Restriction map for
pFN21AE0159
(Circular) (Six-base) MAPSORT of: pFN21AE0159.seq Check: 8748 from: 1 to: 5437
pFN21AE0159 5437 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2584 3500 4884 5152 1632
Size: 952 916 1384 268 1917
Fragments arranged by size:
1917 1384 952 916 268
AfeI AGC'GCT
Cuts at: 2152 2152
Size: 5437
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2272 1184
Size: 1088 4349
AlwNI CAG_nnn'CTG
Cuts at: 2494 2607 3180 4752 2494
Size: 113 573 1572 3179
Fragments arranged by size:
3179 1572 573 113
ApaI G_GGCC'C
Cuts at: 2101 2101
Size: 5437
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2621 2746 2972 3124 5233 1493
Size: 1128 125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1128 226 152 125
AscI GG'CGCG_CC
Cuts at: 2033 2033
Size: 5437
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2522 2762 1870
Size: 73 579 240 4545
Fragments arranged by size:
4545 579 240 73
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2758 4213 618
Size: 325 217 627 971 1455 1842
Fragments arranged by size:
1842 1455 971 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2101 2675 2756 1391
Size: 710 574 81 4072
Fragments arranged by size:
4072 710 574 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BbvCI CC'TCA_GC
Cuts at: 2211 2211
Size: 5437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2075 2109 3628 3662 1903
Size: 34 138 34 1519 34 3678
Fragments arranged by size:
3678 1519 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2554 3329 4539 2554
Size: 775 1210 3452
Fragments arranged by size:
3452 1210 775
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4048 136
Size: 107 122 71 631 605 337 2039 1525
Fragments arranged by size:
2039 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2101 3498 4654 1790
Size: 311 1397 1156 2573
Fragments arranged by size:
2573 1397 1156 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2157 1056
Size: 1101 4336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2341 4097 1239
Size: 1047 55 1756 2579
Fragments arranged by size:
2579 1756 1047 55
Bpu10I CC'TnA_GC
Cuts at: 2211 2674 2211
Size: 463 4974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 3562 4427 4725 2366
Size: 1196 865 298 3078
Fragments arranged by size:
3078 1196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 3997
Fragments arranged by size:
3997 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 2498 2725 4640 5136 2498
Size: 227 1915 496 2799
Fragments arranged by size:
2799 1915 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 1163
Size: 573 369 4495
Fragments arranged by size:
4495 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2669 2887 3648 4676 1540
Size: 275 186 668 218 761 1028 2301
Fragments arranged by size:
2301 1028 761 668 275 218 186
BsiHKAI G_wGCw'C
Cuts at: 2547 2675 3498 3583 4654 2547
Size: 128 823 85 1071 3330
Fragments arranged by size:
3330 1071 823 128 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2547 2675 2756 3498 3583 4654
Size: 399 311 446 128 81 742 85 1071
Cuts at: 4654 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 446 399 311 128 85
81
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 844
Size: 1948 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 3932 4106 65
Size: 1183 1042 1642 174 1396
Fragments arranged by size:
1642 1396 1183 1042 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 2033 1925
Size: 108 5329
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2420 794
Size: 1626 3811
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 2551 2695 1184
Size: 1367 144 3926
Fragments arranged by size:
3926 1367 144
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432
Size: 795 367 385 17 1452 86 12 346
Cuts at: 5432 1972
Size: 1977
Fragments arranged by size:
1977 1452 795 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2589 2589
Size: 5437
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2521 487
Size: 683 56 1295 3403
Fragments arranged by size:
3403 1295 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 2884 3773
Size: 54 1425 50 275 18 630 424 889
Cuts at: 3773 5402 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 630 424 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2229 3367 1353
Size: 783 93 1138 3423
Fragments arranged by size:
3423 1138 783 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2673 2673
Size: 5437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2341 2584 3500 4097 4884 5152
Size: 393 654 55 243 916 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 916 787 654 597 393 268 243
55
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 2687
Size: 5437
EcoRI G'AATT_C
Cuts at: 2621 2746 3124 2621
Size: 125 378 4934
Fragments arranged by size:
4934 378 125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 5437
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2067 2154 4584 1678
Size: 389 87 2430 2531
Fragments arranged by size:
2531 2430 389 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 4078 4110 4152
Size: 32 135 32 1016 32 1458 32 42
Cuts at: 4152 4184 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 1016 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2197 4551 4735 1305
Size: 892 2354 184 2007
Fragments arranged by size:
2354 2007 892 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 2985
Fragments arranged by size:
2985 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3395 3754 3913 518
Size: 648 1374 855 359 159 2042
Fragments arranged by size:
2042 1374 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2511 2853 3530 4678 4923 1606
Size: 905 342 677 1148 245 2120
Fragments arranged by size:
2120 1148 905 677 342 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3946
Fragments arranged by size:
3946 939 552
NdeI CA'TA_TG
Cuts at: 387 2339 387
Size: 1952 3485
NheI G'CTAG_C
Cuts at: 1052 2153 1052
Size: 1101 4336
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2518 2795 4340 1132
Size: 97 1289 277 1545 2229
Fragments arranged by size:
2229 1545 1289 277 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2494 1108
Size: 1386 4051
PfoI T'CCnGG_A
Cuts at: 2606 2606
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2097 2097
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2026 2789 830
Size: 1196 763 3478
Fragments arranged by size:
3478 1196 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SacI G_AGCT'C
Cuts at: 2675 2675
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SexAI A'CCwGG_T
Cuts at: 2199 2199
Size: 5437
SfcI C'TryA_G
Cuts at: 826 1045 2022 2785 3920 4601 4792 826
Size: 219 977 763 1135 681 191 1471
Fragments arranged by size:
1471 1135 977 763 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2524 2764 1872
Size: 652 240 4545
Fragments arranged by size:
4545 652 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2381 3541 4442 4704 820
Size: 197 926 438 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 438 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2518 2795 1132
Size: 1386 277 3774
Fragments arranged by size:
3774 1386 277
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2394 1517
Size: 877 4560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 2463 2876 513
Size: 552 939 399 60 413 3074
Fragments arranged by size:
3074 939 552 413 399 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2683 3464 3481 3634 3819 4264
Size: 1255 639 464 781 17 153 185 445
Cuts at: 4264 325
Size: 1498
Fragments arranged by size:
1498 1255 781 639 464 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2495 2125
Size: 370 5067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2522 2762 1870
Size: 652 240 4545
Fragments arranged by size:
4545 652 240
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI