Restriction map for
pFN21AE0162
(Circular) (Six-base) MAPSORT of: pFN21AE0162.seq Check: 9011 from: 1 to: 5635
pFN21AE0162 5635 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2197 2919 3698 5082 5350 1632
Size: 449 116 722 779 1384 268 1917
Fragments arranged by size:
1917 1384 779 722 449 268 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 2660 4364 2660
Size: 1704 3931
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2878 2910 2878
Size: 32 5603
AlwNI CAG_nnn'CTG
Cuts at: 2180 2257 2579 2819 3378 4950 2180
Size: 77 322 240 559 1572 2865
Fragments arranged by size:
2865 1572 559 322 240 77
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2822 2944 3170 3322 5431 1493
Size: 1329 122 226 152 2109 1697
Fragments arranged by size:
2109 1697 1329 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2960 1870
Size: 73 349 668 4545
Fragments arranged by size:
4545 668 349 73
BamHI G'GATC_C
Cuts at: 2686 2965 3332 2686
Size: 279 367 4989
Fragments arranged by size:
4989 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2355 2453 2956 4411 618
Size: 325 217 627 568 98 503 1455 1842
Fragments arranged by size:
1842 1455 627 568 503 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2346 2721 2954 1391
Size: 955 375 233 4072
Fragments arranged by size:
4072 955 375 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3826 3860 1903
Size: 34 50 34 1805 34 3678
Fragments arranged by size:
3678 1805 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2358 2753 3696 4852 1790
Size: 568 395 943 1156 2573
Fragments arranged by size:
2573 1156 943 568 395
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2597 3366 4324
Size: 416 53 83 399 24 1180 769 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1180 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 2052 2244 2489 2556 2720 4295 1239
Size: 761 52 192 245 67 164 1575 2579
Fragments arranged by size:
2579 1575 761 245 192 164 67 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2571 3760 4625 4923 2571
Size: 1189 865 298 3283
Fragments arranged by size:
3283 1189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 2828 4298 882
Size: 617 873 456 1470 2219
Fragments arranged by size:
2219 1470 873 617 456
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 1178
Size: 30 165 30 766 30 4614
Fragments arranged by size:
4614 766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2232 2717 1186
Size: 1046 485 4104
Fragments arranged by size:
4104 1046 485
BseYI C'CCAG_C
Cuts at: 2043 2386 2770 2842 4838 5334 2043
Size: 343 384 72 1996 496 2344
Fragments arranged by size:
2344 1996 496 384 343 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 2423 1163
Size: 573 475 212 4375
Fragments arranged by size:
4375 573 475 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2572 2721 3696 3781 4852 2572
Size: 149 975 85 1071 3355
Fragments arranged by size:
3355 1071 975 149 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2358 2572 2721 2753 2954 3696
Size: 399 556 12 214 149 32 201 742
Cuts at: 3696 3781 4852 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 214 201 149
85 32 12
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 2914 3524 5254 2914
Size: 610 1730 3295
Fragments arranged by size:
3295 1730 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2990 844
Size: 2146 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 2400 4279 4466 1700
Size: 110 506 84 1879 187 2869
Fragments arranged by size:
2869 1879 506 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 2134 4439 2134
Size: 2305 3330
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2472 2686 2965 3332 3717 3734 5186 5272
Size: 500 214 279 367 385 17 1452 86
Cuts at: 5272 5284 5630 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 500 385 367 346 279 214
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2476 513
Size: 552 1411 3672
Fragments arranged by size:
3672 1411 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 2430 1554
Size: 876 4759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2491 2939 2491
Size: 448 5187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2108 3082 3971
Size: 54 1425 50 275 18 278 974 889
Cuts at: 3971 5600 8
Size: 1629 43
Fragments arranged by size:
1629 1425 974 889 278 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2898 3565 1353
Size: 1269 276 667 3423
Fragments arranged by size:
3423 1269 667 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2719 2719
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2052 2081 2197 2244 2489 2556
Size: 393 368 52 29 116 47 245 67
Cuts at: 2556 2720 2919 3698 4295 5082 5350 1239
Size: 164 199 779 597 787 268 1524
Fragments arranged by size:
1524 787 779 597 393 368 268 245
199 164 116 67 52 47 29
EcoO109I rG'GnC_Cy
Cuts at: 2254 2876 2254
Size: 622 5013
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2646 2678 4276
Size: 32 135 32 825 32 217 32 1598
Cuts at: 4276 4308 4350 4382 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1598 825 217 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 2979 3225 669
Size: 988 281 772 269 246 3079
Fragments arranged by size:
3079 988 772 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2231 4749 4933 1305
Size: 926 2518 184 2007
Fragments arranged by size:
2518 2007 926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2919 3593 3952 4111 518
Size: 648 1753 674 359 159 2042
Fragments arranged by size:
2042 1753 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3051 3728 4876 5121 1606
Size: 1445 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 677 245
NaeI GCC'GGC
Cuts at: 2402 2402
Size: 5635
NcoI C'CATG_G
Cuts at: 513 1065 2476 513
Size: 552 1411 3672
Fragments arranged by size:
3672 1411 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2400 2400
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 2579 1108
Size: 1149 322 4164
Fragments arranged by size:
4164 1149 322
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2876 2254
Size: 622 5013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SacI G_AGCT'C
Cuts at: 2721 2721
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SanDI GG'GwC_CC
Cuts at: 2876 2876
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2983 4118 4799 4990 826
Size: 219 1938 1135 681 191 1471
Fragments arranged by size:
1938 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2294 2962 1872
Size: 422 668 4545
Fragments arranged by size:
4545 668 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2586 3739 4640 4902 820
Size: 197 926 643 1153 901 262 1553
Fragments arranged by size:
1553 1153 926 901 643 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2476 3074 513
Size: 552 1411 598 3074
Fragments arranged by size:
3074 1411 598 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 3662 3679 3832 4017 4462 325
Size: 1255 855 1227 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1227 855 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 5595 5627 2273
Size: 32 3290 32 2281
Fragments arranged by size:
3290 2281 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2051 2464 2879 2051
Size: 413 415 4807
Fragments arranged by size:
4807 415 413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2292 2960 1870
Size: 422 668 4545
Fragments arranged by size:
4545 668 422
XmnI GAAnn'nnTTC
Cuts at: 2855 3764 2855
Size: 909 4726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI