 358 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3553
Fragments arranged by size:
3553 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5044
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5044
NotI GC'GGCC_GC
Cuts at: 2491 2491
Size: 5044
NruI TCG'CGA
Cuts at: 2094 2094
Size: 5044
NspI r_CATG'y
Cuts at: 1132 1229 2234 2402 3947 1132
Size: 97 1005 168 1545 2229
Fragments arranged by size:
2229 1545 1005 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5044
PciI A'CATG_T
Cuts at: 2230 3943 2230
Size: 1713 3331
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5044
PmeI GTTT'AAAC
Cuts at: 2348 2348
Size: 5044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4626 4658 3113
Size: 32 1481 32 3499
Fragments arranged by size:
3499 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 2019
Size: 5044
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5044
PsiI TTA'TAA
Cuts at: 2614 2614
Size: 5044
PstI C_TGCA'G
Cuts at: 830 2396 830
Size: 1566 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5044
SacII CC_GC'GG
Cuts at: 2103 2103
Size: 5044
SalI G'TCGA_C
Cuts at: 1936 2386 1936
Size: 450 4594
SanDI GG'GwC_CC
Cuts at: 2019 2019
Size: 5044
SbfI CC_TGCA'GG
Cuts at: 2396 2396
Size: 5044
ScaI AGT'ACT
Cuts at: 3292 5004 3292
Size: 1712 3332
SfcI C'TryA_G
Cuts at: 826 1045 2392 3527 4208 4399 826
Size: 219 1347 1135 681 191 1471
Fragments arranged by size:
1471 1347 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5044
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5044
SmaI CCC'GGG
Cuts at: 1872 2371 1872
Size: 499 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 3148 4049 4311 820
Size: 197 926 360 845 901 262 1553
Fragments arranged by size:
1553 926 901 845 360 262 197
SnaBI TAC'GTA
Cuts at: 493 2835 493
Size: 2342 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5044
SphI G_CATG'C
Cuts at: 1132 2402 1132
Size: 1270 3774
SspI AAT'ATT
Cuts at: 5 52 2968 5
Size: 47 2916 2081
Fragments arranged by size:
2916 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5044
StyI C'CwwG_G
Cuts at: 513 1065 2004 2483 513
Size: 552 939 479 3074
Fragments arranged by size:
3074 939 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3071 3088 3241 3426 3871 325
Size: 1255 1491 17 153 185 445 1498
Fragments arranged by size:
1498 1491 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3290 5002 96
Size: 274 80 33 51 1443 1313 1712 138
Fragments arranged by size:
1712 1443 1313 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5004 5036 5004
Size: 32 5012
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5044
XbaI T'CTAG_A
Cuts at: 2380 2380
Size: 5044
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5044
XmaI C'CCGG_G
Cuts at: 1870 2369 1870
Size: 499 4545
XmnI GAAnn'nnTTC
Cuts at: 2126 3173 2126
Size: 1047 3997
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2852 276
Size: 53 83 186 936 1318 2468
Fragments arranged by size:
2468 1318 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI