Restriction map for
pFN21AE0158
(Circular) (Six-base) MAPSORT of: pFN21AE0158.seq Check: 2433 from: 1 to: 5581
pFN21AE0158 5581 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 1937
Size: 987 4594
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2700 2762 3644 5028 5296 1632
Size: 743 325 62 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 743 325 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2322 1184
Size: 1138 4443
AlwNI CAG_nnn'CTG
Cuts at: 3324 4896 3324
Size: 1572 4009
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2235 2906 1870
Size: 73 175 117 671 4545
Fragments arranged by size:
4545 671 175 117 73
BamHI G'GATC_C
Cuts at: 2528 2911 3278 2528
Size: 383 367 4831
Fragments arranged by size:
4831 383 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2902 4357 618
Size: 325 217 627 1115 1455 1842
Fragments arranged by size:
1842 1455 1115 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 1391
Size: 1509 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 928
Size: 971 909 3701
Fragments arranged by size:
3701 971 909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3772 3806 1903
Size: 34 1835 34 3678
Fragments arranged by size:
3678 1835 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2747 3473 4683 2747
Size: 726 1210 3645
Fragments arranged by size:
3645 1210 726
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4192 136
Size: 107 122 71 631 605 2520 1525
Fragments arranged by size:
2520 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2773 5576 2773
Size: 2803 2778
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2175 3642 4798 1790
Size: 329 56 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 329 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 3312 4270
Size: 416 53 83 399 24 754 1141 958
Cuts at: 4270 442
Size: 1753
Fragments arranged by size:
1753 1141 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 4241 1239
Size: 1233 1769 2579
Fragments arranged by size:
2579 1769 1233
Bpu10I CC'TnA_GC
Cuts at: 2378 2598 2378
Size: 220 5361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 4244 882
Size: 617 627 2118 2219
Fragments arranged by size:
2219 2118 627 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 2735 5575 2735
Size: 2840 2741
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3388 3770 4405
Size: 53 83 186 936 590 1265 382 635
Cuts at: 4405 275
Size: 1451
Fragments arranged by size:
1451 1265 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2781 4014 4412 4686 4833 5244 2781
Size: 1233 398 274 147 411 3118
Fragments arranged by size:
3118 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2351 2591 1186
Size: 1051 114 240 4176
Fragments arranged by size:
4176 1051 240 114
BseYI C'CCAG_C
Cuts at: 2279 2407 4784 5280 2279
Size: 128 2377 496 2580
Fragments arranged by size:
2580 2377 496 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5008
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3642 3727 4798 3642
Size: 85 1071 4425
Fragments arranged by size:
4425 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
BsmBI CGTCTCn'nnnn_
Cuts at: 2701 2701
Size: 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2175 2900 3642 3727 4798 1391
Size: 399 329 56 725 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 329 85 56
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 2936 844
Size: 1487 605 3489
Fragments arranged by size:
3489 1487 605
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 4076 4250 65
Size: 1183 1063 1765 174 1396
Fragments arranged by size:
1765 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4225 4412 1700
Size: 110 2415 187 2869
Fragments arranged by size:
2869 2415 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 3641 4653 3641
Size: 1012 4569
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2450 794
Size: 1656 3925
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2528 2773 2911 3278 3663 3680 5132 5218
Size: 556 245 138 367 385 17 1452 86
Cuts at: 5218 5230 5576 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 556 385 367 346 245 138
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2831 2831
Size: 5581
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2182 487
Size: 683 56 956 3886
Fragments arranged by size:
3886 956 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2308 2343 2421 2456 529
Size: 35 1744 35 78 35 3654
Fragments arranged by size:
3654 1744 78 35 35 35
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546
Size: 54 1425 50 275 18 1198 889 1629
Cuts at: 5546 8
Size: 43
Fragments arranged by size:
1629 1425 1198 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2512 2676 3511 1353
Size: 861 298 164 835 3423
Fragments arranged by size:
3423 861 835 298 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2472 2700 2762 3644 4241 5028
Size: 393 743 97 228 62 882 597 787
Cuts at: 5028 5296 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 743 597 393 268 228
97 62
EcoO109I rG'GnC_Cy
Cuts at: 2593 2827 2593
Size: 234 5347
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
EcoRV GAT'ATC
Cuts at: 2863 2863
Size: 5581
FspI TGC'GCA
Cuts at: 2420 4087 2420
Size: 1667 3914
HaeII r_GCGC'y
Cuts at: 1678 4728 1678
Size: 3050 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2591 2623 2681
Size: 32 135 32 896 32 91 32 58
Cuts at: 2681 2713 2758 2790 4222 4254 4296 4328 1373
Size: 32 45 32 1432 32 42 32 2626
Fragments arranged by size:
2626 1432 896 135 91 58 45 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2925 3171 669
Size: 988 281 987 246 3079
Fragments arranged by size:
3079 988 987 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3759
Fragments arranged by size:
3759 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3539 3898 4057 518
Size: 648 2373 359 159 2042
Fragments arranged by size:
2373 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2447 2997 3674 4822 5067 1606
Size: 841 550 677 1148 245 2120
Fragments arranged by size:
2120 1148 841 677 550 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5029
NdeI CA'TA_TG
Cuts at: 387 2230 387
Size: 1843 3738
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2939 4484 1132
Size: 97 1710 1545 2229
Fragments arranged by size:
2229 1710 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PfoI T'CCnGG_A
Cuts at: 2488 2488
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2593 2827 2593
Size: 234 5347
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2400 2672 2933 830
Size: 1570 272 261 3478
Fragments arranged by size:
3478 1570 272 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2447 2447
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2396 2668 2929 4064 4745 4936 826
Size: 219 1351 272 261 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 272 261 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2120 2237 2908 1872
Size: 248 117 671 4545
Fragments arranged by size:
4545 671 248 117
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3685 4586 4848 820
Size: 197 926 1742 901 262 1553
Fragments arranged by size:
1742 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SrfI GCCC'GGGC
Cuts at: 2120 2120
Size: 5581
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2706 3020 513
Size: 552 1641 314 3074
Fragments arranged by size:
3074 1641 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3608 3625 3778 3963 4408 325
Size: 1255 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5541 5573 5541
Size: 32 5549
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 1316
Size: 1377 4204
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2118 2235 2906 1870
Size: 248 117 671 4545
Fragments arranged by size:
4545 671 248 117
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SwaI